目的:探讨阿霉素磁性介孔氧化硅纳米颗粒(Doxorubicin-loading Mesoporous Magnetic Silia Nanoparticles,DOX@M-MSNs)对口腔鳞状细胞癌细胞的体外靶向杀伤作用。方法:采用甲基噻唑基四唑(MTT)法,流式细胞仪及激光共聚焦显微镜检测DOX@M...目的:探讨阿霉素磁性介孔氧化硅纳米颗粒(Doxorubicin-loading Mesoporous Magnetic Silia Nanoparticles,DOX@M-MSNs)对口腔鳞状细胞癌细胞的体外靶向杀伤作用。方法:采用甲基噻唑基四唑(MTT)法,流式细胞仪及激光共聚焦显微镜检测DOX@M-MSNs对CAL-27的体外靶向抗肿瘤效果。结果:单纯磁性介孔氧化硅纳米颗粒表现出非常好的生物稳定性以及生物相容性,尽管浓度为100 mg/L时,细胞存活率仍超过80%。当浓度值有所提升及作用时间延长后,相较于单纯阿霉素原料组,DOX@M-MSNs对于肿瘤细胞的增殖抑制作用明显增强,差异有统计学意义(P<0.05)。流式细胞仪检测结果显示DOX@M-MSNs促进肿瘤细胞的凋亡率高于单纯阿霉素,随着药物作用时间延长,其对肿瘤细胞的抑制效果明显增强,凋亡率增高。激光共聚焦显微镜检测结果显示通过外加磁场,磁性区域的DOX@M-MSN释放的DOX于肿瘤细胞的聚集优于非磁性区域。结论:DOX@M-MSNs纳米颗粒能够选择性进入肿瘤细胞内,从而使得阿霉素对肿瘤细胞的毒性有所增强,进而使得其杀伤肿瘤细胞的效果显著提高,外加磁场可以显著地提升输送DOX@M-MSNs进到肿瘤细胞内的效率,进而提高肿瘤抑制效果。展开更多
A light and temperature dual responsive copolymer,poly(7-(4-vinylbenzy-loxyl)-4-methylcoumarin-co-N vinyl caprolactam-co-tri(ethylene glycol)methyl ether methacrylate)(PVNM),was grafted on the surface of dopamine base...A light and temperature dual responsive copolymer,poly(7-(4-vinylbenzy-loxyl)-4-methylcoumarin-co-N vinyl caprolactam-co-tri(ethylene glycol)methyl ether methacrylate)(PVNM),was grafted on the surface of dopamine based mesoporous silica nanoparticles(MSNs).The resulting polymer brush,MSNs-g-PVNM,was characterized by FT-IR,TEM,TGA and XPS.The dual responsive behaviors of MSNs-g-PVNM were systematically studied.With imidacloprid as the model guest pesticide,the loading percentage and loading efficiency of the polymer brush were determined as 9.2%and 40.6%,respectively.The release efficiency of imidacloprid in MSNs-g-PVNM was the lowest value of 5.4%at 20℃ and 365 nm,and it reached the highest value of 52.4%at 50℃ and 254 nm.The loss percentage of imidacloprid on the leaves contained imidacloprid-loaded MSNs-g-PVNM(8.4%)was much less than that contained only imidacloprid(25.2%)after three rinses.It was confirmed that the release process of imidacloprid was well regulated through changing external conditions such as light and temperature.展开更多
基金Project(21376271)supported by the National Natural Science Foundation of ChinaProject(2016TP1007)supported by the Hunan Provincial Science and Technology Plan Project,ChinaProjects(201810533078,S2020105330395)supported by the Undergraduates Innovative Training Foundation of Central South University,China。
文摘A light and temperature dual responsive copolymer,poly(7-(4-vinylbenzy-loxyl)-4-methylcoumarin-co-N vinyl caprolactam-co-tri(ethylene glycol)methyl ether methacrylate)(PVNM),was grafted on the surface of dopamine based mesoporous silica nanoparticles(MSNs).The resulting polymer brush,MSNs-g-PVNM,was characterized by FT-IR,TEM,TGA and XPS.The dual responsive behaviors of MSNs-g-PVNM were systematically studied.With imidacloprid as the model guest pesticide,the loading percentage and loading efficiency of the polymer brush were determined as 9.2%and 40.6%,respectively.The release efficiency of imidacloprid in MSNs-g-PVNM was the lowest value of 5.4%at 20℃ and 365 nm,and it reached the highest value of 52.4%at 50℃ and 254 nm.The loss percentage of imidacloprid on the leaves contained imidacloprid-loaded MSNs-g-PVNM(8.4%)was much less than that contained only imidacloprid(25.2%)after three rinses.It was confirmed that the release process of imidacloprid was well regulated through changing external conditions such as light and temperature.
