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辽宁省2004-2008年布鲁氏菌病流行病学现状及分型分析 被引量:16
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作者 毛玲玲 姚文清 +7 位作者 耿英芝 秦彩明 李鑫 孙广玖 刘权恕 张旭 李松岩 陈静乙 《中国人兽共患病学报》 CAS CSCD 北大核心 2010年第12期1170-1172,共3页
关键词 布鲁氏菌病 分型分析 流行病学 人兽共患传染病 辽宁省 细菌侵入 变态反应性 布鲁氏菌属
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内蒙古炭疽芽胞杆菌多位点串联重复序列分型分析 被引量:4
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作者 海岩 王文瑞 +5 位作者 郭卫东 李昕 跃华 张慧娟 魏建春 武利涛 《中国人兽共患病学报》 CAS CSCD 北大核心 2014年第9期975-977,共3页
炭疽芽胞杆菌是引起人和动物炭疽的病原菌,牛、羊等食草动物为主要传染源,人类主要通过接触炭疽病畜毛皮和食肉而感染,也可以通过吸入含有炭疽芽孢的粉尘或气溶胶而感染.我国炭疽自然疫源地分布广泛,炭疽病例时有发生.内蒙古地区是主要... 炭疽芽胞杆菌是引起人和动物炭疽的病原菌,牛、羊等食草动物为主要传染源,人类主要通过接触炭疽病畜毛皮和食肉而感染,也可以通过吸入含有炭疽芽孢的粉尘或气溶胶而感染.我国炭疽自然疫源地分布广泛,炭疽病例时有发生.内蒙古地区是主要的炭疽自然疫源地,每年都有病例发生.炭疽杆菌是一种相对保守的细菌,很多基因分型方法都不能对其分型,Keim等[1]首次将MLVA8方法用于炭疽芽抱杆菌基因分子分型中,其选择6个染色体上VNTR多态性位点和2个质粒上VNTR多态性位点联合建立了MLVA8分析方法,是比较有效的区别不同炭疽芽胞杆菌的方法. 展开更多
关键词 炭疽芽胞杆菌 内蒙古地区 分型分析 串联重复序列 基因分型方法 自然疫源地 多态性位点 VNTR
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婴幼儿配方羊乳粉生产环节蜡样芽孢杆菌分离株多位点序列分型分析 被引量:2
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作者 刘阳 葛武鹏 +5 位作者 张静 郭春锋 梁秀珍 王智 张兴吉 王瑞 《食品科学》 EI CAS CSCD 北大核心 2018年第14期166-171,共6页
为探讨我国婴幼儿配方羊乳粉生产环节阳性分离株的遗传多样性,并将其基因序列与已报道的乳源分离株的基因序列进行比对,确立其特征序列,为婴幼儿配方食品生产体系溯源提供依据,为探究其致病机理和有效防控提供参考依据。实验选取glpF、... 为探讨我国婴幼儿配方羊乳粉生产环节阳性分离株的遗传多样性,并将其基因序列与已报道的乳源分离株的基因序列进行比对,确立其特征序列,为婴幼儿配方食品生产体系溯源提供依据,为探究其致病机理和有效防控提供参考依据。实验选取glpF、gmk、ilvD、pta、pur、pycA、tpi 7个管家基因构建多位点序列分析分型方案,鉴定经随机扩增多态性DNA分型得到的42株Bacillus cereus的序列类型。结果表明:42株B.cereus分为7个序列类型(sequence type,ST),分别为ST-770(4.8%,2/42)、ST-1000(71.4%,30/42)、ST-1084(9.5%,4/42)、ST-1348(2.4%,1/42)、ST-1349(2.4%,1/42)、ST-1350(2.4%,1/42)和ST-1351(7.1%,3/42);所有分离株被识别为205、142、23三个不同克隆谱系,2个单态群(ST-770和ST-1351)和2个独株(ST-1348和ST-1349);发现4个新的ST型和4个新的等位基因,已上传至国际数据库得到新的序列号和等位基因号,分别为ST-1348、ST-1349、ST-1350、ST-1351和glp-253、glp-254、ilv-277、pyc-199。 展开更多
关键词 蜡样芽孢杆菌(Bacillus cereus) 婴幼儿配方羊乳粉 多位点序列分析分型(MLST) 基因序列(ST)
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一株致病性血清2型猪链球菌的多位点序列分型及其灭活后对小鼠的免疫保护效果评价
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作者 高明明 王淑杰 +9 位作者 金家民 刘永刚 陶冶 李爱东 张璐 李莉 姜成刚 王刚 申红 蔡雪辉 《中国预防兽医学报》 CAS CSCD 北大核心 2015年第1期57-61,共5页
为鉴定一株2012年分离自广东省的致病菌并评价其免疫原性,本研究对该分离株进行分型鉴定和MSLT分型分析。鉴定结果显示,该分离株为血清2型猪链球菌(S.suis);其MSLT分型为ST1型,等位基因图谱是1、1、1、1、1、1、1,与高致病性的世界流行... 为鉴定一株2012年分离自广东省的致病菌并评价其免疫原性,本研究对该分离株进行分型鉴定和MSLT分型分析。鉴定结果显示,该分离株为血清2型猪链球菌(S.suis);其MSLT分型为ST1型,等位基因图谱是1、1、1、1、1、1、1,与高致病性的世界流行株P1/7同型;毒力基因型为Mrp+/Ef+/Sly+,将其命名为GD。进一步使用GD灭活菌以3×108cfu剂量3次免疫小鼠,免疫后分别采用GD,7型、9型S.suis WH和ZD进行攻毒试验。动物试验结果显示,免疫后小鼠抗体水平显著升高;对GD、WH和ZD的保护率分别为87.5%、87.5%和75%;免疫组小鼠血液和脏器内的细菌载量均显著低于对照组,而且无明显病变。本研究结果表明,GD灭活疫苗可以对不同血清型S.suis攻毒提供有效的免疫保护,可以作为预防S.suis病的有效灭活疫苗候选株。 展开更多
关键词 猪链球菌 MLST分型分析 灭活疫苗 免疫保护
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Numerical Analysis of Steady Smoldering of Biomass Rods
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作者 Zhao Wentao Yu Guangxin +3 位作者 Zhang Yi Wang Youtang Zhou Dan He Fang 《燃烧科学与技术》 CAS CSCD 北大核心 2024年第5期507-519,共13页
Understanding the steady mechanism of biomass smoldering plays a great role in the utilization of smoldering technology.In this study numerical analysis of steady smoldering of biomass rods was performed.A two-dimensi... Understanding the steady mechanism of biomass smoldering plays a great role in the utilization of smoldering technology.In this study numerical analysis of steady smoldering of biomass rods was performed.A two-dimensional(2D)steady model taking into account both char oxidation and pyrolysis was developed on the basis of a calculated propagation velocity according to empirical correlation.The model was validated against the smoldering experiment of biomass rods under natural conditions,and the maximum error was smaller than 31%.Parameter sensitivity analysis found that propagation velocity decreases significantly while oxidation area and pyrolysis zone increase significantly with the increasing diameter of rod fuel. 展开更多
关键词 steady smoldering biomass rod numerical analysis 2D steady model sensitivity analysis
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Delamination analysis of woven fabrication laminates using cohesive zone model 被引量:2
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作者 Mohsen Moslemi Mohammadreza Khoshravan azar 《Journal of Central South University》 SCIE EI CAS CSCD 2016年第1期27-38,共12页
