PM2 gene （accession number： M80664） with high lysine content from soybean （Glycine max） was found in GenBank by changing three BLASTp parameters. Amino acid composition analysis of PM2 showed that Lys content was...PM2 gene （accession number： M80664） with high lysine content from soybean （Glycine max） was found in GenBank by changing three BLASTp parameters. Amino acid composition analysis of PM2 showed that Lys content was on the high level of 18.22%. Protein encoded by PM＇2 also belonged to the family of late embryogenesis abundant （LEA） proteins, which was considered that it had a strong relation with the abiotic stress resistance. In this experiment, PM2 gene was obtained from dry soybean seeds by RT-PCR, plant expression vector pEMTPM2 was constructed, and then transformed into tobacco by using agrobacterium-mediated method. Eight salt and drought tolerant lines were obtained from 31 differentiated lines. Real-time PCR showed that PM2 gene overexpressed in all four PCR positive lines with the osmotic stress resistance. These results confirmed that the overexpression of PM2 gene enhanced the osmotic stress resistance of transgenic tobacco.展开更多
Total RNA from leaves of Poapratensis cultivars under drought stress was extracted for reversing transcription to cDNA and then cDNA as template for PCR reaction by designing primer of cds of Hordeum valgare HVA1 drou...Total RNA from leaves of Poapratensis cultivars under drought stress was extracted for reversing transcription to cDNA and then cDNA as template for PCR reaction by designing primer of cds of Hordeum valgare HVA1 drought resistance gene from GenBank. The amplified products were positive recon identified by using procedures of recovery, connection, transformation and enzyme separation. The length of cloned gene sequence was 324 bp, identity reached 79.27% with Barley HVA1 gene that meaned the cloned gene sequence was the partial HVA1 gene of Poapratensis.展开更多
The CaO-doped Cu/（NiFe2O4-10NiO） cermet inert anodes were prepared by the cold isostatie pressing-sintering process, and their corrosion resistance to Na3AlF6-K3AlF6-Al203 melt was studied. The results show that the...The CaO-doped Cu/（NiFe2O4-10NiO） cermet inert anodes were prepared by the cold isostatie pressing-sintering process, and their corrosion resistance to Na3AlF6-K3AlF6-Al203 melt was studied. The results show that the relative density of 5Cu/（NiFe2O4-10NiO） cermet sintered at 1 200 ℃ increases from 82.83% to 97.63% when 2% CaO （mass fraction） is added. During the electrolysis, the relative density of cermet inert anode descends owing to the chemical dissolution of additive CaO at ceramic grain boundary, which accelerates the penetration of electrolyte. Thus, the corrosion resistance to melts of Cu/（NiFe2O4-10NiO） cermet inert anode is reduced. To improve the corrosion resistance of the cermet inert anode, the content of CaO doped should be decreased and the technology of cleaning the ceramic grain boundary should be applied.展开更多
Aiming at determining the thermal contact resistance of ball screws,a new analytical method combining the minimum excess principle with the MB fractal theory is proposed to estimate thermal contact resistance of ball ...Aiming at determining the thermal contact resistance of ball screws,a new analytical method combining the minimum excess principle with the MB fractal theory is proposed to estimate thermal contact resistance of ball screws considering microscopic fractal characteristics of contact surfaces.The minimum excess principle is employed for normal stress analysis.Moreover,the MB fractal theory is adopted for thermal contact resistance.The effectiveness of the proposed method is validated by self-designed experiment.The comparison between theoretical and experimental results demonstrates that