In this paper,we study the uniqueness of positive solutions to the following semilinear equations{-Δu=λ|x|^(α)ue^(u^(2)),in B_(1),u=0,onδB_(1)ueu2;in B_(1);u=0;on@B_(1);whereλ>0,α>-2;B_(1)denotes the unit ...In this paper,we study the uniqueness of positive solutions to the following semilinear equations{-Δu=λ|x|^(α)ue^(u^(2)),in B_(1),u=0,onδB_(1)ueu2;in B_(1);u=0;on@B_(1);whereλ>0,α>-2;B_(1)denotes the unit disk in R^(2):By delicate and relatively complicated computation of radial solutions to the above equation and the asymptotic expansion of solutions near the boundary of B_(1),the uniqueness of positive solutions is obtained.The results of this paper extend the uniqueness result for the semilinear equation with critical exponential growth in CHEN et al.(2022)to the case that includes a Henon term.展开更多
In this paper,by utilizing the Marcinkiewicz-Zygmund inequality and Rosenthal-type inequality of negatively superadditive dependent(NSD)random arrays and truncated method,we investigate the complete f-moment convergen...In this paper,by utilizing the Marcinkiewicz-Zygmund inequality and Rosenthal-type inequality of negatively superadditive dependent(NSD)random arrays and truncated method,we investigate the complete f-moment convergence of NSD random variables.We establish and improve a general result on the complete f-moment convergence for Sung’s type randomly weighted sums of NSD random variables under some general assumptions.As an application,we show the complete consistency for the randomly weighted estimator in a nonparametric regression model based on NSD errors.展开更多
An evolution inequality of Sobolev type involving a nonlinear convolution term is considered.By using the nonlinear capacity method and the contradiction argument,the non-existence of the nontrivial local weak solutio...An evolution inequality of Sobolev type involving a nonlinear convolution term is considered.By using the nonlinear capacity method and the contradiction argument,the non-existence of the nontrivial local weak solution is proved.展开更多
Accurate estimation of understory terrain has significant scientific importance for maintaining ecosystem balance and biodiversity conservation.Addressing the issue of inadequate representation of spatial heterogeneit...Accurate estimation of understory terrain has significant scientific importance for maintaining ecosystem balance and biodiversity conservation.Addressing the issue of inadequate representation of spatial heterogeneity when traditional forest topographic inversion methods consider the entire forest as the inversion unit,this study pro⁃poses a differentiated modeling approach to forest types based on refined land cover classification.Taking Puerto Ri⁃co and Maryland as study areas,a multi-dimensional feature system is constructed by integrating multi-source re⁃mote sensing data:ICESat-2 spaceborne LiDAR is used to obtain benchmark values for understory terrain,topo⁃graphic factors such as slope and aspect are extracted based on SRTM data,and vegetation cover characteristics are analyzed using Landsat-8 multispectral imagery.This study incorporates forest type as a classification modeling con⁃dition and applies the random forest algorithm to build differentiated topographic inversion models.Experimental re⁃sults indicate that,compared to traditional whole-area modeling methods(RMSE=5.06 m),forest type-based classi⁃fication modeling significantly improves the accuracy of understory terrain estimation(RMSE=2.94 m),validating the effectiveness of spatial heterogeneity modeling.Further sensitivity analysis reveals that canopy structure parame⁃ters(with RMSE variation reaching 4.11 m)exert a stronger regulatory effect on estimation accuracy compared to forest cover,providing important theoretical support for optimizing remote sensing models of forest topography.展开更多
