Objective:Ovarian cancer(OC)ranks among the leading causes of mortality among the female cancers worldwide.Numerous studies have explored the development and progression of OC at multiple genetic regulatory levels.How...Objective:Ovarian cancer(OC)ranks among the leading causes of mortality among the female cancers worldwide.Numerous studies have explored the development and progression of OC at multiple genetic regulatory levels.However,relatively few studies have explored the impact of post-translational modifications(PTM)on OC progression,which is essential for uncovering new therapeutic targets.This study aimed to systematically identify the key PTM types involved in OCprogression,and to explore and evaluate their translational potential as therapeutic targets.Methods:First,we utilized multiple general PTM antibodies to compare gross PTM levels between normal ovarian and OC tissues from clinical females.After identifying lactylation as the PTM with the most significant differences,we selected representative samples for label-free mass spectrometry to identify specific lactylation sites.Next,we transfected A2780(OC)cells with either wild-type(WT)or mutant(K192A[Q])poly(ADP-ribose)polymerase 1(PARP1)conjugated to enhanced green fluorescent protein(EGFP)with a StrepⅡpeptide tag and assessed various cellular indexes related to cell proliferation(clonogenicity assay),migration(scratch wound healing assay),and reactive oxygen species levels.Results:Pan-lactylation was significantly upregulated in clinical OC samples,with PARP1 lactylation at K192 being one of the most common modifications.The growth and migration of A2780 cells were markedly suppressed by overexpressing PARP1-WT but not mutant PARP1.Overexpressing PARP1 significantly downregulated the phosphorylation of extracellular signal-regulated kinases 1/2(ERK1/2).Conclusion:This study uncovered a novel PTM of PARP1 in OC,lactylation,and demonstrated that lactylation at K192 is crucial in regulating OC cell growth and migration via the ERK1/2 pathway.Further investigations are required to elucidate the broader functional implications of PARP1 lactylation and its therapeutic potential.展开更多
During the storage of composite propellants, the migration of plasticizers and other unbonded additives at the interfaces of liner adhesives has garnered significant attention in understanding liner failure mechanisms...During the storage of composite propellants, the migration of plasticizers and other unbonded additives at the interfaces of liner adhesives has garnered significant attention in understanding liner failure mechanisms, aging processes, and safety performance. However, there is currently no non-destructive and quantitative detection method for migration of plasticizers in propellant liner. In this study, we developed a HTPB sensing liner by incorporating conductive fillers-namely carbon black(CB), carbon nanotubes(CNTs), and graphene nanoplatelets(GNP)-into the HTPB matrix. The synergistic interaction between CNTs and GNP facilitates the formation of a tunneling conductive network that imparts electrical conductivity to the HTPB liner. To elucidate the functional relationship between conductivity and nitroglycerin(NG) migration, we applied the HTPB sensing liner onto double base propellant surfaces and measured both the conductivity of the sensing layer and NG migration during a 71°C accelerated aging experiment. The results shows that when CNTs/GNP content reaches 3wt%, there is an exponential correlation between conductivity and NG migration with a fitting degree of 0.9652;the average response sensitivity of ΔR/R0 relative to NG migration is calculated as 41.69, with an average deviation of merely5.67% between NG migrations derived from conductivity fittings compared to those obtained via TGA testing results. Overall, this sensing liner exhibits excellent capabilities for detecting NG migration nondestructively and quantitatively while offering a novel approach for assessing interfacial component migrations as well as debonding defects in propellants-a promising avenue for future self-monitoring strategies regarding propellant integrity.展开更多
The detection of hypersonic targets usually confronts range migration(RM)issue before coherent integration(CI).The traditional methods aiming at correcting RM to obtain CI mainly considers the narrow-band radar condit...The detection of hypersonic targets usually confronts range migration(RM)issue before coherent integration(CI).The traditional methods aiming at correcting RM to obtain CI mainly considers the narrow-band radar condition.However,with the increasing requirement of far-range detection,the time bandwidth product,which is corresponding to radar’s mean power,should be promoted in actual application.Thus,the echo signal generates the scale effect(SE)at large time bandwidth product situation,influencing the intra and inter pulse integration performance.To eliminate SE and correct RM,this paper proposes an effective algorithm,i.e.,scaled location rotation transform(ScLRT).The ScLRT can remove SE to obtain the matching pulse compression(PC)as well as correct RM to complete CI via the location rotation transform,being implemented by seeking the actual rotation angle.Compared to the traditional coherent detection algorithms,Sc LRT can address the SE problem to achieve better detection/estimation capabilities.At last,this paper gives several simulations to assess the viability of ScLRT.展开更多
Lateral migration of fault activity in Weihe basin is a popular phenomenon and its characteristics are also typical. Taking the activity migrations of Wangshun Mountain piedmont fault toward Lishan piedmont fault and ...Lateral migration of fault activity in Weihe basin is a popular phenomenon and its characteristics are also typical. Taking the activity migrations of Wangshun Mountain piedmont fault toward Lishan piedmont fault and Weinan platform front fault, Dabaopi-Niujiaojian fault toward Shenyusi-Xiaojiazhai fault, among a serial of NE-trending faults from Baoji city to Jingyang County as examples, their migration time and process are analyzed and discussed in the present paper. It is useful for further understanding the structure development and physiognomy evolution history of Weihe basin.展开更多
Based on arbitrarily wide-angle wave equations,a reverse-time propagation scheme is developed by substituting the partial derivatives of depth and time with central differences. The partial derivative of horizontal di...Based on arbitrarily wide-angle wave equations,a reverse-time propagation scheme is developed by substituting the partial derivatives of depth and time with central differences. The partial derivative of horizontal direction is replaced with high order difference. The imaging condition is computed by solving the eikonal equations. On the basis of above techniques,a prestack reverse-time depth migration algorithm is developed. The processing exam-ples of synthetic data show that the method can remove unwanted internal reflections and decrease the migration noise. The method also has the advantage of fidelity and is applicable of dip angle reflector imaging.展开更多
