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Energy-Efficient Deterministic Fault-Tolerant Scheduling for Embedded Real-Time Systems
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作者 李国徽 胡方晓 +1 位作者 杜小坤 唐向红 《Journal of Southwest Jiaotong University(English Edition)》 2009年第4期283-291,共9页
By combining fault-tolerance with power management, this paper developed a new method for aperiodic task set for the problem of task scheduling and voltage allocation in embedded real-time systems. The scbedulability ... By combining fault-tolerance with power management, this paper developed a new method for aperiodic task set for the problem of task scheduling and voltage allocation in embedded real-time systems. The scbedulability of the system was analyzed through checkpointing and the energy saving was considered via dynamic voltage and frequency scaling. Simulation results showed that the proposed algorithm had better performance compared with the existing voltage allocation techniques. The proposed technique saves 51.5% energy over FT-Only and 19.9% over FT + EC on average. Therefore, the proposed method was more appropriate for aperiodic tasks in embedded real-time systems. 展开更多
关键词 Embedded real-time systems Aperiodic tasks Fault tolerance Power management Task scheduling and voltageallocation
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Pore-pressure and stress-coupled creep behavior in deep coal:Insights from real-time NMR analysis 被引量:1
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作者 Wenhao Jia Hongwei Zhou +3 位作者 Senlin Xie Yimeng Wang Xinfeng Hu Lei Zhang 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第1期77-90,共14页
Understanding the variations in microscopic pore-fracture structures(MPFS) during coal creep under pore pressure and stress coupling is crucial for coal mining and effective gas treatment. In this manuscript, a triaxi... Understanding the variations in microscopic pore-fracture structures(MPFS) during coal creep under pore pressure and stress coupling is crucial for coal mining and effective gas treatment. In this manuscript, a triaxial creep test on deep coal at various pore pressures using a test system that combines in-situ mechanical loading with real-time nuclear magnetic resonance(NMR) detection was conducted.Full-scale quantitative characterization, online real-time detection, and visualization of MPFS during coal creep influenced by pore pressure and stress coupling were performed using NMR and NMR imaging(NMRI) techniques. The results revealed that seepage pores and microfractures(SPM) undergo the most significant changes during coal creep, with creep failure gradually expanding from dense primary pore fractures. Pore pressure presence promotes MPFS development primarily by inhibiting SPM compression and encouraging adsorption pores(AP) to evolve into SPM. Coal enters the accelerated creep stage earlier at lower stress levels, resulting in more pronounced creep deformation. The connection between the micro and macro values was established, demonstrating that increased porosity at different pore pressures leads to a negative exponential decay of the viscosity coefficient. The Newton dashpot in the ideal viscoplastic body and the Burgers model was improved using NMR experimental results, and a creep model that considers pore pressure and stress coupling using variable-order fractional operators was developed. The model’s reasonableness was confirmed using creep experimental data. The damagestate adjustment factors ω and β were identified through a parameter sensitivity analysis to characterize the effect of pore pressure and stress coupling on the creep damage characteristics(size and degree of difficulty) of coal. 展开更多
关键词 real-time monitoring Pore pressure-stress coupling Microscopic pore-fracture structure Variable-order fractional creep model Deep coal
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Phase behavior of gas condensate in porous media using real-time computed tomography scanning 被引量:1
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作者 Wen-Long Jing Lei Zhang +5 位作者 Ai-Fen Li Jun-Jie Zhong Hai Sun Yong-Fei Yang Yu-Long Cheng Jun Yao 《Petroleum Science》 SCIE EI CAS CSCD 2024年第2期1032-1043,共12页
