Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain unclear.The aim of this study was to rapidly screen for such pept...Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain unclear.The aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis.In total,1262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were identified.Enzyme kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive inhibitor.This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site.These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.展开更多
The Manchurian walnut(Juglans mandshurica Maxim.) is rich in proteins, whereas this resource has not been used efficiently. The antifatigue, antioxidative and immunoregulatory effects of Manchurian walnut hydrolysate ...The Manchurian walnut(Juglans mandshurica Maxim.) is rich in proteins, whereas this resource has not been used efficiently. The antifatigue, antioxidative and immunoregulatory effects of Manchurian walnut hydrolysate peptides(MWHPs)were evaluated in this study. MWHPs with a degree of hydrolysis of 32.23% were ultrafiltered and divided into three fractions,namely, high(> 10 k Da), medium(3–10 kDa), and low molecular weight(< 3 kDa), and then fed to mice continuously at doses of 200, 400 or 800 mg/(kg·d). The antifatigue, antioxidative, and immunoregulatory effects of the peptides were tested on the second and fourth weeks of MWHP administration. Results showed that low-molecular-weight MWHPs exerted significant antifatigue(prolonging swimming time, elevating liver glycogen contents, and reducing lactic acid contents), antioxidative(enhancing superoxide dismutase(SOD), GSH-Px, and catalase(CAT) activities and reducing malondialdehyde(MDA) content), and immunoregulatory(raising the immune-organ index and promoting T-lymphocyte proliferation and s Ig A secretion in the intestinal tract) effects. This research indicates that MWHPs have potential applications in health care and may be developed as a base for new functional foods.展开更多
Walnut(Juglans regia L.)is a good source of lipids and polyunsaturated fatty acids(PUFAs).In order to explore the biosynthesis molecular mechanism of oil accumulation and fatty acid(FA)synthesis in walnut,the samples ...Walnut(Juglans regia L.)is a good source of lipids and polyunsaturated fatty acids(PUFAs).In order to explore the biosynthesis molecular mechanism of oil accumulation and fatty acid(FA)synthesis in walnut,the samples at different development periods of three walnut cultivars,’Zhipi’(ZP),’Xinwu 417’(W417)and’Xinwen 81’(W81)were collected for transcriptomic analysis.The analysis of oil accumulation and FA profiles showed that the oil content in mature walnut kernel was nearly 70%,and over 90%of FAs were PUFAs.We identified 126 candidate genes including 64 genes for FA de novo synthesis,45 genes for triacylglycerol assembly,and 17 genes for oil bodies involved in lipid biosynthesis by RNA-sequencing.Ten key enzymes including ACCase,LACS6,LACS8,SAD,FAD2,FAD3,LPAAT1,DGAT2,PDAT2,and PLC encoded by 19 genes were highly associated with lipid biosynthesis.Quantitative PCR analysis further validated 9 important genes,and the results were well consistent with our transcriptomic data.Finally,5 important transcription factors including WRI1,ABI3,FUS3,PKL and VAL1 were identified,and their main regulatory genes might contain ACCase,KASII,LACS,FAD3 and LPAAT which were determined through correlation analysis of expression levels for 27 walnut samples.These findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in walnut.展开更多
In our previous study, defatted walnut meal hydrolysate(DWMH) could attenuate D-galactose-induced acute memory deficits in vivo, and six potent active peptides including WSREEQ, WSREEQE, WSREEQEREE, ADIYTE, ADIYTEEAG ...In our previous study, defatted walnut meal hydrolysate(DWMH) could attenuate D-galactose-induced acute memory deficits in vivo, and six potent active peptides including WSREEQ, WSREEQE, WSREEQEREE, ADIYTE, ADIYTEEAG and ADIYTEEAGR were identified. The aim of this study was to investigate the possible mechanism underlying their neuroprotective effects on glutamate-induced apoptosis in PC12 cells and their digestive stability. Results showed that all these peptides could attenuate the reduction of cell viability caused by glutamate in PC12 cells, especially WSREEQEREE and ADIYTEEAGR. The addition of Arg residue in WSREEQEREE and ADIYTEEAGR might be the potential reason for their stronger protective effects. Additionally, these two peptides possibly protected PC12 cells against glutamate-induced apoptosis via activating intracellular antioxidant defence(superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px)) through Kelch-like ECH-associated protein 1(Keap1) inhibition, inhibiting ROS production, Ca;influx and mitochondrial membrane potential(MMP) collapse as well as regulating the expression of apoptosis-related proteins(Bax and Bcl-2). This might be due to the presence of Trp, Tyr and Arg in these two peptides. However, encapsulation of WSREEQEREE and ADIYTEEAGR should be considered based on their digestive sensibility during in vitro gastrointestinal digestion.展开更多
According to the study on soils under the 34-year-old Larch forest and Larch-Walnut mixed forest. It tvas concluded that, in mixed forest, the total content of water- stable aggregate (0. 25- 5mm) in the upper layers ...According to the study on soils under the 34-year-old Larch forest and Larch-Walnut mixed forest. It tvas concluded that, in mixed forest, the total content of water- stable aggregate (0. 25- 5mm) in the upper layers (0- 30cm) of soil was significant ly higher than that in pure forest. The 2mm aggregates increased 48% in mixed forest soil compared with pure fOrest. and consequently, the three-phase ratio of soil was regulate d, the physical properties improved and the fertility of soil raised. Because of the stability of total aggregate content and the importance of 2mm aggregate content in soil fertility.it s advisable using the two indices above to characterize the effect of mixed forest on soil improvement.展开更多
基金supported by the Major Project of Science and Technology Department of Yunnan Province (202002AA100005 and 202102AE090027-2)the Project of Yunnan Province Food and Drug Homologous Resources Functional Food Innovation Team (A3032023057)+2 种基金the YEFICRC project of Yunnan provincial key programs (2019ZG009)Yunnan Province Ten Thousand Plan Industrial Technology Talents project (YNWR-CYJS-2020-010)the Yunnan Provincial Department of Science and Technology Agricultural Joint Special Project (202101BD070001-120)。
文摘Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain unclear.The aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis.In total,1262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were identified.Enzyme kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive inhibitor.This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site.These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.
