A reversed capillary electrophoresis with contactless high frequency conductivity detection method to determine amino acids mixture was described. In this study,cetyltrimethylammonium bromide（CTAB） was used to rever...A reversed capillary electrophoresis with contactless high frequency conductivity detection method to determine amino acids mixture was described. In this study,cetyltrimethylammonium bromide（CTAB） was used to reverse electro-osmotic flow in order to shorten the analysis time. β-CD was added to optimize the separation further. The effects of separation potential,concentration of CTAB,buffer electrolyte and β-CD etc. were researched and the best analysis conditions were obtained. Ten kinds of amino acids were separated and detected directly in running medium of 15.0 mmol/L diethylamine/0.10 mmol/L H3BO3/0.10 mmol/L CTAB/0.25 mmol/L β-CD（pH=11.0） at the separation potential of -20 kV within 7 min. The correlation coefficients of amino acids are 0.991—0.999 in the linear range of 1.00×10 -6 —1.00×10 -3 mol/L,the limit of detection are 0.17—0.94 μmol/L（S/N=3）. [WT5HZ]展开更多
文摘A reversed capillary electrophoresis with contactless high frequency conductivity detection method to determine amino acids mixture was described. In this study,cetyltrimethylammonium bromide（CTAB） was used to reverse electro-osmotic flow in order to shorten the analysis time. β-CD was added to optimize the separation further. The effects of separation potential,concentration of CTAB,buffer electrolyte and β-CD etc. were researched and the best analysis conditions were obtained. Ten kinds of amino acids were separated and detected directly in running medium of 15.0 mmol/L diethylamine/0.10 mmol/L H3BO3/0.10 mmol/L CTAB/0.25 mmol/L β-CD（pH=11.0） at the separation potential of -20 kV within 7 min. The correlation coefficients of amino acids are 0.991—0.999 in the linear range of 1.00×10 -6 —1.00×10 -3 mol/L,the limit of detection are 0.17—0.94 μmol/L（S/N=3）. [WT5HZ]