A new test method was proposed to evaluate the cohesive strength of composite laminates. Cohesive strength and the critical strain energy for Mode-II interlamiar fracture of E-glass/epoxy woven fabrication were determ... A new test method was proposed to evaluate the cohesive strength of composite laminates. Cohesive strength and the critical strain energy for Mode-II interlamiar fracture of E-glass/epoxy woven fabrication were determined from the single lap joint(SLJ) and end notch flexure(ENF) test, respectively. In order to verify their adequacy, a cohesive zone model simulation based on interface finite elements was performed. A closed form solution for determination of the penalty stiffness parameter was proposed. Modified form of Park-Paulino-Roesler traction-separation law was provided and conducted altogether with trapezoidal and bilinear mixed-mode damage models to simulate damage using Abaqus cohesive elements. It was observed that accurate damage prediction and numerical convergence were obtained using the proposed penalty stiffness. Comparison between three damage models reveals that good simulation of fracture process zone and delamination prediction were obtained using the modified PPR model as damage model. Cohesive zone length as a material property was determined. To ensure the sufficient dissipation of energy, it was recommended that at least 4 elements should span cohesive zone length. 展开更多
关键词 cohesive zone model DELAMINATION cohesive strength finite element prediction
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Finite difference method for dynamic response analysis of anchorage system 被引量:6
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作者 言志信 段建 +3 位作者 江平 刘子振 赵红亮 黄文贵 《Journal of Central South University》 SCIE EI CAS 2014年第3期1098-1106,共9页
Based on some assumptions, the dynamic analysis model of anchorage system is established. The dynamic governing equation is expressed as finite difference format and programmed by using MATLAB language. Compared with ... Based on some assumptions, the dynamic analysis model of anchorage system is established. The dynamic governing equation is expressed as finite difference format and programmed by using MATLAB language. Compared with theoretical method, the finite difference method has been verified to be feasible by a case study. It is found that under seismic loading, the dynamic response of anchorage system is synchronously fluctuated with the seismic vibration. The change of displacement amplitude of material points is slight, and comparatively speaking, the displacement amplitude of the outside point is a little larger than that of the inside point, which shows amplification effect of surface. While the axial force amplitude transforms considerably from the inside to the outside. It increases first and reaches the peak value in the intersection between the anchoring section and free section, then decreases slowly in the free section. When considering damping effect of anchorage system, the finite difference method can reflect the time attenuation characteristic better, and the calculating result would be safer and more reasonable than the dynamic steady-state theoretical method. What is more, the finite difference method can be applied to the dynamic response analysis of harmonic and seismic random vibration for all kinds of anchor, and hence has a broad application prospect. 展开更多
关键词 anchorage system dynamic response finite difference method attenuation characteristic
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FEM modeling for 3D dynamic analysis of deep-ocean mining pipeline and its experimental verification 被引量:6
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作者 王刚 刘少军 李力 《Journal of Central South University of Technology》 EI 2007年第6期808-813,共6页
3D dynamic analysis models of 1000 m deep-ocean mining pipeline, including steel lift pipe, pump, buffer and flexible hose, were established by finite element method (FEM). The coupling effect of steel lift pipe and f... 3D dynamic analysis models of 1000 m deep-ocean mining pipeline, including steel lift pipe, pump, buffer and flexible hose, were established by finite element method (FEM). The coupling effect of steel lift pipe and flexible hose, and main external loads of pipeline were considered in the models, such as gravity, buoyancy, hydrodynamic forces, internal and external fluid pressures, concentrated suspension buoyancy on the flexible hose, torsional moment and axial force induced by pump working. Some relevant FEM models and solution techniques were developed, according to various 3D transient behaviors of integrated deep-ocean mining pipeline, including towing motions of track-keeping operation and launch process of pipeline. Meanwhile, an experimental verification system in towing water tank that had similar characteristics of designed mining pipeline was developed to verify the accuracy of the