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Vibration reliability analysis for aeroengine compressor blade based on support vector machine response surface method
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作者 高海峰 白广忱 《Journal of Central South University》 SCIE EI CAS CSCD 2015年第5期1685-1694,共10页
To ameliorate reliability analysis efficiency for aeroengine components, such as compressor blade, support vector machine response surface method(SRSM) is proposed. SRSM integrates the advantages of support vector mac... To ameliorate reliability analysis efficiency for aeroengine components, such as compressor blade, support vector machine response surface method(SRSM) is proposed. SRSM integrates the advantages of support vector machine(SVM) and traditional response surface method(RSM), and utilizes experimental samples to construct a suitable response surface function(RSF) to replace the complicated and abstract finite element model. Moreover, the randomness of material parameters, structural dimension and operating condition are considered during extracting data so that the response surface function is more agreeable to the practical model. The results indicate that based on the same experimental data, SRSM has come closer than RSM reliability to approximating Monte Carlo method(MCM); while SRSM(17.296 s) needs far less running time than MCM(10958 s) and RSM(9840 s). Therefore,under the same simulation conditions, SRSM has the largest analysis efficiency, and can be considered a feasible and valid method to analyze structural reliability. 展开更多
关键词 VIBRATION reliability analysis compressor blade support vector machine response surface method natural frequency
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Graphlet Degree Vector方法的优化与并行
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作者 宋祥帅 杨伏长 +1 位作者 谢江 张武 《计算机应用》 CSCD 北大核心 2020年第2期398-403,共6页
Graphlet Degree Vector(GDV)是一种研究生物网络的重要方法,能揭示生物网络中各节点与其局部网络结构的相关性,但随着需要挖掘的自同构轨道数量的增加以及生物网络规模的增大,GDV方法的时间复杂度会呈指数级增长。针对这个问题,在现有... Graphlet Degree Vector(GDV)是一种研究生物网络的重要方法,能揭示生物网络中各节点与其局部网络结构的相关性,但随着需要挖掘的自同构轨道数量的增加以及生物网络规模的增大,GDV方法的时间复杂度会呈指数级增长。针对这个问题,在现有串行GDV方法的基础上,实现了基于消息传递接口(MPI)的GDV方法并行化;此外又将GDV方法进行了改进并将改进后的方法实现了并行优化,改进后的方法在寻找不同节点自同构轨道的过程中优化了计算过程以解决重复计算的问题,同时结合负载均衡策略合理分配任务。模拟网络数据和真实生物网络数据上的实验结果表明,并行化的GDV方法与改进后的并行化GDV方法都具有较好的并行性能,并且对不同类型不同规模的网络都具有较强的适用性,扩展性强,可有效地保持寻找网络中自同构轨道的高效率。 展开更多
关键词 Graphlet DEGREE vector方法 生物网络 自同构轨道 子图枚举 并行化 消息传递接口
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Probabilistic back analysis for geotechnical engineering based on Bayesian and support vector machine 被引量:2
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作者 陈炳瑞 赵洪波 +1 位作者 茹忠亮 李贤 《Journal of Central South University》 SCIE EI CAS CSCD 2015年第12期4778-4786,共9页