The phase behavior of gas condensate in reservoir formations differs from that in pressure-volume-temperature(PVT)cells because it is influenced by porous media in the reservoir formations.Sandstone was used as a samp... The phase behavior of gas condensate in reservoir formations differs from that in pressure-volume-temperature(PVT)cells because it is influenced by porous media in the reservoir formations.Sandstone was used as a sample to investigate the influence of porous media on the phase behavior of the gas condensate.The pore structure was first analyzed using computed tomography(CT)scanning,digital core technology,and a pore network model.The sandstone core sample was then saturated with gas condensate for the pressure depletion experiment.After each pressure-depletion state was stable,realtime CT scanning was performed on the sample.The scanning results of the sample were reconstructed into three-dimensional grayscale images,and the gas condensate and condensate liquid were segmented based on gray value discrepancy to dynamically characterize the phase behavior of the gas condensate in porous media.Pore network models of the condensate liquid ganglia under different pressures were built to calculate the characteristic parameters,including the average radius,coordination number,and tortuosity,and to analyze the changing mechanism caused by the phase behavior change of the gas condensate.Four types of condensate liquid(clustered,branched,membranous,and droplet ganglia)were then classified by shape factor and Euler number to investigate their morphological changes dynamically and elaborately.The results show that the dew point pressure of the gas condensate in porous media is 12.7 MPa,which is 0.7 MPa higher than 12.0 MPa in PVT cells.The average radius,volume,and coordination number of the condensate liquid ganglia increased when the system pressure was between the dew point pressure(12.7 MPa)and the pressure for the maximum liquid dropout,Pmax(10.0 MPa),and decreased when it was below Pmax.The volume proportion of clustered ganglia was the highest,followed by branched,membranous,and droplet ganglia.This study provides crucial experimental evidence for the phase behavior changing process of gas condensate in porous media during the depletion production of gas condensate reservoirs. 展开更多
关键词 Gas condensate Pressure depletion real-time micro-computed tomography scanning Distribution of condensate liquid
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基于Real-time PCR法检测乳粉中牛源性成分定量研究
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作者 陈晨 史国华 +5 位作者 陈勃旭 张瑞 王玉欣 贾文珅 陈佳 周巍 《粮油食品科技》 CAS CSCD 北大核心 2024年第2期159-164,共6页
基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛... 基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛源性成分的相对定量检测。结果显示,该方法的最低检测限为0.00001 mg/mL,回收率为91.11%~119.2%,组间变异系数≤0.58%、组内变异系数≤1.44%。说明该方法在特异性与稳定性上适用于乳粉中牛源性成分及含量的掺假检测。 展开更多
关键词 牛乳粉 马乳粉 real-time PCR 掺假检测
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Real-time data processing method for CO_(2) dispersion interferometer on EAST
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作者 张家敏 姚远 +6 位作者 刘郁阳 储宇奇 阮天翼 张耀 刘海庆 揭银先 凌必利 《Plasma Science and Technology》 SCIE EI CAS CSCD 2024年第8期121-126,共6页
A real-time data processing system is designed for the carbon dioxide dispersion interferometer(CO_(2)-DI)on EAST.The system utilizes the parallel and pipelining capabilities of an fieldprogrammable gate array(FPGA)to... A real-time data processing system is designed for the carbon dioxide dispersion interferometer(CO_(2)-DI)on EAST.The system utilizes the parallel and pipelining capabilities of an fieldprogrammable gate array(FPGA)to digitize and process the intensity of signals from the detector.Finally,the real-time electron density signals are exported through a digital-to-analog converter(DAC)module in the form of analog signals.The system has been successfully applied in the CO_(2)-DI system to provide low-latency electron density input to the plasma control system on EAST.Experimental results of the latest campaign with long-pulse discharges on EAST(2022–2023)demonstrate that the system can respond effectively in the case of rapid density changes,proving its reliability and accuracy for future electron density calculation. 展开更多
