Blood cells are mainly(~99%)comprised of red blood cells.The most remarkable properties of are their high deformability,which allow they flow through microcapillaries of diameter even smaller than their size.The RBC’...Blood cells are mainly(~99%)comprised of red blood cells.The most remarkable properties of are their high deformability,which allow they flow through microcapillaries of diameter even smaller than their size.The RBC’s remarkable mechanical properties originate from the unique architecture of its membrane.To study the mechanism of RBC’s deformability,a commonly adopted approach is to localize the cytoskeleton protein by immunofluorescence,followed by exploring the changes of cytoskeleton protein during cell deformation.During this process,the fixed treatment of RBC using GA and PFA is of great importance.However,RBC’s deformability is reduced by the fixation process and skeletal protein of membrane is changed accordingly.The flow behavior of red RBCs through the microchannel also changed.Given the difficulty of observing RBC flow in vivo,in vitro simulation by virtue of microfluidic devices provides a feasible alternative.An important physiological phenomenon of the blood flow is the formation of cell free layer(CFL),with RBCs show a tendency to concentrate towards the central axis of the pipeline and move faster than the plasma layer.However,this phenomena is weaken if the stiffness of the membrane increase,which occurs in some disease,such as hereditary spherocytosis and hereditary elliptocytosis.To study the effects of GA and PFA fix treatment on RBC deformability,a microfluidic platform is employed to measuring the CFL and flow velocity of blood flow in this work.The PDMS micro flow channel used is 100 micrometers in width and 50 micrometers in deep.The RBC suspension is fed into the flow channel by the injection pump(NE-1000.USA),and the experiments are observed and recorded by the inverted microscope(IX70,Olympus,Japan)and high-speed camera(Memrecam GX-1,NAC,Japan)system.Three GA concentrations,i.e.,0.000 5,0.000 75,and 0.001 wt.%were used.Meanwhile,the effect of PFA at a concentration of 2wt.%work with GA was also investigated.Images of the flowing RBCs are processed mainly based on Memrecam GXLink.The results show that,the diameter of the RBC be treated is bigger and the shape of the RBC is became more flat after treated.Some of RBCs lost their biconcave structure.When the RBC suspension with 5%Hct flow in the microchannel,the CFL thickness decrease after being treated.And with concentrations of GA increase,the CFL thickness become thinner.The CFL thickness decrease significantly when GA and 2 wt.%PFA work combined.The velocity of RBCs decreases after treated with the GA or/and 2wt.%PFA.GA is known to relieve the dissolution of red blood cells during fluorescence labeling.On the other hand,the crosslinking of the aldehyde group(-cho)of GA with the amino group(-nh2)of RBC membrane protein will change the conformation of the membrane protein and its visco-elastic properties in turn.Then,the transparent fluidity orrheology characteristics of RBC is altered.Since GA and PFA are commonly used to immobilize red blood cells and keep the fluorescence constant,and PFA works similarly as GA,as a result,the variation of membrane protein conformation is intensified,and the membrane becomes stiffer.展开更多
目的:探讨平均红细胞体积(m ean corpuscu lar volum e,MCV)及红细胞体积分布宽度(red b lood cell volum e d istribution w idth,RDW)对地中海贫血(简称地贫,MD)和巨幼细胞性贫血(简称巨幼贫血,MA)诊断的临床价值。方法:利用血细胞自...目的:探讨平均红细胞体积(m ean corpuscu lar volum e,MCV)及红细胞体积分布宽度(red b lood cell volum e d istribution w idth,RDW)对地中海贫血(简称地贫,MD)和巨幼细胞性贫血(简称巨幼贫血,MA)诊断的临床价值。方法:利用血细胞自动分析仪测定不同病因的贫血患者的MCV和RDW值。结果:MD患者,MCV降低,RDW升高,且α-地贫与β-地贫无差异;MA患者,MCV和RDW升高;急性失血性贫血和再生障碍性贫血(ap lastic anem ia,AA)患者,MCV和RDW正常。结论:MCV和RDW红细胞参数,可作为贫血病因诊断及鉴别诊断的参考线索,对地贫和巨幼贫血的诊断和治疗有一定的指导意义。展开更多
目的:探讨大株红景天联用曲美他嗪对冠心病合并心衰患者疗效及红细胞分布宽度(RDW,Red Blood Cell Distribution Width)、生长分化因子15(GDF-15,Growth and Differentiation factor 15)水平的影响。方法:选择在本院接受治疗的冠心病合...目的:探讨大株红景天联用曲美他嗪对冠心病合并心衰患者疗效及红细胞分布宽度(RDW,Red Blood Cell Distribution Width)、生长分化因子15(GDF-15,Growth and Differentiation factor 15)水平的影响。方法:选择在本院接受治疗的冠心病合并心衰患者104例,随机分为对照组和观察组,每组52例。对照组入院后,根据病情病程给予患者常规的对症治疗:包括血管紧张素转换酶抑制剂或血管紧张素受体拮抗剂、利尿制剂、β受体阻滞剂等药物。观察组在对照组治疗措施的基础上加用大株红景天注射液联合曲美他嗪。疗程结束后,对两组治疗效果展开评估,对患者治疗前后心功能的各项指标进行检测,主要包括:左心室舒张末期内径(LEVDD)、左心室收缩末期内径(LVESD)、左心室射血分数(LVEF)指标;分析比较两组患者治疗前后RDW和GDF-15水平变化。结果:观察组总有效率明显高于对照组(χ~2=8.101,P=0.003);治疗后观察组的LVEF值相比于对照组明显增高,但是观察组LVESD,LVEDD显著低于对照组;治疗后,观察组GDF-15含量显著低于对照组,差异有统计学意义(P<0.05);观察组RDW低于对照组,差异无统计学意义(P>0.05)。结论:大株红景天联用曲美他嗪可有效治疗冠心病合并心衰患者,并能有效降低RDW和GDF-15水平,具有较好的临床应用前景。展开更多