基金Supported by National High Technology Research and Development Program(No.2013AA102206-2)
文摘The Manchurian walnut(Juglans mandshurica Maxim.) is rich in proteins, whereas this resource has not been used efficiently. The antifatigue, antioxidative and immunoregulatory effects of Manchurian walnut hydrolysate peptides(MWHPs)were evaluated in this study. MWHPs with a degree of hydrolysis of 32.23% were ultrafiltered and divided into three fractions,namely, high(> 10 k Da), medium(3–10 kDa), and low molecular weight(< 3 kDa), and then fed to mice continuously at doses of 200, 400 or 800 mg/(kg·d). The antifatigue, antioxidative, and immunoregulatory effects of the peptides were tested on the second and fourth weeks of MWHP administration. Results showed that low-molecular-weight MWHPs exerted significant antifatigue(prolonging swimming time, elevating liver glycogen contents, and reducing lactic acid contents), antioxidative(enhancing superoxide dismutase(SOD), GSH-Px, and catalase(CAT) activities and reducing malondialdehyde(MDA) content), and immunoregulatory(raising the immune-organ index and promoting T-lymphocyte proliferation and s Ig A secretion in the intestinal tract) effects. This research indicates that MWHPs have potential applications in health care and may be developed as a base for new functional foods.
基金supported by Major scientific and technological projects of Xinjiang Production and Construction Corps(2017DB006 and 2020KWZ-012)。
文摘Walnut(Juglans regia L.)is a good source of lipids and polyunsaturated fatty acids(PUFAs).In order to explore the biosynthesis molecular mechanism of oil accumulation and fatty acid(FA)synthesis in walnut,the samples at different development periods of three walnut cultivars,’Zhipi’(ZP),’Xinwu 417’(W417)and’Xinwen 81’(W81)were collected for transcriptomic analysis.The analysis of oil accumulation and FA profiles showed that the oil content in mature walnut kernel was nearly 70%,and over 90%of FAs were PUFAs.We identified 126 candidate genes including 64 genes for FA de novo synthesis,45 genes for triacylglycerol assembly,and 17 genes for oil bodies involved in lipid biosynthesis by RNA-sequencing.Ten key enzymes including ACCase,LACS6,LACS8,SAD,FAD2,FAD3,LPAAT1,DGAT2,PDAT2,and PLC encoded by 19 genes were highly associated with lipid biosynthesis.Quantitative PCR analysis further validated 9 important genes,and the results were well consistent with our transcriptomic data.Finally,5 important transcription factors including WRI1,ABI3,FUS3,PKL and VAL1 were identified,and their main regulatory genes might contain ACCase,KASII,LACS,FAD3 and LPAAT which were determined through correlation analysis of expression levels for 27 walnut samples.These findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in walnut.
基金supported by the Taishan Industry Leading Talent Project, Guangdong Provincial Key R&D Program (2020B020226005)the Specific Fund Program for Basic and Applied Basic Research of Guangdong Province (2019A1515011952)+2 种基金the Fundamental Research Funds for the Central Universities (No. x2skD2192510)the Natural Science Foundation of Guangdong for Basic and Applied Basic Research (2020A1515010659)Special Support Project of Guangxi Province for Innovation driven Development (Guangdong Huapeptides Biotechnology Co., Ltd., AA17204075)。
文摘In our previous study, defatted walnut meal hydrolysate(DWMH) could attenuate D-galactose-induced acute memory deficits in vivo, and six potent active peptides including WSREEQ, WSREEQE, WSREEQEREE, ADIYTE, ADIYTEEAG and ADIYTEEAGR were identified. The aim of this study was to investigate the possible mechanism underlying their neuroprotective effects on glutamate-induced apoptosis in PC12 cells and their digestive stability. Results showed that all these peptides could attenuate the reduction of cell viability caused by glutamate in PC12 cells, especially WSREEQEREE and ADIYTEEAGR. The addition of Arg residue in WSREEQEREE and ADIYTEEAGR might be the potential reason for their stronger protective effects. Additionally, these two peptides possibly protected PC12 cells against glutamate-induced apoptosis via activating intracellular antioxidant defence(superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px)) through Kelch-like ECH-associated protein 1(Keap1) inhibition, inhibiting ROS production, Ca;influx and mitochondrial membrane potential(MMP) collapse as well as regulating the expression of apoptosis-related proteins(Bax and Bcl-2). This might be due to the presence of Trp, Tyr and Arg in these two peptides. However, encapsulation of WSREEQEREE and ADIYTEEAGR should be considered based on their digestive sensibility during in vitro gastrointestinal digestion.
文摘According to the study on soils under the 34-year-old Larch forest and Larch-Walnut mixed forest. It tvas concluded that, in mixed forest, the total content of water- stable aggregate (0. 25- 5mm) in the upper layers (0- 30cm) of soil was significant ly higher than that in pure forest. The 2mm aggregates increased 48% in mixed forest soil compared with pure fOrest. and consequently, the three-phase ratio of soil was regulate d, the physical properties improved and the fertility of soil raised. Because of the stability of total aggregate content and the importance of 2mm aggregate content in soil fertility.it s advisable using the two indices above to characterize the effect of mixed forest on soil improvement.