Objective:Type 2 diabetes mellitus(T2DM)is characterized by insufficient insulin secretion and insulin resistance.Gypenosides(GPs)are the major bioactive saponins extracted from Gynostemma pentaphyllum.Previous studie...Objective:Type 2 diabetes mellitus(T2DM)is characterized by insufficient insulin secretion and insulin resistance.Gypenosides(GPs)are the major bioactive saponins extracted from Gynostemma pentaphyllum.Previous studies suggest that GPs have beneficial effects on T2DM,but the underlying mechanisms remain unclear.This study aims to investigate whether GPs exert therapeutic effects by influencing RNA N^(6)-methyladenosine(m6A)methylation modification,thereby regulating the downstream phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway.Methods:Expression levels and methylation changes of METTL3,METTL14,and FTO in T2DM were analyzed using public databases,and related pathways were explored via gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses.The main active components of GPs were screened from pharmacological databases,followed by compound-target network construction,enrichment analyses,and prediction of potential targets and pathways.A T2DM model was induced in Sprague-Dawley rats using a high-fat/high-sugar diet combined with low-dose streptozotocin.Rats were randomly divided into 4 groups:GPs-treated group(GPG,400 mg/kg),positive control group(PCG,metformin 100 mg/kg),normal saline control group(CONTROL),and T2DM model group(MODEL).Fasting blood glucose(FBG),oral glucose tolerance test(OGTT)-area under the glucose curve from 0 to 120 min(AUC0-120),fasting insulin(FINS),homeostasis model assessment of insulin resistance(HOMA-IR),serum inflammatory factors,and tissue pathology of pancreas and liver hematoxylin and eosin(HE)staining were assessed.Real-time fluorescence quantitative PCR and Western blotting were used to detect RNA and protein expression levels of METTL3,METTL14,FTO,PI3K,and AKT in pancreatic tissues.Molecular docking was applied to evaluate interactions between GPs’main components and METTL3,METTL14,and FTO to infer potential binding modes.Results:Bioinformatic analyses showed downregulation of METTL3/14 and upregulation of FTO in T2DM samples(all P<0.05),with enrichment in pathways related to insulin signaling,PI3K/AKT activation,oxidative stress response,and hormone secretion.Network pharmacology indicated that GPs components may act on targets involved in RNA modification and insulin-related pathways.In diabetic rats,GPs significantly reduced FBG,improved glucose tolerance,decreased HOMA-IR,and decreased the serum tumor necrosis factor-α(TNF-α)and interleukin(IL)-6 levels compared with MODEL(all P<0.05).Pancreatic pathology showed alleviated islet injury and improved cell morphology.GPs treatment upregulated METTL3/14 mRNA and protein levels,and down-regulated FTO mRNA/protein levels in pancreatic tissue(all