A novel 1.3 kb cDNA was amplified from cDNA library of Lycium barbarum by PCR.This cDNA clone was further inserted into pGEM-T. The sequence analysis showed that the cDNA was 1 292 bp long with an open reading frame o...A novel 1.3 kb cDNA was amplified from cDNA library of Lycium barbarum by PCR.This cDNA clone was further inserted into pGEM-T. The sequence analysis showed that the cDNA was 1 292 bp long with an open reading frame of 1 275 bp, and encoded a polypeptide of 425 animo acids. Its cDNA sequence showed 75%～80% identity, and the amino acid sequence shared 70%～80% identity with those of PSY from Lycopersicon esculentum, Capsicum annuum, Arabidopsis thaliana and Zea mays respectively, indicating that the full-length PSY cDNA was isolated from L. barbarum.展开更多
文摘A novel 1.3 kb cDNA was amplified from cDNA library of Lycium barbarum by PCR.This cDNA clone was further inserted into pGEM-T. The sequence analysis showed that the cDNA was 1 292 bp long with an open reading frame of 1 275 bp, and encoded a polypeptide of 425 animo acids. Its cDNA sequence showed 75%～80% identity, and the amino acid sequence shared 70%～80% identity with those of PSY from Lycopersicon esculentum, Capsicum annuum, Arabidopsis thaliana and Zea mays respectively, indicating that the full-length PSY cDNA was isolated from L. barbarum.