thermal contact resistance of ball screws can be obtained by the proposed method.On this basis,effects of fractal parameters on thermal contact resistance of ball screws are discussed.Moreover,effects of the axial load on thermal contact resistance of ball screws are also analyzed.The conclusion can be drawn that the thermal contact resistance decreases along with the fractal dimension D increase and it increases along with the scale parameter G increase,and thermal contact resistance of ball screws is retained almost constant along with axial load increase before the preload of the right nut turns into zero in value.The application of the proposed method is also conducted and validated by the temperature measurement on a self-designed test bed.展开更多
In order to study the alkali resistivity of VHA-c in Iris lacteal. （IrlVHA-c）, the transgenic tobacco seedlings harboring lrlVHA-c gene from Iris lactea （To）, the self-crossed progeny （T1）, and the non-transgeni...In order to study the alkali resistivity of VHA-c in Iris lacteal. （IrlVHA-c）, the transgenic tobacco seedlings harboring lrlVHA-c gene from Iris lactea （To）, the self-crossed progeny （T1）, and the non-transgenic lines of tobacco seedlings were grown in Hoagland nutrient solutions supplemented with 0, 100, 200, 300, 400 mmol.L^-1 NaHCO3. The MDA content, CAT, POD and SOD activity, electrical conductivity, chlorophyll content, soluble sugar content, proline content and polyphenol oxidase activity of the seedlings were determined. The results showed that the transgenic lines of tobacco maintained a high activity up to 200 mmol.L^-1 NaHCO3, and activity was slightly lower at 300 mmol.L^-1 NaHCOa. When the concentration of NaHCO3 was as high as 400 mmol.L^-1 the seedlings were badly hurt. In addition, the activity of To and T1 transgentic tobacoo was maintained more or less. While the non- transgenic lines of tobacco could maintain viably up to 100 mmol. L^-1 NaHCO3, and they could not survive at 400 mmol. L^-1 NaHCO3. The conclusion was drawn that the alkali resistance of the tobacoo transformed IrlVHA-c was noticeably improved.展开更多
他莫昔芬(tamoxifen,TAM)作为雌激素受体阳性(estrogen receptor,ER+)乳腺癌的一线化疗药物使大多数患者受益,但原发性和继发性耐药问题严重影响临床治疗效果。深入研究ER+乳腺癌TAM耐药机制,改善治疗效果是当前亟待解决的问题。抑癌因...他莫昔芬(tamoxifen,TAM)作为雌激素受体阳性(estrogen receptor,ER+)乳腺癌的一线化疗药物使大多数患者受益,但原发性和继发性耐药问题严重影响临床治疗效果。深入研究ER+乳腺癌TAM耐药机制,改善治疗效果是当前亟待解决的问题。抑癌因子NDRG2(N-myc downstream regulated gene 2,NDRG2)在肿瘤发生发展中发挥重要作用,但是否参与ER+乳腺癌TAM耐药尚不清楚。本研究旨在探明NDRG2在ER+乳腺癌TAM耐药中发挥的作用和机制。通过RT-PCR与免疫印迹分析对比TAM敏感型和耐药型ER+乳腺癌细胞发现,NDRG 2的mRNA转录水平和蛋白质翻译水平在TAM耐药细胞中表达显著下调,且与耐药能力负相关(P<0.001);CCK-8细胞毒性实验和软琼脂克隆形成实验证实,在耐药细胞中过表达NDRG2可显著降低TAM药物半抑制浓度IC 50和软琼脂克隆形成率(P<0.001),逆转耐药表型。分子机制上,X-box结合蛋白1(X-box binding protein 1,XBP1)mRNA剪切实验与内质网相关降解(endoplasmic-reticulum associated degradation,ERAD)报告蛋白的结果显示,过表达NDRG2可增强耐药细胞中剪切型XBP1s mRNA转录与ERAD报告蛋白CD3ε-YFP表达(P<0.001),引发耐药细胞内质网强应激反应;免疫印迹检测结果显示,过表达NDRG2可显著提高耐药细胞中内质网应激感受器肌醇需要激酶1α(inositol requiring enzyme 1,IRE1α)的磷酸化水平及其下游因子,例如内质网EIP辅助因子(endoplasmic reticulum-localized DnaJ 4,ERdj4)、PKR蛋白激酶的细胞抑制剂(cellular Inhibitor of the PKR protein kinase,P58 IPK)、α甘露糖苷酶样应激蛋白(er degradation enhancingαmannosidase likeprotein,EDEM)和蛋白质二硫键异构酶家族A成员5(protein disulfide isomerase family a member 5,PDIA5)的表达水平(P<0.001)。小鼠异种移植瘤研究进一步证实,在耐药细胞中过表达NDRG2可增强TAM治疗效果,显著抑制耐药移植瘤生长(P<0.001)。以上研究结果表明,通过提高耐药细胞中NDRG2表达,增强TAM治疗引发的内质网强烈应激,可逆转ER+乳腺癌细胞耐药性,改善TAM治疗效果。研究结果为解决ER+乳腺癌TAM耐药问题提供了新的思路和有价值的潜在药物靶点。展开更多
基金Supported by Key Program of Natural Science Foundation of Heilongjiang Province (ZJN03-5)