关键词 dispersion interferometer real-time electron density FPGA EAST
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Real-Time Tunable Gas Sensing Platform Based on SnO_(2) Nanoparticles Activated by Blue Micro-Light-Emitting Diodes
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作者 Gi Baek Nam Jung-El Ryu +25 位作者 Tae Hoon Eom Seung Ju Kim Jun Min Suh Seungmin Lee Sungkyun Choi Cheon Woo Moon Seon Ju Park Soo Min Lee Byungsoo Kim Sung Hyuk Park Jin Wook Yang Sangjin Min Sohyeon Park Sung Hwan Cho Hyuk Jin Kim Sang Eon Jun Tae Hyung Lee Yeong Jae Kim Jae Young Kim Young Joon Hong Jong-In Shim Hyung-Gi Byun Yongjo Park Inkyu Park Sang-Wan Ryu Ho Won Jang 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第12期103-119,共17页
Micro-light-emitting diodes(μLEDs)have gained significant interest as an activation source for gas sensors owing to their advantages,including room temperature operation and low power consumption.However,despite thes... Micro-light-emitting diodes(μLEDs)have gained significant interest as an activation source for gas sensors owing to their advantages,including room temperature operation and low power consumption.However,despite these benefits,challenges still exist such as a limited range of detectable gases and slow response.In this study,we present a blueμLED-integrated light-activated gas sensor array based on SnO_(2)nanoparticles(NPs)that exhibit excellent sensitivity,tunable selectivity,and rapid detection with micro-watt level power consumption.The optimal power forμLED is observed at the highest gas response,supported by finite-difference time-domain simulation.Additionally,we first report the visible light-activated selective detection of reducing gases using noble metal-decorated SnO_(2)NPs.The noble metals induce catalytic interaction with reducing gases,clearly distinguishing NH3,H2,and C2H5OH.Real-time gas monitoring based on a fully hardwareimplemented light-activated sensing array was demonstrated,opening up new avenues for advancements in light-activated electronic nose technologies. 展开更多
关键词 Micro-LED Gas sensor array Low power consumption Metal decoration real-time detection
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Nanomaterial-assisted wearable glucose biosensors for noninvasive real-time monitoring:Pioneering point-of-care and beyond
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作者 Moein Safarkhani Abdullah Aldhaher +5 位作者 Golnaz Heidari Ehsan Nazarzadeh Zare Majid Ebrahimi Warkiani Omid Akhavan YunSuk Huh Navid Rabiee 《Nano Materials Science》 EI CAS CSCD 2024年第3期263-283,共21页
This review explores glucose monitoring and management strategies,emphasizing the need for reliable and userfriendly wearable sensors that are the next generation of sensors for continuous glucose detection.In additio... This review explores glucose monitoring and management strategies,emphasizing the need for reliable and userfriendly wearable sensors that are the next generation of sensors for continuous glucose detection.In addition,examines key strategies for designing glucose sensors that are multi-functional,reliable,and cost-effective in a variety of contexts.The unique features of effective diabetes management technology are highlighted,with a focus on using nano/biosensor devices that can quickly and accurately detect glucose levels in the blood,improving patient treatment and control of potential diabetes-related infections.The potential of next-generation wearable and touch-sensitive nano biomedical sensor engineering designs for providing full control in assessing implantable,continuous glucose monitoring is also explored.The challenges of standardizing drug or insulin delivery doses,low-cost,real-time detection of increased blood sugar levels in diabetics,and early digital health awareness controls for the adverse effects of injectable medication are identified as unmet needs.Also,the market for biosensors is expected to expand significantly due to the rising need for portable diagnostic equipment and an ever-increasing diabetic population.The paper concludes by emphasizing the need for further research and development of glucose biosensors to meet the stringent requirements for sensitivity and specificity imposed by clinical diagnostics while being cost-effective,stable,and durable. 展开更多
