据Yan Y 2024年2月1日[Cell Stem Cell,2024,31(2):260-274.e7.]报道,美国威斯康星大学麦迪逊分校等机构研究人员通过研究开发出了首个3D打印的脑组织,其或能像典型的大脑组织一样生长和发挥功能;这一成果能帮助科学家们深入研究人类大...据Yan Y 2024年2月1日[Cell Stem Cell,2024,31(2):260-274.e7.]报道,美国威斯康星大学麦迪逊分校等机构研究人员通过研究开发出了首个3D打印的脑组织,其或能像典型的大脑组织一样生长和发挥功能;这一成果能帮助科学家们深入研究人类大脑并开发治疗一系列神经和神经发育障碍等疾病的新型疗法,比如阿尔兹海默病和帕金森等。该模型能帮助人们理解人类机体中脑细胞和部分大脑之间是如何交流的,同时也可能改变人们对干细胞生物学.展开更多
Objective: To investigate the roles of chloramphenicol (CAP) preconditioning in the oxidative respiratory function of cerebral mitochondria in rats exposed to acute hypoxia during acute hypoxia by observing the change...Objective: To investigate the roles of chloramphenicol (CAP) preconditioning in the oxidative respiratory function of cerebral mitochondria in rats exposed to acute hypoxia during acute hypoxia by observing the changes of mitochondrial oxidative respiratory function and cytochrome C oxidase (COX) activity. Methods: Adult male Wistar rats were randomly divided into 4 groups: control (C), medication (M), hypoxia (H), and medication plus hypoxia (MH). Rats in groups M and MH were administered by peritoneal injection of CAP (50 mg/kg) every 12 h for 7 d before decapitation, but those in groups H and MH were exposed to a hypobaric chamber simulating 5 000 m high altitude for 24 h. The rat cerebral cortex was removed and mitochondria were isolated by centrifugation. Mitochondrial respiratory function and COX activity were measured by Clark oxygen electrode. Results: Compared with Group C, Group H showed significantly elevated state 4 respiration (ST 4), decreased state 3 respiration (ST 3), and respiratory control rate (RCR) in mitochondrial respiration during acute hypoxic exposure. ST 3 in Group MH was significantly lower than that in Group C, but was not significantly different from that in Groups H and M, while ST 4 in Group MH was significantly lower than that in groups C and H. RCR in Group MH was higher than that in Group H, but lower than that in Group C. COX activity in Group H was significantly lower than that in Group C. In Group MH, COX activity increased and was higher than that in Group H, but was still lower than that in Group C. Conclusion: Acute hypoxic exposure could lead to mitochondrial respiratory dysfunction, suggesting that CAP preconditioning might be beneficial to the recovery of rat respiratory function. The change of COX activity is consistent with that of mitochondrial respiratory function during acute hypoxic exposure and CAP-administration, indicating that COX plays an important role in oxidative phosphorylation function of mitochondria from cerebral cortex of hypoxic rats.展开更多
文摘据Yan Y 2024年2月1日[Cell Stem Cell,2024,31(2):260-274.e7.]报道,美国威斯康星大学麦迪逊分校等机构研究人员通过研究开发出了首个3D打印的脑组织,其或能像典型的大脑组织一样生长和发挥功能;这一成果能帮助科学家们深入研究人类大脑并开发治疗一系列神经和神经发育障碍等疾病的新型疗法,比如阿尔兹海默病和帕金森等。该模型能帮助人们理解人类机体中脑细胞和部分大脑之间是如何交流的,同时也可能改变人们对干细胞生物学.
文摘Objective: To investigate the roles of chloramphenicol (CAP) preconditioning in the oxidative respiratory function of cerebral mitochondria in rats exposed to acute hypoxia during acute hypoxia by observing the changes of mitochondrial oxidative respiratory function and cytochrome C oxidase (COX) activity. Methods: Adult male Wistar rats were randomly divided into 4 groups: control (C), medication (M), hypoxia (H), and medication plus hypoxia (MH). Rats in groups M and MH were administered by peritoneal injection of CAP (50 mg/kg) every 12 h for 7 d before decapitation, but those in groups H and MH were exposed to a hypobaric chamber simulating 5 000 m high altitude for 24 h. The rat cerebral cortex was removed and mitochondria were isolated by centrifugation. Mitochondrial respiratory function and COX activity were measured by Clark oxygen electrode. Results: Compared with Group C, Group H showed significantly elevated state 4 respiration (ST 4), decreased state 3 respiration (ST 3), and respiratory control rate (RCR) in mitochondrial respiration during acute hypoxic exposure. ST 3 in Group MH was significantly lower than that in Group C, but was not significantly different from that in Groups H and M, while ST 4 in Group MH was significantly lower than that in groups C and H. RCR in Group MH was higher than that in Group H, but lower than that in Group C. COX activity in Group H was significantly lower than that in Group C. In Group MH, COX activity increased and was higher than that in Group H, but was still lower than that in Group C. Conclusion: Acute hypoxic exposure could lead to mitochondrial respiratory dysfunction, suggesting that CAP preconditioning might be beneficial to the recovery of rat respiratory function. The change of COX activity is consistent with that of mitochondrial respiratory function during acute hypoxic exposure and CAP-administration, indicating that COX plays an important role in oxidative phosphorylation function of mitochondria from cerebral cortex of hypoxic rats.
