Curcumin is the main component of Curcuma longa,a traditional Chinese medicinal herb,with excellent antioxidant.Curcumin is easily decomposed under light and heat.In this study,to find stable antioxidants,6 curcumin a...Curcumin is the main component of Curcuma longa,a traditional Chinese medicinal herb,with excellent antioxidant.Curcumin is easily decomposed under light and heat.In this study,to find stable antioxidants,6 curcumin analogs(A1-6) were synthesized by coupling the appropriate aromatic aldehyde and cyclohexanon with HCl catalysis,and the free radical（DPPH） scavenging abilities of synthetic curcumin analogs were evaluated.The results showed that the free radical（DPPH） scavenging abilities of curcumin analogs（A2,A3 and A6） were promoted when hydroxyl or methoxyl groups were introduced in the ortho place of phenols,the compound with ortho dihydroxy groups showed the stronges free radical（DPPH） scavenging abilities among curcumin analogs with IC50 of 1.5 μM.展开更多
在B3LYP/6-31G(d,p)水平下优化了四种姜黄素类似物的几何构型,并通过振动分析验证了其构型稳定性。在B3LYP/6-311G(d,p)水平下计算了该类化合物的核磁共振谱,研究结果表明:四种化合物主体结构共平面性较好,为一较大共轭体系。由于羟基...在B3LYP/6-31G(d,p)水平下优化了四种姜黄素类似物的几何构型,并通过振动分析验证了其构型稳定性。在B3LYP/6-311G(d,p)水平下计算了该类化合物的核磁共振谱,研究结果表明:四种化合物主体结构共平面性较好,为一较大共轭体系。由于羟基及甲氧基的引入,使Compound-B/C/D中C3,C4以及Compound-A/D中C5均具有较大δ值,Compound-A中C4和C6的δ值相对较小,C3的δ值相对较大。而在羰基与碳碳双键所形成的共轭羰基化合物中,羰基C13的δ值(183ppm)相对于乙醛中的δ值(201ppm)有所减小,C11,15(α碳)的δ值(122ppm)相对减小,而C9,17(β碳)的δ值(145ppm)相对增大。最后,通过线性回归方法,利用相关系数值r研究了1 H NMRδ值的实验和理论计算值的相关性,结果表明相关性较好,实验值和理论值基本吻合。展开更多
文摘Curcumin is the main component of Curcuma longa,a traditional Chinese medicinal herb,with excellent antioxidant.Curcumin is easily decomposed under light and heat.In this study,to find stable antioxidants,6 curcumin analogs(A1-6) were synthesized by coupling the appropriate aromatic aldehyde and cyclohexanon with HCl catalysis,and the free radical（DPPH） scavenging abilities of synthetic curcumin analogs were evaluated.The results showed that the free radical（DPPH） scavenging abilities of curcumin analogs（A2,A3 and A6） were promoted when hydroxyl or methoxyl groups were introduced in the ortho place of phenols,the compound with ortho dihydroxy groups showed the stronges free radical（DPPH） scavenging abilities among curcumin analogs with IC50 of 1.5 μM.
文摘目的:探讨姜黄素类似物对糖尿病肾病(DN)小鼠肾纤维化的影响及机制。方法:KKAy小鼠给予高脂饲料连续饲养2周构建DN小鼠模型,随机分为模型组和实验组,每组12只,10只C57BL/6J小鼠饲喂普通饲料,作为正常组。实验组小鼠灌胃姜黄素类似物H85 mg/kg,模型组和正常组均灌胃等量生理盐水,1次/d,连续干预6周。采用血糖仪检测小鼠空腹血糖(FBG),双缩脲法检测处死前24 h尿液尿蛋白(UP);过碘酸-无色品红染色(PAS染色)观察小鼠肾组织病理形态变化;蛋白免疫印迹(WB)法及实时荧光定量-聚合酶链式反应(qPCR)检测各组小鼠肾组织α-平滑肌肌动蛋白(α-SMA)、纤连蛋白(FN)和IV型胶原(Col-Ⅳ)、Twist蛋白表达情况。结果:干预后,正常组、模型组和实验组小鼠肾脏指数(KI)分别为(6.24±0.32)、(14.4±2.39)、(9.14±1.43)mg/g,FBG分别为(4.96±1.25),(35.54±3.43),(27.54±1.12)mmol/L,24 h UP分别为(31.69±5.51)、(710.54±231.28)、(410.43±130.36)μmol/L;肾小球硬化评分分别为(0.00±0.00)、(3.02±0.24)、(1.69±0.21)分,组间差异有统计学意义(P<0.05)。与正常组比较,模型组小鼠肾组织α-SMA、FN和Col-Ⅳ、Twist蛋白及mRNA相对表达量升高(P<0.05),而实验组小鼠低于模型组(P<0.05)。结论:姜黄素类似物可改善DN小鼠肾功能及肾组织损伤程度,降低纤维化蛋白表达,延缓肾组织纤维化,可能与下调Twist蛋白表达有关。
文摘在B3LYP/6-31G(d,p)水平下优化了四种姜黄素类似物的几何构型,并通过振动分析验证了其构型稳定性。在B3LYP/6-311G(d,p)水平下计算了该类化合物的核磁共振谱,研究结果表明:四种化合物主体结构共平面性较好,为一较大共轭体系。由于羟基及甲氧基的引入,使Compound-B/C/D中C3,C4以及Compound-A/D中C5均具有较大δ值,Compound-A中C4和C6的δ值相对较小,C3的δ值相对较大。而在羰基与碳碳双键所形成的共轭羰基化合物中,羰基C13的δ值(183ppm)相对于乙醛中的δ值(201ppm)有所减小,C11,15(α碳)的δ值(122ppm)相对减小,而C9,17(β碳)的δ值(145ppm)相对增大。最后,通过线性回归方法,利用相关系数值r研究了1 H NMRδ值的实验和理论计算值的相关性,结果表明相关性较好,实验值和理论值基本吻合。