OBJECTIVE To investigate the role and mechanism of G protein-coupled receptor kinase 2(GRK2)involving in hepatocel ular carcinoma(HCC)progression.METHODS Cel Counting Kit 8 and tumor colony formation assay were design...OBJECTIVE To investigate the role and mechanism of G protein-coupled receptor kinase 2(GRK2)involving in hepatocel ular carcinoma(HCC)progression.METHODS Cel Counting Kit 8 and tumor colony formation assay were designed to detect HCC cell proliferation,wound healing assay was to detect HCC migration.The correlation between GRK2 and early growth response-1(EGR1)were detected by RT-PCR and real-time PCR assays.Co-immunoprecipitation and Western blot assay were adopted to detect the relationship between GRK2and insulin-like growth factor 1 receptor(IGF-1R)signaling pathway.RESULTS In this study we find that GRK2plays an inhibition role in IGF1-induced HCC cell proliferation and migration.Overexpression of GRK2 causes a decrease in EGR1 expression,while knockdown of GRK2 leads to the dramatically increase in EGR1 expression in the treatment of IGF1.Through co-immunoprecipitation and Western blot assay,we confirm that GRK2can interact with IGF-1R and inhibiting IGF1-induced activation of IGF1R signaling pathway.Silencing EGR1attenuates GRK2 overexpression-caused inhibition of cell proliferation,tumor colony number and migrationactivity,while overexpressing of EGR1 restores the antiproliferative and migratory effect by GRK2 overexpression in HCCLM3 cells.CONCLUSION Taken together,these results suggest that GRK2 may inhibit IGF1-induced HCC cell growth and migration through down-regulation of EGR1 and indicate that enforced GRK2 may offer a potential therapeutic approach against HCC.展开更多
The interactions on gold active and migratory quantities and rates between tuffaceous slate and solu tions with different compositions were experimentally studied at 200 ℃, 20 MPa, in a high pressure apparatus. After...The interactions on gold active and migratory quantities and rates between tuffaceous slate and solu tions with different compositions were experimentally studied at 200 ℃, 20 MPa, in a high pressure apparatus. After reaction, tuffaceous slate became light colored and soft, and its mass density reduced. The amount of gold extracted from tuffaceous slate ranges widely, from 0 027 to 0 234 μg/g. Chlorine solution may activate appreciable amount of gold, and the gold migratory rate is high enough, from 50 70% to 92 30%, which reveals that sulphur and chlorine work together in solutions to accelerate gold activation and migration, and to realize gold mineralization in favorable places.展开更多
The wind effects on steady-state scan characteristics and hit probability of terminal-sensitive projectile were discussed in this paper. Considering wind as the constitutions of the average wind and the impulsive wind...The wind effects on steady-state scan characteristics and hit probability of terminal-sensitive projectile were discussed in this paper. Considering wind as the constitutions of the average wind and the impulsive wind, a simplified wind field model was established for the ballistic calculation of the steady-state scan phase; under the windy condition, the effects of the range wind and the beam wind on the steady-state scan characteristics of the terminal-sensitive projectile were analyzed in detail and its hit probabilities for a certain armored target were calculated. The calculated results show that, when the wind speed exceeds a certain value, the hit probabilities of terminal-sensitive projectile drop rapidly; the wind effects must be considered in the application of the terminal-sensitive projectiles. This paper provides some theoretical references for the fire wind speed correction and the global structure optimization of the terminal-sensitive projectile.展开更多
Three observation methods were used to investigate the existing form and the behavior of rare earth during the sintering process of high activity mischmetal （RE, with lanthanum and cerium） doped WC-8%Co-0.048%RE（ma...Three observation methods were used to investigate the existing form and the behavior of rare earth during the sintering process of high activity mischmetal （RE, with lanthanum and cerium） doped WC-8%Co-0.048%RE（mass fraction） alloy with low carbon-containing level by scanning electron microscopy （SEM） and energy dispersive X-ray spectroscopy （EDXS）, considering the fact that the addition amount of rare earth in the alloy is very minute. The directional migration process and mechanism of cerium were discussed. First, the sinter skin （surface） is observed. oxide on the sinter skin, and lanthanum in these cerium observed, and lanthanum containing phase/micro-zone in It is shown that there exists a dispersedly distributed cerium containing enrichment positions is very minute. Secondly, the polished section is the alloy is identified. Finally, based on the fact that the fracture of cemented carbide is resulted from the heterogeneous phase or other defects within the microstructure, the fracture surface is observed and cerium containing phase/micro-zone in the fracture source approximately 260 μm from the surface is identified. These combined observations reveal adequately the fact that lanthanum and cerium get separated and cerium predominantly migrates towards the surface during the sintering process.展开更多
The migration mode transition of cancer cell enhances its invasive capability and the drug resistance,where physical confinement of cell microenvironment has been revealed to induce the mesenchymal-amoeboid transition...The migration mode transition of cancer cell enhances its invasive capability and the drug resistance,where physical confinement of cell microenvironment has been revealed to induce the mesenchymal-amoeboid transition(MAT).However,most existing studies are performed in PDMS microchannels,of which the stiffness is much higher than that of most mammalian tissues.Therefore,the amoeboid migration transition observed in these studies is actually induced by the synergistic effect of matrix stiffness and confinement.Since the stiffness of cell microenvironment has been reported to influence the cell migration in 2D substrate,the decoupling of stiffness and confinement effects is thus in need for elucidating the underlying mechanism of MAT.However,it is technically challenging to construct microchannels with physiologically relevant stiffness and channel size,where existing