Leptospirillum ferriphilum YSK was added to a native consortium of bioleaching bacteria including Acidithiobacillus caldus,A.thiooxidans,A.ferrooxidans,Sulfobacillus thermosulfidooxidans,Acidiphilium spp.,and Ferropla...Leptospirillum ferriphilum YSK was added to a native consortium of bioleaching bacteria including Acidithiobacillus caldus,A.thiooxidans,A.ferrooxidans,Sulfobacillus thermosulfidooxidans,Acidiphilium spp.,and Ferroplasma thermophilum cultured in modified 9K medium containing 0.5%(W/V)pyrite.The bioleaching efficiency markedly increased.Changes in community structure and gene expression were monitored with real-time PCR and functional gene arrays.Dynamic changes that varied in different populations in the consortium occurred after the addition of L.ferriphilum YSK,with growth of A.caldus S1,A.thiooxidans A01,Acidiphillum spp.DX1-1 promoted the growth of Ferroplasma L1,inhibited that of S.thermosulfidooxidans ST,and exerted little effect on that of A.ferrooxidans CMS.Genes encoding ADP heptose,phosphoheptose isomerase,glycosyltransferase,biotin carboxylase,and protoheme ferrolyase from L.ferriphilum,acetyl-CoA carboxylase from Acidiphillum spp.,and doxD from A.caldus were up-regulated in 0-20 h.Genes encoding lipid A disaccharide synthase LpxB,glycosyl transferase,and ADP heptose synthase from A.ferrooxidans were up-regulated in 0-8 h and then down-regulated in 8-20 h.Genes encoding ferredoxin oxidoreductase from Ferroplasma sp.were up-regulated in 0-4 h,down-regulated in 4-16 h,and again up-regulated in 16-20 h.CbbS from A.ferrooxidans was down-regulated in 0-20 h.展开更多
Biological desilication process is an effective way to remove silicate from rutile so that high purity rutile could be obtained. However, little is known about the molecular mechanism of this process. In this work, a ...Biological desilication process is an effective way to remove silicate from rutile so that high purity rutile could be obtained. However, little is known about the molecular mechanism of this process. In this work, a newly developed rutile bio-desilication reactor was applied to enrich rutile from rough rutile concentrate obtained from Nanzhao rutile mine and a comprehensive high through-put functional gene array(Geo Chip 4.0) was used to analyze the functional gene diversity, structure and metabolic potential of microbial communities in the biological desilication reactor. The results show that TiO2 grade of the rutile concentrate could increase from 78.21% to above 90% and the recovery rate could reach to 96% or more in 8-12 d. The results also show that almost all the key functional genes involved in the geochemical cycling process, totally 4324 and 4983 functional microorganism genes, are detected in the liquid and ore surface, respectively. There are totally 712 and 831 functional genes involved in nitrogen cycling for liquid and ore surface samples, respectively. The relative abundance of functional genes involved in the phosphorus and sulfur cycling is higher in the ore surface than liquid. These results indicate that nitrogen, phosphorus and sulfur cycling are also present in the desiliconization process of rutile. Acetogenesis genes are detected in the liquid and ore surface, which indicates that the desiliconizing process mainly depends on the function of acetic acid and other organic acids. Four silicon transporting genes are also detected in the sample, which proves that the bacteria have the potential to transfer silicon in the molecule level. It is shown that bio-desilication is an effective and environmental-friendly way for enrichment of rough rutile concentrate and presents an overview of functional diversity and structure of desilication microbial communities, which also provides insights into our understanding of metabolic potential in biological desilication reactor ecosystems.展开更多
基金Projects(51604308,41771300,41301274)supported by the National Natural Science Foundation of ChinaProject(2017QNCXTD_GTD)supported by the Youth Innovation Team Project of Institute of Subtropical Agriculture,Chinese Academy of Sciences+1 种基金Project(2017YFD0202000)supported by the National Key Research and Development Program of ChinaProject(2020GDASYL-20200402001)supported by the special Project of Science and Technology Development,China。
文摘Leptospirillum ferriphilum YSK was added to a native consortium of bioleaching bacteria including Acidithiobacillus caldus,A.thiooxidans,A.ferrooxidans,Sulfobacillus thermosulfidooxidans,Acidiphilium spp.,and Ferroplasma thermophilum cultured in modified 9K medium containing 0.5%(W/V)pyrite.The bioleaching efficiency markedly increased.Changes in community structure and gene expression were monitored with real-time PCR and functional gene arrays.Dynamic changes that varied in different populations in the consortium occurred after the addition of L.ferriphilum YSK,with growth of A.caldus S1,A.thiooxidans A01,Acidiphillum spp.DX1-1 promoted the growth of Ferroplasma L1,inhibited that of S.thermosulfidooxidans ST,and exerted little effect on that of A.ferrooxidans CMS.Genes encoding ADP heptose,phosphoheptose isomerase,glycosyltransferase,biotin carboxylase,and protoheme ferrolyase from L.ferriphilum,acetyl-CoA carboxylase from Acidiphillum spp.,and doxD from A.caldus were up-regulated in 0-20 h.Genes encoding lipid A disaccharide synthase LpxB,glycosyl transferase,and ADP heptose synthase from A.ferrooxidans were up-regulated in 0-8 h and then down-regulated in 8-20 h.Genes encoding ferredoxin oxidoreductase from Ferroplasma sp.were up-regulated in 0-4 h,down-regulated in 4-16 h,and again up-regulated in 16-20 h.CbbS from A.ferrooxidans was down-regulated in 0-20 h.
基金Project(2011-622-40) supported by the Mineral Exploration Foundation of Henan Province,ChinaProject(51104189) supported by the National Natural Science Foundation of ChinaProject(2013M531814) supported by the Postdoctoral Science Foundation of China
文摘Biological desilication process is an effective way to remove silicate from rutile so that high purity rutile could be obtained. However, little is known about the molecular mechanism of this process. In this work, a newly developed rutile bio-desilication reactor was applied to enrich rutile from rough rutile concentrate obtained from Nanzhao rutile mine and a comprehensive high through-put functional gene array(Geo Chip 4.0) was used to analyze the functional gene diversity, structure and metabolic potential of microbial communities in the biological desilication reactor. The results show that TiO2 grade of the rutile concentrate could increase from 78.21% to above 90% and the recovery rate could reach to 96% or more in 8-12 d. The results also show that almost all the key functional genes involved in the geochemical cycling process, totally 4324 and 4983 functional microorganism genes, are detected in the liquid and ore surface, respectively. There are totally 712 and 831 functional genes involved in nitrogen cycling for liquid and ore surface samples, respectively. The relative abundance of functional genes involved in the phosphorus and sulfur cycling is higher in the ore surface than liquid. These results indicate that nitrogen, phosphorus and sulfur cycling are also present in the desiliconization process of rutile. Acetogenesis genes are detected in the liquid and ore surface, which indicates that the desiliconizing process mainly depends on the function of acetic acid and other organic acids. Four silicon transporting genes are also detected in the sample, which proves that the bacteria have the potential to transfer silicon in the molecule level. It is shown that bio-desilication is an effective and environmental-friendly way for enrichment of rough rutile concentrate and presents an overview of functional diversity and structure of desilication microbial communities, which also provides insights into our understanding of metabolic potential in biological desilication reactor ecosystems.
