Erythropoietin (EPO) is a glycoprotein with three N-glycosylation sites and one O-glycosylation sites. According to the reference, all of the N-glyco-chains were acid complex type. The amount of sialic acid in rhEPO w...Erythropoietin (EPO) is a glycoprotein with three N-glycosylation sites and one O-glycosylation sites. According to the reference, all of the N-glyco-chains were acid complex type. The amount of sialic acid in rhEPO was determined and two N-glycosylation sites on it were confirmed by matrix-assisted laser desorption time of flight-time of flight mass spectrometry (MALDI-TOF-TOFMS) in this paper. The total amount of sialic acid in rhEPO accounts for 12.97%. Two N-glycosylation sites (Asn38,Asn83) were identified successfully.展开更多
近年来,基质辅助激光解析电离飞行时间质谱(matrix-assisted laser desorption ionization time of flight mass spectrometry,MALDI-TOF-MS)作为一种新的质谱技术成功检测出许多与疾病相关的新型生物标志物,尤其在肿瘤的诊治方面,MALDI...近年来,基质辅助激光解析电离飞行时间质谱(matrix-assisted laser desorption ionization time of flight mass spectrometry,MALDI-TOF-MS)作为一种新的质谱技术成功检测出许多与疾病相关的新型生物标志物,尤其在肿瘤的诊治方面,MALDITOF-MS可以快速检测出癌症患者体内特异的蛋白表达、辅助制定患者特异性的疗法、预测患者的治疗效果以及是否有复发的可能等。近年来,国内外学者开展了多项MALDI-TOF-MS在宫颈癌、子宫内膜癌、卵巢癌三大女性生殖道恶性肿瘤中的早期筛查诊断研究,其应用于宫颈癌的蛋白组学诊断已被证实具有实验可行性;其可以检测固态、液态等多种形态样本的特点,为寻求无创早期诊断的子宫内膜癌研究者提供了新的研究方法;其在卵巢癌差异蛋白检测、化疗耐药相关分子检测中发挥了重要作用。但仍存在如何提高MALDI-TOF-MS检测的灵敏度以及满足蛋白质组学研究高通量的需要等问题。展开更多
文摘Erythropoietin (EPO) is a glycoprotein with three N-glycosylation sites and one O-glycosylation sites. According to the reference, all of the N-glyco-chains were acid complex type. The amount of sialic acid in rhEPO was determined and two N-glycosylation sites on it were confirmed by matrix-assisted laser desorption time of flight-time of flight mass spectrometry (MALDI-TOF-TOFMS) in this paper. The total amount of sialic acid in rhEPO accounts for 12.97%. Two N-glycosylation sites (Asn38,Asn83) were identified successfully.
文摘近年来,基质辅助激光解析电离飞行时间质谱(matrix-assisted laser desorption ionization time of flight mass spectrometry,MALDI-TOF-MS)作为一种新的质谱技术成功检测出许多与疾病相关的新型生物标志物,尤其在肿瘤的诊治方面,MALDITOF-MS可以快速检测出癌症患者体内特异的蛋白表达、辅助制定患者特异性的疗法、预测患者的治疗效果以及是否有复发的可能等。近年来,国内外学者开展了多项MALDI-TOF-MS在宫颈癌、子宫内膜癌、卵巢癌三大女性生殖道恶性肿瘤中的早期筛查诊断研究,其应用于宫颈癌的蛋白组学诊断已被证实具有实验可行性;其可以检测固态、液态等多种形态样本的特点,为寻求无创早期诊断的子宫内膜癌研究者提供了新的研究方法;其在卵巢癌差异蛋白检测、化疗耐药相关分子检测中发挥了重要作用。但仍存在如何提高MALDI-TOF-MS检测的灵敏度以及满足蛋白质组学研究高通量的需要等问题。
