Previous study indicated that the thermo-sensitive genic malesterile(TGMS) gene in rice was regulated by temperature.TGMS rice plays an important role in hybrid rice production,because the application of the TGMS syst...Previous study indicated that the thermo-sensitive genic malesterile(TGMS) gene in rice was regulated by temperature.TGMS rice plays an important role in hybrid rice production,because the application of the TGMS system in two-line breeding is laborsaving,timesaving,simple,inexpensive,efficient,and eliminating the limitations of the cytoplasmic male sterility(CMS) system.'AnnongS' is the first discovered and deeply studied TGMS rice lines in China.'AnnongS-1' and 'Y58S',two derivatives of TGMS line AnnongS,were both controlled by a single recessive gene named tms5,which was genetically mapped on chromosome 2.In this study,three populations('AnnongS-1' × 'Nanjing11','Y58S' × 'Q611',and 'Y58S' × 'Guanghui122') were developed and used for the molecular fine mapping of the tms5 gene.By analyzing recombination events in the sterile individuals using a total of 125 probes covering the tms5 region,the tms5 gene was physically mapped to a 19-kb DNA fragment between two markers 4039-1 and 4039-2,which were located on the BAC clone AP004039.After the construction of the physical map between two markers 4039-1 and 4039-2,a member(ONAC023) of the NAC(NAM-ATAF-CUC-related) gene family was identified as the candidate gene of the tms5 gene.展开更多
为创制更丰富的豌豆变异材料,获取优异突变体豌豆种质,该研究以‘青建1号’豌豆为试验材料,以甲基磺酸乙酯(EMS)作为诱变剂,以EMS浓度1%、诱变时间8 h为半致死诱变条件,分析该诱变条件下豌豆植株突变类型,获得突变体重要表型性状数据,...为创制更丰富的豌豆变异材料,获取优异突变体豌豆种质,该研究以‘青建1号’豌豆为试验材料,以甲基磺酸乙酯(EMS)作为诱变剂,以EMS浓度1%、诱变时间8 h为半致死诱变条件,分析该诱变条件下豌豆植株突变类型,获得突变体重要表型性状数据,建立豌豆表型突变体库,并结合田间表型数据,筛选优异突变体材料。结果表明:(1)通过1%、8 h EMS诱变10000粒豌豆种子,M_(1)群体有4682株成苗,M_(2)群体筛选到342份豌豆突变体。(2)突变体豌豆表型性状突变类型比较丰富,其中单株籽粒干重变异系数最大,达到0.965。(3)通过对田间表型数据进行综合分析,筛选到10份优异的豌豆突变体种质。该研究结果丰富了豌豆种质资源,可为豌豆相关功能基因挖掘和研究及优良品种选育提供参考依据。展开更多
目的:通过基因型和表型的关联性研究,探究B基因c.917T>C变异(p.L306P)对B型糖基转移酶(GTB)表达及功能的影响,以阐明这一亚型发生的分子机制。方法:研究对象是一例初筛正反定型不一致的献血者标本,利用ABO血型血清学鉴定、GTB活性检...目的:通过基因型和表型的关联性研究,探究B基因c.917T>C变异(p.L306P)对B型糖基转移酶(GTB)表达及功能的影响,以阐明这一亚型发生的分子机制。方法:研究对象是一例初筛正反定型不一致的献血者标本,利用ABO血型血清学鉴定、GTB活性检测,鉴定ABO表型;随后采用Sanger测序和基于PacBio平台的三代测序技术对ABO基因进行测序,得到单倍体序列,比对发现突变位点;建立细胞体外表达系统,分析突变位点对于抗原表达的影响。结果:本例先证者为B亚型,等位基因型为c.917T>C in ABO*B.01/ABO*O.01.01,尚未见既往报道。体外表达结果显示,突变型细胞的GTB表达量降低、GTB总体活性降低,突变型细胞膜表面B抗原弱于野生型。结论:由ABO*B.01等位基因中c.917T>C突变所引起的p.L306P变异,可能是导致红细胞表面B抗原减少的遗传学原因。展开更多
干旱是影响玉米生长发育的重要因素,挖掘玉米抗旱关键基因,对玉米抗旱遗传改良具有重要意义。利用EMS诱变自交系RP125获得了卷叶突变体swl5(sensitive to water loss 5),在田间干旱条件下swl5呈现明显的叶片失水卷曲表型。图位克隆将候...干旱是影响玉米生长发育的重要因素,挖掘玉米抗旱关键基因,对玉米抗旱遗传改良具有重要意义。利用EMS诱变自交系RP125获得了卷叶突变体swl5(sensitive to water loss 5),在田间干旱条件下swl5呈现明显的叶片失水卷曲表型。图位克隆将候选基因定位到5号染色体280 kb的物理区域内,该区间有9个基因,其中GRMZM2G002260基因第748位碱基处发生了C→T的突变,翻译提前终止,该基因为已报道的ZmWI5,编码一个GH10家族的内源-1,4-木聚糖酶。为进一步验证基因功能,获得了ZmWI5的突变体wi5,swl5与wi5突变体杂交F_(1)表现与swl5、wi5相同的叶片卷曲失水表型。干旱胁迫下,swl5突变体的净光合速率、气孔导度、蒸腾速率较对照RP125显著降低,叶片温度明显升高,H_(2)O_(2)含量、O_(2)^(-)含量显著升高。与RP125相比,突变体中参与纤维素合成的基因CESA5及木聚糖合成的基因DUF579、GT47、IRX9、IRX9H-1、IRX9H-2、GUX1、TBL33表达量在正常浇水和干旱处理下均显著下调。综上表明,swl5是ZmWI5的一个新等位突变体,swl5突变体表现明显的旱敏感表型,且突变体中木聚糖合成相关基因表达显著降低。展开更多