P<0.05).PI3K and AKT expression levels increased(both P<0.05),consistent with activation of downstream signals related to glucose uptake and improved insulin sensitivity.Metformin also improved metabolic indices but exhibited a different regulatory pattern on m6A-related enzymes compared with GPs.Molecular docking revealed stable interactions between core GPs saponin structures and methyltransferase-like 3(METTL3),methyltransferase-like 14(METTL14),or obesityassociated protein(FTO),suggesting that GPs may directly or indirectly modulate m6A regulatory proteins.Conclusion:GPs can effectively improve glucose-metabolism disorders,reduce inflammation,and protect pancreatic tissue in T2DM rats.The mechanisms may be associated with METTL3/14 up-regulation and FTO down-regulation,leading to enhanced m6A methylation and subsequent activation of the PI3K/AKT signaling pathway.These findings provide strong evidence for GPs regulation of epigenetic m6A RNA modification and insulin-related downstream pathways,and suggest that natural compounds targeting m6A regulation may be explored in the future for metabolic disease interventions.展开更多
目的:对部分D表型孕妇进行免疫血清学和RHD基因型分析。方法:采用常规血型血清学方法鉴定孕妇RhD血型,并进行血型特异性抗体筛查和鉴定;采用序列特异性引物聚合酶链反应(polymerase chain reactionsequence specific primer,PCR-SSP)鉴...目的:对部分D表型孕妇进行免疫血清学和RHD基因型分析。方法:采用常规血型血清学方法鉴定孕妇RhD血型,并进行血型特异性抗体筛查和鉴定;采用序列特异性引物聚合酶链反应(polymerase chain reactionsequence specific primer,PCR-SSP)鉴定孕妇RHD基因型;采用多重连接依赖的探针扩增技术(multiplex ligationdependent probe amplification,MLPA)对孕妇及其配偶和女儿的RhD血型抗原进行基因分型及遗传分析。结果:该孕妇血清中检测出IgG抗-D,其抗体效价为1∶8。PCR-SSP结果显示,该孕妇RHD基因第3-6外显子缺失,经鉴定该孕妇RHD基因型为DVI type 3型。MLPA分析显示,该孕妇只有1条RHD等位基因,且缺失3-6外显子,其基因型为CDVIe/cde,其配偶为CDe/CDe纯合子基因型,女儿为CDe/CDVIe基因型。结论:准确的RhD血型鉴定对制定安全有效的临床输血策略和对育龄妇女采取恰当措施及预防新生儿溶血病具有重要意义。展开更多
目的:基于上皮细胞-间充质细胞转分化(EMT)学说观察化纤Ⅳ号方、艾灸以及二者相配合治疗肺纤维化大鼠Collagen TypeⅢ(Ⅲ-C)和PDGF的变化,探讨其治疗效应及生物学机制。方法:将鼠龄约为6周的SD大鼠随机分为空白组、模型组、化纤Ⅳ号方...目的:基于上皮细胞-间充质细胞转分化(EMT)学说观察化纤Ⅳ号方、艾灸以及二者相配合治疗肺纤维化大鼠Collagen TypeⅢ(Ⅲ-C)和PDGF的变化,探讨其治疗效应及生物学机制。方法:将鼠龄约为6周的SD大鼠随机分为空白组、模型组、化纤Ⅳ号方组、艾灸组、化纤Ⅳ号方与艾灸配合治疗组(简称为"灸药组"),治疗30 d后处死观察其肺组织病理改变,并检测其Collagen TypeⅢ、PDGF的基因和蛋白表达情况。结果:实时荧光定量结果显示:与空白组相比,各组Ⅲ-C和PDGF m RNA表达增高(P<0.05)。与模型组相比,各组的Ⅲ-C和PDGF m RNA表达有明显降低(P<0.01)。而各组中,灸药组疗效最明显,Ⅲ-C和PDGF的表达最低。蛋白免疫印迹法检测结果显示:与模型组相比各组的Ⅲ-C蛋白表达有差异。结论:1艾灸、化纤Ⅳ号方均可减轻博莱霉素诱导肺纤维化大鼠的肺纤维化程度。2艾灸配合化纤Ⅳ号方可减轻博莱霉素诱导肺纤维化大鼠的肺纤维化程度,且其效果优于单用艾灸或单用化纤Ⅳ号方。3艾灸、化纤Ⅳ号方及其二者配合使用不同程度阻抑博莱霉素诱导肺纤维化大鼠肺纤维化进程的效应机制,可能与通过调控其EMT过程中的Ⅲ-C和PDGF表达环节紧密相关。展开更多
利用粉煤灰合成Linde type F(K)沸石吸附重金属Ni2+,探讨吸附剂用量、初始pH、反应温度以及反应时间对Ni2+吸附效果的影响,同时进行吸附等温线和吸附动力学的数据模拟。结果表明,沸石投加量、初始pH以及反应温度均对Ni2+的去除效果影响...利用粉煤灰合成Linde type F(K)沸石吸附重金属Ni2+,探讨吸附剂用量、初始pH、反应温度以及反应时间对Ni2+吸附效果的影响,同时进行吸附等温线和吸附动力学的数据模拟。结果表明,沸石投加量、初始pH以及反应温度均对Ni2+的去除效果影响明显。随着沸石投加量的不断增大,Ni2+去除率不断提高,饱和吸附量逐渐减小。初始pH 7是沸石吸附Ni2+的最适pH。反应温度越高,沸石吸附Ni2+达到平衡的时间越短。沸石对Ni2+的吸附过程符合Langmuir吸附等温式;准一级动力学方程能较好地描述沸石对Ni2+的吸附行为。展开更多
介绍了基于ISO/IEC14443 TYPE B协议的13.56MHz的RFID阅读器的设计。给出了设计的硬件电路和防冲撞算法的软件实现,最后给出了示波器测试的实验结果。本文所用的阅读器芯片为瑞士μEM公司生产的模拟前端集成电路芯片EM4094,微控制器使用...介绍了基于ISO/IEC14443 TYPE B协议的13.56MHz的RFID阅读器的设计。给出了设计的硬件电路和防冲撞算法的软件实现,最后给出了示波器测试的实验结果。本文所用的阅读器芯片为瑞士μEM公司生产的模拟前端集成电路芯片EM4094,微控制器使用了ARM7芯片LPC2104,防冲撞算法使用传统的基于ALOHA的算法。展开更多