关键词 Glucose sensor BIOSENSOR Wearable devices NONINVASIVE real-time monitoring
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Integrated strategy for real-time wind power fluctuation mitigation and energy storage system control
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作者 Yu Zhang Yongkang Zhang Tiezhou Wu 《Global Energy Interconnection》 EI CSCD 2024年第1期71-81,共11页
To address the impact of wind-power fluctuations on the stability of power systems,we propose a comprehensive approach that integrates multiple strategies and methods to enhance the efficiency and reliability of a sys... To address the impact of wind-power fluctuations on the stability of power systems,we propose a comprehensive approach that integrates multiple strategies and methods to enhance the efficiency and reliability of a system.First,we employ a strategy that restricts long-and short-term power output deviations to smoothen wind power fluctuations in real time.Second,we adopt the sliding window instantaneous complete ensemble empirical mode decomposition with adaptive noise(SW-ICEEMDAN)strategy to achieve real-time decomposition of the energy storage power,facilitating internal power distribution within the hybrid energy storage system.Finally,we introduce a rule-based multi-fuzzy control strategy for the secondary adjustment of the initial power allocation commands for different energy storage components.Through simulation validation,we demonstrate that the proposed comprehensive control strategy can smoothen wind power fluctuations in real time and decompose energy storage power.Compared with traditional empirical mode decomposition(EMD),ensemble empirical mode decomposition(EEMD),and complete ensemble empirical mode decomposition with adaptive noise(CEEMDAN)decomposition strategies,the configuration of the energy storage system under the SW-ICEEMDAN control strategy is more optimal.Additionally,the state-of-charge of energy storage components fluctuates within a reasonable range,enhancing the stability of the power system and ensuring the secure operation of the energy storage system. 展开更多
关键词 SW-ICEEMDAN HESS real-time smoothing Rule-based multi-fuzzy control SoC
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A Framework of LSTM Neural Network Model in Multi-Time Scale Real-Time Prediction of Ship Motions in Head Waves
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作者 CHEN Zhan-yang ZHAN Zheng-yong +2 位作者 CHANG Shao-ping XU Shao-feng LIU Xing-yun 《船舶力学》 EI CSCD 北大核心 2024年第12期1803-1819,共17页
Ship motions induced by waves have a significant impact on the efficiency and safety of offshore operations.Real-time prediction of ship motions in the next few seconds plays a crucial role in performing sensitive act... Ship motions induced by waves have a significant impact on the efficiency and safety of offshore operations.Real-time prediction of ship motions in the next few seconds plays a crucial role in performing sensitive activities.However,the obvious memory effect of ship motion time series brings certain difficulty to rapid and accurate prediction.Therefore,a real-time framework based on the Long-Short Term Memory(LSTM)neural network model is proposed to predict ship motions in regular and irregular head waves.A 15000 TEU container ship model is employed to illustrate the proposed framework.The numerical implementation and the real-time ship motion prediction in irregular head waves corresponding to the different time scales are carried out based on the container ship model.The related experimental data were employed to verify the numerical simulation results.The results show that the proposed method is more robust than the classical extreme short-term prediction method based on potential flow theory in the prediction of nonlinear ship motions. 展开更多
关键词 deep learning LSTM ship motion real-time prediction irregular waves
