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Influencing factor of the characterization and restoration of phase aberrations resulting from atmospheric turbulence based on Principal Component Analysis
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作者 WANG Jiang-pu-zhen WANG Zhi-qiang +2 位作者 ZHANG Jing-hui QIAO Chun-hong FAN Cheng-yu 《中国光学(中英文)》 北大核心 2025年第4期899-907,共9页
Restoration of phase aberrations is crucial for addressing atmospheric turbulence in light propagation.Traditional restoration algorithms based on Zernike polynomials(ZPs)often encounter challenges related to high com... Restoration of phase aberrations is crucial for addressing atmospheric turbulence in light propagation.Traditional restoration algorithms based on Zernike polynomials(ZPs)often encounter challenges related to high computational complexity and insufficient capture of high-frequency phase aberration components,so we proposed a Principal-Component-Analysis-based method for representing phase aberrations.This paper discusses the factors influencing the accuracy of restoration,mainly including the sample space size and the sampling interval of D/r_(0),on the basis of characterizing phase aberrations by Principal Components(PCs).The experimental results show that a larger D/r_(0)sampling interval can ensure the generalization ability and robustness of the principal components in the case of a limited amount of original data,which can help to achieve high-precision deployment of the model in practical applications quickly.In the environment with relatively strong turbulence in the test set of D/r_(0)=24,the use of 34 terms of PCs can improve the corrected Strehl ratio(SR)from 0.007 to 0.1585,while the Strehl ratio of the light spot after restoration using 34 terms of ZPs is only 0.0215,demonstrating almost no correction effect.The results indicate that PCs can serve as a better alternative in representing and restoring the characteristics of atmospheric turbulence induced phase aberrations.These findings pave the way to use PCs of phase aberrations with fewer terms than traditional ZPs to achieve data dimensionality reduction,and offer a reference to accelerate and stabilize the model and deep learning based adaptive optics correction. 展开更多
关键词 phase aberration atmospheric turbulence principal component analysis Zernike polynomials
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Research on multi-scale simulation and dynamic verification of high dynamic MEMS components in additive manufacturing
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作者 Sining Lv Hengzhen Feng +2 位作者 Wenzhong Lou Chuan Xiao Shiyi Li 《Defence Technology(防务技术)》 2025年第5期275-291,共17页
Metal Additive Manufacturing(MAM) technology has become an important means of rapid prototyping precision manufacturing of special high dynamic heterogeneous complex parts. In response to the micromechanical defects s... Metal Additive Manufacturing(MAM) technology has become an important means of rapid prototyping precision manufacturing of special high dynamic heterogeneous complex parts. In response to the micromechanical defects such as porosity issues, significant deformation, surface cracks, and challenging control of surface morphology encountered during the selective laser melting(SLM) additive manufacturing(AM) process of specialized Micro Electromechanical System(MEMS) components, multiparameter optimization and micro powder melt pool/macro-scale mechanical properties control simulation of specialized components are conducted. The optimal parameters obtained through highprecision preparation and machining of components and static/high dynamic verification are: laser power of 110 W, laser speed of 600 mm/s, laser diameter of 75 μm, and scanning spacing of 50 μm. The density of the subordinate components under this reference can reach 99.15%, the surface hardness can reach 51.9 HRA, the yield strength can reach 550 MPa, the maximum machining error of the components is 4.73%, and the average surface roughness is 0.45 μm. Through dynamic hammering and high dynamic firing verification, SLM components meet the requirements for overload resistance. The results have proven that MEM technology can provide a new means for the processing of MEMS components applied in high dynamic environments. The parameters obtained in the conclusion can provide a design basis for the additive preparation of MEMS components. 展开更多
关键词 Additive manufacturing High dynamic MEMS components Multiscale control Process optimization High dynamic verification
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Real-time lane departure warning system based on principal component analysis of grayscale distribution and risk evaluation model 被引量:4
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作者 张伟伟 宋晓琳 张桂香 《Journal of Central South University》 SCIE EI CAS 2014年第4期1633-1642,共10页
A technology for unintended lane departure warning was proposed. As crucial information, lane boundaries were detected based on principal component analysis of grayscale distribution in search bars of given number and... A technology for unintended lane departure warning was proposed. As crucial information, lane boundaries were detected based on principal component analysis of grayscale distribution in search bars of given number and then each search bar was tracked using Kalman filter between frames. The lane detection performance was evaluated and demonstrated in ways of receiver operating characteristic, dice similarity coefficient and real-time performance. For lane departure detection, a lane departure risk evaluation model based on lasting time and frequency was effectively executed on the ARM-based platform. Experimental results indicate that the algorithm generates satisfactory lane detection results under different traffic and lighting conditions, and the proposed warning mechanism sends effective warning signals, avoiding most false warning. 展开更多