文摘Previous study indicated that the thermo-sensitive genic malesterile(TGMS) gene in rice was regulated by temperature.TGMS rice plays an important role in hybrid rice production,because the application of the TGMS system in two-line breeding is laborsaving,timesaving,simple,inexpensive,efficient,and eliminating the limitations of the cytoplasmic male sterility(CMS) system.'AnnongS' is the first discovered and deeply studied TGMS rice lines in China.'AnnongS-1' and 'Y58S',two derivatives of TGMS line AnnongS,were both controlled by a single recessive gene named tms5,which was genetically mapped on chromosome 2.In this study,three populations('AnnongS-1' × 'Nanjing11','Y58S' × 'Q611',and 'Y58S' × 'Guanghui122') were developed and used for the molecular fine mapping of the tms5 gene.By analyzing recombination events in the sterile individuals using a total of 125 probes covering the tms5 region,the tms5 gene was physically mapped to a 19-kb DNA fragment between two markers 4039-1 and 4039-2,which were located on the BAC clone AP004039.After the construction of the physical map between two markers 4039-1 and 4039-2,a member(ONAC023) of the NAC(NAM-ATAF-CUC-related) gene family was identified as the candidate gene of the tms5 gene.
文摘为创制更丰富的豌豆变异材料,获取优异突变体豌豆种质,该研究以‘青建1号’豌豆为试验材料,以甲基磺酸乙酯(EMS)作为诱变剂,以EMS浓度1%、诱变时间8 h为半致死诱变条件,分析该诱变条件下豌豆植株突变类型,获得突变体重要表型性状数据,建立豌豆表型突变体库,并结合田间表型数据,筛选优异突变体材料。结果表明:(1)通过1%、8 h EMS诱变10000粒豌豆种子,M_(1)群体有4682株成苗,M_(2)群体筛选到342份豌豆突变体。(2)突变体豌豆表型性状突变类型比较丰富,其中单株籽粒干重变异系数最大,达到0.965。(3)通过对田间表型数据进行综合分析,筛选到10份优异的豌豆突变体种质。该研究结果丰富了豌豆种质资源,可为豌豆相关功能基因挖掘和研究及优良品种选育提供参考依据。
文摘目的:通过基因型和表型的关联性研究,探究B基因c.917T>C变异(p.L306P)对B型糖基转移酶(GTB)表达及功能的影响,以阐明这一亚型发生的分子机制。方法:研究对象是一例初筛正反定型不一致的献血者标本,利用ABO血型血清学鉴定、GTB活性检测,鉴定ABO表型;随后采用Sanger测序和基于PacBio平台的三代测序技术对ABO基因进行测序,得到单倍体序列,比对发现突变位点;建立细胞体外表达系统,分析突变位点对于抗原表达的影响。结果:本例先证者为B亚型,等位基因型为c.917T>C in ABO*B.01/ABO*O.01.01,尚未见既往报道。体外表达结果显示,突变型细胞的GTB表达量降低、GTB总体活性降低,突变型细胞膜表面B抗原弱于野生型。结论:由ABO*B.01等位基因中c.917T>C突变所引起的p.L306P变异,可能是导致红细胞表面B抗原减少的遗传学原因。
文摘干旱是影响玉米生长发育的重要因素,挖掘玉米抗旱关键基因,对玉米抗旱遗传改良具有重要意义。利用EMS诱变自交系RP125获得了卷叶突变体swl5(sensitive to water loss 5),在田间干旱条件下swl5呈现明显的叶片失水卷曲表型。图位克隆将候选基因定位到5号染色体280 kb的物理区域内,该区间有9个基因,其中GRMZM2G002260基因第748位碱基处发生了C→T的突变,翻译提前终止,该基因为已报道的ZmWI5,编码一个GH10家族的内源-1,4-木聚糖酶。为进一步验证基因功能,获得了ZmWI5的突变体wi5,swl5与wi5突变体杂交F_(1)表现与swl5、wi5相同的叶片卷曲失水表型。干旱胁迫下,swl5突变体的净光合速率、气孔导度、蒸腾速率较对照RP125显著降低,叶片温度明显升高,H_(2)O_(2)含量、O_(2)^(-)含量显著升高。与RP125相比,突变体中参与纤维素合成的基因CESA5及木聚糖合成的基因DUF579、GT47、IRX9、IRX9H-1、IRX9H-2、GUX1、TBL33表达量在正常浇水和干旱处理下均显著下调。综上表明,swl5是ZmWI5的一个新等位突变体,swl5突变体表现明显的旱敏感表型,且突变体中木聚糖合成相关基因表达显著降低。
