The method of simultaneous analysis of Sudan Red Ⅰ-Ⅳ residues in ketchup was developed with an ultra performance liquid chromatography-tandem mass spectrum.Acetonitrile was used to extract the substance from the ket...The method of simultaneous analysis of Sudan Red Ⅰ-Ⅳ residues in ketchup was developed with an ultra performance liquid chromatography-tandem mass spectrum.Acetonitrile was used to extract the substance from the ketchup,and the upper layer was detected by the UPLC-MS/MS after refrigeration and centrifuge.The sample was separated on a Waters Acquity BHE C18 column,and detected by the MRM mode of the mass spectrum.The LOD was 2 ng/mL and the LOQ was 5 ng/mL all of the four substances.The average recoveries were between 67.42%-84.59% of the 100,200,300 μg/kg three spiked concentration levels and the RSD values were between 2.87%-9.57%.展开更多
文摘The method of simultaneous analysis of Sudan Red Ⅰ-Ⅳ residues in ketchup was developed with an ultra performance liquid chromatography-tandem mass spectrum.Acetonitrile was used to extract the substance from the ketchup,and the upper layer was detected by the UPLC-MS/MS after refrigeration and centrifuge.The sample was separated on a Waters Acquity BHE C18 column,and detected by the MRM mode of the mass spectrum.The LOD was 2 ng/mL and the LOQ was 5 ng/mL all of the four substances.The average recoveries were between 67.42%-84.59% of the 100,200,300 μg/kg three spiked concentration levels and the RSD values were between 2.87%-9.57%.
