Background Ensuring that seeds germinate and emerge normally is a prerequisite for cotton production,esp.in areas with salinized soil.Priming with mepiquat chloride(MC)can promote seed germination and root growth unde...Background Ensuring that seeds germinate and emerge normally is a prerequisite for cotton production,esp.in areas with salinized soil.Priming with mepiquat chloride(MC)can promote seed germination and root growth under salt stress,but its mechanism has not been fully elucidated.In this study,physiological and biochemical experiments revealed that MC-priming promotes the tolerance of cotton seeds to salt stress by increasing the ability of antioxidant enzymes related to the ascorbate-glutathione(AsA-GSH)cycle to scavenge reactive oxygen species(ROS).Results Results revealed that treatment with inhibitors of abscisic acid(ABA)and γ-aminobutyric acid(GABA)biosynthesis reduced the positive effects of MC-priming.Similarly,MC-priming increased the contents of ABA and GABA under salt stress by stimulating the expression levels of GhNCED2 and GhGAD4 and the activity of calmodulin-binding(CML)glutamate decarboxylase(GAD).Further analysis showed that an inhibitor of ABA synthesis reduced the positive impacts of MC-priming on the content of GABA under salt stress,but the content of ABA was not affected by the GABA synthesis inhibitor.Furthermore,a multi-omics analysis revealed that MC-priming increased the abundance and phosphorylation levels of the proteins related to ABA signaling,CML,and Ca^(2+)channels/transporters in the MC-primed treatments,which resulted in increased oscillations in Ca^(2+)in the MC-primed cotton seeds under salt stress.Conclusion In summary,these results demonstrate that MC-mediated ABA signaling operates upstream of the GABA synthesis generated by GAD by activating the oscillations of Ca^(2+)and then enhancing activity of the AsA-GSH cycle,which ensures that cotton seeds are tolerant to salt stress.展开更多
This paper presents a modified Root-MUSIC algorithm by which the signal DOA estimation performance can be improved when the snapshot number is limited. The operation principlesof this algorithm are described in detail...This paper presents a modified Root-MUSIC algorithm by which the signal DOA estimation performance can be improved when the snapshot number is limited. The operation principlesof this algorithm are described in detail. It is also pointed out theoretically that this is equivalentto have increased the snapshot number and can make the DOA estimation better. Finally, somesimulating results to verify the theoretical analyses are presented.展开更多
A polynomial-rooting based fourth-order cumulant algorithm is presented for direction-of-arrival（DOA） estimation of second-order fully noncircular source signals, using a uniform linear array（ULA）. This algorithm ...A polynomial-rooting based fourth-order cumulant algorithm is presented for direction-of-arrival（DOA） estimation of second-order fully noncircular source signals, using a uniform linear array（ULA）. This algorithm inherits all merits of its spectralsearching counterpart except for the applicability to arbitrary array geometry, while reducing considerably the computation cost.Simulation results show that the proposed algorithm outperforms the previously developed closed-form second-order noncircular ESPRIT method, in terms of processing capacity and DOA estimation accuracy, especially in the presence of spatially colored noise.展开更多
基金supported by the National Natural Science Foundation of China(32001481)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences+3 种基金the China Agriculture Research System,the National Modern Agricultural Industry Technology System of China(CARS-18–05)the Provincial Key R&D and Promotion Special Projects in Henan(232102110178)the Program for Key Areas of Science and Technology of Xinjiang Production and Construction Corps Third Division and Tumsuk City(KY2021GG08)the Central Public-interest Scientific Institution Basal Research Fund(1610162023019)。
文摘Background Ensuring that seeds germinate and emerge normally is a prerequisite for cotton production,esp.in areas with salinized soil.Priming with mepiquat chloride(MC)can promote seed germination and root growth under salt stress,but its mechanism has not been fully elucidated.In this study,physiological and biochemical experiments revealed that MC-priming promotes the tolerance of cotton seeds to salt stress by increasing the ability of antioxidant enzymes related to the ascorbate-glutathione(AsA-GSH)cycle to scavenge reactive oxygen species(ROS).Results Results revealed that treatment with inhibitors of abscisic acid(ABA)and γ-aminobutyric acid(GABA)biosynthesis reduced the positive effects of MC-priming.Similarly,MC-priming increased the contents of ABA and GABA under salt stress by stimulating the expression levels of GhNCED2 and GhGAD4 and the activity of calmodulin-binding(CML)glutamate decarboxylase(GAD).Further analysis showed that an inhibitor of ABA synthesis reduced the positive impacts of MC-priming on the content of GABA under salt stress,but the content of ABA was not affected by the GABA synthesis inhibitor.Furthermore,a multi-omics analysis revealed that MC-priming increased the abundance and phosphorylation levels of the proteins related to ABA signaling,CML,and Ca^(2+)channels/transporters in the MC-primed treatments,which resulted in increased oscillations in Ca^(2+)in the MC-primed cotton seeds under salt stress.Conclusion In summary,these results demonstrate that MC-mediated ABA signaling operates upstream of the GABA synthesis generated by GAD by activating the oscillations of Ca^(2+)and then enhancing activity of the AsA-GSH cycle,which ensures that cotton seeds are tolerant to salt stress.
文摘This paper presents a modified Root-MUSIC algorithm by which the signal DOA estimation performance can be improved when the snapshot number is limited. The operation principlesof this algorithm are described in detail. It is also pointed out theoretically that this is equivalentto have increased the snapshot number and can make the DOA estimation better. Finally, somesimulating results to verify the theoretical analyses are presented.
基金supported by the National Natural Science Foundation of China(617020986170209961331019)
文摘A polynomial-rooting based fourth-order cumulant algorithm is presented for direction-of-arrival（DOA） estimation of second-order fully noncircular source signals, using a uniform linear array（ULA）. This algorithm inherits all merits of its spectralsearching counterpart except for the applicability to arbitrary array geometry, while reducing considerably the computation cost.Simulation results show that the proposed algorithm outperforms the previously developed closed-form second-order noncircular ESPRIT method, in terms of processing capacity and DOA estimation accuracy, especially in the presence of spatially colored noise.