microchannel platforms with physiological relevance stiffness are all with>10μm channel width.Such size is too wide to mimic the physical confinement that migrating cancer cells confront in vivo,and also larger than the width of PDMS channel,in which the MAT of cancer cell was observed.Therefore,an in vitro cell migration platform,which could mimic both stiffness and confinement of the native physical microenvironment during cancer metastasis,could profoundly contribute to researches on cancer cell migration and cellular mechanotransduction.In this paper,we overcome the limitations of engineering soft materials in microscale by combining the collagen-alginate hydrogel with photolithography.This enables us to improve the accuracy of molded microchannel,and thus successfully construct a 3D microchannel platform,which matches the stiffness and width ranges of native environmental confinement that migrating cancer cells confront in vivo.The stiffness(0.3~20 kPa),confinement(channel width:3.5~14μm)and the adhesion ligand density of the microchannel can be tuned independently.Interestingly,using this platform,we observed that the migration speed of cancer cell is influenced by the synergistic effect of channel stiffness and width,and the increasing stiffness reverses the effect of channel width on the migration speed of cancer cells.In addition,MAT has a strong correlation with the channel stiffness.These findings make us reconsider the widely accepted hypothesis:physical confinement can induce MAT.Actually,this transition can only occur in stiff confined microenvironment not in soft one.For soft microchannels,the compliance of the channel walls could cause little cell/nucleus deformation,and the MAT could not be induced.To further investigate the mechanism of MAT,we developed a computational model to simulate the effect of nucleus deformation on MAT.With the model,we found that deforming the cell nuclear by decreasing the nucleus stiffness will reduce the cellmigration speed.This implies that nuclear stiffness plays an important role in the regulation of cancer migration speed and thus MAT in microchannels.The effect of channel stiffness on MAT and migration speed as observed in our experiment could partially explain previous findings reported in the literature,where the increasing matrix stiffness of tumor microenvironment promotes cancer metastasis.Our observations thus highlight the critical role of cell nuclear deformation not only in MAT,but also in regulating cellular mechanotransduction and cell-ECM interactions.This developed platform is capable of mimicking the native physical microenvironment during metastasis,providing a powerful tool for high-throughput screening applications and investigating the interaction between cancer migration and biophysical microenvironment.展开更多
The purpose of this study was to explore the effects of recombinant human intestinal alkaline phosphatase(recIAP) on human neutrophils in vitro, and the migration, phagocytosis, apoptosis in presence and absence of LP...The purpose of this study was to explore the effects of recombinant human intestinal alkaline phosphatase(recIAP) on human neutrophils in vitro, and the migration, phagocytosis, apoptosis in presence and absence of LPS. In this study, freshly extracted human neutrophils were used to establish an inflammatory cell model, and the control group, recIAP group, LPS group and recIAP +LPS group were set up to stimulate the model. The migration of neutrophils was detected by agarose gel drop method. Fluorescent particles and fluorescent probes were added to different treatment groups, and the phagocytic rate of neutrophils and the release of reactive oxygen species(ROS) from neutrophils were detected by flow cytometry. The apoptosis rate of neutrophils was detected by flow cytometry according to Annexin V-FITC apoptosis detection kit. The results showed that regardless of the presence or absence of LPS, recIAP could inhibit the migration of neutrophils, phagocytosis and the release of ROS. In addition, recIAP could weaken the inhibitory effect of LPS on neutrophils apoptosis.展开更多
The oblique transfer zone in the Fushan Sag, a syndepositional dome sandwiched between the Bailian and Huangtong sub-sags, has been the most important exploration target. The major oil observation occurs in the E_2l_1...The oblique transfer zone in the Fushan Sag, a syndepositional dome sandwiched between the Bailian and Huangtong sub-sags, has been the most important exploration target. The major oil observation occurs in the E_2l_1^(L+M) and the E_2l_3~U. 46 oil and rock samples reveal that the oil in the transfer zone is mostly contributed by the Bailian sub-sag, though the source rock conditions, hydrocarbon generation and expulsion histories of the Bailian and Huangtong sub-sags are similar. The E_2l_3~U oil, characterized by high maturity, Pr/Ph ratio and oleanane/C_(30)-hopane ratio, shows a close genetic affinity with the E_2l_3~b source rocks, while the E_2l_1^(L+M) oil, characterized by lower maturity, Pr/Ph ratio and oleanane/C_(30)-hopane ratio, is suggested to be derived from the E_2l_(1+2)~b source rocks. The homogenization temperatures of aqueous fluid inclusions, taking the burial history of the reservoirs into account, reflect that the oil charge mainly occurred from mid-Miocene to Pliocene in the oblique transfer zone. The oil transporting passages include connected sand bodies, unconformities and faults in the Fushan Sag. Of these, the faults are the most complicated and significant. The faults differ sharply in the west area, the east area and the oblique transfer zone, resulting in different influence on the oil migration and accumulation. During the main hydrocarbon charge stage, the faults in the west area are characterized by bad vertical sealing and spatially dense distribution. As a result, the oil generated by the Huangtong source rocks is mostly lost along the faults during the vertical migration in the west area. This can be the mechanism proposed to explain the little contribution of the Huangtong source rocks to the oil in the oblique transfer zone. Eventually, an oil migration and accumulation model is built in the oblique transfer zone, which may provide theoretical and practical guides for the oil exploration.展开更多