Geomechanical parameters are complex and uncertain.In order to take this complexity and uncertainty into account,a probabilistic back-analysis method combining the Bayesian probability with the least squares support v... Geomechanical parameters are complex and uncertain.In order to take this complexity and uncertainty into account,a probabilistic back-analysis method combining the Bayesian probability with the least squares support vector machine(LS-SVM) technique was proposed.The Bayesian probability was used to deal with the uncertainties in the geomechanical parameters,and an LS-SVM was utilized to establish the relationship between the displacement and the geomechanical parameters.The proposed approach was applied to the geomechanical parameter identification in a slope stability case study which was related to the permanent ship lock within the Three Gorges project in China.The results indicate that the proposed method presents the uncertainties in the geomechanical parameters reasonably well,and also improves the understanding that the monitored information is important in real projects. 展开更多
关键词 geotechnical engineering back analysis UNCERTAINTY Bayesian theory least square method support vector machine(SVM)
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Developing a Support Vector Machine Based QSPR Model to Predict Gas-to-Benzene Solvation Enthalpy of Organic Compounds 被引量:1
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作者 GOLMOHAMMADI Hassan DASHTBOZORGI Zahra KHOOSHECHIN Sajad 《物理化学学报》 SCIE CAS CSCD 北大核心 2017年第5期918-926,共9页
The purpose of this paper is to present a novel way to building quantitative structure-property relationship(QSPR) models for predicting the gas-to-benzene solvation enthalpy(ΔHSolv) of 158 organic compounds based on... The purpose of this paper is to present a novel way to building quantitative structure-property relationship(QSPR) models for predicting the gas-to-benzene solvation enthalpy(ΔHSolv) of 158 organic compounds based on molecular descriptors calculated from the structure alone. Different kinds of descriptors were calculated for each compounds using dragon package. The variable selection technique of enhanced replacement method(ERM) was employed to select optimal subset of descriptors. Our investigation reveals that the dependence of physico-chemical properties on solvation enthalpy is a nonlinear observable fact and that ERM method is unable to model the solvation enthalpy accurately. The standard error value of prediction set for support vector machine(SVM) is 1.681 kJ ? mol^(-1) while it is 4.624 kJ ? mol^(-1) for ERM. The results established that the calculated ΔHSolvvalues by SVM were in good agreement with the experimental ones, and the performances of the SVM models were superior to those obtained by ERM one. This indicates that SVM can be used as an alternative modeling tool for QSPR studies. 展开更多
关键词 数量的结构-财产关系 气体-到-苯媒合焓 描述符 提高了复位成本折旧法 支承矢量机器
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Compression method based on training dataset of SVM
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作者 Ban Xiaojuan Shen Qilong +1 位作者 Chen Hao Tu Xuyan 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2008年第1期198-201,共4页
The method to compress the training dataset of Support Vector Machine (SVM) based on the character of the Support Vector Machine is proposed. First, the distance between the unit in two training datasets, and then t... The method to compress the training dataset of Support Vector Machine (SVM) based on the character of the Support Vector Machine is proposed. First, the distance between the unit in two training datasets, and then the samples that keep away from hyper-plane are discarded in order to compress the training dataset. The time spent in training SVM with the training dataset compressed by the method is shortened obviously. The result of the experiment shows that the algorithm is effective. 展开更多
