【目的】建立并优化布鲁氏菌天然半抗原琼脂扩散试验(Native hapten agar gel immuno-diffusion test,NH-AGID),检测临床样品。【方法】建立NH-AGID方法,评价方法特异性、敏感性及重复性;检测免疫地区2287份、非免疫地区252份牛、羊血...【目的】建立并优化布鲁氏菌天然半抗原琼脂扩散试验(Native hapten agar gel immuno-diffusion test,NH-AGID),检测临床样品。【方法】建立NH-AGID方法,评价方法特异性、敏感性及重复性;检测免疫地区2287份、非免疫地区252份牛、羊血清。【结果】建立了NH-AGID方法,检测稳定,重复性好,具有较高的特异性;对自然感染阳性动物检测敏感性低于80%,对排菌动物的检测敏感性高于90%,检出布病阳性抗体的阈值高于RBT。免疫地区血清NH抗体平均检出率17.8%,LPS抗体平均检出率52.3%;非免疫地区血清NH抗体平均检出率40.9%,LPS抗体平均检出率54.8%。【结论】应用NH-AGID方法可鉴别区分免疫地区牛、羊布病感染动物和免疫动物。检出NH抗体动物处于布鲁氏菌活动期或排菌期为布病感染动物,应及时剔除出群。展开更多
rac-Tetra（m-nitrobenzene）porphyrin, H2TNO2PP, synthesized from o-nitro-benzaldehyde and pyrrole was reduced to H2TamPP, from which tetra（o-phenylacetyl benzene） porphyrin and especially its α,α,α,α-isomer were...rac-Tetra（m-nitrobenzene）porphyrin, H2TNO2PP, synthesized from o-nitro-benzaldehyde and pyrrole was reduced to H2TamPP, from which tetra（o-phenylacetyl benzene） porphyrin and especially its α,α,α,α-isomer were obtained. The α,α,α,α-isomer was used as hapten to immunize BALB/C mice. The latter gave antibody value of 320 as determined by ELISA method, indicating that the artificial hapten could induce the production of antibody without use of carrier.展开更多
The thiol group of glutathione (GSH) was protected by 2, 4-dinitrochlorobenzene(DNCB). The product GSH-S-DNP was reacted with 2-methyl-1-propanol and 3-methyl-1-butanol respectively to obtain hapten6 and 7. Their stru...The thiol group of glutathione (GSH) was protected by 2, 4-dinitrochlorobenzene(DNCB). The product GSH-S-DNP was reacted with 2-methyl-1-propanol and 3-methyl-1-butanol respectively to obtain hapten6 and 7. Their structures were determined by 400 MHz1H NMR and IR. The two haptens were then covalently crosslinked with bovine serum albu-min(BSA) hy using glutaraldehyde as coupling agent to obtain antigen 6 and 7. The resultsof gel electrophoresis analysis gave the average molecular weight of 1. 27 ×10-22 kg for eachantigen. The average connected ratio of BSA to hapten was 1: 12. Other UV and fluores-cence spectral properties of the antigens are reported.展开更多
文摘【目的】建立并优化布鲁氏菌天然半抗原琼脂扩散试验(Native hapten agar gel immuno-diffusion test,NH-AGID),检测临床样品。【方法】建立NH-AGID方法,评价方法特异性、敏感性及重复性;检测免疫地区2287份、非免疫地区252份牛、羊血清。【结果】建立了NH-AGID方法,检测稳定,重复性好,具有较高的特异性;对自然感染阳性动物检测敏感性低于80%,对排菌动物的检测敏感性高于90%,检出布病阳性抗体的阈值高于RBT。免疫地区血清NH抗体平均检出率17.8%,LPS抗体平均检出率52.3%;非免疫地区血清NH抗体平均检出率40.9%,LPS抗体平均检出率54.8%。【结论】应用NH-AGID方法可鉴别区分免疫地区牛、羊布病感染动物和免疫动物。检出NH抗体动物处于布鲁氏菌活动期或排菌期为布病感染动物,应及时剔除出群。
文摘rac-Tetra（m-nitrobenzene）porphyrin, H2TNO2PP, synthesized from o-nitro-benzaldehyde and pyrrole was reduced to H2TamPP, from which tetra（o-phenylacetyl benzene） porphyrin and especially its α,α,α,α-isomer were obtained. The α,α,α,α-isomer was used as hapten to immunize BALB/C mice. The latter gave antibody value of 320 as determined by ELISA method, indicating that the artificial hapten could induce the production of antibody without use of carrier.
文摘The thiol group of glutathione (GSH) was protected by 2, 4-dinitrochlorobenzene(DNCB). The product GSH-S-DNP was reacted with 2-methyl-1-propanol and 3-methyl-1-butanol respectively to obtain hapten6 and 7. Their structures were determined by 400 MHz1H NMR and IR. The two haptens were then covalently crosslinked with bovine serum albu-min(BSA) hy using glutaraldehyde as coupling agent to obtain antigen 6 and 7. The resultsof gel electrophoresis analysis gave the average molecular weight of 1. 27 ×10-22 kg for eachantigen. The average connected ratio of BSA to hapten was 1: 12. Other UV and fluores-cence spectral properties of the antigens are reported.