为进一步明确Avenin-like type b基因的功能特性,以小麦品种西农188为材料,通过设计简并引物克隆小麦Avenin-like type b基因,对克隆得到的序列进行同源性比对,分析编码蛋白的理化性质、结构特点,结合系统发育树、亚细胞定位预测及结构...为进一步明确Avenin-like type b基因的功能特性,以小麦品种西农188为材料,通过设计简并引物克隆小麦Avenin-like type b基因,对克隆得到的序列进行同源性比对,分析编码蛋白的理化性质、结构特点,结合系统发育树、亚细胞定位预测及结构域分析探讨其可能具有的功能特性,同时对该基因进行体外原核表达及品质功能相关分析。结果表明,从西农188克隆得到4条序列,Genbank登录号为KY794919~KY794922,其中KY794922在信号肽区段碱基缺失导致移码突变。KY794919~KY794921序列与Aveninlike type b家族中大麦、柱穗山羊草、二穗短柄草及节节麦的同源性较高。蛋白序列分析表明,Avenin-like type b编码氨基酸序列具有较高的保守性,其Cys的数目及位置也较为保守,具有两个典型的AAI结构域,推测其可能为一类具有α-淀粉酶抑制剂活性的蛋白。亚细胞定位显示,该类蛋白可能在合成加工后通过运输小泡转运至细胞外行使功能。SDS-PAGE分析表明,KY794919~KY794921体外原核表达成功。通过纯化表达产物及掺粉试验表明,KY794919~KY794921编码蛋白对小麦面粉品质均具有显著正向效应,但仅有KY794919可显著提高面粉吸水量进而延长面筋扩展时间,说明其可能在品质功能研究资源中更具有优越性。展开更多
Two japonica rice varieties, Longjing 20 （more tillers and curved panicle type, MCP） and Longjing 21 （few tillers and half erect panicle type, FEP）, were used to study the effects of row-spacing on canopy structur...Two japonica rice varieties, Longjing 20 （more tillers and curved panicle type, MCP） and Longjing 21 （few tillers and half erect panicle type, FEP）, were used to study the effects of row-spacing on canopy structure, morphological characteristics and yield. The results showed that the percentage of productive tiller reduced first, and increased afterwards as row-spacing increasing. The relationship between row spacing and the percentage of productive tiller fitted a quadratic regression. The effects of row spacing on leaf area index （LAI） at later tillering stage and the highest stem number per square meter also followed a quadratic regression relationship with increasing first and then reducing. The effects of row-spacing on primary branch were larger than the secondary branch in Longjing 20. However, the trend in Longjing 21 was opposite. The relationship between row spacing and seed setting rate of the secondary branch or panicle was negatively correlated. An extreme significant negative correlation was obtained between seed setting rate of secondary branch in Longjing 20. There was no significant positive correlation between row-spacing and yield in Longjing 20 （R2=0.68）. However, the negative correlation between row-spacing and yield of Longjing 21 was extremely significant （R2=–0.96＊＊）. The canopy structure of MCP was more sensitive to row-spacing. The positive correlation between row spacing and the length of the flag leaf （R2=0.89＊＊）, the width of the flag leaf （R2=0.85＊）, the length of the last internode （R2=0.85＊）, the length of the last 2nd internode （R2=0.96＊＊） or the length of the panicle （R2=0.91＊＊） was significant or extremely significant in Longjing 20, but not in Longjing 21. The wider row-spacing promoted the accumulation of the dry matter of panicle, stem and leaf and the yield formation in MCP. The best row-spacing in Longjing 20 was 30 cm. For Longjing 21, the narrower row-spacing was better. The best row-spacing of it was 21 cm. These results suggested that improved the population environment of MCP or the utilization of the free space in the field of FEP could be reached either by wider row-spacing or narrow row-spacing.展开更多
基金Supported by the Natural Science Foundation of China(12571122,12061010)。
文摘In this paper,we study the uniqueness of positive solutions to the following semilinear equations{-Δu=λ|x|^(α)ue^(u^(2)),in B_(1),u=0,onδB_(1)ueu2;in B_(1);u=0;on@B_(1);whereλ>0,α>-2;B_(1)denotes the unit disk in R^(2):By delicate and relatively complicated computation of radial solutions to the above equation and the asymptotic expansion of solutions near the boundary of B_(1),the uniqueness of positive solutions is obtained.The results of this paper extend the uniqueness result for the semilinear equation with critical exponential growth in CHEN et al.(2022)to the case that includes a Henon term.
