2010年上映的美国电影《怦然心动》讲述了一段清新温暖的成长故事,同年上映的日本电影《告白》讲述的是阴暗惊悚的复仇故事,两部电影风格迥异却广受好评,同样运用了独特的POV(Point Of View,视点人物写作手法)来构建整个故事,在丰富电...2010年上映的美国电影《怦然心动》讲述了一段清新温暖的成长故事,同年上映的日本电影《告白》讲述的是阴暗惊悚的复仇故事,两部电影风格迥异却广受好评,同样运用了独特的POV(Point Of View,视点人物写作手法)来构建整个故事,在丰富电影叙事的同时也增添了影片的艺术性与研究价值。本文分别对《怦然心动》和《告白》的叙事视角及效果进行分析,探索POV手法在不同题材影片中的叙事魅力。展开更多
Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tis- sues and to validate its bona fide targets in human cholangiocarcinoma cells. Methods The expression profile of microRNA...Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tis- sues and to validate its bona fide targets in human cholangiocarcinoma cells. Methods The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholan- giocarcinoma cell line, QBC939, was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot, respec- tively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarci- noma tissues by using real-time PCR, locked nucleic acid in situ hybridization （LNA-ISH）, and immunohis- tochemistry analysis. Results Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bileduct tissues （P〈0.05）. The dual luciferase reporter gene assay revealed endogenous microRNA-21 in cholan- giocarcinoma cell line, QBC939, inhibited the luciferase reporter activities of wild-type PTEN （P〈0.01） and PDCD4 （P〈0.05） and had no this effect on mutated PTEN and PDCD4. Moreover, loss of microRNA-21 function led to a significant increase of PTEN and PDCD4 protein levels in QBC939 cells. Elevated microRNA-21 levels were accompanied by marked reductions of PTEN and PDCD4 expression in the same cholangiocarcinoma tissue. Conclusion microRNA-21 expression is up PDCD4 are direct effectors of microRNA-21. regulated in human cholangiocarcinoma and PTEN,展开更多
TNTESTINAL lymphangiectasia （IL） is a rare disease first reported by Waldmanin in 1961.1 Since then, no more than two hundred cases have been reported. IL is characterized by dilated lymphatic vessles in the intesti...TNTESTINAL lymphangiectasia （IL） is a rare disease first reported by Waldmanin in 1961.1 Since then, no more than two hundred cases have been reported. IL is characterized by dilated lymphatic vessles in the intestinal wall and small bowel mesentery which induceloss of protein and lymphocytes into bowel lumen. We here report a case of IL in a young male patient who was admitted for complaint of recurrent diarrhea for ten years and aggravation of the illness one year ago. He was diagnosed by endoscopy and confirmed by pathology as a primary IL and treated by parenteral nutrition support and Chinese herbs.展开更多
POLYNEUROPATHY, organomegaly, endocrinopathy, monoclonal gammopathy, and skin changes （POEMS） syndrome is a rare paraneoplastic syndrome characterized by the presence of a monoclonal plasma cell disorder, peripheral...POLYNEUROPATHY, organomegaly, endocrinopathy, monoclonal gammopathy, and skin changes （POEMS） syndrome is a rare paraneoplastic syndrome characterized by the presence of a monoclonal plasma cell disorder, peripheral neuropathy, organomegaly, endocrinopathy, monoclonal gammopathy, and typical skin abnormalities. The cause of POEMS syndrome is unknown.1 Although numerous cases with POEMS syndrome have been reported, the POEMS syndrome patients with malignant tumor were seldom reported. Here, we present a rare case with breast cancer accompanied by POEMS syndrome.展开更多
