分离筛选高效好氧反硝化聚磷细菌,探究氮代谢途径,优化最佳脱氮除磷条件,为废水生物脱氮除磷提供菌种资源及技术参考。基于BTB鉴别培养基以稀释涂布法从污水处理厂活性污泥中分离筛选高效反硝化聚磷细菌,结合形态观察和16S r RNA基因系...分离筛选高效好氧反硝化聚磷细菌,探究氮代谢途径,优化最佳脱氮除磷条件,为废水生物脱氮除磷提供菌种资源及技术参考。基于BTB鉴别培养基以稀释涂布法从污水处理厂活性污泥中分离筛选高效反硝化聚磷细菌,结合形态观察和16S r RNA基因系统发育分析完成种属鉴定;通过中间代谢产物检测和氮平衡分析探究脱氮途径;单因素实验法优化菌株的脱氮除磷条件。分离筛选到高效反硝化聚磷细菌D8,鉴定为气单胞菌(Aeromonas sp.),在富氮富磷培养基中24 h时PO_(4)^(3-)-P、NO_(3)^(-)-N和NH_(4)^(+)-N的去除率分别为100%、92.24%和87.20%;菌株D8具有氨同化、硝酸盐还原和异养硝化-好氧反硝化三种复合氮代谢途径;最佳脱氮除磷条件为Na Ac为碳源、C/N为9、p H 7.0~9.0、28℃。展开更多
In order to further explore the mechanism of denitrifying phosphorus removal,the growth characteristics and denitrifying phosphorus removal characteristics of a typical denitrifying phosphate accumulating bacteria(DPB...In order to further explore the mechanism of denitrifying phosphorus removal,the growth characteristics and denitrifying phosphorus removal characteristics of a typical denitrifying phosphate accumulating bacteria(DPB) which was isolated by previous experiment were studied.The effect of different electron acceptors on the efficiency of nitrogen and phosphorus removal was investigated by pure bacteria and reactor statics simulation tests.The growth curve of the DPB was typical,and its incubation period was less than 1 h and the logarithmic phase about 14 h.The result showed that the stain could utilize both nitrite and nitrate as different electron acceptors to take up phosphorus.And there was a good linear correlation between P removal and NO-3-N removal during phosphorus removal process.The sensitivity to nitrite of the strain was low.The effect of electron acceptors on phosphorus removal was not significant.The efficiency of removal for phosphorus and the nitrate was both above 60%,and that for nitrite was 100%.展开更多
文摘分离筛选高效好氧反硝化聚磷细菌,探究氮代谢途径,优化最佳脱氮除磷条件,为废水生物脱氮除磷提供菌种资源及技术参考。基于BTB鉴别培养基以稀释涂布法从污水处理厂活性污泥中分离筛选高效反硝化聚磷细菌,结合形态观察和16S r RNA基因系统发育分析完成种属鉴定;通过中间代谢产物检测和氮平衡分析探究脱氮途径;单因素实验法优化菌株的脱氮除磷条件。分离筛选到高效反硝化聚磷细菌D8,鉴定为气单胞菌(Aeromonas sp.),在富氮富磷培养基中24 h时PO_(4)^(3-)-P、NO_(3)^(-)-N和NH_(4)^(+)-N的去除率分别为100%、92.24%和87.20%;菌株D8具有氨同化、硝酸盐还原和异养硝化-好氧反硝化三种复合氮代谢途径;最佳脱氮除磷条件为Na Ac为碳源、C/N为9、p H 7.0~9.0、28℃。
文摘In order to further explore the mechanism of denitrifying phosphorus removal,the growth characteristics and denitrifying phosphorus removal characteristics of a typical denitrifying phosphate accumulating bacteria(DPB) which was isolated by previous experiment were studied.The effect of different electron acceptors on the efficiency of nitrogen and phosphorus removal was investigated by pure bacteria and reactor statics simulation tests.The growth curve of the DPB was typical,and its incubation period was less than 1 h and the logarithmic phase about 14 h.The result showed that the stain could utilize both nitrite and nitrate as different electron acceptors to take up phosphorus.And there was a good linear correlation between P removal and NO-3-N removal during phosphorus removal process.The sensitivity to nitrite of the strain was low.The effect of electron acceptors on phosphorus removal was not significant.The efficiency of removal for phosphorus and the nitrate was both above 60%,and that for nitrite was 100%.
