Avian influenza virus is one of the main pathogens causing avian influenza.In this experiment,the avian influenza hemagglutinin(HA)gene was transferred into tobacco by Agrobacterium tumefaciens-mediated method using k...Avian influenza virus is one of the main pathogens causing avian influenza.In this experiment,the avian influenza hemagglutinin(HA)gene was transferred into tobacco by Agrobacterium tumefaciens-mediated method using kanamycin as a resistance marker to produce HA type edible vaccine designed for avian influenza hemagglutinin protein.The fusion of cholera toxin B subgene(CTB)and avian influenza HA was initiated by CaMV35S promoter,and then transformed into Nicotiana benthamiana to induce callus and adventitious bud differentiation,bud elongation,and growth and root induction.The total genomic DNA of 28 transgenic tobacco plants was extracted,and the CTB sequence of the CTB-HA fusion gene was used as a template to design primers for PCR amplification.Eight of them were positive,and four of them were expressed at the RNA level after RNA extraction and RT-PCR amplification.In western blot detection of protein extraction,two strains were shown at the position of the target protein.The results provided material support for the preparation of a transgenic plant oral vaccine against HA infection,and provided a theoretical basis for accelerating the development of a transgenic plant vaccine.展开更多
A wide variety of human tumors express interleukin10 (IL-10) for reasons poorly understood. We haveanalysed the effect of spontaneous IL-10 expression by amouse tumor (J558L) on its immunparalysing effect.Because cros...A wide variety of human tumors express interleukin10 (IL-10) for reasons poorly understood. We haveanalysed the effect of spontaneous IL-10 expression by amouse tumor (J558L) on its immunparalysing effect.Because cross-priming" of T cells by host antigenpresenting cells for MHC class I restricted tumor antigensis a major pathway for induction of tumor immunity andthat is enhanced by granulocyte-macrophage colony-stimulating factor (GM-CSF), we expressed this cytokinein J558L cells. GM-CSF secreting cells were not展开更多
Aiming at the problem on cooperative air-defense of surface warship formation, this paper maps the cooperative airdefense system of systems （SoS） for surface warship formation （CASoSSWF） to the biological immune s...Aiming at the problem on cooperative air-defense of surface warship formation, this paper maps the cooperative airdefense system of systems （SoS） for surface warship formation （CASoSSWF） to the biological immune system （BIS） according to the similarity of the defense mechanism and characteristics between the CASoSSWF and the BIS, and then designs the models of components and the architecture for a monitoring agent, a regulating agent, a killer agent, a pre-warning agent and a communicating agent by making use of the theories and methods of the artificial immune system, the multi-agent system （MAS）, the vaccine and the danger theory （DT）. Moreover a new immune multi-agent model using vaccine based on DT （IMMUVBDT） for the cooperative air-defense SoS is advanced. The immune response and immune mechanism of the CASoSSWF are analyzed. The model has a capability of memory, evolution, commendable dynamic environment adaptability and self-learning, and embodies adequately the cooperative air-defense mechanism for the CASoSSWF. Therefore it shows a novel idea for the CASoSSWF which can provide conception models for a surface warship formation operation simulation system.展开更多
Finding out reasonable structures from bulky data is one of the difficulties in modeling of Bayesian network （BN）, which is also necessary in promoting the application of BN. This pa- per proposes an immune algorith...Finding out reasonable structures from bulky data is one of the difficulties in modeling of Bayesian network （BN）, which is also necessary in promoting the application of BN. This pa- per proposes an immune algorithm based method （BN-IA） for the learning of the BN structure with the idea of vaccination. Further- more, the methods on how to extract the effective vaccines from local optimal structure and root nodes are also described in details. Finally, the simulation studies are implemented with the helicopter convertor BN model and the car start BN model. The comparison results show that the proposed vaccines and the BN-IA can learn the BN structure effectively and efficiently.展开更多
Cytokines provided locally at tbe tumor site mayinitiate an effective anti-tumor immune responsewhich leads to rejection of a tumor which otherwisegrows progressively. Experimentillly, this can betested by gene transf...Cytokines provided locally at tbe tumor site mayinitiate an effective anti-tumor immune responsewhich leads to rejection of a tumor which otherwisegrows progressively. Experimentillly, this can betested by gene transfer into cultured tumor cellsfollowed by the analysis of the tumorigenicity of suchgenetically engincered cells. This approach allows展开更多
