To investigate the effect of bamboo leaves on soil microorganism a microcosm experiment of soil mixture with different percentage of bamboo leaf powder was conducted.The biolog GN2 microtial plate was used to determin...To investigate the effect of bamboo leaves on soil microorganism a microcosm experiment of soil mixture with different percentage of bamboo leaf powder was conducted.The biolog GN2 microtial plate was used to determine diversity indices of microorganism with the incubation time. The results showed that significant differences in microbial activities measured by average well color density（AWCD） were found among treatments,and treatments 2（with 2% of leaf powder） was lower AWCD than（P<0.05） the other treatments in the 10th week.Control（with 0% of leaf powder） was higher AWCD than（P<0.05） the treatments with bamboo leaf powder in the 20th and the 30th week.It was found that in short-term bamboo leaf had improved the soil microbial activity,and AWCD values of treatments 1（with 1% of leaf powder）,2 and 3（with 4% of leaf powder） respectively reached the maximum during 2 and 10 weeks,2 and 5 weeks,5 and 10 weeks,which ware all bigger（P<0.05） than that of non-cultured soil.However,a sharp decline was observed from the time of week 20 to week 30 at which the AWCD values of treatments 1,2 and 3 with 192（hour’s） incubation after microbes being inoculated were decreased by 64.8%,55.7% and 60.4% respectively compared with non-cultured soil.The control was found no remarkable changes before the 20th week,and then a sharp decline（by 40.4% compared to non-cultured soil） was measured from the 20th to the 30th week.No significant changes in Shannon index（H） and Evenes index（J’） in the control were found,whereas a sharp decline（P<0.05） was observed for treatments with bamboo leaf from the 20th to the 30th week.McIntosh index（U） sharply decline（P<0.05） for leaf treatments from the 10th to the 20th week and till the end of this experiment（week 30）,while a significant decline in McIntosh index of the control was found from the 20th to the 30th week.In summary the added amount of leaves did not led significant difference in soil microbial diversity indices analyzed.The diversity indices of control are significantly greater（P<0.05） than the leaf treatments at the 30th week,which indicated that single litter input of bamboo leaf for long-term caused a reduction of microbial functional diversity.展开更多
采用不同酸碱度(p H 5.6,p H 4.0,p H 3.0)模拟酸雨、不同质量(400,500,600 g·m-2)柳杉Cryptomeria fortunei凋落物以及模拟酸雨与凋落物复合处理盆栽柳杉幼苗,研究酸雨和凋落物对根际土壤养分含量和微生物数量的影响。结果表明:...采用不同酸碱度(p H 5.6,p H 4.0,p H 3.0)模拟酸雨、不同质量(400,500,600 g·m-2)柳杉Cryptomeria fortunei凋落物以及模拟酸雨与凋落物复合处理盆栽柳杉幼苗,研究酸雨和凋落物对根际土壤养分含量和微生物数量的影响。结果表明:酸雨胁迫对土壤养分含量和微生物数量具有显著的影响,酸雨处理降低了土壤养分含量和细菌、放线菌数量,增加了真菌数量,影响程度均为p H 3.0>p H 4.0>p H 5.6。随着添加凋落物质量的增加,土壤中氮、磷和钾质量分数以及微生物数量均呈上升趋势,上升程度为600 g·m-2>500 g·m-2>400 g·m-2。不同p H值酸雨与凋落物复合处理中,p H 5.6+500 g·m-2凋落物和p H 4.0+500 g·m-2凋落物处理对土壤氮、磷和全钾质量分数影响均不显著,速效钾质量分数分别比对照增加了43.1%和24.0%(P<0.01);p H 3.0+500 g·m-2凋落物处理土壤全氮、碱解氮、全磷和全钾质量分数等分别比对照下降了14.0%,34.3%,30.0%和23.6%(P<0.01),有效磷质量分数下降了20.3%(P<0.05);p H 5.6+500 g·m-2凋落物处理细菌和放线菌数量分别比对照增加了15.3%和16.7%(P<0.05),p H 3.0+500 g·m-2凋落物处理细菌和放线菌数量分别比对照减少了20.9%和19.4%(P<0.01);p H 4.0+500 g·m-2凋落物和p H 3.0+500 g·m-2凋落物处理真菌数量比对照增加了100.91%和119.18%(P<0.01)。凋落物能够缓解酸雨对土壤养分质量分数和微生物数量的影响。展开更多