关键词 statistical learning theory support vector machine compression method CLASSIFICATION
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A Novel Kernel for Least Squares Support Vector Machine
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作者 冯伟 赵永平 +2 位作者 杜忠华 李德才 王立峰 《Defence Technology(防务技术)》 SCIE EI CAS 2012年第4期240-247,共8页
Extreme learning machine(ELM) has attracted much attention in recent years due to its fast convergence and good performance.Merging both ELM and support vector machine is an important trend,thus yielding an ELM kernel... Extreme learning machine(ELM) has attracted much attention in recent years due to its fast convergence and good performance.Merging both ELM and support vector machine is an important trend,thus yielding an ELM kernel.ELM kernel based methods are able to solve the nonlinear problems by inducing an explicit mapping compared with the commonly-used kernels such as Gaussian kernel.In this paper,the ELM kernel is extended to the least squares support vector regression(LSSVR),so ELM-LSSVR was proposed.ELM-LSSVR can be used to reduce the training and test time simultaneously without extra techniques such as sequential minimal optimization and pruning mechanism.Moreover,the memory space for the training and test was relieved.To confirm the efficacy and feasibility of the proposed ELM-LSSVR,the experiments are reported to demonstrate that ELM-LSSVR takes the advantage of training and test time with comparable accuracy to other algorithms. 展开更多
关键词 计算技术 理论 方法 自动机理论
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A Modeling Method for Transformer Windings Under VFTO Based on S-parameters
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作者 ZHANG Ping WANG You-hua +2 位作者 NIE Xin peng YAN Wei-li ZHANG Hai-jiao 《高电压技术》 EI CAS CSCD 北大核心 2008年第9期1898-1904,共7页
To study the Very Fast Transient Over-voltage (VFTO) distribution in transformer windings in gas insulated substation (GIS), a systematic methodology based on S-parameters is presented for establishing high-frequency ... To study the Very Fast Transient Over-voltage (VFTO) distribution in transformer windings in gas insulated substation (GIS), a systematic methodology based on S-parameters is presented for establishing high-frequency model of transformer windings. Firstly, voltage transfer functions are derived from S-parameters which are calculated or measured from transformer windings. Secondly, voltage transfer functions are fitted with rational functions by the vector fitting method and then the rational transfer functions are order-reduced by optimal Pade-approximation algorithm. Lastly, the resultant voltage transfer functions are synthesized by network technology. Computational results are consistent with simulation results of Electromagnetic Transient Program (EMTP) and confirm the feasibility and validity of proposed methodology. 展开更多
