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鸡蛋中氟苯尼考残留的AlphaLISA检测方法的建立
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作者 孙志文 王忠 +5 位作者 于莹 赵营 张晶晶 魏紫嫣 方芳 马立才 《中国兽医杂志》 北大核心 2025年第4期11-19,共9页
本试验旨在建立可检测鸡蛋中氟苯尼考(FF)残留的均相光激化学发光免疫分析方法(AlphaLISA)。通过化学方法合成FF生物素半抗原,利用O-羧甲基羟胺法偶联FF抗体与受体微球,通过筛选确定鸡蛋样品稀释倍数,采用棋盘法确定FF抗体标记受体微球... 本试验旨在建立可检测鸡蛋中氟苯尼考(FF)残留的均相光激化学发光免疫分析方法(AlphaLISA)。通过化学方法合成FF生物素半抗原,利用O-羧甲基羟胺法偶联FF抗体与受体微球,通过筛选确定鸡蛋样品稀释倍数,采用棋盘法确定FF抗体标记受体微球和FF生物素半抗原的浓度,最后基于FF抗体标记受体微球、FF生物素半抗原和链霉亲和素修饰供体微球,优化建立了检测鸡蛋中FF残留的AlphaLISA检测方法,并评价其灵敏度、特异性、准确性和重复性,及与国家标准方法GB 31658.20—2022《动物性食品中酰胺醇类药物及其代谢物残留量的测定液相色谱-串联质谱法》的一致性。质谱分析结果显示,FF生物素半抗原与目标分子的分子量一致(636 Da)。经评估,鸡蛋样品离心上清液采用25倍稀释方案。建立的AlphaLISA方法对鸡蛋中FF的检测限为0.57μg/kg,定量限为0.92μg/kg;添标回收率介于87.00%~99.50%,日内和日间变异系数均低于5%;与氟苯尼考胺的交叉反应率较高(93.27%),与12种常见兽药的交叉反应率极低(<0.1%);与国家标准方法比较,2种方法在5个添加浓度点的检测结果高度一致(R2=0.997)。结果表明,本试验建立的AlphaLISA具有高灵敏度、准确性、重复性和操作简便等优点,适用于鸡蛋中FF残留的快速检测,为鸡蛋安全监测提供了高效且可行的方案。 展开更多
关键词 鸡蛋 氟苯尼考 均相光激化发光免疫分析方法(AlphaLISA) 半抗原
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急性髓系白血病患者预后不良相关基因的多组学分析 被引量:16
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作者 陈熙勐 张皓旻 +2 位作者 杨波 卢学春 贺培凤 《中国实验血液学杂志》 CAS CSCD 北大核心 2019年第2期331-338,共8页
目的:利用GEO数据库和TCGA数据库的数据,通过多组学分析方法分析与急性髓系白血病(acute myeloid leukemia,AML)发生、发展及不良预后相关的分子标志物。方法:从GEO数据库下载符合要求的转录组数据,运用R语言Limma程序包进行差异表达基... 目的:利用GEO数据库和TCGA数据库的数据,通过多组学分析方法分析与急性髓系白血病(acute myeloid leukemia,AML)发生、发展及不良预后相关的分子标志物。方法:从GEO数据库下载符合要求的转录组数据,运用R语言Limma程序包进行差异表达基因的筛选,并对差异表达基因进行GO功能富集分析和KEGG通路富集分析,同时使用STRING数据库数据,利用Cytoscape软件构建蛋白质相互作用网络,筛选出hub gene,结合TCGA数据库附带的临床信息对hub gene进行预后分析。结果:共筛选出620个差异基因,上调的差异表达基因162个,下调的差异表达基因458个。综合GO功能富集、KEGG通路富集分析及蛋白相互作用网络结果,筛选出CXCL4、CXCR4、CXCR1、CXCR2、CCL5、JUN为hub gene。生存分析显示,CXCL4、CXCR1、CCL5高表达是患者预后不良的危险因素。结论:CXCL4、CXCR1、CCL5可以作为AML发生、发展的相关生物标志物,且与不良预后相关,这可以为进一步研究提供依据。 展开更多
关键词 急性髓系白血病 多组分析方法 生物信息
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食品安全与药物代谢分析研究评述
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作者 孙汉文 《河北大学学报(自然科学版)》 CAS 北大核心 2021年第5期545-550,共6页
扼要介绍现代分析化学的发展,综述作者指导研究生近20年来在分离科学研究、食品安全分析、药物代谢分析等领域的研究进展,期望进一步开展食品组学和药物代谢组学分析研究.
