Eclogite was firstly discovered at the Da Qaidam region (Yang,et al., 1998), and then in the Xitieshan and Dulan regions in 1999, constituting an over 350km long high\|pressure metamorphic belt in the northeastern Qin...Eclogite was firstly discovered at the Da Qaidam region (Yang,et al., 1998), and then in the Xitieshan and Dulan regions in 1999, constituting an over 350km long high\|pressure metamorphic belt in the northeastern Qinghai—Tibet plateau. Eclogites occur as pods in the garnet\|muscovite gneiss of the Dakendaban Group (or called Shaliuhe Group in Dulan) of Upper Proterozoic age. In general, the pods of eclogite vary in size; most of them are less than 20m×10m, some large ones up to about 100m×50m. The eclogite\|hosted gneiss is pale\|gray in color, consisting mainly plagioclase and quartz, and minor muscovite (5%～10% in vol.) and garnet (1%～2%). Some of the country rocks of eclogite are mica\|quartz\|(feldspar) schist, quartzite, and ultramafic rocks, the latter also occur in blocks.Over 50 pods were found in a belt of 10km×3km in the Da Qaidam region (No.1 location). Only a few pods of eclogite were found in the Xitieshan region in 1999 field expedition (No.2 location). Eclogite in Dulan occurs in the Proterozoic strata of Shaliuhe Group (same as the Dakendaban Group but with a different name). The eclogites in the Dulan region (No.3 location) expose about 10km wide in SN and an unknown length in EW, and can be subdivided into two belts, the North Eclogite Belt of Dulan (NEBD) and the South Eclogite Belt of Dulan (SEBD).展开更多
The Sulu ultra-high pressure(UHP)metamorphic belt in Eastern China is well known as the eastern extension of the Qingling-Dabie orogenic belt formed by subduction and collision between the Sino-Korean and Yangtze crat...The Sulu ultra-high pressure(UHP)metamorphic belt in Eastern China is well known as the eastern extension of the Qingling-Dabie orogenic belt formed by subduction and collision between the Sino-Korean and Yangtze cratons.The main hole of the Chinese Continental Scientific Drilling(CCSD)project is located at the southern segment of the Sulu UHP metamorphic belt(34°25′N/118°40′E),about 17 km southwest of Donghai County.Integrated geophysical investigations using gravity,magnetic,deep展开更多
本研究以汉麻分离蛋白(Hemp Protein Isolate,HPI)为原料,通过超高压辅助酶解反应对HPI进行改性,测定不同压力下汉麻蛋白酶解产物(hydrolysate of hemp protein isolate,HPIH)的聚丙烯酰胺凝胶电泳(SDS polyacrylamide gelelectrophores...本研究以汉麻分离蛋白(Hemp Protein Isolate,HPI)为原料,通过超高压辅助酶解反应对HPI进行改性,测定不同压力下汉麻蛋白酶解产物(hydrolysate of hemp protein isolate,HPIH)的聚丙烯酰胺凝胶电泳(SDS polyacrylamide gelelectrophoresis,SDS-PAGE)电泳特性、表面疏水性、巯基含量、傅立叶红外光谱和内源荧光光谱分析改性前后汉麻分离蛋白的结构变化。结果表明,超高压(ultra-high pressure,UHP)(0.1、100、200、300 MPa)处理对HPI酶解反应具有一定的辅助作用,且随压力的升高酶解反应程度逐渐增大,分子量逐渐降低;HPI经改性后,疏水性基团逐渐暴露,表面疏水性随压力的增大先上升后下降,且变化差异性显著(P<0.05),在200 MPa时表面疏水性达到最大;酶解反应后,HPIH游离巯基含量显著降低(P<0.05),而表面巯基含量随压力增大呈先上升后下降的趋势;通过测定改性前后蛋白质氨基酸组成及含量可知,改性前后HPI氨基酸组成不变,但各氨基酸含量存在不同程度下降;由傅立叶红外光谱图可以看出,与HPI相比,HPIH的吸收峰强度、峰型及峰面积等均发生不同程度变化,说明超高压辅助酶解反应使蛋白质二级结构发生改变;内源荧光光谱显示,HPIH荧光强度增大且最大发射波长发生红移,说明酶解反应改变了HPI的三级结构;抗氧化活性结果表明,适当的压力处理可有效提升酶解产物的抗氧化能力,当压力为200 MPa时,HPIH的DPPH、ABTS^(+)自由基清除能力及还原能力达到最高。综上所述,超高压辅助酶解改性处理能显著改变汉麻分离蛋白的二、三级结构,暴露出疏水基团等活性基团,从而提高其抗氧化性。展开更多
