Dear Editor,The tuberculin skin test(TST)reagents have continuously improved,with the ESAT6-CFP10(EC)test having recently been introduced,but are seldom based on the direction of the delayed-type hypersensitivity(DTH)...Dear Editor,The tuberculin skin test(TST)reagents have continuously improved,with the ESAT6-CFP10(EC)test having recently been introduced,but are seldom based on the direction of the delayed-type hypersensitivity(DTH)mechanism.Previous studies only partially showed the infiltration and activation of immune cells and the production of cytokines of the skin induration[1,2],and lack the detailed measurements of cell proportions and gene expression in the DTH response.Therefore,in this study,we revealed the comprehensive characteristics of DTH by single-cell RNA sequencing(scRNA-seq)in the guinea pig tuberculosis(TB)model[Experimental Animal Welfare Ethics Committee,Beijing Tuberculosis and Thoracic Tumor Research Institute(2021-064)].展开更多
目的:研究结核菌素(Purified Protein Derivation,PPD)皮试在诊断潜伏结核感染和结核病中的意义。方法:2008年1月~2009年12月怀疑结核感染的2714例小儿PPD皮试,结果登记并随访分析,PPD阳性率在不同年龄组间、不同性别、有无卡介苗接种...目的:研究结核菌素(Purified Protein Derivation,PPD)皮试在诊断潜伏结核感染和结核病中的意义。方法:2008年1月~2009年12月怀疑结核感染的2714例小儿PPD皮试,结果登记并随访分析,PPD阳性率在不同年龄组间、不同性别、有无卡介苗接种的卡疤组间进行χ2检验。结果:28例皮试硬结反应出现在4~7d;潜伏结核的感染率为22.2%(603/2714),结核病为5.8%(158/2714),卡介苗病为0.3%(8/2714);PPD+^+++的阳性率在各年龄组间、有无卡疤组间比较差异有统计学意义,在性别间比较差异无统计学意义。结论:因PPD皮试安全且不良反应小推荐为常规的结核病筛查,结果观察时间建议为3~5d;皮试的颜色与肿胀边界在判断结核感染上更有意义。展开更多
基金supported by the National Natural Science Foundation(81871691)the Beijing Municipal Natural Science Foundation(KZ202110025034).
文摘Dear Editor,The tuberculin skin test(TST)reagents have continuously improved,with the ESAT6-CFP10(EC)test having recently been introduced,but are seldom based on the direction of the delayed-type hypersensitivity(DTH)mechanism.Previous studies only partially showed the infiltration and activation of immune cells and the production of cytokines of the skin induration[1,2],and lack the detailed measurements of cell proportions and gene expression in the DTH response.Therefore,in this study,we revealed the comprehensive characteristics of DTH by single-cell RNA sequencing(scRNA-seq)in the guinea pig tuberculosis(TB)model[Experimental Animal Welfare Ethics Committee,Beijing Tuberculosis and Thoracic Tumor Research Institute(2021-064)].
文摘目的:研究结核菌素(Purified Protein Derivation,PPD)皮试在诊断潜伏结核感染和结核病中的意义。方法:2008年1月~2009年12月怀疑结核感染的2714例小儿PPD皮试,结果登记并随访分析,PPD阳性率在不同年龄组间、不同性别、有无卡介苗接种的卡疤组间进行χ2检验。结果:28例皮试硬结反应出现在4~7d;潜伏结核的感染率为22.2%(603/2714),结核病为5.8%(158/2714),卡介苗病为0.3%(8/2714);PPD+^+++的阳性率在各年龄组间、有无卡疤组间比较差异有统计学意义,在性别间比较差异无统计学意义。结论:因PPD皮试安全且不良反应小推荐为常规的结核病筛查,结果观察时间建议为3~5d;皮试的颜色与肿胀边界在判断结核感染上更有意义。