文摘To investigate the effect of bamboo leaves on soil microorganism a microcosm experiment of soil mixture with different percentage of bamboo leaf powder was conducted.The biolog GN2 microtial plate was used to determine diversity indices of microorganism with the incubation time. The results showed that significant differences in microbial activities measured by average well color density（AWCD） were found among treatments,and treatments 2（with 2% of leaf powder） was lower AWCD than（P<0.05） the other treatments in the 10th week.Control（with 0% of leaf powder） was higher AWCD than（P<0.05） the treatments with bamboo leaf powder in the 20th and the 30th week.It was found that in short-term bamboo leaf had improved the soil microbial activity,and AWCD values of treatments 1（with 1% of leaf powder）,2 and 3（with 4% of leaf powder） respectively reached the maximum during 2 and 10 weeks,2 and 5 weeks,5 and 10 weeks,which ware all bigger（P<0.05） than that of non-cultured soil.However,a sharp decline was observed from the time of week 20 to week 30 at which the AWCD values of treatments 1,2 and 3 with 192（hour’s） incubation after microbes being inoculated were decreased by 64.8%,55.7% and 60.4% respectively compared with non-cultured soil.The control was found no remarkable changes before the 20th week,and then a sharp decline（by 40.4% compared to non-cultured soil） was measured from the 20th to the 30th week.No significant changes in Shannon index（H） and Evenes index（J’） in the control were found,whereas a sharp decline（P<0.05） was observed for treatments with bamboo leaf from the 20th to the 30th week.McIntosh index（U） sharply decline（P<0.05） for leaf treatments from the 10th to the 20th week and till the end of this experiment（week 30）,while a significant decline in McIntosh index of the control was found from the 20th to the 30th week.In summary the added amount of leaves did not led significant difference in soil microbial diversity indices analyzed.The diversity indices of control are significantly greater（P<0.05） than the leaf treatments at the 30th week,which indicated that single litter input of bamboo leaf for long-term caused a reduction of microbial functional diversity.
文摘采用不同酸碱度(p H 5.6,p H 4.0,p H 3.0)模拟酸雨、不同质量(400,500,600 g·m-2)柳杉Cryptomeria fortunei凋落物以及模拟酸雨与凋落物复合处理盆栽柳杉幼苗,研究酸雨和凋落物对根际土壤养分含量和微生物数量的影响。结果表明:酸雨胁迫对土壤养分含量和微生物数量具有显著的影响,酸雨处理降低了土壤养分含量和细菌、放线菌数量,增加了真菌数量,影响程度均为p H 3.0>p H 4.0>p H 5.6。随着添加凋落物质量的增加,土壤中氮、磷和钾质量分数以及微生物数量均呈上升趋势,上升程度为600 g·m-2>500 g·m-2>400 g·m-2。不同p H值酸雨与凋落物复合处理中,p H 5.6+500 g·m-2凋落物和p H 4.0+500 g·m-2凋落物处理对土壤氮、磷和全钾质量分数影响均不显著,速效钾质量分数分别比对照增加了43.1%和24.0%(P<0.01);p H 3.0+500 g·m-2凋落物处理土壤全氮、碱解氮、全磷和全钾质量分数等分别比对照下降了14.0%,34.3%,30.0%和23.6%(P<0.01),有效磷质量分数下降了20.3%(P<0.05);p H 5.6+500 g·m-2凋落物处理细菌和放线菌数量分别比对照增加了15.3%和16.7%(P<0.05),p H 3.0+500 g·m-2凋落物处理细菌和放线菌数量分别比对照减少了20.9%和19.4%(P<0.01);p H 4.0+500 g·m-2凋落物和p H 3.0+500 g·m-2凋落物处理真菌数量比对照增加了100.91%和119.18%(P<0.01)。凋落物能够缓解酸雨对土壤养分质量分数和微生物数量的影响。
