The objective of the study is the cytocidal and inhibitory effect of energy-controllable pulse on ovarian cancer cell line SKOV3. Ovarian cancer cell suspension were treated by electric pulse with different parameters...The objective of the study is the cytocidal and inhibitory effect of energy-controllable pulse on ovarian cancer cell line SKOV3. Ovarian cancer cell suspension were treated by electric pulse with different parameters. The inhibitory rate (IR) was assayed by modified colorimetric MTT methods, the growth curves of two test groups and one control group were also measured, and the ultrastructural changes were observed under electron microscopy (EM) and scan electron microscopy (SEM). It was found that the treated SKOV3 cell proliferated more slowly. IR was increased with the enhancement of pulse parameters. The ultrastructural study showed that morphological changes occurred obviously. Swollen mitochondria, fractured ridges, cyto-plasmic vacuoles and membrane holes appeared in most of the processed cells, and a part of bilayer membrane was ruptured. It is indicated that irreversible electric breakdown occurred in some of the treated cells, and the electric pulse could kill cancer cell and inhibit its recovery and growth.展开更多
Objective：To evaluate the probability and efficacy of endostatin-vascular endothelial growth inhibitor （VEGI） recombinant adenoviruses combined with dexamethasone on suppressing heterolamellar corneal transplantati...Objective：To evaluate the probability and efficacy of endostatin-vascular endothelial growth inhibitor （VEGI） recombinant adenoviruses combined with dexamethasone on suppressing heterolamellar corneal transplantation rejection. Methods： Heterolamellar corneal transplantation models were established in 64 New Zealand rabbits, which were randomized into 4 groups of 16 rabbits each. After the transplantation, all the 64 right eyes were injected subconjunctively with 0.2 ml saline （saline group）, 0. 1 ml AdCA13-hENDO-VEGI151 plus 0. 1 ml saline （AdCA13-hENDO-VEGI151 group）, 0. 1 ml Dexamethasone （DXM） plus 0.1 ml saline （DXM group）, 0. 1 ml AdCA13-hENDO-VEGI151 plus 0. 1 ml DXM （AdCA13-hENDO-VEGI151 combined with DXM group） with one time each 3 days for 10 times. Graft survival and ocular surface were observed for 6 weeks. The fusion protein expression was detected by immunohistochemistry 6 weeks after transplantation. Results： Both the CNV index, rejection index and the xenograft rejection rate in the AdCA13-hENDO-VEGI151 combined with DXM group were statistically lower than those in other groups. AdCA13-hENDO-VEGI151 combined with DXM group： 1. 375 0±0. 500 0, 2. 750 0 ±1. 843 9 and 6.25% respectively 6 week after keratoplasty; Saline group： 3. 437 5±0. 512 3, 8. 812 5± 1. 108 7, 100. 00%; AdCA13-hENDO-VEGI151 group： 2. 312 5±0. 478 7, 5. 625 0±0. 957 4, 62.50%; DXM group： 3. 000 0±0. 816 5, 5. 562 5±1. 315 0, 56.25% （P〈0.01）. Immunohistochemical staining showed the fusion protein expressed mainly in corneal epithelium. Conclusion： The fusion protein expressed by the recombinant adenovirus has significant effect on inhibiting neovascularization after heterolamellar corneal transplantation. The topical application of AdCA13-hENDO-VEGI151 combined with DXM suppressed effectively the postoperative xenograft rejection rate of heterolamellar corneal transplantation.展开更多
Background: Over the past years, scientists have been working on the mechanisms of the scarless healing. The remarkable phenotypic differences between fetal and adult healing may lead us to find out their characterist...Background: Over the past years, scientists have been working on the mechanisms of the scarless healing. The remarkable phenotypic differences between fetal and adult healing may lead us to find out their characteristics in genetics, which represent potentially important mechanisms to explain the differences in the quality of wound repair observed in fetus versus adult tissues. Methods: Middle laparotomy and hysterotomy were performed on pregnant rabbits on 20-day gestation to expose the fetal back, and longitudinal incision which penetrated full skin was made on the back of fetus. The trauma fetus skin was harvested at 12 h post-operation (FT), the fetus control (FC) and trauma adult skin (AT) were taken at the same time. dscDNA was synthesized from total RNA of skin samples with SMART technology. An improved suppression subtractive hybridization (SSH) method was applied to analyze the samples. Having taken one of the three samples as Tester respectively, the other two together as Drivers, one forward and two reverse hybridization products were gotten. Having amplified by selective PCR, the products were inserted into vector, and then transferred into E.coli HB101. The colonies were screened by electrophoresis, reverse Northern afterwards, and the positive clones were sequenced. BLAST in NCBI was performed to compare and analyze the positive clones (expressed sequence Tag, ESTs). Results: Totally 298 clones were gotten and 61 positive clones were obtained after screening. The 61 selected positive clones were sequenced and 54 sequences were goten. Conclusion: Instead of traditional SSH, an improved SSH with 2 Drivers was applied in the experiment. The improved program is reasonable and correct in both theory and practice.展开更多
文摘The objective of the study is the cytocidal and inhibitory effect of energy-controllable pulse on ovarian cancer cell line SKOV3. Ovarian cancer cell suspension were treated by electric pulse with different parameters. The inhibitory rate (IR) was assayed by modified colorimetric MTT methods, the growth curves of two test groups and one control group were also measured, and the ultrastructural changes were observed under electron microscopy (EM) and scan electron microscopy (SEM). It was found that the treated SKOV3 cell proliferated more slowly. IR was increased with the enhancement of pulse parameters. The ultrastructural study showed that morphological changes occurred obviously. Swollen mitochondria, fractured ridges, cyto-plasmic vacuoles and membrane holes appeared in most of the processed cells, and a part of bilayer membrane was ruptured. It is indicated that irreversible electric breakdown occurred in some of the treated cells, and the electric pulse could kill cancer cell and inhibit its recovery and growth.