OBJECTIVE This study was aimed to evaluate the effect of mangosteen peel extract(MPE)on migration of human umbilical vein endothelial cells(HUVECs)treated with LPS.METHODS Cultured HUVECs were divided into five groups...OBJECTIVE This study was aimed to evaluate the effect of mangosteen peel extract(MPE)on migration of human umbilical vein endothelial cells(HUVECs)treated with LPS.METHODS Cultured HUVECs were divided into five groups:normal(untreated)group,positive control group treated with LPS at dose of 20ng·mL-1 for 24 h,and groups pre-treated with LPS at dose of 20ng·mL-1 for 24 h followed by incubation with MPE at doses of 1,2,and 4-1μg·mL,respectively.HUVECs migration was evaluated using wound-healing migration assay after 24 h of treatment.RESULTS LPS significantly(P<0.05)impaired HUVECs migration compared to the normal.MPE at dose of 1μg·mL-1 increased migration of LPS-treated HUVECs significantly(P<0.05)compared to the positive control and near to the normal level.CONCLUSION The mangosteen peel extract is able to dose-dependently preserve the endothelial cells function.展开更多
A coupled heat and formaldehyde migration model based on the non-equilibrium thermodynamic theory and molecule movement theory was developed.The effect of temperature on the transport coefficients was simulated,and th...A coupled heat and formaldehyde migration model based on the non-equilibrium thermodynamic theory and molecule movement theory was developed.The effect of temperature on the transport coefficients was simulated,and the simulation results were validated with experimental data from the literatures.The calculation shows that air exchange rate larger than 2 h-1 should be prevented,if the purpose is only for formaldehyde emissions control.The effects of temperature on formaldehyde migration are obvious.展开更多
OBJECTIVE Prostate cancer is the most commonly diagnosed malignancy and the sixth leading cause of cancer death in men.Aberrant upregulation of enhancer of Zeste homolog2(EZH2)occur frequently in human cancers,includi...OBJECTIVE Prostate cancer is the most commonly diagnosed malignancy and the sixth leading cause of cancer death in men.Aberrant upregulation of enhancer of Zeste homolog2(EZH2)occur frequently in human cancers,including prostate adenocarcinoma,it′s a bona fide oncogene,yet clinical benefits of EZH2 inhibitor(EZH2i)remain unsatis⁃factory and limited to certain hematological malignancies.AD20 is a natural product which is extracted from creosote bush.Our previous study has demonstrated that AD20 could inhibit the growth and metastasis in prostate cancer by inhibiting NRP1,however,the mechanisms still need to be clarified.METHODS PC3 cell were cultured in DMEM/F12 supplemented with 10%fetal bovine serum.The proliferation and migration were measured by MTS,wound healing assay and tran⁃swell assay.The expression of the protein was observed by Western blot.The mRNA levels were detected by real-time PCR.LC-MS/MS-based proteomic assay,CoIP-MS and network pharmacology are used to construct the PPI network and identify related proteins.RESULTS AD20(10-20μmol·L^-1)can regulate EZH2 expression from transcription,trans⁃lation,degradation levels.AD20 downregulates EZH2-H3K27me3 signaling dramatically and activates many down⁃stream tumor suppressor genes,such as CDH1,GATA6,CDKN2A.In vitro,AD20(10-40μmol·L^-1)suppresses PC3 cell proliferation and migration in a dose-dependent manner.And interestingly,the combination with AD20 significantly improved the efficacy of EZH2 inhibitors(GSK126 and EPZ-6438)in PC3 cell line.CONCLUSION AD20 can suppress PC3 cell proliferation by targeting EZH2-H3K27me3-p16INK4a signaling and inhibit PC3 cell migration by inhibiting NRP1.展开更多
Bird migration is a seasonal movement between breeding and wintering grounds.Opinions are widely divided on the reasons for this movement.According to biological data including:geographic distribution,reproductive phy...Bird migration is a seasonal movement between breeding and wintering grounds.Opinions are widely divided on the reasons for this movement.According to biological data including:geographic distribution,reproductive physiology,comparison of breeding and wintering habitats,geological data including Qinghai Tibetan Plateau uplift and Quatemary Period glaciation,it is inferred that bird migration is a survival mechanism,and that migration originated on the breeding grounds.展开更多
Parallel versions of prestack KirchhofT 3D integral migration algorithm, which is suitable forseismic data processing, are described in this paper. Firstly, the inherent parallel characteristics of seismicdata process...Parallel versions of prestack KirchhofT 3D integral migration algorithm, which is suitable forseismic data processing, are described in this paper. Firstly, the inherent parallel characteristics of seismicdata processing are analyzed. Then some principles in algorithm partition are discussed. Based on these analyses and the system architecture, communication mechanism, this algorithm is divided into four subtasksallocated to four nodes of 990 STAR-l. Then we describe in detail a module-partitioning method-theI / O processing and communication are separated from the computation process, the processes includingI / O processing and communication are allocated to transputer T805 and the other is allocated to processori860. These two processes are synchronized by shared memory and memory-lock mechanism, but the communication betWeen different nodes is implemented through links of transputer. Load balance among fourprocessor modules is performed dynamically. Finally, we discussed the speed--up of the parallel versions ofprestack KirchhofT 3D integral migration algorithm running on four nodes. Some further researches are also melltioned in this paper.展开更多
Nowadays,a certain amount of landfills in China were constructed without horizontal liner system.The research conducted focuses mainly on the contaminants from landfill leachate migrating in the aquifer of a fractured...Nowadays,a certain amount of landfills in China were constructed without horizontal liner system.The research conducted focuses mainly on the contaminants from landfill leachate migrating in the aquifer of a fractured granite area,and pollution predictions for groundwater were made by establishing numerical model with Visual Modflow combining field investigation like geological surveys,drilling,geophysical explorations,hydrogeological experiments,water quality analysis.The transportation of the chloride ion from landfill in the aquifer was simulated in the model with time frames of 2 555,3 650,5 475 and7 300 d.The model shows that from 2 555 d to 7 300 d starting from 2003,the chloride ion migrated from 900 m to 1 300 m,respectively,along the groundwater flow.The results indicate that as leachate plume migrated in the aquifer,the concentration of the pollutants can be up to 19.74 to 251.76 times that of background value.The research proves that the leachate poses a threat to the local water body and offers a reference towards groundwater pollution prevention for fractured granite landfill sites.展开更多
