目的探讨脉络宁口服液联合丁苯酞对血管性痴呆患者认知功能及内质网应激反应相关指标的影响。方法选取2018年5月—2020年4月医院血管性痴呆患者129例,简单随机化分组,各43例。对照A组予以丁苯酞,对照B组予以脉络宁口服液,观察组予以脉...目的探讨脉络宁口服液联合丁苯酞对血管性痴呆患者认知功能及内质网应激反应相关指标的影响。方法选取2018年5月—2020年4月医院血管性痴呆患者129例,简单随机化分组,各43例。对照A组予以丁苯酞,对照B组予以脉络宁口服液,观察组予以脉络宁口服液联合丁苯酞。观察统计3组临床疗效、不良反应、外周血氧化应激指标[超氧化物歧化酶(Superoxide dismutase,SOD)、丙二醛(Malondialdehyde,MDA)]、内质网应激反应相关指标[葡萄糖调节蛋白78(Glucose regulated protein 78ku,CRP78)、天冬半胱氨酸特异性蛋白酶-12(Cysteine-containing aspartate-specific proteases-12,Caspase-12)、CCAAT/增强子结合蛋白(Ccaat/enhancer binding protein,CHOP)]、蒙特利尔认知功能评估量表(Montreal Cognitive Assessment Scale,MoCA)、简易精神状态检查表(Mini-Mental State Examination,MMSE)、日常生活能力量表(Activity of Daily Living Scale,ADL)、中风专用生活质量量表(stroke specific quality of life scale,SS-QOL)评分。结果观察组治疗总有效率较对照A、B组高(P<0.05);观察组疗程结束后MoCA、MMSE、ADL、SS-QOL评分高于对照A、B组,差异有统计学意义(P<0.05);观察组疗程结束后血清SOD水平高于对照A、B组,MDA、GRP78、CHOP、Caspase-12水平低于对照A、B组,差异有统计学意义(P<0.05);3组不良反应发生率相比,差异无统计学意义(P>0.05)。结论血管性痴呆患者采用脉络宁口服液、丁苯酞联合治疗,有助于改善认知功能、日常生活能力,还可有效抑制内质网应激,抵抗氧化应激,效果较为显著,且安全性高。展开更多
肿瘤微环境不同于正常组织的生理环境,其表现为低氧、低糖及炎性细胞浸润等,而肿瘤细胞如何在这种不利细胞生长的条件下生存呢?最近康奈尔大学的Glimcher教授发表在Nature上的论文表明,在低氧条件下,三阴性乳腺癌(triple negative bre...肿瘤微环境不同于正常组织的生理环境,其表现为低氧、低糖及炎性细胞浸润等,而肿瘤细胞如何在这种不利细胞生长的条件下生存呢?最近康奈尔大学的Glimcher教授发表在Nature上的论文表明,在低氧条件下,三阴性乳腺癌(triple negative breast cancer,TNBC)细胞内质网应激反应信号通路中的内质网跨膜蛋白肌醇酶1α(inositol-requiring enzyme 1α,IRE1α)、内质网类似激酶(PKR like ER kinase,PERK)被活化,活化的IREa1、PERK通过剪切X-盒结合蛋白1(X-box binding protein 1,XBP1)mRNA产生剪切体XBP1s,XBP1s和另外一个低氧诱导的转录因子缺氧诱导因子1a(hypoxia-inducible factor-1a,HIF1a)在细胞核内结合,协同HIF1a的目的基因转录,促进三阴性肿瘤细胞的生长和复发。展开更多
Fatty liver fibrosis, a severe type of Nonalcoholic fatty liver disease (NAFLD), can be reversed by effec- tive medical intervention. Endoplasmic reticulum stress promotes liver fat accumulation and induction of in...Fatty liver fibrosis, a severe type of Nonalcoholic fatty liver disease (NAFLD), can be reversed by effec- tive medical intervention. Endoplasmic reticulum stress promotes liver fat accumulation and induction of inflammatory cytokines, which plays an important role in the process of liver fibrosis. Our previous study have shown that, SPJ (saponins of Panax Japonicus) has significant antioxidant and anti-inflammatory activity. Therefore, the protective effects and mechanisms of SPJ against fatty liver fibrosis in mice were investigated. In this study, fatty liver fibrosis was induced by high-fat diet combined with intraperitoneal injection of heterologous protein. The results showed that SPJ significantly improved liver function and decreased the serum lipid level. SPJ remarkly decreased the liver steato- sis, collagen fibers and inflammatory cell infiltration. Furthermore, treatment with SPJ significantly downregulated the mRNA level of Collagen Type 1α (COLL 1α), α-SMA, TIMP, CHOP and GRP78. In addition, SPJ significantly de- creased the protein level of p-JNK, COLL 1α and GRP78. Taken together, SPJ protect against fatty liver fibrosis through inhibition of endoplasmic reticulum stress response and CHOP and JNK-mediated apoptosis and inflammation pathway. These results indicate that SPJ might be a potential drug for prevention of liver fibrosis.展开更多