关键词 综述 分析方法学 食品安全分析 药物代谢分析研究
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Stability analysis of three-dimensional two-bench unsaturated soil slopes under seismic loading
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作者 SHAN Jun-tao WU Yi-min YANG Xiao-li 《Journal of Central South University》 CSCD 2024年第11期4187-4202,共16页
Due to rainfall infiltration,groundwater activity,geological processes,and natural erosion,soil often exhibits heterogeneity and unsaturation.Additionally,seismic events can compromise slope stability.Existing analyti... Due to rainfall infiltration,groundwater activity,geological processes,and natural erosion,soil often exhibits heterogeneity and unsaturation.Additionally,seismic events can compromise slope stability.Existing analytical solutions typically consider a single failure mode,leading to inaccurate slope stability assessments.This study analyzes the impact of matric suction through three nonlinear shear strength models and adopts a heterogeneous soil model where cohesion linearly increases with depth.An improved pseudo-dynamic method is used to account for seismic effects.Based on a three-dimensional(3D)trumpet-shaped rotational failure mechanism,a new framework is established to analyze the stability of 3D two-bench slopes in heterogeneous unsaturated soil under seismic effects.The internal energy dissipation rate and external power at failure are calculated,and the gravity increase method is introduced to derive an explicit expression for the safety factor(F_(s)).The results are compared with previously published results,demonstrating the effectiveness of the proposed method.Sensitivity analyses on different parameters are conducted,discussing the influence of various factors on F s.This study proposes a new formula for calculating the F_(s) of 3D two-bench slopes in heterogeneous unsaturated soil under seismic effects,providing a practical application for slope engineering. 展开更多
关键词 3D two-bench slope limit analysis modified pseudo-dynamic method HETEROGENEITY UNSATURATION
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基于生态系统服务价值评价的忻州市生态安全格局构建 被引量:10
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作者 张纹嘉 付勇勇 王晓军 《水土保持研究》 CSCD 北大核心 2023年第4期355-364,共10页
[目的]准确识别生态保护的重要节点和区域,进而合理布局及建设生态廊道,维护生态系统可持续发展。[方法]以忻州市为例,基于生态源地-阻力面-生态网络的生态安全格局构建方法,通过生态系统服务价值评价及形态学空间学格局分析方法(MSPA)... [目的]准确识别生态保护的重要节点和区域,进而合理布局及建设生态廊道,维护生态系统可持续发展。[方法]以忻州市为例,基于生态源地-阻力面-生态网络的生态安全格局构建方法,通过生态系统服务价值评价及形态学空间学格局分析方法(MSPA)识别生态源地;基于熵权法和多阻力因子构建阻力面;运用电路理论模拟分析生态廊道和生态节点,确定生态修复的一些关键区域。[结果](1)研究区内含有生态源地59处,总面积为2355 km^(2),呈现出东部集中,中西部分散的空间分布格局。(2)研究区共有生态廊道100条左右(总长度为1637.58 km),生态夹点111处(总面积为3.16 km^(2))以及生态障碍点83处(总面积为48.7 km^(2)),生态廊道按其重要程度呈现自内向外的环形分布,而生态夹点则主要散布在生态源区和廊道相互毗邻的区域。[结论]生态安全格局能够有效识别生态廊道以及生态修复的重要区域。在生态保护与恢复中,应将“保护廊道、修复夹点区域、剔除和完善障碍区域”作为总体治理策略。 展开更多
关键词 生态安全格局 生态系统服务价值 形态空间分析方法 最小累计阻力模型
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A new method determining safe thickness of karst cave roof under pile tip 被引量:21
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作者 蒋冲 刘浪 吴均平 《Journal of Central South University》 SCIE EI CAS 2014年第3期1190-1196,共7页
Cave roofs are used to support pile foundation in many engineering projects. Accurate stability analysis method of cave roof under pile tip is important in order to ensure the safety of the pile foundation structure. ... Cave roofs are used to support pile foundation in many engineering projects. Accurate stability analysis method of cave roof under pile tip is important in order to ensure the safety of the pile foundation structure. Firstly the mechanical model to analysis the stability of cave roof under pile tip is founded aiming to solve the problems that the simplified mechanical model has. Secondly, the boundary of cave roof is simply supposed to be supported according to the integrity of the rock mass in the boundary of cave roof. Thirdly, based on the theory of plates and shells, the simplified model is calculated and the theoretical calculation formula to determine the safe thickness of cave roof under pile tip can be obtained when the edges of the cave roof are simply supported. In the end, the analysis of the practical engineering project proves the feasibility and the rationality of the method which can be a new method to calculate the safe thickness of cave roof under pile tip. 展开更多
关键词 KARST cave roof theory of plates and shells PILE safe thickness
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Electron bandstructure of kaolinite and its mechanism of flotation using dodecylamine as collector 被引量:1
