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旅游小城镇规划中的Long Stay要素分析 被引量:4
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作者 王蕾 张阳生 刘晴 《安徽农业科学》 CAS 北大核心 2009年第31期15526-15527,共2页
根据小城镇具有发展Long Stay的条件,在进行小城镇规划建设中考虑Long Stay要素的体现,并对旅游小城镇的效益进行了分析。结果表明:小城镇具有发展Long Stay的条件,在城镇的总体规划中体现出其作用,并能对城镇产生经济效益与社会效益。
关键词 小城镇建设 LONG stay 效益
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Calculation model and mechanism analysis for rain-wind-induced vibration of stay cable 被引量:1
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作者 谢桂华 刘荣桂 +1 位作者 蔡东升 陈蓓 《Journal of Central South University》 SCIE EI CAS 2014年第3期1107-1114,共8页
Rain-wind-induced vibration of cable was studied based on previous research achievements. According to the quasi-steady assumption, the governing equation of vertical motion of the cable was derived and the criterion ... Rain-wind-induced vibration of cable was studied based on previous research achievements. According to the quasi-steady assumption, the governing equation of vertical motion of the cable was derived and the criterion for unstable motion and occurrence mechanism was studied. A comparison was performed between the oscillation responses of the stay cable obtained from calculated model and previous results. The results indicate that the analysis model can reflect the main characteristics of wind-rain-induced vibrationt of the cable which is amplitude- and velocity-restricted, and it is probably related with the periodic vortex shedding of wake flow. It is essential for the occurrence of rain-wind-induced or wind-induced vibration of cable that the derivative of lift coefficient with respect to transient angle of attack is less than zero. When rain-wind-induced vibration occurs, the aerodynamic force has a dual function for the vibration, and the maximum amplitude of stayed-cable is determined by the relative value of aerodynamic exciting force and aerodynamic damping force. 展开更多
关键词 rain-wind-induced vibration stay cable governing equation STABILITY vortex shedding
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难选铅锑锌分离新工艺研究 被引量:10
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作者 魏宗武 叶雪均 +1 位作者 陈建华 刘智林 《中国矿业》 北大核心 2006年第9期88-90,共3页
针对某选矿厂铅锑锌砂矿粒度粗,受到药剂污染严重,而泥矿未受药剂污染但粒度细的特点;根据砂矿与泥矿的不同浮选特性,提出了泥、砂矿铅锑分选,锌粗精矿合并再磨分选,并辅以抑硫新药剂Y1的浮选新工艺,获得了铅回收率81.52%,锌回收率82.59。
关键词 台浮硫化矿 抑硫新药剂 砂矿 泥矿 粗精矿再磨
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水稻SGR酵母双杂交诱饵载体的构建及鉴定
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作者 兰兆吉 吴国江 《安徽农业科学》 CAS 北大核心 2008年第17期7150-7151,7303,共3页
[目的]揭示SGR基因的作用机理。[方法]构建水稻SGR基因在酵母双杂交体系中的诱饵载体,并对其表达进行鉴定,将目的基因SGR与诱饵质粒载体pGBKT7通过双酶切定向重组构建诱饵质粒pGBKT7-SGR,将pGBKT7-SGR转入酵母菌株Y187中,利用Western印... [目的]揭示SGR基因的作用机理。[方法]构建水稻SGR基因在酵母双杂交体系中的诱饵载体,并对其表达进行鉴定,将目的基因SGR与诱饵质粒载体pGBKT7通过双酶切定向重组构建诱饵质粒pGBKT7-SGR,将pGBKT7-SGR转入酵母菌株Y187中,利用Western印迹法检测其在酵母中的表达,通过缺陷性培养基培养进行自激活检测。[结果]将鉴定正确的重组质粒测序结果与SGR基因比较,序列完全一致,阅读框分析正确。载体pGBKT7-SGR在酵母菌株Y187中可以正确表达出融合蛋白。重组诱饵pGBKT7-SGR质粒对酵母无毒性,其表达产物不能激活酵母菌株Y187的营养缺陷型报告基因。[结论]该重组诱饵质粒可用于酵母双杂交体系,该研究为从cDNA文库筛选水稻诱饵蛋白SGR的互作蛋白奠定了基础。 展开更多
关键词 stay green 酵母双杂交系统 westem印迹 自激活检测
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