An individual cell is a chemical reactor with thousands of reactions operated under a highly sophisticated control system,and it is a self-regulated reactor that can alter its biosynthetic machinery to meet its demand...An individual cell is a chemical reactor with thousands of reactions operated under a highly sophisticated control system,and it is a self-regulated reactor that can alter its biosynthetic machinery to meet its demands in a changing environment.A simple mathematical model for the growth of a single cell of hybridoma is presented in this work in which the cell is divided into structural and genetic compartments.This model can describe the changes in cell component,cell shape,cell size,substrate uptake, byproduct release and other temporal events during the cell division cycle.Some model parameters were regressed with experimental gross data of hybridoma in continuous culture,and specific uptake rate of glucose and glutamine of hybridoma in continuous culture were predicted.The predicted values were in good agreement with experimental data.The average relative deviation is 13.8%.展开更多
文摘An individual cell is a chemical reactor with thousands of reactions operated under a highly sophisticated control system,and it is a self-regulated reactor that can alter its biosynthetic machinery to meet its demands in a changing environment.A simple mathematical model for the growth of a single cell of hybridoma is presented in this work in which the cell is divided into structural and genetic compartments.This model can describe the changes in cell component,cell shape,cell size,substrate uptake, byproduct release and other temporal events during the cell division cycle.Some model parameters were regressed with experimental gross data of hybridoma in continuous culture,and specific uptake rate of glucose and glutamine of hybridoma in continuous culture were predicted.The predicted values were in good agreement with experimental data.The average relative deviation is 13.8%.
