A normal phase high performance chromatographic method with evaporative light scattering detector(ELSD) and diode array detector(DAD), using n hexane/methanol/ethyl acetate(volume ratio 6∶3∶2 ) as the mobile phase, ...A normal phase high performance chromatographic method with evaporative light scattering detector(ELSD) and diode array detector(DAD), using n hexane/methanol/ethyl acetate(volume ratio 6∶3∶2 ) as the mobile phase, was established for the direct determination of active constituents gastrodin(GA) in the ethanol extracts of Gastrodia elata Blume . An silica gel column Zorbax RX SIL\[4 6 mm (\{i.d.\})×25 cm, 5 μm\] was employed. Regression equations revealed the linear relationships( r =0 998 and 0 999) between the peak areas of GA measured by ELSD and DAD and its concentration. The recoveries of GA measured by ELSD and DAD are 104 22% and 97 65%, respectively. The contents of GA measured by ELSD and DAD are 13 9 mg/g(2 79% RSD) and 12 1 mg/g(2 99% RSD) respectively in Gastrodia elata Blume . The detection limits of ELSD and DAD( S/N =3) are 3 mg/L and 1 mg/L respectively. This method is simple, selective and sensitive.展开更多
采用高效液相色谱二极管阵列检测法(HPLC-DAD)快速测定桑树(Morus alba L.)上桑叶和桑枝条皮层(以下简称桑枝皮)中的4种色素(叶黄素、β-胡萝卜素、叶绿素a和叶绿素b)含量,分析各种色素含量在不同叶位和不同桑树品种间存在的差异。结果...采用高效液相色谱二极管阵列检测法(HPLC-DAD)快速测定桑树(Morus alba L.)上桑叶和桑枝条皮层(以下简称桑枝皮)中的4种色素(叶黄素、β-胡萝卜素、叶绿素a和叶绿素b)含量,分析各种色素含量在不同叶位和不同桑树品种间存在的差异。结果表明,不同叶位桑叶中的类胡萝卜素含量高于叶绿素含量,但不同叶位桑叶中的上述色素含量具有差异,总体上是成熟叶或老叶中的含量较高,其中β-胡萝卜素、叶绿素b在第7位成熟叶中的含量最高,分别为254.06、117.66μg/g;叶绿素a在第11位成熟叶中的含量最高,为78.77μg/g;叶黄素在第5位衰老叶中的含量最高,为250.66μg/g。不同桑树品种桑叶和桑枝皮中的色素总含量有差异,在10个供试品种中对于桑叶而言含量最高的是湖桑32(1 128.32μg/g);对于桑枝皮而言,沙二含量最高(190.21μg/g)。桑枝皮中色素平均总含量在100μg/g左右,约为桑叶的1/8。所以,从废弃的桑枝条皮层中或从桑枝皮加工废料中提取或回收脂溶性的叶绿素和类胡萝卜素特别是叶黄素是切实可行的。测定方法和试验结果可为今后更好地开发和利用桑树资源特别是桑树枝条皮层中的色素提供重要的试验数据。展开更多
文摘A normal phase high performance chromatographic method with evaporative light scattering detector(ELSD) and diode array detector(DAD), using n hexane/methanol/ethyl acetate(volume ratio 6∶3∶2 ) as the mobile phase, was established for the direct determination of active constituents gastrodin(GA) in the ethanol extracts of Gastrodia elata Blume . An silica gel column Zorbax RX SIL\[4 6 mm (\{i.d.\})×25 cm, 5 μm\] was employed. Regression equations revealed the linear relationships( r =0 998 and 0 999) between the peak areas of GA measured by ELSD and DAD and its concentration. The recoveries of GA measured by ELSD and DAD are 104 22% and 97 65%, respectively. The contents of GA measured by ELSD and DAD are 13 9 mg/g(2 79% RSD) and 12 1 mg/g(2 99% RSD) respectively in Gastrodia elata Blume . The detection limits of ELSD and DAD( S/N =3) are 3 mg/L and 1 mg/L respectively. This method is simple, selective and sensitive.
文摘采用高效液相色谱二极管阵列检测法(HPLC-DAD)快速测定桑树(Morus alba L.)上桑叶和桑枝条皮层(以下简称桑枝皮)中的4种色素(叶黄素、β-胡萝卜素、叶绿素a和叶绿素b)含量,分析各种色素含量在不同叶位和不同桑树品种间存在的差异。结果表明,不同叶位桑叶中的类胡萝卜素含量高于叶绿素含量,但不同叶位桑叶中的上述色素含量具有差异,总体上是成熟叶或老叶中的含量较高,其中β-胡萝卜素、叶绿素b在第7位成熟叶中的含量最高,分别为254.06、117.66μg/g;叶绿素a在第11位成熟叶中的含量最高,为78.77μg/g;叶黄素在第5位衰老叶中的含量最高,为250.66μg/g。不同桑树品种桑叶和桑枝皮中的色素总含量有差异,在10个供试品种中对于桑叶而言含量最高的是湖桑32(1 128.32μg/g);对于桑枝皮而言,沙二含量最高(190.21μg/g)。桑枝皮中色素平均总含量在100μg/g左右,约为桑叶的1/8。所以,从废弃的桑枝条皮层中或从桑枝皮加工废料中提取或回收脂溶性的叶绿素和类胡萝卜素特别是叶黄素是切实可行的。测定方法和试验结果可为今后更好地开发和利用桑树资源特别是桑树枝条皮层中的色素提供重要的试验数据。