目的:探讨红细胞分布宽度(red blood cell distribution width,RDW)、中性粒细胞淋巴细胞比值(neutrophil to lymphocyte ratio,NLR)联合终末期肝病模型(model for end-stage liver disease,MELD)评分评估失代偿期肝硬化患者预后的价值...目的:探讨红细胞分布宽度(red blood cell distribution width,RDW)、中性粒细胞淋巴细胞比值(neutrophil to lymphocyte ratio,NLR)联合终末期肝病模型(model for end-stage liver disease,MELD)评分评估失代偿期肝硬化患者预后的价值。方法:回顾性分析2013年1月-2016年12月本院确诊的肝硬化失代偿期患者181例,以死亡为终点事件,根据患者随访1年的疾病转归情况分为生存组(n=126)和死亡组(n=55),比较两组患者入院时RDW、NLR、MELD水平差异,运用多因素Cox风险比例模型预测患者1年死亡的独立影响因素,绘制受试者工作特征(ROC)曲线,比较ROC曲线下面积(AUC),应用Kaplan-Meier曲线分析不同RDW、NLR患者的1年生存率变化。最后通过二分类变量Logistic回归对RDW、NLR、MELD评分进行联合,评估多变量联合预测患者1年死亡的价值。结果:181例随访1年死亡55例,存活126例,死亡组入院时NLR、RDW、MELD评分均高于生存组(P<0.05);多因素Cox回归分析后显示,NLR、RDW及MELD评分是预测患者1年死亡的独立危险因素;RDW、NLR及MELD评分预测患者1年预后最佳临界值分别为14.65%、2.58、16.65,ROC曲线下面积(AUC)分别为0.652、0.764、0.862;MELD评分联合NLR的AUC为0.870,MELD评分联合RDW的AUC为0.876,MELD、RDW、NLR三者联合的AUC提高至0.884。结论:MELD、RDW、NLR三者联合对失代偿期肝硬化患者1年预后有较高的预测价值,优于单独MELD评分。展开更多
文摘Blood cells are mainly(~99%)comprised of red blood cells.The most remarkable properties of are their high deformability,which allow they flow through microcapillaries of diameter even smaller than their size.The RBC’s remarkable mechanical properties originate from the unique architecture of its membrane.To study the mechanism of RBC’s deformability,a commonly adopted approach is to localize the cytoskeleton protein by immunofluorescence,followed by exploring the changes of cytoskeleton protein during cell deformation.During this process,the fixed treatment of RBC using GA and PFA is of great importance.However,RBC’s deformability is reduced by the fixation process and skeletal protein of membrane is changed accordingly.The flow behavior of red RBCs through the microchannel also changed.Given the difficulty of observing RBC flow in vivo,in vitro simulation by virtue of microfluidic devices provides a feasible alternative.An important physiological phenomenon of the blood flow is the formation of cell free layer(CFL),with RBCs show a tendency to concentrate towards the central axis of the pipeline and move faster than the plasma layer.However,this phenomena is weaken if the stiffness of the membrane increase,which occurs in some disease,such as hereditary spherocytosis and hereditary elliptocytosis.To study the effects of GA and PFA fix treatment on RBC deformability,a microfluidic platform is employed to measuring the CFL and flow velocity of blood flow in this work.The PDMS micro flow channel used is 100 micrometers in width and 50 micrometers in deep.The RBC suspension is fed into the flow channel by the injection pump(NE-1000.USA),and the experiments are observed and recorded by the inverted microscope(IX70,Olympus,Japan)and high-speed camera(Memrecam GX-1,NAC,Japan)system.Three GA concentrations,i.e.,0.000 5,0.000 75,and 0.001 wt.%were used.Meanwhile,the effect of PFA at a concentration of 2wt.%work with GA was also investigated.Images of the flowing RBCs are processed mainly based on Memrecam GXLink.The results show that,the diameter of the RBC be treated is bigger and the shape of the RBC is became more flat after treated.Some of RBCs lost their biconcave structure.When the RBC suspension with 5%Hct flow in the microchannel,the CFL thickness decrease after being treated.And with concentrations of GA increase,the CFL thickness become thinner.The CFL thickness decrease significantly when GA and 2 wt.%PFA work combined.The velocity of RBCs decreases after treated with the GA or/and 2wt.%PFA.GA is known to relieve the dissolution of red blood cells during fluorescence labeling.On the other hand,the crosslinking of the aldehyde group(-cho)of GA with the amino group(-nh2)of RBC membrane protein will change the conformation of the membrane protein and its visco-elastic properties in turn.Then,the transparent fluidity orrheology characteristics of RBC is altered.Since GA and PFA are commonly used to immobilize red blood cells and keep the fluorescence constant,and PFA works similarly as GA,as a result,the variation of membrane protein conformation is intensified,and the membrane becomes stiffer.