基金supported by the National Social Science Fundation(Grant No.21BTJ040)the Project of Outstanding Young People in University of Anhui Province(Grant Nos.2023AH020037,SLXY2024A001).
文摘In this paper,by utilizing the Marcinkiewicz-Zygmund inequality and Rosenthal-type inequality of negatively superadditive dependent(NSD)random arrays and truncated method,we investigate the complete f-moment convergence of NSD random variables.We establish and improve a general result on the complete f-moment convergence for Sung’s type randomly weighted sums of NSD random variables under some general assumptions.As an application,we show the complete consistency for the randomly weighted estimator in a nonparametric regression model based on NSD errors.
基金Supported by Scientific Research Fund of Hunan Provincial Education Departmen(t23A0361)。
文摘An evolution inequality of Sobolev type involving a nonlinear convolution term is considered.By using the nonlinear capacity method and the contradiction argument,the non-existence of the nontrivial local weak solution is proved.
基金Supported by the National Natural Science Foundation of China(42401488,42071351)the National Key Research and Development Program of China(2020YFA0608501,2017YFB0504204)+4 种基金the Liaoning Revitalization Talents Program(XLYC1802027)the Talent Recruited Program of the Chinese Academy of Science(Y938091)the Project Supported Discipline Innovation Team of the Liaoning Technical University(LNTU20TD-23)the Liaoning Province Doctoral Research Initiation Fund Program(2023-BS-202)the Basic Research Projects of Liaoning Department of Education(JYTQN2023202)。
文摘Accurate estimation of understory terrain has significant scientific importance for maintaining ecosystem balance and biodiversity conservation.Addressing the issue of inadequate representation of spatial heterogeneity when traditional forest topographic inversion methods consider the entire forest as the inversion unit,this study pro⁃poses a differentiated modeling approach to forest types based on refined land cover classification.Taking Puerto Ri⁃co and Maryland as study areas,a multi-dimensional feature system is constructed by integrating multi-source re⁃mote sensing data:ICESat-2 spaceborne LiDAR is used to obtain benchmark values for understory terrain,topo⁃graphic factors such as slope and aspect are extracted based on SRTM data,and vegetation cover characteristics are analyzed using Landsat-8 multispectral imagery.This study incorporates forest type as a classification modeling con⁃dition and applies the random forest algorithm to build differentiated topographic inversion models.Experimental re⁃sults indicate that,compared to traditional whole-area modeling methods(RMSE=5.06 m),forest type-based classi⁃fication modeling significantly improves the accuracy of understory terrain estimation(RMSE=2.94 m),validating the effectiveness of spatial heterogeneity modeling.Further sensitivity analysis reveals that canopy structure parame⁃ters(with RMSE variation reaching 4.11 m)exert a stronger regulatory effect on estimation accuracy compared to forest cover,providing important theoretical support for optimizing remote sensing models of forest topography.