FEM models and dynamic simulation. The experiment results show that the experimental records and simulation results of stress of pipe are coincided. Based on the further simulations of 1 000 m deep-ocean mining pipeline, the simulation results show that, to form configuration of a saddle shape, the total concentrated suspension buoyancy of flexible hose should be 95%?105% of the gravity of flexible hose in water, the first suspension point occupies 1/3 of the total buoyancy, and the second suspension point occupies 2/3 of the total buoyancy. When towing velocity of mining system is less than 0.5 m/s, the towing track of buffer is coincided with the setting route of ship on the whole and the configuration of flexible hose is also kept well. 展开更多
关键词 deep-ocean mining pipeline modeling dynamic analysis finite element method
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Development of analysis model for geometric error in turning processes 被引量:1
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作者 LEE Mun-jae LEE Choon-man 《Journal of Central South University》 SCIE EI CAS 2011年第3期711-717,共7页
A finite element model was established for analyzing the geometric errors in turning operations and a two-step analyzing process was proposed. In the first analyzing step, the cutting force and the cutting heat for th... A finite element model was established for analyzing the geometric errors in turning operations and a two-step analyzing process was proposed. In the first analyzing step, the cutting force and the cutting heat for the cutting conditions were obtained using the AdvantEdge. Also, the deformation of a workpiece was estimated in the second step using the ANSYS. The deformation was analyzed for a 150 mm-long workpiece at three different measuring points, such as 10, 70 and 130 mm from a reference point, and the amounts of the deformation were compared through experiments. /n the results of the comparison and analysis, the values obtained from these comparison and analysis represent similar tendencies. Also, it is verified that their geometric errors increase with the increase in temperature. In addition, regarding the factors that affect the deformation of a workpiecc, it can be seen that the geometric error in the lathe is about 15%, the error caused by the cutting force is about 10%, and the deformation caused by the heat is about 75%. 展开更多
关键词 geometric error machining accuracy finite element analysis TURNING cutting force cutting temperature
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Effects of h-BN content on properties of Ni-Cr/h-BN composite 被引量:1
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作者 韦小凤 王日初 +2 位作者 冯艳 彭超群 朱学卫 《Journal of Central South University》 SCIE EI CAS 2011年第5期1334-1339,共6页
Ni-Cr/h-BN self-lubricating composities were prepared by powder metallurgy (P/M) method.The effects of hexagonal boron nitride (h-BN) content on the mechanical and tribological properties of the Ni-Cr/h-BN composites ... Ni-Cr/h-BN self-lubricating composities were prepared by powder metallurgy (P/M) method.The effects of hexagonal boron nitride (h-BN) content on the mechanical and tribological properties of the Ni-Cr/h-BN composites were investigated.The corresponding frictional models were established to analyze the formation of the lubricant h-BN films on the surfaces of the Ni-Cr/h-BN composites.The results show that,when the content of h-BN increases from 5% to 15% (mass fraction),the bending strength of the Ni-Cr/h-BN composite decreases from 96.670 MPa to 17.319 MPa,and the hardness (HB) decreases from 33 to 14.The friction coefficient of the Ni-Cr/h-BN composite decreases firstly from 0.385 to 0.216,and then increases to 0.284,while the wear rate decreases firstly from 4.14×10-9 kg/(N·m) to 1.35×10-9 kg/(N·m),then increases to 2.36×10-9 kg/(N·m).The best comprehensive mechanical and tribological properties can be obtained between 10% and 12% h-BN addition. 展开更多
关键词 Ni-Cr/h-BN composite hexagonal boron nitride solid lubricating film friction coefficient wear rate
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Real-time lane departure warning system based on principal component analysis of grayscale distribution and risk evaluation model 被引量:4
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作者 张伟伟 宋晓琳 张桂香 《Journal of Central South University》 SCIE EI CAS 2014年第4期1633-1642,共10页
