The condition of weightes non-dictatorship is extended and a comprehensive evaluae method emboding self-determinate which is combined with competitive view optimization principles is built. The basic process includes ...The condition of weightes non-dictatorship is extended and a comprehensive evaluae method emboding self-determinate which is combined with competitive view optimization principles is built. The basic process includes simulating the model of economic man＇s self-benefit bahaviors, taking the place of experts to evaluate, bringing in the model of minimizing the sum of included angles to integrate the information of multiple objects and put the objects in order finally. The method has the advangtages of less dependendence on the subjective information, plenty of information, fair process and simple caculating. Finally, an application example is given to illustrate the effectiveness of the proposed method.展开更多
通过热水浸提法从荞麦中提取水溶性多糖,并用离子交换柱和凝胶过滤柱对荞麦多糖进行分离纯化、测定其分子量。结果表明,荞麦多糖粗品用Savag法脱蛋白后,采用DEAE-Sepharose Fast Flow和Sephacryl S-400HR柱层析纯化,得到荞麦多糖(FEP),...通过热水浸提法从荞麦中提取水溶性多糖,并用离子交换柱和凝胶过滤柱对荞麦多糖进行分离纯化、测定其分子量。结果表明,荞麦多糖粗品用Savag法脱蛋白后,采用DEAE-Sepharose Fast Flow和Sephacryl S-400HR柱层析纯化,得到荞麦多糖(FEP),经过凝胶柱色谱分析表明,FEP为均一多糖。用苯酚硫酸法测定多糖的含量,用AKTA快速液相蛋白层析仪的紫外检测器在280nm波长处检测蛋白质含量。最后根据用不同分子量标准葡聚糖做出的标准曲线,测定出FEP分子量(Mw)为1720442D。展开更多
基金supported by the National Natural Science Foundation of China(70801013)LNSTF for doc-tor(20081020).
文摘The condition of weightes non-dictatorship is extended and a comprehensive evaluae method emboding self-determinate which is combined with competitive view optimization principles is built. The basic process includes simulating the model of economic man＇s self-benefit bahaviors, taking the place of experts to evaluate, bringing in the model of minimizing the sum of included angles to integrate the information of multiple objects and put the objects in order finally. The method has the advangtages of less dependendence on the subjective information, plenty of information, fair process and simple caculating. Finally, an application example is given to illustrate the effectiveness of the proposed method.
文摘通过热水浸提法从荞麦中提取水溶性多糖,并用离子交换柱和凝胶过滤柱对荞麦多糖进行分离纯化、测定其分子量。结果表明,荞麦多糖粗品用Savag法脱蛋白后,采用DEAE-Sepharose Fast Flow和Sephacryl S-400HR柱层析纯化,得到荞麦多糖(FEP),经过凝胶柱色谱分析表明,FEP为均一多糖。用苯酚硫酸法测定多糖的含量,用AKTA快速液相蛋白层析仪的紫外检测器在280nm波长处检测蛋白质含量。最后根据用不同分子量标准葡聚糖做出的标准曲线,测定出FEP分子量(Mw)为1720442D。