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A real-time performance improvement method for composite time scale
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作者 Fangmin Wang Wenlin Li +4 位作者 Hongfei Dai Chunyi Li Jianhua Zhou Shenhui Xue Bo Wang 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第9期350-357,共8页
The composite time scale(CTS)provides a stable,accurate,and reliable time scale for modern society.The improvement of CTS’s real-time performance will improve its stability,which strengths related applications’perfo... The composite time scale(CTS)provides a stable,accurate,and reliable time scale for modern society.The improvement of CTS’s real-time performance will improve its stability,which strengths related applications’performance.Aiming at this goal,a method achieved by determining the optimal calculation interval and accelerating adjustment stage is proposed in this paper.The determinants of the CTS’s calculation interval(characteristics of the clock ensemble,the measurement noise,the time and frequency synchronization system’s noise and the auxiliary output generator noise floor)are studied and the optimal calculation interval is obtained.We also investigate the effect of ensemble algorithm’s initial parameters on the CTS’s adjustment stage.A strategy to get the reasonable initial parameters of ensemble algorithm is designed.The results show that the adjustment stage can be finished rapidly or even can be shorten to zero with reasonable initial parameters.On this basis,we experimentally generate a distributed CTS with a calculation interval of 500 s and its stability outperforms those of the member clocks when the averaging time is longer than1700 s.The experimental result proves that the CTS’s real-time performance is significantly improved. 展开更多
关键词 composite time scale real-time performance calculation interval adjustment stage
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FPGA implementation of 500-MHz high-count-rate high-time-resolution real-time digital neutron-gamma discrimination for fast liquid detectors
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作者 Hui‑Yin Shen Jing‑Long Zhang +1 位作者 Jie Zhang Jian‑Hang Zhou 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2024年第8期101-113,共13页
Fast neutron flux measurements with high count rates and high time resolution have important applications in equipment such as tokamaks.In this study,real-time neutron and gamma discrimination was implemented on a sel... Fast neutron flux measurements with high count rates and high time resolution have important applications in equipment such as tokamaks.In this study,real-time neutron and gamma discrimination was implemented on a self-developed 500-Msps,12-bit digitizer,and the neutron and gamma spectra were calculated directly on an FPGA.A fast neutron flux measurement system with BC-501A and EJ-309 liquid scintillator detectors was developed and a fast neutron measurement experiment was successfully performed on the HL-2 M tokamak at the Southwestern Institute of Physics,China.The experimental results demonstrated that the system obtained the neutron and gamma spectra with a time accuracy of 1 ms.At count rates of up to 1 Mcps,the figure of merit was greater than 1.05 for energies between 50 keV and 2.8 MeV. 展开更多
关键词 Neutron-gamma discrimination Liquid scintillation detector real-time spectrum analyzer
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24重荧光real-time PCR技术在食物中毒快速检测中的应用
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作者 卢媛 钟颖涛 《食品安全导刊》 2024年第6期85-88,共4页
目的:应用24重荧光real-time PCR检测技术快速筛检食物中毒病原菌,结合国家标准中的培养法探讨24重荧光real-time PCR检测技术符合性和应用价值。方法:采用高灵敏度、高特异性的24重荧光real-time PCR检测技术作为中毒病原菌的初筛方法... 目的:应用24重荧光real-time PCR检测技术快速筛检食物中毒病原菌,结合国家标准中的培养法探讨24重荧光real-time PCR检测技术符合性和应用价值。方法:采用高灵敏度、高特异性的24重荧光real-time PCR检测技术作为中毒病原菌的初筛方法,国标方法进行细菌分离培养,并对分离出的病原菌进行生化鉴定。结果:5份食物中毒患者肛拭子在增菌前检出4份霍乱弧菌核酸(非O1/非O139群,24重荧光real-time PCR),9份患者肛拭子在增菌后检出5株霍乱弧菌(非O1/非O139群,国标培养法),其中包含4份PCR技术初筛阳性样品,两个方法的符合率为80%。所有样本均未检出沙门氏菌、志贺菌、副溶血性弧菌、致泻性大肠埃希菌以及金黄色葡萄球菌。结论:应用24重荧光real-time PCR检测技术同时检测24种常见致病病原菌,能高效锁定中毒病原菌。将其与国标培养法相结合,对临床治疗和食物中毒快速处置能起到积极作用,值得应用和推广。 展开更多
关键词 24重荧光real-time PCR技术 培养法 食物中毒
