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丽江大研古城文化变迁中的“虚无”与“实在”:以酒吧发展为例 被引量:49
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作者 孙九霞 王心蕊 《旅游学刊》 CSSCI 2012年第9期73-83,共11页
全球化背景下旅游业迅速发展,旅游目的地文化变迁日益显著,并引起人们的关注。作为世界文化遗产的丽江大研古城自旅游开发至今吸引了来自世界各地的大量游客,古城原生文化也经历着某种程度的变迁。文章在两次实地调研的基础上,综合运用... 全球化背景下旅游业迅速发展,旅游目的地文化变迁日益显著,并引起人们的关注。作为世界文化遗产的丽江大研古城自旅游开发至今吸引了来自世界各地的大量游客,古城原生文化也经历着某种程度的变迁。文章在两次实地调研的基础上,综合运用访谈法和观察法,以古城酒吧发展的视角,探求反映古城文化变迁过程,并应用乔治.里茨尔"虚无"与"实在"理论分析古城酒吧发展过程中"虚无"与"实在"的表征及动因。 展开更多
关键词 丽江古城 酒吧 原生文化 小资文化 “虚无”与“实在”
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胸膜肺炎放线杆菌 tadF 基因缺失株构建及生物学特性研究
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作者 熊威振 冀笑明 +4 位作者 任豆豆 梁萱 魏雨婷 崔国林 许金朋 《动物医学进展》 北大核心 2025年第2期10-16,共7页
为探究tadF基因对胸膜肺炎放线杆菌(App)生物学特性及毒力的影响,研究基于App血清1型4074株,通过两步单交换同源重组及正负向筛选,构建tadF基因缺失株,并测定亲本株和缺失株生长曲线、侧翼基因表达水平、生物被膜形成能力、细胞黏附入... 为探究tadF基因对胸膜肺炎放线杆菌(App)生物学特性及毒力的影响,研究基于App血清1型4074株,通过两步单交换同源重组及正负向筛选,构建tadF基因缺失株,并测定亲本株和缺失株生长曲线、侧翼基因表达水平、生物被膜形成能力、细胞黏附入侵能力和对小鼠的致病力。结果显示,成功构建tadF基因缺失株4074ΔtadF,该基因缺失对App生物被膜形成能力和侧翼基因表达水平无显著影响,但缺失株增殖速度慢于亲本株,tadF基因缺失显著降低App对PAM细胞的黏附入侵能力(P<0.05),亲本株黏附入侵能力为缺失株的1.75倍;对小鼠致病力检测结果显示,缺失株对小鼠的LD 50是亲本株的2.5倍,表明tadF基因缺失株对小鼠致病力下降。说明胸膜肺炎放线杆菌tadF基因缺失导致App增殖变慢,并降低其对细胞黏附入侵能力和对小鼠的毒力,为App黏附素作用机制研究提供参考。 展开更多
关键词 胸膜肺炎放线杆菌 tadF基因 黏附素 缺失株 生物学特性
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Research and application of an intelligent recloser controller installed on outdoor rod
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作者 廖力清 陈燕辉 +1 位作者 凌玉华 杨欣荣 《Journal of Central South University of Technology》 2002年第1期59-63,共5页
A new type of intelligent recolser controller installed on the outdoor rod is developed, which is mainly composed of microcontroller of Intel 87C196KC 20 and CPLD devices. This controller integrates all the functions ... A new type of intelligent recolser controller installed on the outdoor rod is developed, which is mainly composed of microcontroller of Intel 87C196KC 20 and CPLD devices. This controller integrates all the functions of measuring, controlling, protection, fault diagnosis, communication, remote controlled operation and self power devices with infra red remote control devices as a unit. The controller applies the distributed structure, field concentration line and intelligent technology to seal up the synthetic servomechanisms such as the microcomputer based protection and measuring devices in the second stage of the mini out door transformer substation, which are distributed on the outdoor circuit switches on the spot and formed as a whole. Therefore, this technology can transform a large number of ordinary homemade SF 6 circuit beaker and vacuum circuit breaker into intelligent circuit recloser, thus replacing the expensive imported automatic circuit recolser. 展开更多
关键词 80C196KC intelligent recloser controller alternating current sampling on line real time detection
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Effective model based fault detection scheme for rudder servo system
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作者 徐巧宁 周华 +2 位作者 喻峰 魏兴乔 杨华勇 《Journal of Central South University》 SCIE EI CAS 2014年第11期4172-4183,共12页
The inherent nonlinearities of the rudder servo system(RSS) and the unknown external disturbances bring great challenges to the practical application of fault detection technology. Modeling of whole rudder system is a... The inherent nonlinearities of the rudder servo system(RSS) and the unknown external disturbances bring great challenges to the practical application of fault detection technology. Modeling of whole rudder system is a challenging and difficult task. Quite often, models are too inaccurate, especially in transient stages. In model based fault detection, these inaccuracies might cause wrong actions. An effective approach, which combines nonlinear unknown input observer(NUIO) with an adaptive threshold, is proposed. NUIO can estimate the states of RSS asymptotically without any knowledge of external disturbance. An adaptive threshold is used for decision making which helps to reduce the influence of model uncertainty. Actuator and sensor faults that occur in RSS are considered both by simulation and experimental tests. The observer performance, robustness and fault detection capability are verified. Simulation and experimental results show that the proposed fault detection scheme is efficient and can be used for on-line fault detection. 展开更多
关键词 rudder servo system fault detection nonlinear unknown input observer adaptive threshold
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