Five major disaccharide peaks were obtained from the digests of heparin by a novel heparinase from the species of Sphingobacterium on the high performance liquid chromatography (HPLC). The electrospray ionization-mass...Five major disaccharide peaks were obtained from the digests of heparin by a novel heparinase from the species of Sphingobacterium on the high performance liquid chromatography (HPLC). The electrospray ionization-mass spectrometry of peak I to V sequentially represented one nonsulfated disaccharide, two monosulfated disaccharides,two disulfated disaccharides. No trisulfated disaccharide was detected. The findings revealed that the heparinase specifically eliminated undersulfated domain in heparin.展开更多
文摘Five major disaccharide peaks were obtained from the digests of heparin by a novel heparinase from the species of Sphingobacterium on the high performance liquid chromatography (HPLC). The electrospray ionization-mass spectrometry of peak I to V sequentially represented one nonsulfated disaccharide, two monosulfated disaccharides,two disulfated disaccharides. No trisulfated disaccharide was detected. The findings revealed that the heparinase specifically eliminated undersulfated domain in heparin.
