A total of 144 F2 individuals were obtained from the crossing between 1047 (susceptible) and A21 (resistant). Two RAPD markers were screened out in 200 random primers using BSA(Bulked Segregant Analysis). Two RAPD mar...A total of 144 F2 individuals were obtained from the crossing between 1047 (susceptible) and A21 (resistant). Two RAPD markers were screened out in 200 random primers using BSA(Bulked Segregant Analysis). Two RAPD markers, designated as AG13/2000 and U16/660, were 7.7 cM and 8.38 cM apart from the TuMV resistant gene, respectively. The two RAPD fragments were converted to SCAR markers. SCAR markers were confined in germplasm.展开更多
文摘A total of 144 F2 individuals were obtained from the crossing between 1047 (susceptible) and A21 (resistant). Two RAPD markers were screened out in 200 random primers using BSA(Bulked Segregant Analysis). Two RAPD markers, designated as AG13/2000 and U16/660, were 7.7 cM and 8.38 cM apart from the TuMV resistant gene, respectively. The two RAPD fragments were converted to SCAR markers. SCAR markers were confined in germplasm.
