分离筛选高效好氧反硝化聚磷细菌,探究氮代谢途径,优化最佳脱氮除磷条件,为废水生物脱氮除磷提供菌种资源及技术参考。基于BTB鉴别培养基以稀释涂布法从污水处理厂活性污泥中分离筛选高效反硝化聚磷细菌,结合形态观察和16S r RNA基因系...分离筛选高效好氧反硝化聚磷细菌,探究氮代谢途径,优化最佳脱氮除磷条件,为废水生物脱氮除磷提供菌种资源及技术参考。基于BTB鉴别培养基以稀释涂布法从污水处理厂活性污泥中分离筛选高效反硝化聚磷细菌,结合形态观察和16S r RNA基因系统发育分析完成种属鉴定;通过中间代谢产物检测和氮平衡分析探究脱氮途径;单因素实验法优化菌株的脱氮除磷条件。分离筛选到高效反硝化聚磷细菌D8,鉴定为气单胞菌(Aeromonas sp.),在富氮富磷培养基中24 h时PO_(4)^(3-)-P、NO_(3)^(-)-N和NH_(4)^(+)-N的去除率分别为100%、92.24%和87.20%;菌株D8具有氨同化、硝酸盐还原和异养硝化-好氧反硝化三种复合氮代谢途径;最佳脱氮除磷条件为Na Ac为碳源、C/N为9、p H 7.0~9.0、28℃。展开更多
In order to further explore the mechanism of denitrifying phosphorus removal,the growth characteristics and denitrifying phosphorus removal characteristics of a typical denitrifying phosphate accumulating bacteria(DPB...In order to further explore the mechanism of denitrifying phosphorus removal,the growth characteristics and denitrifying phosphorus removal characteristics of a typical denitrifying phosphate accumulating bacteria(DPB) which was isolated by previous experiment were studied.The effect of different electron acceptors on the efficiency of nitrogen and phosphorus removal was investigated by pure bacteria and reactor statics simulation tests.The growth curve of the DPB was typical,and its incubation period was less than 1 h and the logarithmic phase about 14 h.The result showed that the stain could utilize both nitrite and nitrate as different electron acceptors to take up phosphorus.And there was a good linear correlation between P removal and NO-3-N removal during phosphorus removal process.The sensitivity to nitrite of the strain was low.The effect of electron acceptors on phosphorus removal was not significant.The efficiency of removal for phosphorus and the nitrate was both above 60%,and that for nitrite was 100%.展开更多
旨在从环境样品中筛选对富营养化水体具有良好脱氮除磷效果的好氧反硝化菌。采集福州某养猪场污水处理池中的水样。通过反硝化细菌培养基培养、BTB培养基平板分离、硝酸盐还原试验和蓝白斑筛选法、异染颗粒以及聚-β-羟基丁酸(PHB)颗粒...旨在从环境样品中筛选对富营养化水体具有良好脱氮除磷效果的好氧反硝化菌。采集福州某养猪场污水处理池中的水样。通过反硝化细菌培养基培养、BTB培养基平板分离、硝酸盐还原试验和蓝白斑筛选法、异染颗粒以及聚-β-羟基丁酸(PHB)颗粒染色试验,筛选获得两株具有脱氮除磷特性的菌株,命名为N1和N2。经16S r RNA基因序列分析,N1和N2分别属于无色杆菌属(Achromobacter.sp)和短波单胞菌属(Brevundimonas.sp)。将菌株N1和N2复配,获得脱氮除磷复合菌FIM-1。考察了菌株对人工合成污水和富营养化水体脱氮除磷的效果。结果表明,两株菌在含磷量较低的水体中,对磷的去除率较高,相对于单菌,复合菌表现出更佳的脱氮除磷效果。展开更多
文摘分离筛选高效好氧反硝化聚磷细菌,探究氮代谢途径,优化最佳脱氮除磷条件,为废水生物脱氮除磷提供菌种资源及技术参考。基于BTB鉴别培养基以稀释涂布法从污水处理厂活性污泥中分离筛选高效反硝化聚磷细菌,结合形态观察和16S r RNA基因系统发育分析完成种属鉴定;通过中间代谢产物检测和氮平衡分析探究脱氮途径;单因素实验法优化菌株的脱氮除磷条件。分离筛选到高效反硝化聚磷细菌D8,鉴定为气单胞菌(Aeromonas sp.),在富氮富磷培养基中24 h时PO_(4)^(3-)-P、NO_(3)^(-)-N和NH_(4)^(+)-N的去除率分别为100%、92.24%和87.20%;菌株D8具有氨同化、硝酸盐还原和异养硝化-好氧反硝化三种复合氮代谢途径;最佳脱氮除磷条件为Na Ac为碳源、C/N为9、p H 7.0~9.0、28℃。
文摘In order to further explore the mechanism of denitrifying phosphorus removal,the growth characteristics and denitrifying phosphorus removal characteristics of a typical denitrifying phosphate accumulating bacteria(DPB) which was isolated by previous experiment were studied.The effect of different electron acceptors on the efficiency of nitrogen and phosphorus removal was investigated by pure bacteria and reactor statics simulation tests.The growth curve of the DPB was typical,and its incubation period was less than 1 h and the logarithmic phase about 14 h.The result showed that the stain could utilize both nitrite and nitrate as different electron acceptors to take up phosphorus.And there was a good linear correlation between P removal and NO-3-N removal during phosphorus removal process.The sensitivity to nitrite of the strain was low.The effect of electron acceptors on phosphorus removal was not significant.The efficiency of removal for phosphorus and the nitrate was both above 60%,and that for nitrite was 100%.
文摘旨在从环境样品中筛选对富营养化水体具有良好脱氮除磷效果的好氧反硝化菌。采集福州某养猪场污水处理池中的水样。通过反硝化细菌培养基培养、BTB培养基平板分离、硝酸盐还原试验和蓝白斑筛选法、异染颗粒以及聚-β-羟基丁酸(PHB)颗粒染色试验,筛选获得两株具有脱氮除磷特性的菌株,命名为N1和N2。经16S r RNA基因序列分析,N1和N2分别属于无色杆菌属(Achromobacter.sp)和短波单胞菌属(Brevundimonas.sp)。将菌株N1和N2复配,获得脱氮除磷复合菌FIM-1。考察了菌株对人工合成污水和富营养化水体脱氮除磷的效果。结果表明,两株菌在含磷量较低的水体中,对磷的去除率较高,相对于单菌,复合菌表现出更佳的脱氮除磷效果。