文摘目的:探讨平均红细胞体积(m ean corpuscu lar volum e,MCV)及红细胞体积分布宽度(red b lood cell volum e d istribution w idth,RDW)对地中海贫血(简称地贫,MD)和巨幼细胞性贫血(简称巨幼贫血,MA)诊断的临床价值。方法:利用血细胞自动分析仪测定不同病因的贫血患者的MCV和RDW值。结果:MD患者,MCV降低,RDW升高,且α-地贫与β-地贫无差异;MA患者,MCV和RDW升高;急性失血性贫血和再生障碍性贫血(ap lastic anem ia,AA)患者,MCV和RDW正常。结论:MCV和RDW红细胞参数,可作为贫血病因诊断及鉴别诊断的参考线索,对地贫和巨幼贫血的诊断和治疗有一定的指导意义。
文摘目的:探讨大株红景天联用曲美他嗪对冠心病合并心衰患者疗效及红细胞分布宽度(RDW,Red Blood Cell Distribution Width)、生长分化因子15(GDF-15,Growth and Differentiation factor 15)水平的影响。方法:选择在本院接受治疗的冠心病合并心衰患者104例,随机分为对照组和观察组,每组52例。对照组入院后,根据病情病程给予患者常规的对症治疗:包括血管紧张素转换酶抑制剂或血管紧张素受体拮抗剂、利尿制剂、β受体阻滞剂等药物。观察组在对照组治疗措施的基础上加用大株红景天注射液联合曲美他嗪。疗程结束后,对两组治疗效果展开评估,对患者治疗前后心功能的各项指标进行检测,主要包括:左心室舒张末期内径(LEVDD)、左心室收缩末期内径(LVESD)、左心室射血分数(LVEF)指标;分析比较两组患者治疗前后RDW和GDF-15水平变化。结果:观察组总有效率明显高于对照组(χ~2=8.101,P=0.003);治疗后观察组的LVEF值相比于对照组明显增高,但是观察组LVESD,LVEDD显著低于对照组;治疗后,观察组GDF-15含量显著低于对照组,差异有统计学意义(P<0.05);观察组RDW低于对照组,差异无统计学意义(P>0.05)。结论:大株红景天联用曲美他嗪可有效治疗冠心病合并心衰患者,并能有效降低RDW和GDF-15水平,具有较好的临床应用前景。
文摘目的:探讨红细胞分布宽度(red blood cell distribution width,RDW)、中性粒细胞淋巴细胞比值(neutrophil to lymphocyte ratio,NLR)联合终末期肝病模型(model for end-stage liver disease,MELD)评分评估失代偿期肝硬化患者预后的价值。方法:回顾性分析2013年1月-2016年12月本院确诊的肝硬化失代偿期患者181例,以死亡为终点事件,根据患者随访1年的疾病转归情况分为生存组(n=126)和死亡组(n=55),比较两组患者入院时RDW、NLR、MELD水平差异,运用多因素Cox风险比例模型预测患者1年死亡的独立影响因素,绘制受试者工作特征(ROC)曲线,比较ROC曲线下面积(AUC),应用Kaplan-Meier曲线分析不同RDW、NLR患者的1年生存率变化。最后通过二分类变量Logistic回归对RDW、NLR、MELD评分进行联合,评估多变量联合预测患者1年死亡的价值。结果:181例随访1年死亡55例,存活126例,死亡组入院时NLR、RDW、MELD评分均高于生存组(P<0.05);多因素Cox回归分析后显示,NLR、RDW及MELD评分是预测患者1年死亡的独立危险因素;RDW、NLR及MELD评分预测患者1年预后最佳临界值分别为14.65%、2.58、16.65,ROC曲线下面积(AUC)分别为0.652、0.764、0.862;MELD评分联合NLR的AUC为0.870,MELD评分联合RDW的AUC为0.876,MELD、RDW、NLR三者联合的AUC提高至0.884。结论:MELD、RDW、NLR三者联合对失代偿期肝硬化患者1年预后有较高的预测价值,优于单独MELD评分。