基金supported by College Students’Innovative Entrepreneurial Training Plan Program of Guizhou Province,China(S202210660105,S202310660055)。
文摘Objective:Type 2 diabetes mellitus(T2DM)is characterized by insufficient insulin secretion and insulin resistance.Gypenosides(GPs)are the major bioactive saponins extracted from Gynostemma pentaphyllum.Previous studies suggest that GPs have beneficial effects on T2DM,but the underlying mechanisms remain unclear.This study aims to investigate whether GPs exert therapeutic effects by influencing RNA N^(6)-methyladenosine(m6A)methylation modification,thereby regulating the downstream phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway.Methods:Expression levels and methylation changes of METTL3,METTL14,and FTO in T2DM were analyzed using public databases,and related pathways were explored via gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses.The main active components of GPs were screened from pharmacological databases,followed by compound-target network construction,enrichment analyses,and prediction of potential targets and pathways.A T2DM model was induced in Sprague-Dawley rats using a high-fat/high-sugar diet combined with low-dose streptozotocin.Rats were randomly divided into 4 groups:GPs-treated group(GPG,400 mg/kg),positive control group(PCG,metformin 100 mg/kg),normal saline control group(CONTROL),and T2DM model group(MODEL).Fasting blood glucose(FBG),oral glucose tolerance test(OGTT)-area under the glucose curve from 0 to 120 min(AUC0-120),fasting insulin(FINS),homeostasis model assessment of insulin resistance(HOMA-IR),serum inflammatory factors,and tissue pathology of pancreas and liver hematoxylin and eosin(HE)staining were assessed.Real-time fluorescence quantitative PCR and Western blotting were used to detect RNA and protein expression levels of METTL3,METTL14,FTO,PI3K,and AKT in pancreatic tissues.Molecular docking was applied to evaluate interactions between GPs’main components and METTL3,METTL14,and FTO to infer potential binding modes.Results:Bioinformatic analyses showed downregulation of METTL3/14 and upregulation of FTO in T2DM samples(all P<0.05),with enrichment in pathways related to insulin signaling,PI3K/AKT activation,oxidative stress response,and hormone secretion.Network pharmacology indicated that GPs components may act on targets involved in RNA modification and insulin-related pathways.In diabetic rats,GPs significantly reduced FBG,improved glucose tolerance,decreased HOMA-IR,and decreased the serum tumor necrosis factor-α(TNF-α)and interleukin(IL)-6 levels compared with MODEL(all P<0.05).Pancreatic pathology showed alleviated islet injury and improved cell morphology.GPs treatment upregulated METTL3/14 mRNA and protein levels,and down-regulated FTO mRNA/protein levels in pancreatic tissue(all P<0.05).PI3K and AKT expression levels increased(both P<0.05),consistent with activation of downstream signals related to glucose uptake and improved insulin sensitivity.Metformin also improved metabolic indices but exhibited a different regulatory pattern on m6A-related enzymes compared with GPs.Molecular docking revealed stable interactions between core GPs saponin structures and methyltransferase-like 3(METTL3),methyltransferase-like 14(METTL14),or obesityassociated protein(FTO),suggesting that GPs may directly or indirectly modulate m6A regulatory proteins.Conclusion:GPs can effectively improve glucose-metabolism disorders,reduce inflammation,and protect pancreatic tissue in T2DM rats.The mechanisms may be associated with METTL3/14 up-regulation and FTO down-regulation,leading to enhanced m6A methylation and subsequent activation of the PI3K/AKT signaling pathway.These findings provide strong evidence for GPs regulation of epigenetic m6A RNA modification and insulin-related downstream pathways,and suggest that natural compounds targeting m6A regulation may be explored in the future for metabolic disease interventions.