A technology for unintended lane departure warning was proposed. As crucial information, lane boundaries were detected based on principal component analysis of grayscale distribution in search bars of given number and... A technology for unintended lane departure warning was proposed. As crucial information, lane boundaries were detected based on principal component analysis of grayscale distribution in search bars of given number and then each search bar was tracked using Kalman filter between frames. The lane detection performance was evaluated and demonstrated in ways of receiver operating characteristic, dice similarity coefficient and real-time performance. For lane departure detection, a lane departure risk evaluation model based on lasting time and frequency was effectively executed on the ARM-based platform. Experimental results indicate that the algorithm generates satisfactory lane detection results under different traffic and lighting conditions, and the proposed warning mechanism sends effective warning signals, avoiding most false warning. 展开更多
关键词 lane departure warning system lane detection lane tracking principal component analysis risk evaluation model ARM-based real-time system
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Temperature gradients in concrete box girder bridge under effect of cold wave 被引量:3
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作者 顾斌 陈志坚 陈欣迪 《Journal of Central South University》 SCIE EI CAS 2014年第3期1227-1241,共15页
The temperature distributions of a prestressed concrete box girder bridge under the effect of cold wave processes were analyzed. The distributions were found different from those under the effect of solar radiation or... The temperature distributions of a prestressed concrete box girder bridge under the effect of cold wave processes were analyzed. The distributions were found different from those under the effect of solar radiation or nighttime radiation cooling and should not be simplified as one dimensional. A temperature predicting model that can accurately predict temperatures over the cross section of the concrete box girder was developed. On the basis of the analytical model, a two-dimensional temperature gradient model was proposed and a parametric study that considered meteorological factors was performed. The results of sensitivity analysis show that the cold wave with shorter duration and more severe temperature drop may cause more unfavorable influences on the concrete box girder bridge. Finally, the unrestrained linear curvatures, self-equilibrating stresses and bending stresses when considering the frame action of the cross section, were derived from the proposed temperature gradient model and current code provisions, respectively. Then, a comparison was made between the value calculated against proposed model and several current specifications. The results show that the cold wave may cause more unfavorable effect on the concrete box girder bridge, especially on the large concrete box girder bridge. Therefore, it is necessary to consider the thermal effect caused by cold wave during the design stage. 展开更多
关键词 concrete box girder temperature field temperature gradient cold wave
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Short-term forecasting optimization algorithms for wind speed along Qinghai-Tibet railway based on different intelligent modeling theories 被引量:8
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作者 刘辉 田红旗 李燕飞 《Journal of Central South University》 SCIE EI CAS 2009年第4期690-696,共7页
To protect trains against strong cross-wind along Qinghai-Tibet railway, a strong wind speed monitoring and warning system was developed. And to obtain high-precision wind speed short-term forecasting values for the s... To protect trains against strong cross-wind along Qinghai-Tibet railway, a strong wind speed monitoring and warning system was developed. And to obtain high-precision wind speed short-term forecasting values for the system to make more accurate scheduling decision, two optimization algorithms were proposed. Using them to make calculative examples for actual wind speed time series from the 18th meteorological station, the results show that: the optimization algorithm based on wavelet analysis method and improved time series analysis method can attain high-precision multi-step forecasting values, the mean relative errors of one-step, three-step, five-step and ten-step forecasting are only 0.30%, 0.75%, 1.15% and 1.65%, respectively. The optimization algorithm based on wavelet analysis method and Kalman time series analysis method can obtain high-precision one-step forecasting values, the mean relative error of one-step forecasting is reduced by 61.67% to 0.115%. The two optimization algorithms both maintain the modeling simple character, and can attain prediction explicit equations after modeling calculation. 展开更多