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用DGGE和Real-Time PCR对低温沼气池中产甲烷古菌群落的研究 被引量:23
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作者 王彦伟 徐凤花 +3 位作者 阮志勇 宋金龙 王庆 赵斌 《中国沼气》 北大核心 2012年第1期8-12,共5页
利用DGGE及Real-Time PCR技术对不同地区低温沼气池中沼泥行进发酵前期、后期产甲烷古菌群落变化进行了研究。DGGE技术用于分析发酵前后产甲烷古菌优势群落的变化;Real-Time PCR技术用于分析发酵前期、后期样品产甲烷古菌的数量变化。... 利用DGGE及Real-Time PCR技术对不同地区低温沼气池中沼泥行进发酵前期、后期产甲烷古菌群落变化进行了研究。DGGE技术用于分析发酵前后产甲烷古菌优势群落的变化;Real-Time PCR技术用于分析发酵前期、后期样品产甲烷古菌的数量变化。结果表明,沼气低温发酵的过程中,不同沼泥样品发酵前期、后期产甲烷古菌的优势群落差异明显且数量上也存在较大差异;主要变化的产甲烷古菌的优势群落为甲烷粒菌属(Methanocor-pusculum),甲烷八叠球菌属(Methanosarcina),甲烷鬃毛菌属(Methanosaeta)。甲烷八叠球菌属(Methanosarcina)在黑龙江沼泥样品(A1)发酵后期成为优势群落,甲烷鬃毛菌属(Methanosaeta)在山东发酵前期(B1),后期样品(B2)及安徽发酵前期样品(C1)中占有绝对优势。产甲烷古菌的数量介于104~105个.mL-1之间,其中黑龙江样品中产甲烷菌数量最多,而安徽样品中产甲烷菌数量最少。 展开更多
关键词 沼泥 产甲烷古菌 DGGE real-time PCR
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运用Real-time quantification PCR方法建立副溶血性弧菌在即食虾中的生长预测模型 被引量:5
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作者 彭织云 王敬敬 +2 位作者 唐晓阳 潘迎捷 赵勇 《食品工业科技》 CAS CSCD 北大核心 2013年第8期108-110,共3页
运用Real-time quantification PCR(real-time qPCR)方法建立副溶血性弧菌在即食虾中生长预测模型。首先构建质粒标准品,梯度稀释后建立标准曲线,然后用Real-time qPCR方法检测虾中副溶血性弧菌的数量,最后建立37℃下即食虾中副溶血性... 运用Real-time quantification PCR(real-time qPCR)方法建立副溶血性弧菌在即食虾中生长预测模型。首先构建质粒标准品,梯度稀释后建立标准曲线,然后用Real-time qPCR方法检测虾中副溶血性弧菌的数量,最后建立37℃下即食虾中副溶血性弧菌生长预测模型,并与传统涂布计数方法进行比较。结果表明,Real-time qPCR方法和传统计数方法均可建立Gmopertz模型、Logistic模型和Richards模型,模型拟合的相关系数R2均在0.9以上。基于Real-timeqPCR方法省时省力、特异性好等优点,用Real-time qPCR方法建立微生物预测模型是未来预测微生物学领域的一种发展趋势。 展开更多
关键词 real-time quantification PCR 副溶血性弧菌 生长预测模型
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利用SYBR Green检测衣原体Real-Time PCR方法的建立 被引量:2
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作者 杨建民 郝永新 +1 位作者 赵德明 何诚 《畜牧兽医学报》 CAS CSCD 北大核心 2006年第1期84-90,共7页
利用SYBR Green建立了检测种特异性衣原体的Real-Time PCR方法。本方法应用衣原体种特异性的高度保守特异引物,能够扩增627bp特异片段;使用定量标准基因组DNA,本方法能准确检测最少250fg衣原体DNA。Real-TimePCR方法与免疫荧光方法... 利用SYBR Green建立了检测种特异性衣原体的Real-Time PCR方法。本方法应用衣原体种特异性的高度保守特异引物,能够扩增627bp特异片段;使用定量标准基因组DNA,本方法能准确检测最少250fg衣原体DNA。Real-TimePCR方法与免疫荧光方法的检测结果表明:检测4种衣原体临床样本,Real-TimePCR敏感性均在96%~98%;特异性均为100%;这两种方法符合率达97%以上(n=60);批内和批间重复性试验结果表明,本方法具有良好的准确性。本方法的建立对于快速、准确检测临床样本种特异性衣原体提供了一种切实有效的方法。 展开更多
关键词 SYBR Green 衣原体 real-time PCR 检测
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猴逆转录病毒RT-PCR和Real-time RT-PCR检测方法的建立 被引量:2
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作者 熊炜 蒋静 +5 位作者 张强 盘宝进 李健 魏晓锋 黄忠荣 胡建华 《动物医学进展》 CSCD 北大核心 2013年第12期51-54,共4页
猴逆转录病毒(Simian type D retrovirus,SRV)是引起猴获得性免疫缺陷综合征(Simian acquired immunodeficiency syndrome,SAIDS)的病原之一,其严重危害猴的健康,并威胁与猴接触人员的健康,是无特定病原体(SPF)猴必须排除的病毒之一。... 猴逆转录病毒(Simian type D retrovirus,SRV)是引起猴获得性免疫缺陷综合征(Simian acquired immunodeficiency syndrome,SAIDS)的病原之一,其严重危害猴的健康,并威胁与猴接触人员的健康,是无特定病原体(SPF)猴必须排除的病毒之一。为了应对口岸对进出境野生及实验用灵长类动物SRV感染情况的监测和流行病学调查的需要,建立了RT-PCR和real-time RT-PCR检测SRV的方法,并对方法的特异性、敏感性和稳定性进行了验证。 展开更多
关键词 猴逆转录病毒 RT-PCR real-time RT-PCR TAQ Man探针
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猪PD-1及其配体PD-L1和PD-L2 SYBR Green Ⅰ Real-time PCR检测方法的建立及应用 被引量:2
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作者 岳锋 周娟娟 +3 位作者 朱艳平 李鹏 孙国鹏 王选年 《中国畜牧兽医》 CAS 北大核心 2016年第11期2892-2899,共8页
本试验旨在研究猪PD-1及其配体在疾病状态下的转录水平,建立检测猪PD-1及其配体的Real-time PCR方法。根据GenBank中猪PD-1、PD-L1和PD-L2基因序列,分别设计3对特异性引物,PCR扩增目的基因片段,回收目的基因片段与pMD18-T连接后转化至... 本试验旨在研究猪PD-1及其配体在疾病状态下的转录水平,建立检测猪PD-1及其配体的Real-time PCR方法。根据GenBank中猪PD-1、PD-L1和PD-L2基因序列,分别设计3对特异性引物,PCR扩增目的基因片段,回收目的基因片段与pMD18-T连接后转化至大肠杆菌DH5α感受态细胞中,提取重组质粒,经PCR及测序鉴定正确,作为标准品绘制标准曲线,并进行特异性和重复性检测。结果显示,Real-time PCR的Ct值与模板浓度对数呈良好的线性反比关系,相关系数R2>0.99,熔解曲线出现狭窄单一峰;Real-time PCR产物经琼脂糖凝胶电泳,呈单一目的条带,无引物二聚体,特异性强;组内和组间重复性检测结果显示,组内和组间的变异系数均小于3%,重复性好;用建立的Real-time PCR方法检测猪自然感染PCV2后PD-1及其配体转录水平,PD-L1和PD-L2转录水平极显著或显著升高(P<0.01;P<0.05),PD-1转录水平无显著变化(P>0.05)。本试验建立了检测猪PD-1、PDL1和PD-L2mRNA的Real-time PCR方法并进行了初步应用,为猪PD-1、PD-L1和PD-L2基因表达模式研究奠定基础。 展开更多
关键词 real-time PCR PD-1 PD-L1 PD-L2
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COMPENSATION CONTROL OF REAL-TIME UNBALANCE FORCE FOR ACTIVE MAGNETIC BEARING SYSTEM 被引量:3
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作者 高辉 徐龙祥 朱益利 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2011年第2期183-191,共9页