文摘目的:对部分D表型孕妇进行免疫血清学和RHD基因型分析。方法:采用常规血型血清学方法鉴定孕妇RhD血型,并进行血型特异性抗体筛查和鉴定;采用序列特异性引物聚合酶链反应(polymerase chain reactionsequence specific primer,PCR-SSP)鉴定孕妇RHD基因型;采用多重连接依赖的探针扩增技术(multiplex ligationdependent probe amplification,MLPA)对孕妇及其配偶和女儿的RhD血型抗原进行基因分型及遗传分析。结果:该孕妇血清中检测出IgG抗-D,其抗体效价为1∶8。PCR-SSP结果显示,该孕妇RHD基因第3-6外显子缺失,经鉴定该孕妇RHD基因型为DVI type 3型。MLPA分析显示,该孕妇只有1条RHD等位基因,且缺失3-6外显子,其基因型为CDVIe/cde,其配偶为CDe/CDe纯合子基因型,女儿为CDe/CDVIe基因型。结论:准确的RhD血型鉴定对制定安全有效的临床输血策略和对育龄妇女采取恰当措施及预防新生儿溶血病具有重要意义。
文摘目的:基于上皮细胞-间充质细胞转分化(EMT)学说观察化纤Ⅳ号方、艾灸以及二者相配合治疗肺纤维化大鼠Collagen TypeⅢ(Ⅲ-C)和PDGF的变化,探讨其治疗效应及生物学机制。方法:将鼠龄约为6周的SD大鼠随机分为空白组、模型组、化纤Ⅳ号方组、艾灸组、化纤Ⅳ号方与艾灸配合治疗组(简称为"灸药组"),治疗30 d后处死观察其肺组织病理改变,并检测其Collagen TypeⅢ、PDGF的基因和蛋白表达情况。结果:实时荧光定量结果显示:与空白组相比,各组Ⅲ-C和PDGF m RNA表达增高(P<0.05)。与模型组相比,各组的Ⅲ-C和PDGF m RNA表达有明显降低(P<0.01)。而各组中,灸药组疗效最明显,Ⅲ-C和PDGF的表达最低。蛋白免疫印迹法检测结果显示:与模型组相比各组的Ⅲ-C蛋白表达有差异。结论:1艾灸、化纤Ⅳ号方均可减轻博莱霉素诱导肺纤维化大鼠的肺纤维化程度。2艾灸配合化纤Ⅳ号方可减轻博莱霉素诱导肺纤维化大鼠的肺纤维化程度,且其效果优于单用艾灸或单用化纤Ⅳ号方。3艾灸、化纤Ⅳ号方及其二者配合使用不同程度阻抑博莱霉素诱导肺纤维化大鼠肺纤维化进程的效应机制,可能与通过调控其EMT过程中的Ⅲ-C和PDGF表达环节紧密相关。
文摘利用粉煤灰合成Linde type F(K)沸石吸附重金属Ni2+,探讨吸附剂用量、初始pH、反应温度以及反应时间对Ni2+吸附效果的影响,同时进行吸附等温线和吸附动力学的数据模拟。结果表明,沸石投加量、初始pH以及反应温度均对Ni2+的去除效果影响明显。随着沸石投加量的不断增大,Ni2+去除率不断提高,饱和吸附量逐渐减小。初始pH 7是沸石吸附Ni2+的最适pH。反应温度越高,沸石吸附Ni2+达到平衡的时间越短。沸石对Ni2+的吸附过程符合Langmuir吸附等温式;准一级动力学方程能较好地描述沸石对Ni2+的吸附行为。
文摘介绍了基于ISO/IEC14443 TYPE B协议的13.56MHz的RFID阅读器的设计。给出了设计的硬件电路和防冲撞算法的软件实现,最后给出了示波器测试的实验结果。本文所用的阅读器芯片为瑞士μEM公司生产的模拟前端集成电路芯片EM4094,微控制器使用了ARM7芯片LPC2104,防冲撞算法使用传统的基于ALOHA的算法。
文摘为进一步明确Avenin-like type b基因的功能特性,以小麦品种西农188为材料,通过设计简并引物克隆小麦Avenin-like type b基因,对克隆得到的序列进行同源性比对,分析编码蛋白的理化性质、结构特点,结合系统发育树、亚细胞定位预测及结构域分析探讨其可能具有的功能特性,同时对该基因进行体外原核表达及品质功能相关分析。结果表明,从西农188克隆得到4条序列,Genbank登录号为KY794919~KY794922,其中KY794922在信号肽区段碱基缺失导致移码突变。KY794919~KY794921序列与Aveninlike type b家族中大麦、柱穗山羊草、二穗短柄草及节节麦的同源性较高。蛋白序列分析表明,Avenin-like type b编码氨基酸序列具有较高的保守性,其Cys的数目及位置也较为保守,具有两个典型的AAI结构域,推测其可能为一类具有α-淀粉酶抑制剂活性的蛋白。亚细胞定位显示,该类蛋白可能在合成加工后通过运输小泡转运至细胞外行使功能。SDS-PAGE分析表明,KY794919~KY794921体外原核表达成功。通过纯化表达产物及掺粉试验表明,KY794919~KY794921编码蛋白对小麦面粉品质均具有显著正向效应,但仅有KY794919可显著提高面粉吸水量进而延长面筋扩展时间,说明其可能在品质功能研究资源中更具有优越性。
基金Supported by the National Key Technology R&D Program (2007BAD65B01-4)Science and Technology Development Plan of Heilongjiang Province in China (GB06B104-1-5)Key Technology R&D Program of Heilongjiang Province in China (GA09B102-3)