Gene modification of tumor cells to express a varietyof cytokines such as IL-2 or the co-stimulatory moleculeB7. 1 led to increased immunogenicity and reducedtumorigenicity of tumors has several models and thisprocess...Gene modification of tumor cells to express a varietyof cytokines such as IL-2 or the co-stimulatory moleculeB7. 1 led to increased immunogenicity and reducedtumorigenicity of tumors has several models and thisprocess involves T cells. We have previously reporteddecreased tumorigenicity of the murine plasmacytomaJ558L (MHC class Ⅰ^+ and class Ⅱ^-) expressing IL-2 orB7. l. When systemic immunity was analyzed,展开更多
Oncolysate, a debris of tumor cells, has been provento be effective in tumor active immunotherapy, it wasreported that the vaccinia virus, especially recombinantvaccinia viruses encoding human IL-2 (rVV-IL-2 ),enhance...Oncolysate, a debris of tumor cells, has been provento be effective in tumor active immunotherapy, it wasreported that the vaccinia virus, especially recombinantvaccinia viruses encoding human IL-2 (rVV-IL-2 ),enhanced the immunogenicity of transfected tumor cells.In this experiment, the murine melanoma cell B16-F10oncolysates trans fected by rVV-IL-2 (IL-2VBO) wereused as vaccine. The IL-2VBO or TK-VBO was preparedby incubating B16-F10 cells with rVV-IL-2 or rVV-TK展开更多
The adaptive immune system produces a large and diverse set of antibodies,each with an individual evolutionary and clonal history.This so called"antibody repertoire"protects each individual against insults s...The adaptive immune system produces a large and diverse set of antibodies,each with an individual evolutionary and clonal history.This so called"antibody repertoire"protects each individual against insults such as infection and cancer,and responds to vaccination with B cell proliferation in response to the antigenic stimulation.Hybridomas and antigen-specific FACSbased analysis have given us much insight on how the immune system generates the complex and diverse immune response required to protect the body from the wide variety of potential pathogens.However,these methods have not been sufficient to make global and unbiased characterizations of the clonal structure of the immune system of a particular individual。展开更多
Dendritic cells (DC) play a critical role in theinduction of immune responses, and are utilized as potentadjuvants in cancer immunotherapy. Several strategies forloading DC with tumor antigens have been developed.Vacc...Dendritic cells (DC) play a critical role in theinduction of immune responses, and are utilized as potentadjuvants in cancer immunotherapy. Several strategies forloading DC with tumor antigens have been developed.Vaccination with tumor antigen-loaded DC wasdemonstrated to be capable of inducing antitumorimmunity, but some limitations still exist. So展开更多
Tumor cells escape host immune surveillance bydown-regulation of MHC and/or co-stimulatorymolecules.Anti-tumor immune responses are mediated primarily by T cells.A deficiency in either MHC or co-stimulatory molecules ...Tumor cells escape host immune surveillance bydown-regulation of MHC and/or co-stimulatorymolecules.Anti-tumor immune responses are mediated primarily by T cells.A deficiency in either MHC or co-stimulatory molecules on tumor cells is associated with a failure to induce anti-tumor immunity.展开更多
We have previously shown that tumor cellsgenetically modified to co-express IL-4 or IL-7 and B7.1have increased immunogenicity and provided vaccinessuperior to single gene transfectants or tumor cells/adjuvant mixture...We have previously shown that tumor cellsgenetically modified to co-express IL-4 or IL-7 and B7.1have increased immunogenicity and provided vaccinessuperior to single gene transfectants or tumor cells/adjuvant mixture.Tumor antigens can be presented eitherdirectly by tumor cells or be indirectly represented byhost APC(cross-priming).Here we asked whether B7.1and IL-7 or IL-4 improved in an additive fashion one pathway of antigen presentation or complemented each other by improving preferentially different pathways of antigen presentation.展开更多
基金Supported by the Natural Science Foundation of Heilongjiang Province(LH2021C032)。
文摘Avian influenza virus is one of the main pathogens causing avian influenza.In this experiment,the avian influenza hemagglutinin(HA)gene was transferred into tobacco by Agrobacterium tumefaciens-mediated method using kanamycin as a resistance marker to produce HA type edible vaccine designed for avian influenza hemagglutinin protein.The fusion of cholera toxin B subgene(CTB)and avian influenza HA was initiated by CaMV35S promoter,and then transformed into Nicotiana benthamiana to induce callus and adventitious bud differentiation,bud elongation,and growth and root induction.The total genomic DNA of 28 transgenic tobacco plants was extracted,and the CTB sequence of the CTB-HA fusion gene was used as a template to design primers for PCR amplification.Eight of them were positive,and four of them were expressed at the RNA level after RNA extraction and RT-PCR amplification.In western blot detection of protein extraction,two strains were shown at the position of the target protein.The results provided material support for the preparation of a transgenic plant oral vaccine against HA infection,and provided a theoretical basis for accelerating the development of a transgenic plant vaccine.
