Five guanido packings of affinity chromatography for the separation of urokinase have been synthesized. The separation characteristics and the effect of matrix and ligands on them have been studied. The guanidine deri...Five guanido packings of affinity chromatography for the separation of urokinase have been synthesized. The separation characteristics and the effect of matrix and ligands on them have been studied. The guanidine derivative is a fine ligand, the packings with ligand of arginine are much better than that with ligand of p-ABZ. The polyepoxypropyl methacrylate microsphere is a good matrix. The comprehensive function of packings with ligand of arginine and matrix of polyepoxypropyl methacrylate microsphere is more excellent than Sepharose-p-ABZ.展开更多
A series of affinity media were synthesized by using the oligohistines, His Gly, (His) 3 Gly and (His) 5 Gly as the ligands and the home made monosized, non porous copolymer(CL PGMA) beads containing active epoxide gr...A series of affinity media were synthesized by using the oligohistines, His Gly, (His) 3 Gly and (His) 5 Gly as the ligands and the home made monosized, non porous copolymer(CL PGMA) beads containing active epoxide groups as the support. The affinity chromatographic behavior between IgG and the oligohistines was investigated at pH 3 0, 4 5, 7 0 and 10 0. It showed the specific interaction at pH 4 5. The dissociation constants of IgG and oligohistine media(1H, 3H, 5H) were 3 037×10 -6 , 2 459×10 -6 and 1 905×10 -6 L/mol, respectively. The dissociation constants decreased with increasing the number of histine residue in oligopeptide chains. [WT5HZ]展开更多
以3.0μm无孔单分散亲水性交联聚甲基丙烯酸环氧丙酯树脂为基质,与三嗪染料活性蓝F3GA(Cibacron Blue F3GA)反应,制备出一种固定化染料聚合物高效亲和色谱填料。考察了应用该填料时流动相中的盐离子浓度、有机溶剂及流速等对牛血清白蛋...以3.0μm无孔单分散亲水性交联聚甲基丙烯酸环氧丙酯树脂为基质,与三嗪染料活性蓝F3GA(Cibacron Blue F3GA)反应,制备出一种固定化染料聚合物高效亲和色谱填料。考察了应用该填料时流动相中的盐离子浓度、有机溶剂及流速等对牛血清白蛋白(BSA)和溶菌酶(Lys)保留行为的影响。实验结果表明,该染料亲和填料具有良好的色谱性能。利用前沿色谱法测定了染料与溶菌酶之间的表观解离常数为5.26×10-5mol/L。使用该填料成功地从鸡蛋清和小牛血清中分别分离纯化了Lys和BSA,十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析显示Lys和BSA的纯度分别为95%和92%。展开更多
文摘Five guanido packings of affinity chromatography for the separation of urokinase have been synthesized. The separation characteristics and the effect of matrix and ligands on them have been studied. The guanidine derivative is a fine ligand, the packings with ligand of arginine are much better than that with ligand of p-ABZ. The polyepoxypropyl methacrylate microsphere is a good matrix. The comprehensive function of packings with ligand of arginine and matrix of polyepoxypropyl methacrylate microsphere is more excellent than Sepharose-p-ABZ.
文摘A series of affinity media were synthesized by using the oligohistines, His Gly, (His) 3 Gly and (His) 5 Gly as the ligands and the home made monosized, non porous copolymer(CL PGMA) beads containing active epoxide groups as the support. The affinity chromatographic behavior between IgG and the oligohistines was investigated at pH 3 0, 4 5, 7 0 and 10 0. It showed the specific interaction at pH 4 5. The dissociation constants of IgG and oligohistine media(1H, 3H, 5H) were 3 037×10 -6 , 2 459×10 -6 and 1 905×10 -6 L/mol, respectively. The dissociation constants decreased with increasing the number of histine residue in oligopeptide chains. [WT5HZ]
文摘以3.0μm无孔单分散亲水性交联聚甲基丙烯酸环氧丙酯树脂为基质,与三嗪染料活性蓝F3GA(Cibacron Blue F3GA)反应,制备出一种固定化染料聚合物高效亲和色谱填料。考察了应用该填料时流动相中的盐离子浓度、有机溶剂及流速等对牛血清白蛋白(BSA)和溶菌酶(Lys)保留行为的影响。实验结果表明,该染料亲和填料具有良好的色谱性能。利用前沿色谱法测定了染料与溶菌酶之间的表观解离常数为5.26×10-5mol/L。使用该填料成功地从鸡蛋清和小牛血清中分别分离纯化了Lys和BSA,十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析显示Lys和BSA的纯度分别为95%和92%。