文摘Two japonica rice varieties, Longjing 20 （more tillers and curved panicle type, MCP） and Longjing 21 （few tillers and half erect panicle type, FEP）, were used to study the effects of row-spacing on canopy structure, morphological characteristics and yield. The results showed that the percentage of productive tiller reduced first, and increased afterwards as row-spacing increasing. The relationship between row spacing and the percentage of productive tiller fitted a quadratic regression. The effects of row spacing on leaf area index （LAI） at later tillering stage and the highest stem number per square meter also followed a quadratic regression relationship with increasing first and then reducing. The effects of row-spacing on primary branch were larger than the secondary branch in Longjing 20. However, the trend in Longjing 21 was opposite. The relationship between row spacing and seed setting rate of the secondary branch or panicle was negatively correlated. An extreme significant negative correlation was obtained between seed setting rate of secondary branch in Longjing 20. There was no significant positive correlation between row-spacing and yield in Longjing 20 （R2=0.68）. However, the negative correlation between row-spacing and yield of Longjing 21 was extremely significant （R2=–0.96＊＊）. The canopy structure of MCP was more sensitive to row-spacing. The positive correlation between row spacing and the length of the flag leaf （R2=0.89＊＊）, the width of the flag leaf （R2=0.85＊）, the length of the last internode （R2=0.85＊）, the length of the last 2nd internode （R2=0.96＊＊） or the length of the panicle （R2=0.91＊＊） was significant or extremely significant in Longjing 20, but not in Longjing 21. The wider row-spacing promoted the accumulation of the dry matter of panicle, stem and leaf and the yield formation in MCP. The best row-spacing in Longjing 20 was 30 cm. For Longjing 21, the narrower row-spacing was better. The best row-spacing of it was 21 cm. These results suggested that improved the population environment of MCP or the utilization of the free space in the field of FEP could be reached either by wider row-spacing or narrow row-spacing.