关键词 过电压 瞬变电流 变压器绕组 电压转移法
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Study and application of time series forecasting based on rough set and Kernel method
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作者 杨淑霞 《Journal of Central South University》 SCIE EI CAS 2008年第S2期336-340,共5页
A support vector machine time series forecasting model based on rough set data preprocessing was proposed by combining rough set attribute reduction and support vector machine regression algorithm. First, remove the r... A support vector machine time series forecasting model based on rough set data preprocessing was proposed by combining rough set attribute reduction and support vector machine regression algorithm. First, remove the redundant attribute for forecasting from condition attribute by rough set method; then use the minimum condition attribute set obtained after the reduction and the corresponding initial data, reform a new training sample set which only retain the important attributes influencing the forecasting accuracy; study and train the support vector machine with the training sample obtained after reduction, and then input the reformed testing sample set according to the minimum condition attribute and corresponding initial data. The model was tested and the mapping relation was got between the condition attribute and forecasting variable. Eventually, power supply and demand were forecasted in this model. The average absolute error rates of power consumption of the whole society and yearly maximum load are respectively 14.21% and 13.23%. It shows that RS-SVM time series forecasting model has high forecasting accuracy. 展开更多
关键词 KERNEL method support vector MACHINE ROUGH SET forecasting
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Numerical Analysis of 3mm Ferrite Dielectric Waveguide Through Galerkin’s Method
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作者 刘明宇 张莹 张江华 《Defence Technology(防务技术)》 SCIE EI CAS 2008年第4期272-277,共6页
With the development of long range missile early warning radar,MMW phased array antenna is strongly needed. The phase shifter (PS) with high power capability and low insertion loss is a key technology in this kind of ... With the development of long range missile early warning radar,MMW phased array antenna is strongly needed. The phase shifter (PS) with high power capability and low insertion loss is a key technology in this kind of radar. Ferrite dielectric waveguide is introduced in this paper to manufacture PS,the cross section can be enlarged to 4 times of that of the conventional PS,which facilitates the engineering realization with reduced insertion loss. A novel vector base function of Galerkin method is proposed in calculating the eigenvalue of ferrite dielectric waveguide. The field distribution of main mode,power density and efficiency of 94 GHz difference phase shifter are also given. 展开更多
关键词 雷达工程 波导 电介质 无线电导引
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基于三点矢量法的转角焊缝焊接轨迹规划
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作者 陈琳 陈垚 +2 位作者 李松莛 付钰 潘海鸿 《组合机床与自动化加工技术》 北大核心 2025年第1期94-98,共5页