HEMOPHAGOCYTIC lymphohistiocytosis(HLH)is an aggressive and potentially fatal syndrome that results from inappropriate activation of lymphocytes and macrophages.It is characterized by fever,hepatosplenomegaly,cytopeni...HEMOPHAGOCYTIC lymphohistiocytosis(HLH)is an aggressive and potentially fatal syndrome that results from inappropriate activation of lymphocytes and macrophages.It is characterized by fever,hepatosplenomegaly,cytopenias,hypertriglyceridemia,hypofibrinogenemia,and pathologic findings of hemophagocytosis in the bone marrow or other tissues.We report an adult HLH case admitted to hepatology department.展开更多
文摘2010年上映的美国电影《怦然心动》讲述了一段清新温暖的成长故事,同年上映的日本电影《告白》讲述的是阴暗惊悚的复仇故事,两部电影风格迥异却广受好评,同样运用了独特的POV(Point Of View,视点人物写作手法)来构建整个故事,在丰富电影叙事的同时也增添了影片的艺术性与研究价值。本文分别对《怦然心动》和《告白》的叙事视角及效果进行分析,探索POV手法在不同题材影片中的叙事魅力。
基金Supported by Institute of Basic Medical Sciences(2009PY13 and 2010PYZ18)the National Natural Science Foundation of China(81100608 and 30901342)
文摘Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tis- sues and to validate its bona fide targets in human cholangiocarcinoma cells. Methods The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholan- giocarcinoma cell line, QBC939, was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot, respec- tively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarci- noma tissues by using real-time PCR, locked nucleic acid in situ hybridization （LNA-ISH）, and immunohis- tochemistry analysis. Results Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bileduct tissues （P〈0.05）. The dual luciferase reporter gene assay revealed endogenous microRNA-21 in cholan- giocarcinoma cell line, QBC939, inhibited the luciferase reporter activities of wild-type PTEN （P〈0.01） and PDCD4 （P〈0.05） and had no this effect on mutated PTEN and PDCD4. Moreover, loss of microRNA-21 function led to a significant increase of PTEN and PDCD4 protein levels in QBC939 cells. Elevated microRNA-21 levels were accompanied by marked reductions of PTEN and PDCD4 expression in the same cholangiocarcinoma tissue. Conclusion microRNA-21 expression is up PDCD4 are direct effectors of microRNA-21. regulated in human cholangiocarcinoma and PTEN,
基金Supported by the Shanghai Leading Academic Discipline Project(J50305)
文摘TNTESTINAL lymphangiectasia （IL） is a rare disease first reported by Waldmanin in 1961.1 Since then, no more than two hundred cases have been reported. IL is characterized by dilated lymphatic vessles in the intestinal wall and small bowel mesentery which induceloss of protein and lymphocytes into bowel lumen. We here report a case of IL in a young male patient who was admitted for complaint of recurrent diarrhea for ten years and aggravation of the illness one year ago. He was diagnosed by endoscopy and confirmed by pathology as a primary IL and treated by parenteral nutrition support and Chinese herbs.
基金Supported by a grant from the Science and Technology Planning Project of Guangdong Province,China(2013B010404023)
文摘POLYNEUROPATHY, organomegaly, endocrinopathy, monoclonal gammopathy, and skin changes （POEMS） syndrome is a rare paraneoplastic syndrome characterized by the presence of a monoclonal plasma cell disorder, peripheral neuropathy, organomegaly, endocrinopathy, monoclonal gammopathy, and typical skin abnormalities. The cause of POEMS syndrome is unknown.1 Although numerous cases with POEMS syndrome have been reported, the POEMS syndrome patients with malignant tumor were seldom reported. Here, we present a rare case with breast cancer accompanied by POEMS syndrome.
文摘HEMOPHAGOCYTIC lymphohistiocytosis(HLH)is an aggressive and potentially fatal syndrome that results from inappropriate activation of lymphocytes and macrophages.It is characterized by fever,hepatosplenomegaly,cytopenias,hypertriglyceridemia,hypofibrinogenemia,and pathologic findings of hemophagocytosis in the bone marrow or other tissues.We report an adult HLH case admitted to hepatology department.