文摘Eclogite was firstly discovered at the Da Qaidam region (Yang,et al., 1998), and then in the Xitieshan and Dulan regions in 1999, constituting an over 350km long high\|pressure metamorphic belt in the northeastern Qinghai—Tibet plateau. Eclogites occur as pods in the garnet\|muscovite gneiss of the Dakendaban Group (or called Shaliuhe Group in Dulan) of Upper Proterozoic age. In general, the pods of eclogite vary in size; most of them are less than 20m×10m, some large ones up to about 100m×50m. The eclogite\|hosted gneiss is pale\|gray in color, consisting mainly plagioclase and quartz, and minor muscovite (5%～10% in vol.) and garnet (1%～2%). Some of the country rocks of eclogite are mica\|quartz\|(feldspar) schist, quartzite, and ultramafic rocks, the latter also occur in blocks.Over 50 pods were found in a belt of 10km×3km in the Da Qaidam region (No.1 location). Only a few pods of eclogite were found in the Xitieshan region in 1999 field expedition (No.2 location). Eclogite in Dulan occurs in the Proterozoic strata of Shaliuhe Group (same as the Dakendaban Group but with a different name). The eclogites in the Dulan region (No.3 location) expose about 10km wide in SN and an unknown length in EW, and can be subdivided into two belts, the North Eclogite Belt of Dulan (NEBD) and the South Eclogite Belt of Dulan (SEBD).
文摘The Sulu ultra-high pressure(UHP)metamorphic belt in Eastern China is well known as the eastern extension of the Qingling-Dabie orogenic belt formed by subduction and collision between the Sino-Korean and Yangtze cratons.The main hole of the Chinese Continental Scientific Drilling(CCSD)project is located at the southern segment of the Sulu UHP metamorphic belt(34°25′N/118°40′E),about 17 km southwest of Donghai County.Integrated geophysical investigations using gravity,magnetic,deep
文摘本研究以汉麻分离蛋白(Hemp Protein Isolate,HPI)为原料,通过超高压辅助酶解反应对HPI进行改性,测定不同压力下汉麻蛋白酶解产物(hydrolysate of hemp protein isolate,HPIH)的聚丙烯酰胺凝胶电泳(SDS polyacrylamide gelelectrophoresis,SDS-PAGE)电泳特性、表面疏水性、巯基含量、傅立叶红外光谱和内源荧光光谱分析改性前后汉麻分离蛋白的结构变化。结果表明,超高压(ultra-high pressure,UHP)(0.1、100、200、300 MPa)处理对HPI酶解反应具有一定的辅助作用,且随压力的升高酶解反应程度逐渐增大,分子量逐渐降低;HPI经改性后,疏水性基团逐渐暴露,表面疏水性随压力的增大先上升后下降,且变化差异性显著(P<0.05),在200 MPa时表面疏水性达到最大;酶解反应后,HPIH游离巯基含量显著降低(P<0.05),而表面巯基含量随压力增大呈先上升后下降的趋势;通过测定改性前后蛋白质氨基酸组成及含量可知,改性前后HPI氨基酸组成不变,但各氨基酸含量存在不同程度下降;由傅立叶红外光谱图可以看出,与HPI相比,HPIH的吸收峰强度、峰型及峰面积等均发生不同程度变化,说明超高压辅助酶解反应使蛋白质二级结构发生改变;内源荧光光谱显示,HPIH荧光强度增大且最大发射波长发生红移,说明酶解反应改变了HPI的三级结构;抗氧化活性结果表明,适当的压力处理可有效提升酶解产物的抗氧化能力,当压力为200 MPa时,HPIH的DPPH、ABTS^(+)自由基清除能力及还原能力达到最高。综上所述,超高压辅助酶解改性处理能显著改变汉麻分离蛋白的二、三级结构,暴露出疏水基团等活性基团,从而提高其抗氧化性。