为解决带转角焊缝焊接中机器人示教步骤复杂问题,提出一种基于三点矢量法的转角焊缝焊接轨迹规划方法。首先,根据示教起点、转角顶点、终点和用户输入的圆弧半径,利用空间矢量法将焊缝分为五段,并修正圆弧半径,进而规划各段焊缝焊接插... 为解决带转角焊缝焊接中机器人示教步骤复杂问题,提出一种基于三点矢量法的转角焊缝焊接轨迹规划方法。首先,根据示教起点、转角顶点、终点和用户输入的圆弧半径,利用空间矢量法将焊缝分为五段,并修正圆弧半径,进而规划各段焊缝焊接插补路径;其次,根据焊接工艺要求,采用旋转矢量法规划焊枪姿态。在处理可能的内侧转角焊缝空间狭窄问题时,应用极限碰撞理论获取焊枪安全角度,以进一步调整焊枪姿态;最后,结合焊接路径规划和焊枪姿态规划,得到完整焊接轨迹。仿真和实验结果表明,该算法简化了机器人示教操作,提高了焊接效率,实现了安全焊接。实践证明,该算法适用于实际焊接工作。 展开更多
关键词 转角焊缝焊接 三点矢量法 旋转矢量法 焊接路径规划 焊枪姿态规划
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Solving large-scale multiclass learning problems via an efficient support vector classifier 被引量:1
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作者 Zheng Shuibo Tang Houjun +1 位作者 Han Zhengzhi Zhang Haoran 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2006年第4期910-915,共6页
Support vector machines (SVMs) are initially designed for binary classification. How to effectively extend them for multiclass classification is still an ongoing research topic. A multiclass classifier is constructe... Support vector machines (SVMs) are initially designed for binary classification. How to effectively extend them for multiclass classification is still an ongoing research topic. A multiclass classifier is constructed by combining SVM^light algorithm with directed acyclic graph SVM (DAGSVM) method, named DAGSVM^light A new method is proposed to select the working set which is identical to the working set selected by SVM^light approach. Experimental results indicate DAGSVM^light is competitive with DAGSMO. It is more suitable for practice use. It may be an especially useful tool for large-scale multiclass classification problems and lead to more widespread use of SVMs in the engineering community due to its good performance. 展开更多
关键词 support vector machines (SVMs) multiclass classification decomposition method SVM^light sequential minimal optimization (SMO).
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基于改进麻雀搜索算法的AUV路径规划方法 被引量:1
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作者 唐李军 范云霞 +1 位作者 周星宇 孙骞 《中国舰船研究》 北大核心 2025年第3期275-287,共13页
[目的]针对复杂水下环境中自主水下航行器(AUV)三维路径规划算法存在的规划效果不理想、路径搜索不稳定等问题,提出一种基于改进麻雀搜索算法的AUV路径规划方法。[方法]推导评价区间响应的矢量分析方法公式,引入分段学习和量子计算机制... [目的]针对复杂水下环境中自主水下航行器(AUV)三维路径规划算法存在的规划效果不理想、路径搜索不稳定等问题,提出一种基于改进麻雀搜索算法的AUV路径规划方法。[方法]推导评价区间响应的矢量分析方法公式,引入分段学习和量子计算机制,改进经典麻雀搜索算法的更新公式。通过汤普森采样策略动态更新种群数量。在复杂洋流环境中进行仿真测试,验证改进算法的有效性。[结果]测试结果表明,改进算法的平均最长航行时间较改进前缩短49.88%,在极端突变的洋流环境下,路径规划失败率降低10.6%。[结论]研究成果揭示了该方法具有较强的全局搜索能力和寻优性能、算法收敛性能较好,具备高效的路径规划能力,对AUV以及其他领域的路径规划问题有借鉴意义。 展开更多
关键词 自主水下航行器 三维路径规划 麻雀搜索算法 区间优化 矢量分析法 运动规划
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块体理论研究进展
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作者 张洪 安宝宝 +1 位作者 郑路 武威 《同济大学学报(自然科学版)》 北大核心 2025年第6期898-905,共8页
侧重总结块体理论与方法研究的历史脉络、发展现状及未来趋势:核心概念从经典锥、形心滑动锥、广义节理锥发展为接触理论的总进入角;配套形成全赤平投影、向量运算、赤平解析、全域切割与接触计算等方法;保留刚体平移失稳基本假设,弱化... 侧重总结块体理论与方法研究的历史脉络、发展现状及未来趋势:核心概念从经典锥、形心滑动锥、广义节理锥发展为接触理论的总进入角;配套形成全赤平投影、向量运算、赤平解析、全域切割与接触计算等方法;保留刚体平移失稳基本假设,弱化无限延伸平直结构面和面-面接触几何约束两大基本假设;将面-面接触几何约束条件下临空凸块体稳定分析推广为一般接触几何约束条件下全域任意形态块体渐进失稳分析。此外,提出总进入角接触理论与计算方法用于块体可动性分析,以期进一步推动块体理论实质性发展。 展开更多
关键词 块体理论 接触理论 总进入角 全赤平投影 向量运算 赤平解析
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On Eigen-Matrix Translation Method for Classification of Biological Data