In this study,a novel energy-efficient single-phase induction motor with three stator windings is proposed as shown in Fig 1.The winding A constitutes the first branch.The series connection of the capacitor C and the ...In this study,a novel energy-efficient single-phase induction motor with three stator windings is proposed as shown in Fig 1.The winding A constitutes the first branch.The series connection of the capacitor C and the winding B constitute the second branch,and the series connection of the capacitor C2 and the winding C constitute the third one.The above three branches are connected in parallel as shown in Fig.2.展开更多
Cell-matrix interaction is the key for understanding the cell behaviors,especially the mechanosensitivity of cell adhesion,cell migration and differentiation,etc.Cells are constantly probing,pushing and pulling on the...Cell-matrix interaction is the key for understanding the cell behaviors,especially the mechanosensitivity of cell adhesion,cell migration and differentiation,etc.Cells are constantly probing,pushing and pulling on the surrounding extracellular matrix.These cell-generated forces drive cell migration and tissue morphogenesis,and maintain the intrinsic mechanical tone of tissues.Therefore,knowledge of the spatial and temporal nature of these forces are essential to understanding when and where mechanical events come to play in both physiological and pathological settings.However,because of the complexity of cell geometry and insufficient展开更多
文摘Objective:Ovarian cancer(OC)ranks among the leading causes of mortality among the female cancers worldwide.Numerous studies have explored the development and progression of OC at multiple genetic regulatory levels.However,relatively few studies have explored the impact of post-translational modifications(PTM)on OC progression,which is essential for uncovering new therapeutic targets.This study aimed to systematically identify the key PTM types involved in OCprogression,and to explore and evaluate their translational potential as therapeutic targets.Methods:First,we utilized multiple general PTM antibodies to compare gross PTM levels between normal ovarian and OC tissues from clinical females.After identifying lactylation as the PTM with the most significant differences,we selected representative samples for label-free mass spectrometry to identify specific lactylation sites.Next,we transfected A2780(OC)cells with either wild-type(WT)or mutant(K192A[Q])poly(ADP-ribose)polymerase 1(PARP1)conjugated to enhanced green fluorescent protein(EGFP)with a StrepⅡpeptide tag and assessed various cellular indexes related to cell proliferation(clonogenicity assay),migration(scratch wound healing assay),and reactive oxygen species levels.Results:Pan-lactylation was significantly upregulated in clinical OC samples,with PARP1 lactylation at K192 being one of the most common modifications.The growth and migration of A2780 cells were markedly suppressed by overexpressing PARP1-WT but not mutant PARP1.Overexpressing PARP1 significantly downregulated the phosphorylation of extracellular signal-regulated kinases 1/2(ERK1/2).Conclusion:This study uncovered a novel PTM of PARP1 in OC,lactylation,and demonstrated that lactylation at K192 is crucial in regulating OC cell growth and migration via the ERK1/2 pathway.Further investigations are required to elucidate the broader functional implications of PARP1 lactylation and its therapeutic potential.
基金funded by Zhijian Laboratory Open Fund,Rocket Force University of Engineering(Grant No.2023-ZJSYS-KF01-03).
文摘During the storage of composite propellants, the migration of plasticizers and other unbonded additives at the interfaces of liner adhesives has garnered significant attention in understanding liner failure mechanisms, aging processes, and safety performance. However, there is currently no non-destructive and quantitative detection method for migration of plasticizers in propellant liner. In this study, we developed a HTPB sensing liner by incorporating conductive fillers-namely carbon black(CB), carbon nanotubes(CNTs), and graphene nanoplatelets(GNP)-into the HTPB matrix. The synergistic interaction between CNTs and GNP facilitates the formation of a tunneling conductive network that imparts electrical conductivity to the HTPB liner. To elucidate the functional relationship between conductivity and nitroglycerin(NG) migration, we applied the HTPB sensing liner onto double base propellant surfaces and measured both the conductivity of the sensing layer and NG migration during a 71°C accelerated aging experiment. The results shows that when CNTs/GNP content reaches 3wt%, there is an exponential correlation between conductivity and NG migration with a fitting degree of 0.9652;the average response sensitivity of ΔR/R0 relative to NG migration is calculated as 41.69, with an average deviation of merely5.67% between NG migrations derived from conductivity fittings compared to those obtained via TGA testing results. Overall, this sensing liner exhibits excellent capabilities for detecting NG migration nondestructively and quantitatively while offering a novel approach for assessing interfacial component migrations as well as debonding defects in propellants-a promising avenue for future self-monitoring strategies regarding propellant integrity.
基金supported by the National Natural Science Foundation of China(62101099)the Chinese Postdoctoral Science Foundation(2021M690558,2022T150100,2018M633352,2019T120825)+3 种基金the Young Elite Scientist Sponsorship Program(YESS20200082)the Aeronautical Science Foundation of China(2022Z017080001)the Open Foundation of Science and Technology on Electronic Information Control Laboratorythe Natural Science Foundation of Sichuan Province(2023NSFSC1386)。
文摘The detection of hypersonic targets usually confronts range migration(RM)issue before coherent integration(CI).The traditional methods aiming at correcting RM to obtain CI mainly considers the narrow-band radar condition.However,with the increasing requirement of far-range detection,the time bandwidth product,which is corresponding to radar’s mean power,should be promoted in actual application.Thus,the echo signal generates the scale effect(SE)at large time bandwidth product situation,influencing the intra and inter pulse integration performance.To eliminate SE and correct RM,this paper proposes an effective algorithm,i.e.,scaled location rotation transform(ScLRT).The ScLRT can remove SE to obtain the matching pulse compression(PC)as well as correct RM to complete CI via the location rotation transform,being implemented by seeking the actual rotation angle.Compared to the traditional coherent detection algorithms,Sc LRT can address the SE problem to achieve better detection/estimation capabilities.At last,this paper gives several simulations to assess the viability of ScLRT.
基金Chinese Joint Seismological Science Foundation (100120) and State Key Basic Research Development and Programming Project of China (G199804070102).