文摘A wide variety of human tumors express interleukin10 (IL-10) for reasons poorly understood. We haveanalysed the effect of spontaneous IL-10 expression by amouse tumor (J558L) on its immunparalysing effect.Because cross-priming" of T cells by host antigenpresenting cells for MHC class I restricted tumor antigensis a major pathway for induction of tumor immunity andthat is enhanced by granulocyte-macrophage colony-stimulating factor (GM-CSF), we expressed this cytokinein J558L cells. GM-CSF secreting cells were not
文摘Aiming at the problem on cooperative air-defense of surface warship formation, this paper maps the cooperative airdefense system of systems （SoS） for surface warship formation （CASoSSWF） to the biological immune system （BIS） according to the similarity of the defense mechanism and characteristics between the CASoSSWF and the BIS, and then designs the models of components and the architecture for a monitoring agent, a regulating agent, a killer agent, a pre-warning agent and a communicating agent by making use of the theories and methods of the artificial immune system, the multi-agent system （MAS）, the vaccine and the danger theory （DT）. Moreover a new immune multi-agent model using vaccine based on DT （IMMUVBDT） for the cooperative air-defense SoS is advanced. The immune response and immune mechanism of the CASoSSWF are analyzed. The model has a capability of memory, evolution, commendable dynamic environment adaptability and self-learning, and embodies adequately the cooperative air-defense mechanism for the CASoSSWF. Therefore it shows a novel idea for the CASoSSWF which can provide conception models for a surface warship formation operation simulation system.
基金supported by the National Natural Science Foundation of China(7110111671271170)+1 种基金the Program for New Century Excellent Talents in University(NCET-13-0475)the Basic Research Foundation of NPU(JC20120228)
文摘Finding out reasonable structures from bulky data is one of the difficulties in modeling of Bayesian network （BN）, which is also necessary in promoting the application of BN. This pa- per proposes an immune algorithm based method （BN-IA） for the learning of the BN structure with the idea of vaccination. Further- more, the methods on how to extract the effective vaccines from local optimal structure and root nodes are also described in details. Finally, the simulation studies are implemented with the helicopter convertor BN model and the car start BN model. The comparison results show that the proposed vaccines and the BN-IA can learn the BN structure effectively and efficiently.
文摘Cytokines provided locally at tbe tumor site mayinitiate an effective anti-tumor immune responsewhich leads to rejection of a tumor which otherwisegrows progressively. Experimentillly, this can betested by gene transfer into cultured tumor cellsfollowed by the analysis of the tumorigenicity of suchgenetically engincered cells. This approach allows
文摘Gene modification of tumor cells to express a varietyof cytokines such as IL-2 or the co-stimulatory moleculeB7. 1 led to increased immunogenicity and reducedtumorigenicity of tumors has several models and thisprocess involves T cells. We have previously reporteddecreased tumorigenicity of the murine plasmacytomaJ558L (MHC class Ⅰ^+ and class Ⅱ^-) expressing IL-2 orB7. l. When systemic immunity was analyzed,
文摘Oncolysate, a debris of tumor cells, has been provento be effective in tumor active immunotherapy, it wasreported that the vaccinia virus, especially recombinantvaccinia viruses encoding human IL-2 (rVV-IL-2 ),enhanced the immunogenicity of transfected tumor cells.In this experiment, the murine melanoma cell B16-F10oncolysates trans fected by rVV-IL-2 (IL-2VBO) wereused as vaccine. The IL-2VBO or TK-VBO was preparedby incubating B16-F10 cells with rVV-IL-2 or rVV-TK
基金supported by the National Institutes of Health grant U19 A1057229(M.M. D.,X.H.,H.B.G.and S.R.Q.)a National Institutes of Health Pathway to Independence Award K99 AG040149(N.J.)a National Science Foundation graduate fellowship(J.A.W.)
文摘The adaptive immune system produces a large and diverse set of antibodies,each with an individual evolutionary and clonal history.This so called"antibody repertoire"protects each individual against insults such as infection and cancer,and responds to vaccination with B cell proliferation in response to the antigenic stimulation.Hybridomas and antigen-specific FACSbased analysis have given us much insight on how the immune system generates the complex and diverse immune response required to protect the body from the wide variety of potential pathogens.However,these methods have not been sufficient to make global and unbiased characterizations of the clonal structure of the immune system of a particular individual。
文摘Dendritic cells (DC) play a critical role in theinduction of immune responses, and are utilized as potentadjuvants in cancer immunotherapy. Several strategies forloading DC with tumor antigens have been developed.Vaccination with tumor antigen-loaded DC wasdemonstrated to be capable of inducing antitumorimmunity, but some limitations still exist. So
文摘Tumor cells escape host immune surveillance bydown-regulation of MHC and/or co-stimulatorymolecules.Anti-tumor immune responses are mediated primarily by T cells.A deficiency in either MHC or co-stimulatory molecules on tumor cells is associated with a failure to induce anti-tumor immunity.
文摘We have previously shown that tumor cellsgenetically modified to co-express IL-4 or IL-7 and B7.1have increased immunogenicity and provided vaccinessuperior to single gene transfectants or tumor cells/adjuvant mixture.Tumor antigens can be presented eitherdirectly by tumor cells or be indirectly represented byhost APC(cross-priming).Here we asked whether B7.1and IL-7 or IL-4 improved in an additive fashion one pathway of antigen presentation or complemented each other by improving preferentially different pathways of antigen presentation.