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作者 JIANG Hao QIU Yushan +1 位作者 CHENG Xiaoqing CHING Waiki 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2015年第5期1212-1230,共19页
Driven by the challenge of integrating large amount of experimental data, classification technique emerges as one of the major and popular tools in computational biology and bioinformatics research. Machine learning m... Driven by the challenge of integrating large amount of experimental data, classification technique emerges as one of the major and popular tools in computational biology and bioinformatics research. Machine learning methods, especially kernel methods with Support Vector Machines (SVMs) are very popular and effective tools. In the perspective of kernel matrix, a technique namely Eigen- matrix translation has been introduced for protein data classification. The Eigen-matrix translation strategy has a lot of nice properties which deserve more exploration. This paper investigates the major role of Eigen-matrix translation in classification. The authors propose that its importance lies in the dimension reduction of predictor attributes within the data set. This is very important when the dimension of features is huge. The authors show by numerical experiments on real biological data sets that the proposed framework is crucial and effective in improving classification accuracy. This can therefore serve as a novel perspective for future research in dimension reduction problems. 展开更多
关键词 CLASSIFICATION dimension reduction eigen-matrix translation glycan data kernel method(KM) support vector machine (SVM)
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考虑乘客自主选择的区段交替响应式城乡公交调度
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作者 姜晓红 李家伟 +2 位作者 华晶雯 仲韫豪 邢吉平 《交通运输系统工程与信息》 北大核心 2025年第2期201-213,共13页
为有效应对农村地区公交服务覆盖率低导致居民出行不便的问题,增强乘客作为出行主体的选择权,本文提出一种利用现有城乡公交固定线路资源,考虑乘客自主选择的区段交替响应式调度方法。该方法将乡村运营区段划分为两个交替响应的小区段,... 为有效应对农村地区公交服务覆盖率低导致居民出行不便的问题,增强乘客作为出行主体的选择权,本文提出一种利用现有城乡公交固定线路资源,考虑乘客自主选择的区段交替响应式调度方法。该方法将乡村运营区段划分为两个交替响应的小区段,通过向量积法确定需求响应站点;设计两阶段调度模型,采用即时处理订单的方式实现实时响应,将已被服务的需求响应站点设为“临时固定站点”加速模型求解;并构建涵盖从出行需求产生到选择出发时间、上车站点、服务车次,以及拒单后二次选择等全过程的自主选择机制。以江苏省宿迁市泗阳县4路城乡公交为例,设计不同客流强度下的数值实验。实验结果表明:区段交替响应在大客流场景下,每个需求响应站点的平均下车人数为3.76人;不同客流强度下,自主选择机制使15.79%~34.50%的乘客做出个性化选择,至高提升13.51%的票价收入;通过收取服务票价的方式均衡额外成本,平均每增加1%的行驶距离可提升1.53%的票价收入。结果表明:区段交替响应可有效集中出行时间相似且到达站点在同一个区段内的乘客,减少运营成本;考虑乘客自主选择可完整刻画乘客出行全过程行为,并提高系统收入。 展开更多
关键词 智能交通 区段交替响应 两阶段调度 城乡公交 乘客选择 向量积法
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基于结构对应学习的大跨桥梁涡激振动识别方法研究
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作者 万春风 赵文龙 +2 位作者 周珍伟 曹素功 胡皓 《桥梁建设》 北大核心 2025年第3期73-80,共8页
为解决部分大跨桥梁缺少历史涡激振动数据而无法进行涡激振动识别的问题,提出一种基于结构对应学习(Structural Correspondence Learning,SCL)的大跨桥梁涡激振动识别方法。该方法首先将含有涡激振动数据与标签的参考桥梁数据集设为源域... 为解决部分大跨桥梁缺少历史涡激振动数据而无法进行涡激振动识别的问题,提出一种基于结构对应学习(Structural Correspondence Learning,SCL)的大跨桥梁涡激振动识别方法。该方法首先将含有涡激振动数据与标签的参考桥梁数据集设为源域,需要识别的目标桥梁数据作为目标域,在时间序列上截取加速度数据提取涡激振动特征;然后使用SCL方法对领域间特征样本进行对齐,将源域变为自适应源域;最后使用自适应源域样本训练实现目标桥梁的实时涡激振动智能识别。选取内陆地区某一较少发生涡激振动的钢-混组合大跨悬索桥作为目标桥梁,沿海地区某一较为频繁发生涡激振动的大跨悬索桥作为参考桥梁,采用所提方法对目标桥梁进行涡激振动智能识别与早期预警,以验证所提方法的有效性。结果表明:所提方法可利用其它已发生涡激振动桥梁的振动信号,通过迁移学习来识别目标桥梁的涡激振动;相较于不使用迁移学习的基准模型,所提方法取得了更优的结果,且更早地探测到了涡激振动的发生,具有较好的适用性与准确性,可为大跨桥梁涡激振动早期预警与控制提供技术支撑。 展开更多