关键词 lane departure warning system lane detection lane tracking principal component analysis risk evaluation model ARM-based real-time system
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Effects of main components on energy output characteristics of thermobaric explosive——A case study of typical formulations 被引量:1
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作者 Yunfei Zhao Yaning Li +3 位作者 Zhiwei Han Peng Bao Jingyan Wang Boliang Wang 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第8期205-216,共12页
As a kind of high-efficiency explosive with compound destructive capability, the energy output law of thermobaric explosives has been receiving great attention. In order to investigate the effects of main components o... As a kind of high-efficiency explosive with compound destructive capability, the energy output law of thermobaric explosives has been receiving great attention. In order to investigate the effects of main components on the explosive characteristics of thermobaric explosives, various high explosives and oxidants were selected to formulate five different types of thermobaric explosive. Then they were tested in both open space and closed space respectively. Pressure measurement system, high-speed camera,infrared thermal imager and multispectral temperature measurement system were used for pressure,temperature and fireball recording. The effects of different components on the explosive characteristics of thermobaric explosive were analyzed. The results showed that in open space, the overpressure is dominated by the high explosives content in the formulation. The addition of the oxidants will decrease the explosion overpressure but will increase the duration and overall brightness of the fireball. While in closed space, the quasi-static pressure formed after the explosion is positively correlated with the temperature and gas production. In addition, it was found that the differences in shell constraints can also alter the afterburning reaction of thermobaric explosives, thus affecting their energy output characteristics. PVC shell constraint obviously increases the overpressure and makes the fireball burn more violently. 展开更多
关键词 Thermobaric explosives componentS OVERPRESSURE FIREBALL Afterburning reaction
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基于Real-time PCR法检测乳粉中牛源性成分定量研究
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作者 陈晨 史国华 +5 位作者 陈勃旭 张瑞 王玉欣 贾文珅 陈佳 周巍 《粮油食品科技》 CAS CSCD 北大核心 2024年第2期159-164,共6页
基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛... 基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛源性成分的相对定量检测。结果显示,该方法的最低检测限为0.00001 mg/mL,回收率为91.11%~119.2%,组间变异系数≤0.58%、组内变异系数≤1.44%。说明该方法在特异性与稳定性上适用于乳粉中牛源性成分及含量的掺假检测。 展开更多
关键词 牛乳粉 马乳粉 real-time PCR 掺假检测
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A Framework of LSTM Neural Network Model in Multi-Time Scale Real-Time Prediction of Ship Motions in Head Waves 被引量:1
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作者 CHEN Zhan-yang ZHAN Zheng-yong +2 位作者 CHANG Shao-ping XU Shao-feng LIU Xing-yun 《船舶力学》 EI CSCD 北大核心 2024年第12期1803-1819,共17页
Ship motions induced by waves have a significant impact on the efficiency and safety of offshore operations.Real-time prediction of ship motions in the next few seconds plays a crucial role in performing sensitive act... Ship motions induced by waves have a significant impact on the efficiency and safety of offshore operations.Real-time prediction of ship motions in the next few seconds plays a crucial role in performing sensitive activities.However,the obvious memory effect of ship motion time series brings certain difficulty to rapid and accurate prediction.Therefore,a real-time framework based on the Long-Short Term Memory(LSTM)neural network model is proposed to predict ship motions in regular and irregular head waves.A 15000 TEU container ship model is employed to illustrate the proposed framework.The numerical implementation and the real-time ship motion prediction in irregular head waves corresponding to the different time scales are carried out based on the container ship model.The related experimental data were employed to verify the numerical simulation results.The results show that the proposed method is more robust than the classical extreme short-term prediction method based on potential flow theory in the prediction of nonlinear ship motions. 展开更多
关键词 deep learning LSTM ship motion real-time prediction irregular waves
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一种基于real-time PCR技术的TTV检测方法的建立及应用
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作者 贾毅博 王高玉 +4 位作者 邓宛心 林彩云 杨华 陈运春 尹飞飞 《海南医学院学报》 CAS 北大核心 2024年第7期489-497,共9页
目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域... 目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域的real-time PCR检测方法,并与文献报道应用较为广泛的PCR检测方法进行了对比。结果:本研究建立的方法在1×10^(7)~1×10^(1) copies/μL标准品浓度范围内具有良好的线性关系,相关系数为1.000,斜率为-3.446,检测下限为1×10^(1) copies/μL。重复性试验结果显示,组内变异系数为7.22%,表明本方法重复性、稳定性较强。针对30份临床样本,使用本研究建立的real-time PCR检测方法及目前被多个研究所使用的4套引物进行对比。结果表明,本研究所建立的方法灵敏度显著高于文献中报道的4种方法(P<0.01);Sanger测序结果表明,本方法检测出的30份阳性样本均为TTV,检测特异性为100%。结论:本研究采用基于TaqMan探针的real-time PCR检测方法,检测灵敏性高、覆盖基因型范围广,尤其对于TTV病毒载量较低的情况下能够进行定量检测,对于TTV病毒的致病性及作为免疫标志物的应用提供重要的技术支持。 展开更多
关键词 Torque teno virus 基因组扩增测序 real-time PCR检测
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Redox mechanism of geobattery and related electrical signals using a novel real-time self-potential monitoring experimental platform 被引量:1
