Aphid is a group of small insects in the Homoptera.There are approximately 4 000 aphid species all over the world.Many of them attack important agricultural crops and therefore have economic importance.However,some sp...Aphid is a group of small insects in the Homoptera.There are approximately 4 000 aphid species all over the world.Many of them attack important agricultural crops and therefore have economic importance.However,some species such as Chinese gallnut aphids are beneficial for human beings.Most aphids display complex life cycles with alternation of sexual and asexual generations and host plant alternation.Molecular genetic markers have good stabilities,high variability and mostly are free from environmental influences.So they were very suitable for aphid research and could tackle some problems which the traditional taxonomical methods could not solve.The paper introduced a simple and effective method that could extract genomic DNA from a single aphid individual and could be used for the research of molecular genetic marker such as RAPD,RFLP and DNA sequence determination.展开更多
以冷水性虹鳟鱼抗凝冷冻全血为材料,分别采用氯仿-醋酸钾法、RGDE（Rapid genomic DNA extraction）法、改进的RGDE法、传统酚-氯仿法、试剂盒法提取基因组DNA,琼脂糖凝胶电泳检测显示改进的RGDE法可以获得较高质量的鱼类基因组DNA。进...以冷水性虹鳟鱼抗凝冷冻全血为材料,分别采用氯仿-醋酸钾法、RGDE（Rapid genomic DNA extraction）法、改进的RGDE法、传统酚-氯仿法、试剂盒法提取基因组DNA,琼脂糖凝胶电泳检测显示改进的RGDE法可以获得较高质量的鱼类基因组DNA。进一步将改进的RGDE法提取血液基因组DNA的效果与传统酚-氯仿法提取血液、肌肉基因组DNA及试剂盒法提取血液基因组DNA的效果通过紫外分光光度计测定浓度和纯度进行比较。结果表明,改进RGDE法提取的基因组DNA平均浓度为1050ng/μL,极显著高于用试剂盒从血液中提取的DNA浓度,显著高于酚-氯仿法从血液和肌肉中提取的DNA浓度,DNA产量为1575μg/mL,A260/A280比值1.65-1.97。采用改进的RGDE法提取DNA,全过程只需2h,具有速度快、成本低、无污染、无需特殊设备的明显优点,而且随机扩增的结果稳定,电泳条带清晰。展开更多
文摘Aphid is a group of small insects in the Homoptera.There are approximately 4 000 aphid species all over the world.Many of them attack important agricultural crops and therefore have economic importance.However,some species such as Chinese gallnut aphids are beneficial for human beings.Most aphids display complex life cycles with alternation of sexual and asexual generations and host plant alternation.Molecular genetic markers have good stabilities,high variability and mostly are free from environmental influences.So they were very suitable for aphid research and could tackle some problems which the traditional taxonomical methods could not solve.The paper introduced a simple and effective method that could extract genomic DNA from a single aphid individual and could be used for the research of molecular genetic marker such as RAPD,RFLP and DNA sequence determination.
文摘以冷水性虹鳟鱼抗凝冷冻全血为材料,分别采用氯仿-醋酸钾法、RGDE（Rapid genomic DNA extraction）法、改进的RGDE法、传统酚-氯仿法、试剂盒法提取基因组DNA,琼脂糖凝胶电泳检测显示改进的RGDE法可以获得较高质量的鱼类基因组DNA。进一步将改进的RGDE法提取血液基因组DNA的效果与传统酚-氯仿法提取血液、肌肉基因组DNA及试剂盒法提取血液基因组DNA的效果通过紫外分光光度计测定浓度和纯度进行比较。结果表明,改进RGDE法提取的基因组DNA平均浓度为1050ng/μL,极显著高于用试剂盒从血液中提取的DNA浓度,显著高于酚-氯仿法从血液和肌肉中提取的DNA浓度,DNA产量为1575μg/mL,A260/A280比值1.65-1.97。采用改进的RGDE法提取DNA,全过程只需2h,具有速度快、成本低、无污染、无需特殊设备的明显优点,而且随机扩增的结果稳定,电泳条带清晰。
