提出了高效液相色谱-串联质谱法测定葛根中葛根素和大豆苷元的含量。样品经乙醇提取,所得提取液用乙醇定容至100mL后经Waters Xterra MS C18色谱柱(150mm×3.9mm,5μm)分离,用乙腈与50mmol.L-1甲酸溶液(40+60)的混合液洗脱,采用电...提出了高效液相色谱-串联质谱法测定葛根中葛根素和大豆苷元的含量。样品经乙醇提取,所得提取液用乙醇定容至100mL后经Waters Xterra MS C18色谱柱(150mm×3.9mm,5μm)分离,用乙腈与50mmol.L-1甲酸溶液(40+60)的混合液洗脱,采用电喷雾正离子电离多反应监测模式。葛根素和大豆苷元的质量浓度分别在0.050～0.50mg.L-1和5.0～50mg.L-1之间与峰面积呈线性关系,检出限(3S/N)均为5μg.L-1。在0.1,1.0,10.0mg.L-1 3个浓度水平进行加标回收试验,葛根素和大豆苷元的回收率分别为96.6%和97.4%。展开更多
Azido-3′-deoxythymidine(AZT) was the first clinically approved drug against HIV infection, despite its undesirable side reactions, such as bone marrow suppression. In our aim to develop new chemical entity of anti-tu...Azido-3′-deoxythymidine(AZT) was the first clinically approved drug against HIV infection, despite its undesirable side reactions, such as bone marrow suppression. In our aim to develop new chemical entity of anti-tumor and anti-HIV, the H-phosphonate 6 of AZT conjugate with diosgenin was synthesized by a tandem transesterification reaction for the first time. There are the merits of easy operation and high yield in the reported method. It could be extended to synthesize other diosgenin phosphonate conjugates such as carbohydrate and peptide.展开更多
文摘提出了高效液相色谱-串联质谱法测定葛根中葛根素和大豆苷元的含量。样品经乙醇提取,所得提取液用乙醇定容至100mL后经Waters Xterra MS C18色谱柱(150mm×3.9mm,5μm)分离,用乙腈与50mmol.L-1甲酸溶液(40+60)的混合液洗脱,采用电喷雾正离子电离多反应监测模式。葛根素和大豆苷元的质量浓度分别在0.050～0.50mg.L-1和5.0～50mg.L-1之间与峰面积呈线性关系,检出限(3S/N)均为5μg.L-1。在0.1,1.0,10.0mg.L-1 3个浓度水平进行加标回收试验,葛根素和大豆苷元的回收率分别为96.6%和97.4%。
文摘Azido-3′-deoxythymidine(AZT) was the first clinically approved drug against HIV infection, despite its undesirable side reactions, such as bone marrow suppression. In our aim to develop new chemical entity of anti-tumor and anti-HIV, the H-phosphonate 6 of AZT conjugate with diosgenin was synthesized by a tandem transesterification reaction for the first time. There are the merits of easy operation and high yield in the reported method. It could be extended to synthesize other diosgenin phosphonate conjugates such as carbohydrate and peptide.