Aiming at the stability and others properties of active magnetic bearing (AMB) system influenced by the periodic unbalance stimulation synchronous with rotor rotational speed, a new real-time adaptive feed-forward u... Aiming at the stability and others properties of active magnetic bearing (AMB) system influenced by the periodic unbalance stimulation synchronous with rotor rotational speed, a new real-time adaptive feed-forward unbalance force compensation scheme is proposed based on variable step-size least mean square(LMS) algorithm as the feed-forward compensation controller. The controller can provide some suitable sinusoidal signals to com- pensate the feedback unbalance response signals synchronous with the rotary frequency, then reduce the fluctua- tion of the control currents and weaken the active control of AMB system. The variable step-size proportional to the rotational frequency is deduced by analyzing the principle of normal LMS algorithm and its deficiency in the application of real-time filtering of AMB system. Experimental results show that the new method can implement real-time unbalance force compensation in a wide frequency band, reduce the effect of unbalance stimulant force on the housing of AMB system, and provide convenience to improve rotational speed. 展开更多
关键词 active magnetic bearing variable step-size LMS algorithm bandwidth~ real-time unbalance forcecompensation~ adaptive filter
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REAL-TIME TRACKING FOR FAST MOVING OBJECT ON COMPLEX BACKGROUND 被引量:3
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作者 张超 王道波 Farooq M 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2010年第4期321-325,共5页
A real-time tracking system for the fast moving object on the complex background is proposed.The Markov random filed(MRF)model based background subtraction algorithm is used to detect the changing pixels and track t... A real-time tracking system for the fast moving object on the complex background is proposed.The Markov random filed(MRF)model based background subtraction algorithm is used to detect the changing pixels and track the moving object.The prior probability of the segmentation mask is modeled by using MRF,and the object tracking task is translated into the maximum a-posterior(MAP)problem.Experimental results show that the method is efficient at both offline and online moving objects on simple and complex background. 展开更多
关键词 unmanned aerial vechicles real-time tracking Markov random field background subtraction
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Data network traffic analysis and optimization strategy of real-time power grid dynamic monitoring system for wide-frequency measurements 被引量:4
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作者 Jinsong Li Hao Liu +2 位作者 Wenzhuo Li Tianshu Bi Mingyang Zhao 《Global Energy Interconnection》 EI CAS CSCD 2022年第2期131-142,共12页
The application and development of a wide-area measurement system(WAMS)has enabled many applications and led to several requirements based on dynamic measurement data.Such data are transmitted as big data information ... The application and development of a wide-area measurement system(WAMS)has enabled many applications and led to several requirements based on dynamic measurement data.Such data are transmitted as big data information flow.To ensure effective transmission of wide-frequency electrical information by the communication protocol of a WAMS,this study performs real-time traffic monitoring and analysis of the data network of a power information system,and establishes corresponding network optimization strategies to solve existing transmission problems.This study utilizes the traffic analysis results obtained using the current real-time dynamic monitoring system to design an optimization strategy,covering the optimization in three progressive levels:the underlying communication protocol,source data,and transmission process.Optimization of the system structure and scheduling optimization of data information are validated to be feasible and practical via tests. 展开更多
关键词 Power system Data network Wide-frequency information real-time system Traffic analysis Optimization strategy
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