The gut microbiota is a complex ecosystem composed of many bacteria and their metabolites.It plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,immune regulation,and many o...The gut microbiota is a complex ecosystem composed of many bacteria and their metabolites.It plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,immune regulation,and many other aspects.Exploring the structure and function of the gut microbiota,as well as their key genes and metabolites,will enable the early diagnosis and auxiliary diagnosis of diseases,new treatment methods,better effects of drug treatments,and better guidance in the use of antibiotics.The identification of gut microbiota plays an important role in clinical diagnosis and treatment,as well as in drug research and development.Therefore,it is necessary to conduct a comprehensive review of this rapidly evolving topic.Traditional identification methods cannot comprehensively capture the diversity of gut microbiota.Currently,with the rapid development of molecular biology,the classification and identification methods for gut microbiota have evolved from the initial phenotypic and chemical identification to identification at the molecular level.This review integrates the main methods of gut microbiota identification and evaluates their application.We pay special attention to the research progress on molecular biological methods and focus on the application of high-throughput sequencing technology in the identification of gut microbiota.This revolutionary method for intestinal flora identification heralds a new chapter in our understanding of the microbial world.展开更多
In order to study the epidemiological characteristics of cytomegalovirus(CMV)infection in allogeneichematopoietic stem cell transplantation(allo-HSCT)recipients by means of plasma real time quantitative polymerasechai...In order to study the epidemiological characteristics of cytomegalovirus(CMV)infection in allogeneichematopoietic stem cell transplantation(allo-HSCT)recipients by means of plasma real time quantitative polymerasechain reaction(RQ-PCR),141 adult patients undergoing allo-HSCT between January 2008 and June 2010 were seriallymonitored by RQ-PCR for detecting CMV and guiding the preemptive therapy followed up to 180 days post-HSCT.Theresults showed that the incidence of CMV infection and CMV pneumonia was 81.5%and 2.9%respectively,whichmainly occurred within 2 months post-HSCT.Single-therapy with ganciclovir(GCV)for 63 patients or foscarnet 6patients was performed for preemptive therapy.The total efficacy was 87.8%,and the response patterns were different.CMV infection was more frequent in female patients(P=0.044),and those with aGVHD(P=0.043),using ATG orbasiliximab in conditioning regimens(P=0.049),as well as earlier in patients using ATG or basiliximab or those withaGVHD(P=0.007;P=0.000).The aGVHD,maximum load,positive times of CMV-DNA detection and therapyduration all correlated with the efficacy(P<0.05).It is concluded that the incidence of CMV infection is still high afterHSCT.Plasma RQ-PCR assay for CMV-DNA shows a strong correlation with the clinical outcome of CMV infection,which is useful and suitable for management of CMV infection in HSCT.展开更多
基金The National Natural Science Foundation of China(31071775)The Science and Technology Department of Zhejiang Province(2008C24006)The Zhejiang Normal University Innovative Research Team Program
文摘The gut microbiota is a complex ecosystem composed of many bacteria and their metabolites.It plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,immune regulation,and many other aspects.Exploring the structure and function of the gut microbiota,as well as their key genes and metabolites,will enable the early diagnosis and auxiliary diagnosis of diseases,new treatment methods,better effects of drug treatments,and better guidance in the use of antibiotics.The identification of gut microbiota plays an important role in clinical diagnosis and treatment,as well as in drug research and development.Therefore,it is necessary to conduct a comprehensive review of this rapidly evolving topic.Traditional identification methods cannot comprehensively capture the diversity of gut microbiota.Currently,with the rapid development of molecular biology,the classification and identification methods for gut microbiota have evolved from the initial phenotypic and chemical identification to identification at the molecular level.This review integrates the main methods of gut microbiota identification and evaluates their application.We pay special attention to the research progress on molecular biological methods and focus on the application of high-throughput sequencing technology in the identification of gut microbiota.This revolutionary method for intestinal flora identification heralds a new chapter in our understanding of the microbial world.
基金Key Program of Capital Development Foundation(编号2007-2040)
文摘In order to study the epidemiological characteristics of cytomegalovirus(CMV)infection in allogeneichematopoietic stem cell transplantation(allo-HSCT)recipients by means of plasma real time quantitative polymerasechain reaction(RQ-PCR),141 adult patients undergoing allo-HSCT between January 2008 and June 2010 were seriallymonitored by RQ-PCR for detecting CMV and guiding the preemptive therapy followed up to 180 days post-HSCT.Theresults showed that the incidence of CMV infection and CMV pneumonia was 81.5%and 2.9%respectively,whichmainly occurred within 2 months post-HSCT.Single-therapy with ganciclovir(GCV)for 63 patients or foscarnet 6patients was performed for preemptive therapy.The total efficacy was 87.8%,and the response patterns were different.CMV infection was more frequent in female patients(P=0.044),and those with aGVHD(P=0.043),using ATG orbasiliximab in conditioning regimens(P=0.049),as well as earlier in patients using ATG or basiliximab or those withaGVHD(P=0.007;P=0.000).The aGVHD,maximum load,positive times of CMV-DNA detection and therapyduration all correlated with the efficacy(P<0.05).It is concluded that the incidence of CMV infection is still high afterHSCT.Plasma RQ-PCR assay for CMV-DNA shows a strong correlation with the clinical outcome of CMV infection,which is useful and suitable for management of CMV infection in HSCT.