文摘Lateral migration of fault activity in Weihe basin is a popular phenomenon and its characteristics are also typical. Taking the activity migrations of Wangshun Mountain piedmont fault toward Lishan piedmont fault and Weinan platform front fault, Dabaopi-Niujiaojian fault toward Shenyusi-Xiaojiazhai fault, among a serial of NE-trending faults from Baoji city to Jingyang County as examples, their migration time and process are analyzed and discussed in the present paper. It is useful for further understanding the structure development and physiognomy evolution history of Weihe basin.
文摘Based on arbitrarily wide-angle wave equations,a reverse-time propagation scheme is developed by substituting the partial derivatives of depth and time with central differences. The partial derivative of horizontal direction is replaced with high order difference. The imaging condition is computed by solving the eikonal equations. On the basis of above techniques,a prestack reverse-time depth migration algorithm is developed. The processing exam-ples of synthetic data show that the method can remove unwanted internal reflections and decrease the migration noise. The method also has the advantage of fidelity and is applicable of dip angle reflector imaging.
基金The project supported by National Natural Science Foundation of China(81502123,81330081,81202596)Natural Science Foundation of Anhui Province(1308085QH130)+3 种基金Anhui Province Natural Science Foundation in University(KJ2014A119)Grants for Scientific Research of BSKY from Anhui Medical University(XJ201212)Specialized Research Fund for the Doctoral Program of Higher Education(20113420120006,20123420110003)Program for Tackling Key Problems in Science and Technology by Anhui Province(1301042098)
文摘OBJECTIVE To investigate the role and mechanism of G protein-coupled receptor kinase 2(GRK2)involving in hepatocel ular carcinoma(HCC)progression.METHODS Cel Counting Kit 8 and tumor colony formation assay were designed to detect HCC cell proliferation,wound healing assay was to detect HCC migration.The correlation between GRK2 and early growth response-1(EGR1)were detected by RT-PCR and real-time PCR assays.Co-immunoprecipitation and Western blot assay were adopted to detect the relationship between GRK2and insulin-like growth factor 1 receptor(IGF-1R)signaling pathway.RESULTS In this study we find that GRK2plays an inhibition role in IGF1-induced HCC cell proliferation and migration.Overexpression of GRK2 causes a decrease in EGR1 expression,while knockdown of GRK2 leads to the dramatically increase in EGR1 expression in the treatment of IGF1.Through co-immunoprecipitation and Western blot assay,we confirm that GRK2can interact with IGF-1R and inhibiting IGF1-induced activation of IGF1R signaling pathway.Silencing EGR1attenuates GRK2 overexpression-caused inhibition of cell proliferation,tumor colony number and migrationactivity,while overexpressing of EGR1 restores the antiproliferative and migratory effect by GRK2 overexpression in HCCLM3 cells.CONCLUSION Taken together,these results suggest that GRK2 may inhibit IGF1-induced HCC cell growth and migration through down-regulation of EGR1 and indicate that enforced GRK2 may offer a potential therapeutic approach against HCC.
文摘The interactions on gold active and migratory quantities and rates between tuffaceous slate and solu tions with different compositions were experimentally studied at 200 ℃, 20 MPa, in a high pressure apparatus. After reaction, tuffaceous slate became light colored and soft, and its mass density reduced. The amount of gold extracted from tuffaceous slate ranges widely, from 0 027 to 0 234 μg/g. Chlorine solution may activate appreciable amount of gold, and the gold migratory rate is high enough, from 50 70% to 92 30%, which reveals that sulphur and chlorine work together in solutions to accelerate gold activation and migration, and to realize gold mineralization in favorable places.
基金Sponsored by Doctoral Foundation of Ministry of Education of China (20093219120006)
文摘The wind effects on steady-state scan characteristics and hit probability of terminal-sensitive projectile were discussed in this paper. Considering wind as the constitutions of the average wind and the impulsive wind, a simplified wind field model was established for the ballistic calculation of the steady-state scan phase; under the windy condition, the effects of the range wind and the beam wind on the steady-state scan characteristics of the terminal-sensitive projectile were analyzed in detail and its hit probabilities for a certain armored target were calculated. The calculated results show that, when the wind speed exceeds a certain value, the hit probabilities of terminal-sensitive projectile drop rapidly; the wind effects must be considered in the application of the terminal-sensitive projectiles. This paper provides some theoretical references for the fire wind speed correction and the global structure optimization of the terminal-sensitive projectile.
基金Project(50574104) supported by the National Natural Science Foundation of China
文摘Three observation methods were used to investigate the existing form and the behavior of rare earth during the sintering process of high activity mischmetal （RE, with lanthanum and cerium） doped WC-8%Co-0.048%RE（mass fraction） alloy with low carbon-containing level by scanning electron microscopy （SEM） and energy dispersive X-ray spectroscopy （EDXS）, considering the fact that the addition amount of rare earth in the alloy is very minute. The directional migration process and mechanism of cerium were discussed. First, the sinter skin （surface） is observed. oxide on the sinter skin, and lanthanum in these cerium observed, and lanthanum containing phase/micro-zone in It is shown that there exists a dispersedly distributed cerium containing enrichment positions is very minute. Secondly, the polished section is the alloy is identified. Finally, based on the fact that the fracture of cemented carbide is resulted from the heterogeneous phase or other defects within the microstructure, the fracture surface is observed and cerium containing phase/micro-zone in the fracture source approximately 260 μm from the surface is identified. These combined observations reveal adequately the fact that lanthanum and cerium get separated and cerium predominantly migrates towards the surface during the sintering process.