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作者 夏柳荫 钟宏 +1 位作者 刘广义 李鑫钢 《Journal of Central South University》 SCIE EI CAS 2009年第1期73-79,共7页
The bulk electronic structure of kaolinite (001) plane was studied with quantum mechanical calculations. The CASTEP parameterization of ultrasoft pseudopotentials without core corrections was used to optimize the stru... The bulk electronic structure of kaolinite (001) plane was studied with quantum mechanical calculations. The CASTEP parameterization of ultrasoft pseudopotentials without core corrections was used to optimize the structure of kaolinite bulk and slab models. The results show that Fermi energy of kaolinite (001) plane is 3.05 eV, and the band gap is 4.52 eV. The partial density of states (PDOS) of kaolinite (001) plane indicates that Al-O and Si-O bonds on the mineral surface are highly polar. The oxygen atoms of hydroxyl groups in surface layer are capable of forming hydrogen bond with the head group of cationic collectors. The properties of dodecylamine (DDA) cation were also calculated by density function theory (DFT) method at B3LYP/6-31G (d) level for illuminating the flotation processes of kaolinite. Besides the electrostatic attraction, the mechanism between kaolinite and DDA is found to be hydrogen bonds under acidic condition. 展开更多
关键词 KAOLINITE electron bandstructure FLOTATION quantum mechanical calculations
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Multi-physics analysis of permanent magnet tubular linear motors under severe volumetric and thermal constraints 被引量:2
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作者 李方 叶佩青 张辉 《Journal of Central South University》 SCIE EI CAS CSCD 2016年第7期1690-1699,共10页
Permanent magnet tubular linear motors(TLMs) arranged in multiple rows and multiple columns used for a radiotherapy machine were studied. Due to severe volumetric and thermal constraints, the TLMs were at high risk of... Permanent magnet tubular linear motors(TLMs) arranged in multiple rows and multiple columns used for a radiotherapy machine were studied. Due to severe volumetric and thermal constraints, the TLMs were at high risk of overheating. To predict the performance of the TLMs accurately, a multi-physics analysis approach was proposed. Specifically, it considered the coupling effects amongst the electromagnetic and the thermal models of the TLMs, as well as the fluid model of the surrounding air. To reduce computation cost, both the electromagnetic and the thermal models were based on lumped-parameter methods. Only a minimum set of numerical computation(computational fluid dynamics, CFD) was performed to model the complex fluid behavior. With the proposed approach, both steady state and transient state temperature distributions, thermal rating and permissible load can be predicted. The validity of this approach is verified through the experiment. 展开更多
关键词 tubular linear motor MULTI-PHYSICS COUPLING lumped-parameter temperature prediction
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Mechanical analysis of fixed geosynthetic technique of GRPS embankment
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作者 张军 郑俊杰 马强 《Journal of Central South University》 SCIE EI CAS 2013年第5期1368-1375,共8页
To overcome the deficiencies of conventional geosynthetic-reinforced and pile-supported (GRPS) embankment, a new improvement technique, fixed geosynthetic technique of GRPS embankment (FGT embankment), was developed a... To overcome the deficiencies of conventional geosynthetic-reinforced and pile-supported (GRPS) embankment, a new improvement technique, fixed geosynthetic technique of GRPS embankment (FGT embankment), was developed and introduced. Based on the discussion about the load transfer mechanism of FGT embankment, a simplified check method of the requirement of geosynthetic tensile strength and a mechanical model of the FGT embankment were proposed. Two conditions, the pile cap and pile beam conditions are considered in the mechanical model. The finite difference method is used to solve the mechanical model owing to the complexity of the differential equations and the soil strata. Then, the numerical procedure is programmed. Finally, a field test is conducted to verify the mechanical model and the calculated results are in good agreement with field measured data. 展开更多
关键词 mechanical model load transfer mechanism fixed geosynthetic technique GRPS embankment field test
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