文摘PM2 gene （accession number： M80664） with high lysine content from soybean （Glycine max） was found in GenBank by changing three BLASTp parameters. Amino acid composition analysis of PM2 showed that Lys content was on the high level of 18.22%. Protein encoded by PM＇2 also belonged to the family of late embryogenesis abundant （LEA） proteins, which was considered that it had a strong relation with the abiotic stress resistance. In this experiment, PM2 gene was obtained from dry soybean seeds by RT-PCR, plant expression vector pEMTPM2 was constructed, and then transformed into tobacco by using agrobacterium-mediated method. Eight salt and drought tolerant lines were obtained from 31 differentiated lines. Real-time PCR showed that PM2 gene overexpressed in all four PCR positive lines with the osmotic stress resistance. These results confirmed that the overexpression of PM2 gene enhanced the osmotic stress resistance of transgenic tobacco.
基金Supported by Committee of Nature Science Foundation of Heilongjiang Province (C0207)
文摘Total RNA from leaves of Poapratensis cultivars under drought stress was extracted for reversing transcription to cDNA and then cDNA as template for PCR reaction by designing primer of cds of Hordeum valgare HVA1 drought resistance gene from GenBank. The amplified products were positive recon identified by using procedures of recovery, connection, transformation and enzyme separation. The length of cloned gene sequence was 324 bp, identity reached 79.27% with Barley HVA1 gene that meaned the cloned gene sequence was the partial HVA1 gene of Poapratensis.
基金Project(2005CB623703) supported by the Major State Basic Research and Development Program of ChinaProject(2008AA030503) supported by Hi-Tech Research and Development Program of China
文摘The CaO-doped Cu/（NiFe2O4-10NiO） cermet inert anodes were prepared by the cold isostatie pressing-sintering process, and their corrosion resistance to Na3AlF6-K3AlF6-Al203 melt was studied. The results show that the relative density of 5Cu/（NiFe2O4-10NiO） cermet sintered at 1 200 ℃ increases from 82.83% to 97.63% when 2% CaO （mass fraction） is added. During the electrolysis, the relative density of cermet inert anode descends owing to the chemical dissolution of additive CaO at ceramic grain boundary, which accelerates the penetration of electrolyte. Thus, the corrosion resistance to melts of Cu/（NiFe2O4-10NiO） cermet inert anode is reduced. To improve the corrosion resistance of the cermet inert anode, the content of CaO doped should be decreased and the technology of cleaning the ceramic grain boundary should be applied.
基金Projects(51875008,51505012,51575014)supported by the National Natural Science Foundation of ChinaProject supported by the China Scholarship Council
文摘Aiming at determining the thermal contact resistance of ball screws,a new analytical method combining the minimum excess principle with the MB fractal theory is proposed to estimate thermal contact resistance of ball screws considering microscopic fractal characteristics of contact surfaces.The minimum excess principle is employed for normal stress analysis.Moreover,the MB fractal theory is adopted for thermal contact resistance.The effectiveness of the proposed method is validated by self-designed experiment.The comparison between theoretical and experimental results demonstrates that thermal contact resistance of ball screws can be obtained by the proposed method.On this basis,effects of fractal parameters on thermal contact resistance of ball screws are discussed.Moreover,effects of the axial load on thermal contact resistance of ball screws are also analyzed.The conclusion can be drawn that the thermal contact resistance decreases along with the fractal dimension D increase and it increases along with the scale parameter G increase,and thermal contact resistance of ball screws is retained almost constant along with axial load increase before the preload of the right nut turns into zero in value.The application of the proposed method is also conducted and validated by the temperature measurement on a self-designed test bed.