文摘目的探讨脉络宁口服液联合丁苯酞对血管性痴呆患者认知功能及内质网应激反应相关指标的影响。方法选取2018年5月—2020年4月医院血管性痴呆患者129例,简单随机化分组,各43例。对照A组予以丁苯酞,对照B组予以脉络宁口服液,观察组予以脉络宁口服液联合丁苯酞。观察统计3组临床疗效、不良反应、外周血氧化应激指标[超氧化物歧化酶(Superoxide dismutase,SOD)、丙二醛(Malondialdehyde,MDA)]、内质网应激反应相关指标[葡萄糖调节蛋白78(Glucose regulated protein 78ku,CRP78)、天冬半胱氨酸特异性蛋白酶-12(Cysteine-containing aspartate-specific proteases-12,Caspase-12)、CCAAT/增强子结合蛋白(Ccaat/enhancer binding protein,CHOP)]、蒙特利尔认知功能评估量表(Montreal Cognitive Assessment Scale,MoCA)、简易精神状态检查表(Mini-Mental State Examination,MMSE)、日常生活能力量表(Activity of Daily Living Scale,ADL)、中风专用生活质量量表(stroke specific quality of life scale,SS-QOL)评分。结果观察组治疗总有效率较对照A、B组高(P<0.05);观察组疗程结束后MoCA、MMSE、ADL、SS-QOL评分高于对照A、B组,差异有统计学意义(P<0.05);观察组疗程结束后血清SOD水平高于对照A、B组,MDA、GRP78、CHOP、Caspase-12水平低于对照A、B组,差异有统计学意义(P<0.05);3组不良反应发生率相比,差异无统计学意义(P>0.05)。结论血管性痴呆患者采用脉络宁口服液、丁苯酞联合治疗,有助于改善认知功能、日常生活能力,还可有效抑制内质网应激,抵抗氧化应激,效果较为显著,且安全性高。
文摘肿瘤微环境不同于正常组织的生理环境,其表现为低氧、低糖及炎性细胞浸润等,而肿瘤细胞如何在这种不利细胞生长的条件下生存呢?最近康奈尔大学的Glimcher教授发表在Nature上的论文表明,在低氧条件下,三阴性乳腺癌(triple negative breast cancer,TNBC)细胞内质网应激反应信号通路中的内质网跨膜蛋白肌醇酶1α(inositol-requiring enzyme 1α,IRE1α)、内质网类似激酶(PKR like ER kinase,PERK)被活化,活化的IREa1、PERK通过剪切X-盒结合蛋白1(X-box binding protein 1,XBP1)mRNA产生剪切体XBP1s,XBP1s和另外一个低氧诱导的转录因子缺氧诱导因子1a(hypoxia-inducible factor-1a,HIF1a)在细胞核内结合,协同HIF1a的目的基因转录,促进三阴性肿瘤细胞的生长和复发。
文摘Fatty liver fibrosis, a severe type of Nonalcoholic fatty liver disease (NAFLD), can be reversed by effec- tive medical intervention. Endoplasmic reticulum stress promotes liver fat accumulation and induction of inflammatory cytokines, which plays an important role in the process of liver fibrosis. Our previous study have shown that, SPJ (saponins of Panax Japonicus) has significant antioxidant and anti-inflammatory activity. Therefore, the protective effects and mechanisms of SPJ against fatty liver fibrosis in mice were investigated. In this study, fatty liver fibrosis was induced by high-fat diet combined with intraperitoneal injection of heterologous protein. The results showed that SPJ significantly improved liver function and decreased the serum lipid level. SPJ remarkly decreased the liver steato- sis, collagen fibers and inflammatory cell infiltration. Furthermore, treatment with SPJ significantly downregulated the mRNA level of Collagen Type 1α (COLL 1α), α-SMA, TIMP, CHOP and GRP78. In addition, SPJ significantly de- creased the protein level of p-JNK, COLL 1α and GRP78. Taken together, SPJ protect against fatty liver fibrosis through inhibition of endoplasmic reticulum stress response and CHOP and JNK-mediated apoptosis and inflammation pathway. These results indicate that SPJ might be a potential drug for prevention of liver fibrosis.