基金financially supported by the National Natural Science Foundation of China ( 11532009, 11602191,21775117)the General Financial Grant from the China Postdoctoral Science Foundation ( 2016M592773)the High Level Returned Overseas Students Foundation ( [2018]642)
文摘The migration mode transition of cancer cell enhances its invasive capability and the drug resistance,where physical confinement of cell microenvironment has been revealed to induce the mesenchymal-amoeboid transition(MAT).However,most existing studies are performed in PDMS microchannels,of which the stiffness is much higher than that of most mammalian tissues.Therefore,the amoeboid migration transition observed in these studies is actually induced by the synergistic effect of matrix stiffness and confinement.Since the stiffness of cell microenvironment has been reported to influence the cell migration in 2D substrate,the decoupling of stiffness and confinement effects is thus in need for elucidating the underlying mechanism of MAT.However,it is technically challenging to construct microchannels with physiologically relevant stiffness and channel size,where existing microchannel platforms with physiological relevance stiffness are all with>10μm channel width.Such size is too wide to mimic the physical confinement that migrating cancer cells confront in vivo,and also larger than the width of PDMS channel,in which the MAT of cancer cell was observed.Therefore,an in vitro cell migration platform,which could mimic both stiffness and confinement of the native physical microenvironment during cancer metastasis,could profoundly contribute to researches on cancer cell migration and cellular mechanotransduction.In this paper,we overcome the limitations of engineering soft materials in microscale by combining the collagen-alginate hydrogel with photolithography.This enables us to improve the accuracy of molded microchannel,and thus successfully construct a 3D microchannel platform,which matches the stiffness and width ranges of native environmental confinement that migrating cancer cells confront in vivo.The stiffness(0.3~20 kPa),confinement(channel width:3.5~14μm)and the adhesion ligand density of the microchannel can be tuned independently.Interestingly,using this platform,we observed that the migration speed of cancer cell is influenced by the synergistic effect of channel stiffness and width,and the increasing stiffness reverses the effect of channel width on the migration speed of cancer cells.In addition,MAT has a strong correlation with the channel stiffness.These findings make us reconsider the widely accepted hypothesis:physical confinement can induce MAT.Actually,this transition can only occur in stiff confined microenvironment not in soft one.For soft microchannels,the compliance of the channel walls could cause little cell/nucleus deformation,and the MAT could not be induced.To further investigate the mechanism of MAT,we developed a computational model to simulate the effect of nucleus deformation on MAT.With the model,we found that deforming the cell nuclear by decreasing the nucleus stiffness will reduce the cellmigration speed.This implies that nuclear stiffness plays an important role in the regulation of cancer migration speed and thus MAT in microchannels.The effect of channel stiffness on MAT and migration speed as observed in our experiment could partially explain previous findings reported in the literature,where the increasing matrix stiffness of tumor microenvironment promotes cancer metastasis.Our observations thus highlight the critical role of cell nuclear deformation not only in MAT,but also in regulating cellular mechanotransduction and cell-ECM interactions.This developed platform is capable of mimicking the native physical microenvironment during metastasis,providing a powerful tool for high-throughput screening applications and investigating the interaction between cancer migration and biophysical microenvironment.
基金the Heilongjiang Natural Science Fund Project (C2017035)。
文摘The purpose of this study was to explore the effects of recombinant human intestinal alkaline phosphatase(recIAP) on human neutrophils in vitro, and the migration, phagocytosis, apoptosis in presence and absence of LPS. In this study, freshly extracted human neutrophils were used to establish an inflammatory cell model, and the control group, recIAP group, LPS group and recIAP +LPS group were set up to stimulate the model. The migration of neutrophils was detected by agarose gel drop method. Fluorescent particles and fluorescent probes were added to different treatment groups, and the phagocytic rate of neutrophils and the release of reactive oxygen species(ROS) from neutrophils were detected by flow cytometry. The apoptosis rate of neutrophils was detected by flow cytometry according to Annexin V-FITC apoptosis detection kit. The results showed that regardless of the presence or absence of LPS, recIAP could inhibit the migration of neutrophils, phagocytosis and the release of ROS. In addition, recIAP could weaken the inhibitory effect of LPS on neutrophils apoptosis.
基金Project(41272122)supported by the National Natural Science Foundation of China
文摘The oblique transfer zone in the Fushan Sag, a syndepositional dome sandwiched between the Bailian and Huangtong sub-sags, has been the most important exploration target. The major oil observation occurs in the E_2l_1^(L+M) and the E_2l_3~U. 46 oil and rock samples reveal that the oil in the transfer zone is mostly contributed by the Bailian sub-sag, though the source rock conditions, hydrocarbon generation and expulsion histories of the Bailian and Huangtong sub-sags are similar. The E_2l_3~U oil, characterized by high maturity, Pr/Ph ratio and oleanane/C_(30)-hopane ratio, shows a close genetic affinity with the E_2l_3~b source rocks, while the E_2l_1^(L+M) oil, characterized by lower maturity, Pr/Ph ratio and oleanane/C_(30)-hopane ratio, is suggested to be derived from the E_2l_(1+2)~b source rocks. The homogenization temperatures of aqueous fluid inclusions, taking the burial history of the reservoirs into account, reflect that the oil charge mainly occurred from mid-Miocene to Pliocene in the oblique transfer zone. The oil transporting passages include connected sand bodies, unconformities and faults in the Fushan Sag. Of these, the faults are the most complicated and significant. The faults differ sharply in the west area, the east area and the oblique transfer zone, resulting in different influence on the oil migration and accumulation. During the main hydrocarbon charge stage, the faults in the west area are characterized by bad vertical sealing and spatially dense distribution. As a result, the oil generated by the Huangtong source rocks is mostly lost along the faults during the vertical migration in the west area. This can be the mechanism proposed to explain the little contribution of the Huangtong source rocks to the oil in the oblique transfer zone. Eventually, an oil migration and accumulation model is built in the oblique transfer zone, which may provide theoretical and practical guides for the oil exploration.