关键词 train safety wind speed forecasting wavelet analysis time series analysis Kalman filter optimization algorithm
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Reliability analysis of laminated composite under compression and shear loads 被引量:1
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作者 王富生 张钧然 +2 位作者 王佩艳 霍世慧 岳珠峰 《Journal of Central South University》 SCIE EI CAS 2012年第10期2712-2717,共6页
Carrying on a series of compression and shear tests by a large number of specimens, reliabilities of T300/QY8911 laminated composite were studied when dispersibility models were described. The results show that the st... Carrying on a series of compression and shear tests by a large number of specimens, reliabilities of T300/QY8911 laminated composite were studied when dispersibility models were described. The results show that the stress is linearly dependent on the strain and the damage modes of specimens are brittle fracture for both kinds of tests. Dispersibility models of compression and shear strength are expressed as Re-N(415.39, 6 586.36) and Rs-ln(5.071 8, 0.155 3), respectively. When normal and lognormal distributions were used to describe the dispersibility models of compression and shear strength, and the compression or shear load follows the normal distribution, the almost same failure probability can be obtained from different reliability analysis methods. 展开更多
关键词 laminated composite dispersibility model reliability analysis compression SHEAR brittle fracture failure probability
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Thermo-mechanical analysis of an SI engine piston using different boundary condition treatments
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作者 Amir-Hasan Kakaee Javad Gharloghi +1 位作者 Aliasghar Foroughifar Abdoreza Khanlari 《Journal of Central South University》 SCIE EI CAS CSCD 2015年第10期3817-3829,共13页
Heat transfer of an SI engine's piston is calculated by employing three different methods based on resistor-capacitor model with the help of MATLAB code, and then the piston is thermo-mechanically analyzed using c... Heat transfer of an SI engine's piston is calculated by employing three different methods based on resistor-capacitor model with the help of MATLAB code, and then the piston is thermo-mechanically analyzed using commercial ANSYS code. The results of three methods are compared to study their effects on the piston thermal behavior. It is shown that resistor-capacitor model with less number of equations and consequently less solution time, is an appropriate method for solving problems of engine piston heat transfer. In the second part, the thermal stresses due to non-uniform temperature distribution, and mechanical stresses due to mechanical loads are calculated. Finally, the temperature distributions as a thermal load along with mechanical loads are applied to the piston to determine the total stress distribution and critical fracture zones. It is found that the amount of thermal stresses is considerable. 展开更多
关键词 PISTON TEMPERATURE boundary condition STRESS thermo-mechanical analysis
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Mechanical analysis of fixed geosynthetic technique of GRPS embankment
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作者 张军 郑俊杰 马强 《Journal of Central South University》 SCIE EI CAS 2013年第5期1368-1375,共8页
To overcome the deficiencies of conventional geosynthetic-reinforced and pile-supported (GRPS) embankment, a new improvement technique, fixed geosynthetic technique of GRPS embankment (FGT embankment), was developed a... To overcome the deficiencies of conventional geosynthetic-reinforced and pile-supported (GRPS) embankment, a new improvement technique, fixed geosynthetic technique of GRPS embankment (FGT embankment), was developed and introduced. Based on the discussion about the load transfer mechanism of FGT embankment, a simplified check method of the requirement of geosynthetic tensile strength and a mechanical model of the FGT embankment were proposed. Two conditions, the pile cap and pile beam conditions are considered in the mechanical model. The finite difference method is used to solve the mechanical model owing to the complexity of the differential equations and the soil strata. Then, the numerical procedure is programmed. Finally, a field test is conducted to verify the mechanical model and the calculated results are in good agreement with field measured data. 展开更多
关键词 mechanical model load transfer mechanism fixed geosynthetic technique GRPS embankment field test
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