Nanopipette based scanning probe technique is a versatile tool in non-contact imaging in biology.In addition to the topographic imaging,its capability of localized delivery of bio-active molecules is emerging.In this ...Nanopipette based scanning probe technique is a versatile tool in non-contact imaging in biology.In addition to the topographic imaging,its capability of localized delivery of bio-active molecules is emerging.In this mini review,we introduce the applications of nanopipette in single-cell researches with a focus on localized delivery.The working principles of three delivery modes including resistive pulse,pressure-driven flow,and electroosmotic flow-driven delivery are summarized and compared.Their applications in single-cell researches are reviewed.The current technical challenges in scanning ion conductance microscopy-based delivery,and their growing influence in medicine and pharmacologic researches are also discussed.展开更多
Single pixel imaging is a novel imaging technique,and it becomes a focus of research in recent years due to its advantages such as high lateral resolution and high robustness to noise.Imaging speed is one of the criti...Single pixel imaging is a novel imaging technique,and it becomes a focus of research in recent years due to its advantages such as high lateral resolution and high robustness to noise.Imaging speed is one of the critical shortcomings,which limits the further development and applications of this technique.In this paper,we focus on the issues of imaging efficiency of a single pixel imaging system.We propose semi-continuous wavelet transform(SCWT)protocol and introduce the protocol into the single pixel imaging system.The proposed protocol is something between continuous wavelet transform and discrete wavelet transform,which allows the usage of those smooth(usually non-orthogonal,and they have advantages in representing smooth signals compressively,which can improve the imaging speed of single pixel imaging)wavelets and with limited numbers of measurements.The proposed imaging scheme is studied,and verified by simulations and experiments.Furthermore,a comparison between our proposed scheme and existing imaging schemes are given.According to the results,the proposed SCWT scheme is proved to be effective in reconstructing a image compressively.展开更多
In recent years,deep learning has been introduced into the field of Single-pixel imaging(SPI),garnering significant attention.However,conventional networks still exhibit limitations in preserving image details.To addr...In recent years,deep learning has been introduced into the field of Single-pixel imaging(SPI),garnering significant attention.However,conventional networks still exhibit limitations in preserving image details.To address this issue,we integrate Large Kernel Convolution(LKconv)into the U-Net framework,proposing an enhanced network structure named U-LKconv network,which significantly enhances the capability to recover image details even under low sampling conditions.展开更多
According to the operational conditions of an aviation module reticule,a measurement mode is proposed,which is based on an industrial photogrammetry system,with matching by a measuring pen.Meanwhile,the factors affect...According to the operational conditions of an aviation module reticule,a measurement mode is proposed,which is based on an industrial photogrammetry system,with matching by a measuring pen.Meanwhile,the factors affecting the accuracy of the measurement have been analyzed and verified by examples.The analysis is described as follows:①Along the optical axis of the camera,the error is larger than the ones in other directions using the“single camera+measuring pen”mode;②By avoiding the error along the optical axis of the camera,the accuracy of the“single camera+measuring pen”mode is better than 0.1 mm when the measuring pen is moving parallel to the optical axis.展开更多
基金support from the National Institute of General Medical Sciences(1R35G M147172-01).
文摘Nanopipette based scanning probe technique is a versatile tool in non-contact imaging in biology.In addition to the topographic imaging,its capability of localized delivery of bio-active molecules is emerging.In this mini review,we introduce the applications of nanopipette in single-cell researches with a focus on localized delivery.The working principles of three delivery modes including resistive pulse,pressure-driven flow,and electroosmotic flow-driven delivery are summarized and compared.Their applications in single-cell researches are reviewed.The current technical challenges in scanning ion conductance microscopy-based delivery,and their growing influence in medicine and pharmacologic researches are also discussed.
基金the Natural Science Foundation of Jilin Province,China(Grand No.YDZJ202101ZYTS030)。
文摘Single pixel imaging is a novel imaging technique,and it becomes a focus of research in recent years due to its advantages such as high lateral resolution and high robustness to noise.Imaging speed is one of the critical shortcomings,which limits the further development and applications of this technique.In this paper,we focus on the issues of imaging efficiency of a single pixel imaging system.We propose semi-continuous wavelet transform(SCWT)protocol and introduce the protocol into the single pixel imaging system.The proposed protocol is something between continuous wavelet transform and discrete wavelet transform,which allows the usage of those smooth(usually non-orthogonal,and they have advantages in representing smooth signals compressively,which can improve the imaging speed of single pixel imaging)wavelets and with limited numbers of measurements.The proposed imaging scheme is studied,and verified by simulations and experiments.Furthermore,a comparison between our proposed scheme and existing imaging schemes are given.According to the results,the proposed SCWT scheme is proved to be effective in reconstructing a image compressively.
文摘In recent years,deep learning has been introduced into the field of Single-pixel imaging(SPI),garnering significant attention.However,conventional networks still exhibit limitations in preserving image details.To address this issue,we integrate Large Kernel Convolution(LKconv)into the U-Net framework,proposing an enhanced network structure named U-LKconv network,which significantly enhances the capability to recover image details even under low sampling conditions.
文摘According to the operational conditions of an aviation module reticule,a measurement mode is proposed,which is based on an industrial photogrammetry system,with matching by a measuring pen.Meanwhile,the factors affecting the accuracy of the measurement have been analyzed and verified by examples.The analysis is described as follows:①Along the optical axis of the camera,the error is larger than the ones in other directions using the“single camera+measuring pen”mode;②By avoiding the error along the optical axis of the camera,the accuracy of the“single camera+measuring pen”mode is better than 0.1 mm when the measuring pen is moving parallel to the optical axis.