基金Supported by the Post-doctoral research start-up funding of Heilongjiang Province (LBH-210144)Postdoctoral Funds Project of Heilongjiang Province (520-415037)
文摘In order to study the alkali resistivity of VHA-c in Iris lacteal. （IrlVHA-c）, the transgenic tobacco seedlings harboring lrlVHA-c gene from Iris lactea （To）, the self-crossed progeny （T1）, and the non-transgenic lines of tobacco seedlings were grown in Hoagland nutrient solutions supplemented with 0, 100, 200, 300, 400 mmol.L^-1 NaHCO3. The MDA content, CAT, POD and SOD activity, electrical conductivity, chlorophyll content, soluble sugar content, proline content and polyphenol oxidase activity of the seedlings were determined. The results showed that the transgenic lines of tobacco maintained a high activity up to 200 mmol.L^-1 NaHCO3, and activity was slightly lower at 300 mmol.L^-1 NaHCOa. When the concentration of NaHCO3 was as high as 400 mmol.L^-1 the seedlings were badly hurt. In addition, the activity of To and T1 transgentic tobacoo was maintained more or less. While the non- transgenic lines of tobacco could maintain viably up to 100 mmol. L^-1 NaHCO3, and they could not survive at 400 mmol. L^-1 NaHCO3. The conclusion was drawn that the alkali resistance of the tobacoo transformed IrlVHA-c was noticeably improved.
文摘他莫昔芬(tamoxifen,TAM)作为雌激素受体阳性(estrogen receptor,ER+)乳腺癌的一线化疗药物使大多数患者受益,但原发性和继发性耐药问题严重影响临床治疗效果。深入研究ER+乳腺癌TAM耐药机制,改善治疗效果是当前亟待解决的问题。抑癌因子NDRG2(N-myc downstream regulated gene 2,NDRG2)在肿瘤发生发展中发挥重要作用,但是否参与ER+乳腺癌TAM耐药尚不清楚。本研究旨在探明NDRG2在ER+乳腺癌TAM耐药中发挥的作用和机制。通过RT-PCR与免疫印迹分析对比TAM敏感型和耐药型ER+乳腺癌细胞发现,NDRG 2的mRNA转录水平和蛋白质翻译水平在TAM耐药细胞中表达显著下调,且与耐药能力负相关(P<0.001);CCK-8细胞毒性实验和软琼脂克隆形成实验证实,在耐药细胞中过表达NDRG2可显著降低TAM药物半抑制浓度IC 50和软琼脂克隆形成率(P<0.001),逆转耐药表型。分子机制上,X-box结合蛋白1(X-box binding protein 1,XBP1)mRNA剪切实验与内质网相关降解(endoplasmic-reticulum associated degradation,ERAD)报告蛋白的结果显示,过表达NDRG2可增强耐药细胞中剪切型XBP1s mRNA转录与ERAD报告蛋白CD3ε-YFP表达(P<0.001),引发耐药细胞内质网强应激反应;免疫印迹检测结果显示,过表达NDRG2可显著提高耐药细胞中内质网应激感受器肌醇需要激酶1α(inositol requiring enzyme 1,IRE1α)的磷酸化水平及其下游因子,例如内质网EIP辅助因子(endoplasmic reticulum-localized DnaJ 4,ERdj4)、PKR蛋白激酶的细胞抑制剂(cellular Inhibitor of the PKR protein kinase,P58 IPK)、α甘露糖苷酶样应激蛋白(er degradation enhancingαmannosidase likeprotein,EDEM)和蛋白质二硫键异构酶家族A成员5(protein disulfide isomerase family a member 5,PDIA5)的表达水平(P<0.001)。小鼠异种移植瘤研究进一步证实,在耐药细胞中过表达NDRG2可增强TAM治疗效果,显著抑制耐药移植瘤生长(P<0.001)。以上研究结果表明,通过提高耐药细胞中NDRG2表达,增强TAM治疗引发的内质网强烈应激,可逆转ER+乳腺癌细胞耐药性,改善TAM治疗效果。研究结果为解决ER+乳腺癌TAM耐药问题提供了新的思路和有价值的潜在药物靶点。
