Oligodeoxynucleotide from SARS virus was selected and synthesized. Its complexes with alkaloid compounds were investigated by electrospray mass spectrometry. We found that three alkaloid compounds could interact with ...Oligodeoxynucleotide from SARS virus was selected and synthesized. Its complexes with alkaloid compounds were investigated by electrospray mass spectrometry. We found that three alkaloid compounds could interact with the DNA target molecule in five alkaloids analyzed. With increasing molar ratio of the three alkaloid compounds, the complexes between alkaloids and DNA with different stoichiometric ratios were found in their MS spectra. The order of the alkalinity of their gas phase corresponds to that of liquid phase, that is: Palmatine>Jatrorrhizine>Berberin. The relative abundance of these complexes in mass spectra can assess the relative affinities of above three alkaloid compounds with the DNA target molecule. In this paper, the binding sites between jatrorrhizine and DNA was deduced from the interaction experiments of jatrorrhizine with three nucleosides.展开更多
文摘Oligodeoxynucleotide from SARS virus was selected and synthesized. Its complexes with alkaloid compounds were investigated by electrospray mass spectrometry. We found that three alkaloid compounds could interact with the DNA target molecule in five alkaloids analyzed. With increasing molar ratio of the three alkaloid compounds, the complexes between alkaloids and DNA with different stoichiometric ratios were found in their MS spectra. The order of the alkalinity of their gas phase corresponds to that of liquid phase, that is: Palmatine>Jatrorrhizine>Berberin. The relative abundance of these complexes in mass spectra can assess the relative affinities of above three alkaloid compounds with the DNA target molecule. In this paper, the binding sites between jatrorrhizine and DNA was deduced from the interaction experiments of jatrorrhizine with three nucleosides.
