To perform the mechanism study of special association for vancomycin and D-Ala-D-Ala-containing peptides on the interface of solution and self-assemble monolayer, the binding between vancomycin and pentapeptide （Lys-...To perform the mechanism study of special association for vancomycin and D-Ala-D-Ala-containing peptides on the interface of solution and self-assemble monolayer, the binding between vancomycin and pentapeptide （Lys-Lys-Gly-D-Ala-D-Ala） was investigated by flow injection surface plasmon resonance （FI-SPR） and flow injection quartz crystal microbalance （FI-QCM）. To facilitate the formation of a compact vancomycin adsorbates layer with a uniform surface orientation, vancomycin molecules were attached onto a preformed alkanethiol self-assembled monolayer. By optimizing the conditions for the binding between Lys-Lys-Gly-D-Ala-D-Ala and vancomycin on the assembled chip, the detecting limit of Lys-Lys-Gly-D-Ala-D-Ala was greatly improved （reaching 0.5 ×10^- 6 mol/L or 7.5 × 10^-12 mol）. The equilibrium constant of the association of Lys-Lys-Gly-D-Ala-D-Ala with vancomycin was also obtained （KAds=5.0×10^4 L/tool）.展开更多
A high spatial resolution, phase-sensitive Surface Plasmon Resonance(SPR) sensor based on Extraordinary Optical Transmission(EOT) is proposed to monitor the binding of organic and biological molecules to the silver su...A high spatial resolution, phase-sensitive Surface Plasmon Resonance(SPR) sensor based on Extraordinary Optical Transmission(EOT) is proposed to monitor the binding of organic and biological molecules to the silver surface. The 2D nanohole-array configuration is well suited for dense integration in a sensor chip. The optical geometry is collinear, which simplifies the alignment with respect to the traditional Kretschmann arrangement for SPR sensing. Various design parameters of the device have been studied by simulation. The heterodyne technique is used to improve the sensitivity. The optimization results indicate that the sensor has the advantages of achieving high resolution and a wide dynamic range simultaneously.展开更多
Surface-enhanced resonance Raman scattering(SERRS)has recently attracted great interest in analytical science due toenormous enhancement factors that have decreased the detec-tionli mits of a wide variety of molecules...Surface-enhanced resonance Raman scattering(SERRS)has recently attracted great interest in analytical science due toenormous enhancement factors that have decreased the detec-tionli mits of a wide variety of molecules to the single moleculelevel.The SERRS-electromagnetic(EM)model describessingle-molecule SERRS展开更多
基金Projects(20773165,20876179) supported by the National Natural Science Foundation of ChinaProject(09JJ1002) supported by the Hunan Provincial Natural Science Foundation,China+1 种基金Project(NCET-07-0865) for New Century Excellent Talents in Chinese UniversityProject(2007AA022006) supported by the National High Technology Research and Development Program of China
文摘To perform the mechanism study of special association for vancomycin and D-Ala-D-Ala-containing peptides on the interface of solution and self-assemble monolayer, the binding between vancomycin and pentapeptide （Lys-Lys-Gly-D-Ala-D-Ala） was investigated by flow injection surface plasmon resonance （FI-SPR） and flow injection quartz crystal microbalance （FI-QCM）. To facilitate the formation of a compact vancomycin adsorbates layer with a uniform surface orientation, vancomycin molecules were attached onto a preformed alkanethiol self-assembled monolayer. By optimizing the conditions for the binding between Lys-Lys-Gly-D-Ala-D-Ala and vancomycin on the assembled chip, the detecting limit of Lys-Lys-Gly-D-Ala-D-Ala was greatly improved （reaching 0.5 ×10^- 6 mol/L or 7.5 × 10^-12 mol）. The equilibrium constant of the association of Lys-Lys-Gly-D-Ala-D-Ala with vancomycin was also obtained （KAds=5.0×10^4 L/tool）.
基金Supported by the funding from the Hong Kong Research Grants Council under CERG project 411907 and41228National Basis Research Program of China(973)(No.2009CB930600)
文摘A high spatial resolution, phase-sensitive Surface Plasmon Resonance(SPR) sensor based on Extraordinary Optical Transmission(EOT) is proposed to monitor the binding of organic and biological molecules to the silver surface. The 2D nanohole-array configuration is well suited for dense integration in a sensor chip. The optical geometry is collinear, which simplifies the alignment with respect to the traditional Kretschmann arrangement for SPR sensing. Various design parameters of the device have been studied by simulation. The heterodyne technique is used to improve the sensitivity. The optimization results indicate that the sensor has the advantages of achieving high resolution and a wide dynamic range simultaneously.
文摘Surface-enhanced resonance Raman scattering(SERRS)has recently attracted great interest in analytical science due toenormous enhancement factors that have decreased the detec-tionli mits of a wide variety of molecules to the single moleculelevel.The SERRS-electromagnetic(EM)model describessingle-molecule SERRS