基金Support by the National Natural Science Foundation of China (No. 30271391, 30170891) the 973 Program (the Development Program of National Key Basic Research Projects (2003CB514129)
文摘Objective：To evaluate the probability and efficacy of endostatin-vascular endothelial growth inhibitor （VEGI） recombinant adenoviruses combined with dexamethasone on suppressing heterolamellar corneal transplantation rejection. Methods： Heterolamellar corneal transplantation models were established in 64 New Zealand rabbits, which were randomized into 4 groups of 16 rabbits each. After the transplantation, all the 64 right eyes were injected subconjunctively with 0.2 ml saline （saline group）, 0. 1 ml AdCA13-hENDO-VEGI151 plus 0. 1 ml saline （AdCA13-hENDO-VEGI151 group）, 0. 1 ml Dexamethasone （DXM） plus 0.1 ml saline （DXM group）, 0. 1 ml AdCA13-hENDO-VEGI151 plus 0. 1 ml DXM （AdCA13-hENDO-VEGI151 combined with DXM group） with one time each 3 days for 10 times. Graft survival and ocular surface were observed for 6 weeks. The fusion protein expression was detected by immunohistochemistry 6 weeks after transplantation. Results： Both the CNV index, rejection index and the xenograft rejection rate in the AdCA13-hENDO-VEGI151 combined with DXM group were statistically lower than those in other groups. AdCA13-hENDO-VEGI151 combined with DXM group： 1. 375 0±0. 500 0, 2. 750 0 ±1. 843 9 and 6.25% respectively 6 week after keratoplasty; Saline group： 3. 437 5±0. 512 3, 8. 812 5± 1. 108 7, 100. 00%; AdCA13-hENDO-VEGI151 group： 2. 312 5±0. 478 7, 5. 625 0±0. 957 4, 62.50%; DXM group： 3. 000 0±0. 816 5, 5. 562 5±1. 315 0, 56.25% （P〈0.01）. Immunohistochemical staining showed the fusion protein expressed mainly in corneal epithelium. Conclusion： The fusion protein expressed by the recombinant adenovirus has significant effect on inhibiting neovascularization after heterolamellar corneal transplantation. The topical application of AdCA13-hENDO-VEGI151 combined with DXM suppressed effectively the postoperative xenograft rejection rate of heterolamellar corneal transplantation.
文摘Background: Over the past years, scientists have been working on the mechanisms of the scarless healing. The remarkable phenotypic differences between fetal and adult healing may lead us to find out their characteristics in genetics, which represent potentially important mechanisms to explain the differences in the quality of wound repair observed in fetus versus adult tissues. Methods: Middle laparotomy and hysterotomy were performed on pregnant rabbits on 20-day gestation to expose the fetal back, and longitudinal incision which penetrated full skin was made on the back of fetus. The trauma fetus skin was harvested at 12 h post-operation (FT), the fetus control (FC) and trauma adult skin (AT) were taken at the same time. dscDNA was synthesized from total RNA of skin samples with SMART technology. An improved suppression subtractive hybridization (SSH) method was applied to analyze the samples. Having taken one of the three samples as Tester respectively, the other two together as Drivers, one forward and two reverse hybridization products were gotten. Having amplified by selective PCR, the products were inserted into vector, and then transferred into E.coli HB101. The colonies were screened by electrophoresis, reverse Northern afterwards, and the positive clones were sequenced. BLAST in NCBI was performed to compare and analyze the positive clones (expressed sequence Tag, ESTs). Results: Totally 298 clones were gotten and 61 positive clones were obtained after screening. The 61 selected positive clones were sequenced and 54 sequences were goten. Conclusion: Instead of traditional SSH, an improved SSH with 2 Drivers was applied in the experiment. The improved program is reasonable and correct in both theory and practice.