关键词 大跨桥梁 涡激振动 结构健康监测 结构对应学习 迁移学习 特征向量 识别方法
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基于地震资料计算断层滑动矢量的四维搜索区域古应力计算方法
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作者 杨宇 吉礼东 +3 位作者 刘芳 颜平 徐春阳 吴昌荣 《石油地球物理勘探》 北大核心 2025年第2期431-439,共9页
区域古应力控制断层与裂缝的发育,因此准确了解区域古应力对油气勘探与开发具有重要意义。基于Wallace-Bott假设,采用断层滑动矢量结合四维搜索法可以较为准确地计算区域古应力。目前,一般使用地面露头的断层擦痕表示断层的滑动矢量,但... 区域古应力控制断层与裂缝的发育,因此准确了解区域古应力对油气勘探与开发具有重要意义。基于Wallace-Bott假设,采用断层滑动矢量结合四维搜索法可以较为准确地计算区域古应力。目前,一般使用地面露头的断层擦痕表示断层的滑动矢量,但是地面擦痕反映的地面应力状态与地下应力状态存在差异。因此,引入一种基于三维地震资料直接计算断层滑动矢量的方法。首先,构建垂直或水平地震剖面并读取视走向滑移与视倾斜滑移,并通过数学方法计算断层滑动矢量;然后,在此基础上采用四维搜索法计算区域古应力。以新场须二气藏M区块为例,计算了两条断层的18个断层滑动矢量,并将其随机分为5组,采用四维搜索法分别计算每一组的区域古应力,并评价区域古应力张量在断面上的剪切应力方向与实际断层滑移方向的偏差(RUP值)。结果显示,5组方案平均RUP值均小于50%,表明该方法计算区域古应力的结果准确,可以推广应用于类似地区。 展开更多
关键词 地震资料 断层滑动矢量 古应力反演 四维搜索法
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基于离散矢量模型的微电机转子二次去重动平衡研究
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作者 姜东 薛子旻 +3 位作者 鲁镇荣 李梦瑄 陈伟宇 杭晓晨 《振动工程学报》 北大核心 2025年第7期1486-1495,共10页
针对微电机转子初始不平衡量较大时动平衡效果不佳的问题,提出了一种基于离散矢量模型的切削去重不平衡校正方法。根据铣刀和转子的参数建立离散矢量去重模型,基于边缘曲线离散点的积分计算得到了二次去重情况下切削等效质量与切削深度... 针对微电机转子初始不平衡量较大时动平衡效果不佳的问题,提出了一种基于离散矢量模型的切削去重不平衡校正方法。根据铣刀和转子的参数建立离散矢量去重模型,基于边缘曲线离散点的积分计算得到了二次去重情况下切削等效质量与切削深度的对应关系,通过与三维模型仿真数据比较验证了模型的精度。采用5个微电机转子进行试验验证,在转子初始不平衡量高于100 mg的情况下,能实现90%以上的总去重率,并将剩余不平衡等效质量控制在10 mg以下,被测转子均符合G1.0精度等级。表明该方法能够在初始不平衡量较大时提高微电机转子动平衡精度。 展开更多
关键词 转子动平衡 微电机 切削去重不平衡校正方法 离散矢量模型 初始不平衡量
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航空高光谱图像的湖泊富营养化评价方法
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作者 黄岩 方彦奇 +5 位作者 徐明钻 石剑龙 杨奎 祁超 梁森 季岩 《红外与激光工程》 北大核心 2025年第5期84-91,共8页
针对目前湖泊水体富营养化评价中水质参数定量反演困难、数据多以地面或低空间分辨率数据为主的现状,采用0.5 m空间分辨率的航空高光谱遥感数据,结合地面实测数据开展水质参数定量反演研究,并基于反演结果进行湖泊水体富营养化评价。首... 针对目前湖泊水体富营养化评价中水质参数定量反演困难、数据多以地面或低空间分辨率数据为主的现状,采用0.5 m空间分辨率的航空高光谱遥感数据,结合地面实测数据开展水质参数定量反演研究,并基于反演结果进行湖泊水体富营养化评价。首先,对预处理后的水体表面离水反射率进行4种数学变换并与水质参数进行相关性分析,选择相关性较高的一阶微分,使用竞争性自适应重加权算法进行特征提取。然后采用基于量子粒子群(QPSO)参数优化的最小二乘支持向量回归(LSSVR)算法进行水质参数反演模型的构建,使用决定系数(R^(2))和均方根误差评价模型精度,并进行对比分析。最后进行综合营养状态指数计算,与实测值进行比较分析,并基于航空高光谱数据开展研究区湖泊水体富营养化评价。结果表明:1)基于QPSO-LSSVR方法的水质参数反演模型精度最高(R^(2)>0.8);2)综合营养指数结果准确,反演值与实测值的平均相对误差为0.91%,均方根误差为0.50;3)研究区水体富营养化评价结果空间分辨率高,能从面上精确、细致地反应湖水营养状态分布情况。该方法实现了水体富营养化的高精度快速评价。 展开更多
关键词 航空高光谱图像 湖泊富营养化 最小二乘支持向量回归 综合营养状态指数法
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同源重组法衣原体RNA聚合酶表达载体的构建和应用
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作者 石禹 包小峰 《医学研究与战创伤救治》 北大核心 2025年第2期113-119,共7页
目的利用同源重组法构建衣原体RNA聚合酶(cRNAP)各亚基质粒,转化入大肠埃希菌中表达蛋白并探讨其在衣原体转录调控中的应用。方法根据Gen Bank中的基因序列设计特异性引物,聚合酶链式反应(PCR)扩增cRNAP各亚基的目的基因片段,对PCR产物... 目的利用同源重组法构建衣原体RNA聚合酶(cRNAP)各亚基质粒,转化入大肠埃希菌中表达蛋白并探讨其在衣原体转录调控中的应用。方法根据Gen Bank中的基因序列设计特异性引物,聚合酶链式反应(PCR)扩增cRNAP各亚基的目的基因片段,对PCR产物进行纯化,质粒载体进行质粒抽提、质粒酶切、胶回收等处理。采用同源重组法将基因片段插入到目的载体进行片段融合构建cRNAP各亚基表达质粒,融合产物转化后用特异性引物进行PCR验证和测序。构建好的融合产物转化入大肠埃希菌化学感受态细胞表达蛋白。结果成功构建cRNAP各亚基质粒并成功表达cRNAP各亚基蛋白,研究了衣原体转录调节因子GrgA与cRNAP的直接相互作用是GrgA与cRNAP的α、β′亚基有结合,与β亚基无结合。两者可以作为衣原体感染治疗和预防的药物作用新靶点。结论同源重组法可用于构建cRNAP各亚基质粒并成功表达相应蛋白,并用于研究转录调节因子GrgA与cRNAP各亚基蛋白的结合位点,为衣原体感染治疗和预防药物作用新靶点的研究提供参考信息。 展开更多
关键词 同源重组法 衣原体 cRNAP 亚基 质粒载体 融合蛋白
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