目的:建立幼年海人酸颞叶癫痫模型并给予平痫冲剂治疗,探讨对其学习记忆行为的影响。方法:将60只40日龄Wi st ar大鼠,随机分为正常对照组(A组)、模型组(B组)、阳性对照组(C组)及平痫冲剂低(D组)、中(E组)、高剂量组(F组)。除A组外,其余...目的:建立幼年海人酸颞叶癫痫模型并给予平痫冲剂治疗,探讨对其学习记忆行为的影响。方法:将60只40日龄Wi st ar大鼠,随机分为正常对照组(A组)、模型组(B组)、阳性对照组(C组)及平痫冲剂低(D组)、中(E组)、高剂量组(F组)。除A组外,其余各组脑立体定位注射海人酸诱发颞叶癫痫。A组正常饲养,B组灌服等量生理盐水,D、E、F组灌服平痫冲剂,低、中、高给药剂量按人(70 kg)用药剂量的3倍、6倍、12倍给药,分别为0.7、1.4、2.8 g/(kg.d),C组灌服等量卡马西平,均每日灌胃1次,连续灌胃1月。实验过程中监测大鼠症状的改变,深部脑电图观察颞叶癫痫的病理过程及治疗前后的脑电变化,利用Morri s水迷宫和八臂迷宫实验评价各组大鼠的学习记忆行为。结果:①大鼠症状的改变:海人酸注射后半小时到6小时大鼠出现急性发作,进而逐渐缓减,2个月后出现自发发作。平痫冲剂治疗可抑制癫痫发作及其发展;②电生理学改变:脑电图于24小时和2个月后均表现为典型的癫痫发作电活动及发作间期表现。治疗后癫痫波持续时间缩短;③学习记忆行为的变化:海人酸颞叶癫痫大鼠学习记忆能力明显降低。平痫冲剂可缩短其逃避潜伏期,提高穿越原平台次数,减少参考记忆和工作记忆错误。结论:海人酸模型是模拟人类颞叶癫痫较为理想的动物模型;平痫冲剂对癫痫发作及脑电活动具有抑制作用,并且可以改善其学习记忆行为能力。展开更多
Objective: To investigate the effect of behavior training on the learning and memory of young rats with fetal growth restriction (FGR). Methods: The model of FGR was established by passive smoking method to pregnant r...Objective: To investigate the effect of behavior training on the learning and memory of young rats with fetal growth restriction (FGR). Methods: The model of FGR was established by passive smoking method to pregnant rats. The new-born rats were divided into FGR group and normal group, and then randomly subdivided into trained and untrained group respectively. Morris water maze behavior training was performed on postnatal months 2 and 4, then learning and memory abilities of young rats were measured by dark-avoidance testing and step-down testing. Results: In the dark-avoidance and step-down testing, the young rats’ performance of FGR group was worse than that of control group, and the trained group was better than the untrained group significantly. Conclusion: FGR young rats have descended learning and memory abilities. Behavior training could improve the young rats’ learning and memory abilities, especially for the FGR young rats.展开更多
基金the National Natural Science Foundation of China(30471826)
文摘Objective: To investigate the effect of behavior training on the learning and memory of young rats with fetal growth restriction (FGR). Methods: The model of FGR was established by passive smoking method to pregnant rats. The new-born rats were divided into FGR group and normal group, and then randomly subdivided into trained and untrained group respectively. Morris water maze behavior training was performed on postnatal months 2 and 4, then learning and memory abilities of young rats were measured by dark-avoidance testing and step-down testing. Results: In the dark-avoidance and step-down testing, the young rats’ performance of FGR group was worse than that of control group, and the trained group was better than the untrained group significantly. Conclusion: FGR young rats have descended learning and memory abilities. Behavior training could improve the young rats’ learning and memory abilities, especially for the FGR young rats.
