以丙烯酰胺（AM）与二甲基二烯丙基氯化铵（DMDAAC）为单体,MnCl_2/NaHSO_3为引发体系,通过水溶液聚合得到AM/DMDAAC共聚物;利用IR和~1H NMR等方法分析共聚物的结构,考察了反应条件对AM/DMDAAC共聚物性能的影响。表征结果显示,AM/DMDAA...以丙烯酰胺（AM）与二甲基二烯丙基氯化铵（DMDAAC）为单体,MnCl_2/NaHSO_3为引发体系,通过水溶液聚合得到AM/DMDAAC共聚物;利用IR和~1H NMR等方法分析共聚物的结构,考察了反应条件对AM/DMDAAC共聚物性能的影响。表征结果显示,AM/DMDAAC共聚物为目标产物。实验结果表明,合成AM/DMDAAC共聚物适宜的反应条件为：引发温度30℃、反应时间4h、n（AM）∶n（DMDAAC）=5∶1、单体用量25%（w）（基于反应体系质量）、n（MnCl_2）∶n（NaHSO_3）=1∶1、引发剂用量为单体质量的0.1%、引发剂采取固体投料方式。在该反应条件下,AM/DMDAAC共聚物的特性黏数为4.92 d L/g、阳离子度为11.30%、收率为45.66%。将MnCl_2/NaHSO_3引发体系与过硫酸铵复配引发共聚,能进一步提高AM/DMDAAC共聚物的收率、特性黏数和阳离子度。展开更多
In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for produci...In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for producing lipase. It was found that the encapsulation system had no negative effect on the growth and lipase productivity, and the lipase produced inside capsules could diffuse freely to the media outside capsules. The cultivation with the capsules was carried out for 15 batches and the lipase in each batch had almost the same activity. In comparison with free cell cultivation, the immobilized cell fermentation shortened obviously the period of glucose consumption from 24 hours to 8 hours and increased lipase productivity.展开更多
文摘以丙烯酰胺（AM）与二甲基二烯丙基氯化铵（DMDAAC）为单体,MnCl_2/NaHSO_3为引发体系,通过水溶液聚合得到AM/DMDAAC共聚物;利用IR和~1H NMR等方法分析共聚物的结构,考察了反应条件对AM/DMDAAC共聚物性能的影响。表征结果显示,AM/DMDAAC共聚物为目标产物。实验结果表明,合成AM/DMDAAC共聚物适宜的反应条件为：引发温度30℃、反应时间4h、n（AM）∶n（DMDAAC）=5∶1、单体用量25%（w）（基于反应体系质量）、n（MnCl_2）∶n（NaHSO_3）=1∶1、引发剂用量为单体质量的0.1%、引发剂采取固体投料方式。在该反应条件下,AM/DMDAAC共聚物的特性黏数为4.92 d L/g、阳离子度为11.30%、收率为45.66%。将MnCl_2/NaHSO_3引发体系与过硫酸铵复配引发共聚,能进一步提高AM/DMDAAC共聚物的收率、特性黏数和阳离子度。
文摘In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for producing lipase. It was found that the encapsulation system had no negative effect on the growth and lipase productivity, and the lipase produced inside capsules could diffuse freely to the media outside capsules. The cultivation with the capsules was carried out for 15 batches and the lipase in each batch had almost the same activity. In comparison with free cell cultivation, the immobilized cell fermentation shortened obviously the period of glucose consumption from 24 hours to 8 hours and increased lipase productivity.
