Objective:To evaluate the effect of cooling of whole blood samples without an anticoagulant and glucose preservative on glucose and other commonly measured clinical analytes by serial measurements over a three hour pe...Objective:To evaluate the effect of cooling of whole blood samples without an anticoagulant and glucose preservative on glucose and other commonly measured clinical analytes by serial measurements over a three hour period. Methods: Thirty healthy adult volunteers about 20-50 years old,from Kasturba Medical College,Manipal (2008) were the participants. After obtaining informed consent from each participant,blood was collected (2 mL each) in three vacutainers-one without the use of an anticoagulant and glucose preservative,one with sodium fluoride-potassium oxalate and the other with disodium ethylene diamine tetraacetic (EDTA). Automatic analyzer Hitachi was used to measure glucose and other common clinical laboratory analytes,every hour for three hours. Results: The refrigerated samples without an anticoagulant and preservative,showed slightly lower values of glucose as compared to fluorinated samples but difference between these groups was not statistically significant (P value> 0.05).Also cooling had no effect on other analytes except potassium.Conclusion: Fluoride preservative as a regular practice for glucose samples can be avoided if the samples can be processed quickly as fluoriated samples also show a marginal fall in glucose values.展开更多
A new non-invasive blood glucose measuring apparatus (NBGMA) made up of MSP430F149 SCM (single chip micyoco) was developed,which can measure blood glucose level (BGL) frequently,conveniently and painlessly. The hardwa...A new non-invasive blood glucose measuring apparatus (NBGMA) made up of MSP430F149 SCM (single chip micyoco) was developed,which can measure blood glucose level (BGL) frequently,conveniently and painlessly. The hardware and software of this apparatus were designed,and detecting algorithms based on conservation of energy method (COEM) were presented. According to the law of conservation of energy that the energy derived by human body equals energy consumed by metabolism,and the relationship between convection,evaporation,radiation and the BGL was established. The sensor module was designed. 20 healthy volunteers were involved in the clinical experiment. The BGL measured by an automatic biochemical analyzer (ABA) was set as the reference. Regression analysis was performed to compare the conservation of energy method with the biochemical method,using the 20 data points with blood glucose concentrations ranging from 680 to 1 100 mg/L. Reproducibility was measured for healthy fasting volunteers. The results show that the means of BGL detected by NBGMA and ANA are very close to each other,and the difference of standard deviation (SD) is 24.7 mg/L. The correlative coefficient is 0.807. The coefficient of variation (CV) is 4% at 921.6 mg/L. The resultant regression is evaluated by the Clarke error grid analysis (EGA) and all data points are included in the clinically acceptable regions (region A:100%,region B:0%). Accordingly,it is feasible to measure BGL with COEM.展开更多
文摘Objective:To evaluate the effect of cooling of whole blood samples without an anticoagulant and glucose preservative on glucose and other commonly measured clinical analytes by serial measurements over a three hour period. Methods: Thirty healthy adult volunteers about 20-50 years old,from Kasturba Medical College,Manipal (2008) were the participants. After obtaining informed consent from each participant,blood was collected (2 mL each) in three vacutainers-one without the use of an anticoagulant and glucose preservative,one with sodium fluoride-potassium oxalate and the other with disodium ethylene diamine tetraacetic (EDTA). Automatic analyzer Hitachi was used to measure glucose and other common clinical laboratory analytes,every hour for three hours. Results: The refrigerated samples without an anticoagulant and preservative,showed slightly lower values of glucose as compared to fluorinated samples but difference between these groups was not statistically significant (P value> 0.05).Also cooling had no effect on other analytes except potassium.Conclusion: Fluoride preservative as a regular practice for glucose samples can be avoided if the samples can be processed quickly as fluoriated samples also show a marginal fall in glucose values.
基金Project(07JJ6133) supported by the Natural Science Foundation of Hunan Province, China
文摘A new non-invasive blood glucose measuring apparatus (NBGMA) made up of MSP430F149 SCM (single chip micyoco) was developed,which can measure blood glucose level (BGL) frequently,conveniently and painlessly. The hardware and software of this apparatus were designed,and detecting algorithms based on conservation of energy method (COEM) were presented. According to the law of conservation of energy that the energy derived by human body equals energy consumed by metabolism,and the relationship between convection,evaporation,radiation and the BGL was established. The sensor module was designed. 20 healthy volunteers were involved in the clinical experiment. The BGL measured by an automatic biochemical analyzer (ABA) was set as the reference. Regression analysis was performed to compare the conservation of energy method with the biochemical method,using the 20 data points with blood glucose concentrations ranging from 680 to 1 100 mg/L. Reproducibility was measured for healthy fasting volunteers. The results show that the means of BGL detected by NBGMA and ANA are very close to each other,and the difference of standard deviation (SD) is 24.7 mg/L. The correlative coefficient is 0.807. The coefficient of variation (CV) is 4% at 921.6 mg/L. The resultant regression is evaluated by the Clarke error grid analysis (EGA) and all data points are included in the clinically acceptable regions (region A:100%,region B:0%). Accordingly,it is feasible to measure BGL with COEM.
