为了促进厌氧氨氧化菌(AnAOB)生长,实现厌氧氨氧化(Anammox)污泥的快速富集,以处理养殖沼液的自然塘污泥为种泥,定期加载超声进行强化富集Anammox污泥(R2反应器),同时设置未加载超声的对照组(R1反应器),考察污泥长期驯化培养过程中反应...为了促进厌氧氨氧化菌(AnAOB)生长,实现厌氧氨氧化(Anammox)污泥的快速富集,以处理养殖沼液的自然塘污泥为种泥,定期加载超声进行强化富集Anammox污泥(R2反应器),同时设置未加载超声的对照组(R1反应器),考察污泥长期驯化培养过程中反应器的脱氮性能和微生物群落结构的变化情况。结果表明:(1)在180 W 5 min超声2次强化下,56 d可成功实现Anammox污泥的富集培养,其氨氮和亚硝酸盐氮的去除速率分别为228.09、273.88 mg/(L·d);(2)加载超声后,污泥胞外聚合物(EPS)的分泌量不断增加,其中蛋白质(PN)的分泌量大于多糖(PS),PN/PS也在逐步升高;(3)经扫描电子显微镜、高通量测序、荧光原位杂交综合分析后发现,加载超声的R2反应器中AnAOB相对丰度逐步增加;未加载超声的R1反应器中AnAOB相对丰度先增加后减少,而反硝化菌相对丰度则大幅增加。由此可见,加载180 W 5 min超声有利于选择性富集培养Anammox污泥。展开更多
采用厌氧反应器接种厌氧氨氧化污泥,研究了脱氮效能提高过程中颗粒污泥的沉淀性能及不同策略对增强颗粒污泥沉淀性的影响。结果表明:当反应器氮去除速率达到3.2 kg m-3 d-1时,产气量的增加使得颗粒污泥出现大量流失。采用"粉碎-回...采用厌氧反应器接种厌氧氨氧化污泥,研究了脱氮效能提高过程中颗粒污泥的沉淀性能及不同策略对增强颗粒污泥沉淀性的影响。结果表明:当反应器氮去除速率达到3.2 kg m-3 d-1时,产气量的增加使得颗粒污泥出现大量流失。采用"粉碎-回流-重新形成颗粒污泥"方式不能完全有效地改善颗粒污泥的沉降性能。通过对反应器内和流失的污泥进行测定发现,相同粒径的反应器内颗粒污泥的MLVSS/MLSS值要明显低于流失的颗粒污泥。通过CaSO4颗粒投加,回流的流失污泥会以CaSO4作为颗粒污泥的核。经过48 d的培养,污泥的沉降性能得到良好的改善,反应器氮去除速率从5.23 kg m-3 d-1上升到9.2 kg m-3 d-1。同时该研究还发现当进水总氮浓度达到1320 mg L-1时,厌氧氨氧化颗粒污泥活性并未受到影响,说明厌氧氨氧化颗粒污泥对进水基质浓度具有较强的耐受性。展开更多
In this study, a lab-scale upflow anaerobic sludge blanket(UASB) reactor was applied to studying the high-rate nitrogen removal of granule-based anammox process. The nitrogen removal rate(NRR) finally improved to 15.7...In this study, a lab-scale upflow anaerobic sludge blanket(UASB) reactor was applied to studying the high-rate nitrogen removal of granule-based anammox process. The nitrogen removal rate(NRR) finally improved to 15.77 kg/m3/d by shortening hydraulic retention time(HRT) to 1.06 h. Well-shaped red anammox granules were extensively enriched inside the reactor. The results of nitrogen removal kinetics indicated that the present bioreactor has great nitrogen removal potential, because the maximum rate of substrate utilization(Umax) predicted by Stover-Kincannon model is suggested as 55.68 kg/(m3·d). Analysis of the microbial community showed that the anammox genus Candidatus Kuenenia dominated the bacterial communities. The relative abundance of Candidatus Kuenenia rose from 12.29% to 36.95% after progressively shorter HRT and higher influent substrate concentrations, illustrating the stability of nitrogen removal performance and biomass enrichment offered by the UASB in carrying out high-rate anammox process.展开更多
文摘为了促进厌氧氨氧化菌(AnAOB)生长,实现厌氧氨氧化(Anammox)污泥的快速富集,以处理养殖沼液的自然塘污泥为种泥,定期加载超声进行强化富集Anammox污泥(R2反应器),同时设置未加载超声的对照组(R1反应器),考察污泥长期驯化培养过程中反应器的脱氮性能和微生物群落结构的变化情况。结果表明:(1)在180 W 5 min超声2次强化下,56 d可成功实现Anammox污泥的富集培养,其氨氮和亚硝酸盐氮的去除速率分别为228.09、273.88 mg/(L·d);(2)加载超声后,污泥胞外聚合物(EPS)的分泌量不断增加,其中蛋白质(PN)的分泌量大于多糖(PS),PN/PS也在逐步升高;(3)经扫描电子显微镜、高通量测序、荧光原位杂交综合分析后发现,加载超声的R2反应器中AnAOB相对丰度逐步增加;未加载超声的R1反应器中AnAOB相对丰度先增加后减少,而反硝化菌相对丰度则大幅增加。由此可见,加载180 W 5 min超声有利于选择性富集培养Anammox污泥。
文摘采用厌氧反应器接种厌氧氨氧化污泥,研究了脱氮效能提高过程中颗粒污泥的沉淀性能及不同策略对增强颗粒污泥沉淀性的影响。结果表明:当反应器氮去除速率达到3.2 kg m-3 d-1时,产气量的增加使得颗粒污泥出现大量流失。采用"粉碎-回流-重新形成颗粒污泥"方式不能完全有效地改善颗粒污泥的沉降性能。通过对反应器内和流失的污泥进行测定发现,相同粒径的反应器内颗粒污泥的MLVSS/MLSS值要明显低于流失的颗粒污泥。通过CaSO4颗粒投加,回流的流失污泥会以CaSO4作为颗粒污泥的核。经过48 d的培养,污泥的沉降性能得到良好的改善,反应器氮去除速率从5.23 kg m-3 d-1上升到9.2 kg m-3 d-1。同时该研究还发现当进水总氮浓度达到1320 mg L-1时,厌氧氨氧化颗粒污泥活性并未受到影响,说明厌氧氨氧化颗粒污泥对进水基质浓度具有较强的耐受性。
基金Project(51878662)supported by the National Natural Science Foundation of ChinaProject(2017SK2420)supported by the Science and Technology of Hunan Province,ChinaProject(2019JJ20033)supported by the Distinguished Youth Natural Science Foundation of Hunan Province,China。
文摘In this study, a lab-scale upflow anaerobic sludge blanket(UASB) reactor was applied to studying the high-rate nitrogen removal of granule-based anammox process. The nitrogen removal rate(NRR) finally improved to 15.77 kg/m3/d by shortening hydraulic retention time(HRT) to 1.06 h. Well-shaped red anammox granules were extensively enriched inside the reactor. The results of nitrogen removal kinetics indicated that the present bioreactor has great nitrogen removal potential, because the maximum rate of substrate utilization(Umax) predicted by Stover-Kincannon model is suggested as 55.68 kg/(m3·d). Analysis of the microbial community showed that the anammox genus Candidatus Kuenenia dominated the bacterial communities. The relative abundance of Candidatus Kuenenia rose from 12.29% to 36.95% after progressively shorter HRT and higher influent substrate concentrations, illustrating the stability of nitrogen removal performance and biomass enrichment offered by the UASB in carrying out high-rate anammox process.