文摘OBJECTIVE This study was aimed to evaluate the effect of mangosteen peel extract(MPE)on migration of human umbilical vein endothelial cells(HUVECs)treated with LPS.METHODS Cultured HUVECs were divided into five groups:normal(untreated)group,positive control group treated with LPS at dose of 20ng·mL-1 for 24 h,and groups pre-treated with LPS at dose of 20ng·mL-1 for 24 h followed by incubation with MPE at doses of 1,2,and 4-1μg·mL,respectively.HUVECs migration was evaluated using wound-healing migration assay after 24 h of treatment.RESULTS LPS significantly(P<0.05)impaired HUVECs migration compared to the normal.MPE at dose of 1μg·mL-1 increased migration of LPS-treated HUVECs significantly(P<0.05)compared to the positive control and near to the normal level.CONCLUSION The mangosteen peel extract is able to dose-dependently preserve the endothelial cells function.
文摘A coupled heat and formaldehyde migration model based on the non-equilibrium thermodynamic theory and molecule movement theory was developed.The effect of temperature on the transport coefficients was simulated,and the simulation results were validated with experimental data from the literatures.The calculation shows that air exchange rate larger than 2 h-1 should be prevented,if the purpose is only for formaldehyde emissions control.The effects of temperature on formaldehyde migration are obvious.
文摘OBJECTIVE Prostate cancer is the most commonly diagnosed malignancy and the sixth leading cause of cancer death in men.Aberrant upregulation of enhancer of Zeste homolog2(EZH2)occur frequently in human cancers,including prostate adenocarcinoma,it′s a bona fide oncogene,yet clinical benefits of EZH2 inhibitor(EZH2i)remain unsatis⁃factory and limited to certain hematological malignancies.AD20 is a natural product which is extracted from creosote bush.Our previous study has demonstrated that AD20 could inhibit the growth and metastasis in prostate cancer by inhibiting NRP1,however,the mechanisms still need to be clarified.METHODS PC3 cell were cultured in DMEM/F12 supplemented with 10%fetal bovine serum.The proliferation and migration were measured by MTS,wound healing assay and tran⁃swell assay.The expression of the protein was observed by Western blot.The mRNA levels were detected by real-time PCR.LC-MS/MS-based proteomic assay,CoIP-MS and network pharmacology are used to construct the PPI network and identify related proteins.RESULTS AD20(10-20μmol·L^-1)can regulate EZH2 expression from transcription,trans⁃lation,degradation levels.AD20 downregulates EZH2-H3K27me3 signaling dramatically and activates many down⁃stream tumor suppressor genes,such as CDH1,GATA6,CDKN2A.In vitro,AD20(10-40μmol·L^-1)suppresses PC3 cell proliferation and migration in a dose-dependent manner.And interestingly,the combination with AD20 significantly improved the efficacy of EZH2 inhibitors(GSK126 and EPZ-6438)in PC3 cell line.CONCLUSION AD20 can suppress PC3 cell proliferation by targeting EZH2-H3K27me3-p16INK4a signaling and inhibit PC3 cell migration by inhibiting NRP1.
文摘Bird migration is a seasonal movement between breeding and wintering grounds.Opinions are widely divided on the reasons for this movement.According to biological data including:geographic distribution,reproductive physiology,comparison of breeding and wintering habitats,geological data including Qinghai Tibetan Plateau uplift and Quatemary Period glaciation,it is inferred that bird migration is a survival mechanism,and that migration originated on the breeding grounds.
文摘Parallel versions of prestack KirchhofT 3D integral migration algorithm, which is suitable forseismic data processing, are described in this paper. Firstly, the inherent parallel characteristics of seismicdata processing are analyzed. Then some principles in algorithm partition are discussed. Based on these analyses and the system architecture, communication mechanism, this algorithm is divided into four subtasksallocated to four nodes of 990 STAR-l. Then we describe in detail a module-partitioning method-theI / O processing and communication are separated from the computation process, the processes includingI / O processing and communication are allocated to transputer T805 and the other is allocated to processori860. These two processes are synchronized by shared memory and memory-lock mechanism, but the communication betWeen different nodes is implemented through links of transputer. Load balance among fourprocessor modules is performed dynamically. Finally, we discussed the speed--up of the parallel versions ofprestack KirchhofT 3D integral migration algorithm running on four nodes. Some further researches are also melltioned in this paper.
基金Project(12JJ305)supported by the Education Department of Hunan Province,China
文摘Nowadays,a certain amount of landfills in China were constructed without horizontal liner system.The research conducted focuses mainly on the contaminants from landfill leachate migrating in the aquifer of a fractured granite area,and pollution predictions for groundwater were made by establishing numerical model with Visual Modflow combining field investigation like geological surveys,drilling,geophysical explorations,hydrogeological experiments,water quality analysis.The transportation of the chloride ion from landfill in the aquifer was simulated in the model with time frames of 2 555,3 650,5 475 and7 300 d.The model shows that from 2 555 d to 7 300 d starting from 2003,the chloride ion migrated from 900 m to 1 300 m,respectively,along the groundwater flow.The results indicate that as leachate plume migrated in the aquifer,the concentration of the pollutants can be up to 19.74 to 251.76 times that of background value.The research proves that the leachate poses a threat to the local water body and offers a reference towards groundwater pollution prevention for fractured granite landfill sites.
文摘In this study,a novel energy-efficient single-phase induction motor with three stator windings is proposed as shown in Fig 1.The winding A constitutes the first branch.The series connection of the capacitor C and the winding B constitute the second branch,and the series connection of the capacitor C2 and the winding C constitute the third one.The above three branches are connected in parallel as shown in Fig.2.
基金supported by the National Natural Science Foundation of China through grant nos.11221202 and 11025208
文摘Cell-matrix interaction is the key for understanding the cell behaviors,especially the mechanosensitivity of cell adhesion,cell migration and differentiation,etc.Cells are constantly probing,pushing and pulling on the surrounding extracellular matrix.These cell-generated forces drive cell migration and tissue morphogenesis,and maintain the intrinsic mechanical tone of tissues.Therefore,knowledge of the spatial and temporal nature of these forces are essential to understanding when and where mechanical events come to play in both physiological and pathological settings.However,because of the complexity of cell geometry and insufficient
