Ribavirin production by the mixed fermentation of Bacillus substilis TM903 and Corynebacterium glutamicum TL1105 was studied.The inoculum size of TL1105 and the optimal addition of KH2PO4, MnSO4, 1H-1,2,4-triazole-3-c...Ribavirin production by the mixed fermentation of Bacillus substilis TM903 and Corynebacterium glutamicum TL1105 was studied.The inoculum size of TL1105 and the optimal addition of KH2PO4, MnSO4, 1H-1,2,4-triazole-3-carboxamide (TCA) in the fermentation medium were established initially.Then, the factorial design by MINITAB software was employed to optimize the adding mode of glucose, KH2PO4, MnSO4, TCA, which suggested that MINITAB software was the more effective tool in the culture medium optimization.Under the optimal conditions, the yield of ribavirin was 4.69 g·L-1 after 60 h fermentation with glucose added one-off and KH2PO4, Mn2+and TCA fed in batches.展开更多
三氮唑核苷是医用抗病毒药物,临床上用于治疗急性流行性结膜炎、流行性感冒、口腔疱疹等多种病毒性疾病。其作用机制是抑制病毒合成酶,从而抑制多种 RNA 和 DNA 病毒复制。目前兽医临床上尚无广泛应用的抗病毒类药物治疗病毒性疾病,为...三氮唑核苷是医用抗病毒药物,临床上用于治疗急性流行性结膜炎、流行性感冒、口腔疱疹等多种病毒性疾病。其作用机制是抑制病毒合成酶,从而抑制多种 RNA 和 DNA 病毒复制。目前兽医临床上尚无广泛应用的抗病毒类药物治疗病毒性疾病,为此笔者进行了三氮唑核苷对鸡马立克火鸡疱疹病毒的抑制作用试验,现将结果报告如下。展开更多
文摘Ribavirin production by the mixed fermentation of Bacillus substilis TM903 and Corynebacterium glutamicum TL1105 was studied.The inoculum size of TL1105 and the optimal addition of KH2PO4, MnSO4, 1H-1,2,4-triazole-3-carboxamide (TCA) in the fermentation medium were established initially.Then, the factorial design by MINITAB software was employed to optimize the adding mode of glucose, KH2PO4, MnSO4, TCA, which suggested that MINITAB software was the more effective tool in the culture medium optimization.Under the optimal conditions, the yield of ribavirin was 4.69 g·L-1 after 60 h fermentation with glucose added one-off and KH2PO4, Mn2+and TCA fed in batches.
文摘三氮唑核苷是医用抗病毒药物,临床上用于治疗急性流行性结膜炎、流行性感冒、口腔疱疹等多种病毒性疾病。其作用机制是抑制病毒合成酶,从而抑制多种 RNA 和 DNA 病毒复制。目前兽医临床上尚无广泛应用的抗病毒类药物治疗病毒性疾病,为此笔者进行了三氮唑核苷对鸡马立克火鸡疱疹病毒的抑制作用试验,现将结果报告如下。