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作者 XIE Jing CUI Yi-an +4 位作者 ZHANG Li-juan GUO You-jun CHEN Hang ZHANG Peng-fei LIU Jian-xin 《Journal of Central South University》 CSCD 2024年第11期4155-4173,共19页
Controlled laboratory experiments are proved to be a valuable tool for investigating changes in underground physical properties and the related response of surface geophysical signals.The self-potential(SP)method is w... Controlled laboratory experiments are proved to be a valuable tool for investigating changes in underground physical properties and the related response of surface geophysical signals.The self-potential(SP)method is widely used in mineral resource exploration due to its direct correlation with underground electrochemical gradients.This paper presented the design and construction of an experimental platform based on a multi-channel SP monitoring system.The proposed platform was used to monitor the anodizing corrosion process of different metal blocks from a laboratory perspective,record the real-time SP signal generated by the redox reaction,as well as investigate the geobattery mechanism associated with the natural polarization process of metal mineral resources.The experimental results demonstrate that the constructed SP monitoring platform effectively captures time-series SP signals and provides direct laboratory evidence for the geobattery model.The measured SP data were quantitatively interpreted using the simulated annealing algorithm,and the inversion results closely match the real model.This finding highlights the potential of the SP method as a promising tool for determining the location and spatial distribution of underground polarizers.The study holds reference value for the exploration and exploitation of mineral resources in both terrestrial and marine environments. 展开更多
关键词 SELF-POTENTIAL real-time monitoring laboratory experiment geobattery mechanism quantitative inversion
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Real-Time Monitoring Method for Cow Rumination Behavior Based on Edge Computing and Improved MobileNet v3
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作者 ZHANG Yu LI Xiangting +4 位作者 SUN Yalin XUE Aidi ZHANG Yi JIANG Hailong SHEN Weizheng 《智慧农业(中英文)》 CSCD 2024年第4期29-41,共13页
[Objective]Real-time monitoring of cow ruminant behavior is of paramount importance for promptly obtaining relevant information about cow health and predicting cow diseases.Currently,various strategies have been propo... [Objective]Real-time monitoring of cow ruminant behavior is of paramount importance for promptly obtaining relevant information about cow health and predicting cow diseases.Currently,various strategies have been proposed for monitoring cow ruminant behavior,including video surveillance,sound recognition,and sensor monitoring methods.How‐ever,the application of edge device gives rise to the issue of inadequate real-time performance.To reduce the volume of data transmission and cloud computing workload while achieving real-time monitoring of dairy cow rumination behavior,a real-time monitoring method was proposed for cow ruminant behavior based on edge computing.[Methods]Autono‐mously designed edge devices were utilized to collect and process six-axis acceleration signals from cows in real-time.Based on these six-axis data,two distinct strategies,federated edge intelligence and split edge intelligence,were investigat‐ed for the real-time recognition of cow ruminant behavior.Focused on the real-time recognition method for cow ruminant behavior leveraging federated edge intelligence,the CA-MobileNet v3 network was proposed by enhancing the MobileNet v3 network with a collaborative attention mechanism.Additionally,a federated edge intelligence model was designed uti‐lizing the CA-MobileNet v3 network and the FedAvg federated aggregation algorithm.In the study on split edge intelli‐gence,a split edge intelligence model named MobileNet-LSTM was designed by integrating the MobileNet v3 network with a fusion collaborative attention mechanism and the Bi-LSTM network.[Results and Discussions]Through compara‐tive experiments with MobileNet v3 and MobileNet-LSTM,the federated edge intelligence model based on CA-Mo‐bileNet v3 achieved an average Precision rate,Recall rate,F1-Score,Specificity,and Accuracy of 97.1%,97.9%,97.5%,98.3%,and 98.2%,respectively,yielding the best recognition performance.[Conclusions]It is provided a real-time and effective method for monitoring cow ruminant behavior,and the proposed federated edge intelligence model can be ap‐plied in practical settings. 展开更多
关键词 cow rumination behavior real-time monitoring edge computing improved MobileNet v3 edge intelligence model Bi-LSTM
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Identification of Active Components in Humic Acids Affecting Tomato Germination
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作者 Wei Shiping Shi Peihua +4 位作者 Wang Quanzhi Wang Yuanhua Zhao Jiayin Yin Kaijun Wu Meng 《Journal of Northeast Agricultural University(English Edition)》 CAS 2024年第4期1-15,共15页
Humic acids can promote the germination of many vegetable seeds,but the key active components remain unclear.This study utilized nutrient content,cross polarization magic angle spin ^(13)C solid magnetic resonance(CPM... Humic acids can promote the germination of many vegetable seeds,but the key active components remain unclear.This study utilized nutrient content,cross polarization magic angle spin ^(13)C solid magnetic resonance(CPMAS-^(13)C-NMR)and ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)to characterize the chemical components of humic acids.Tomato seed germination index(GI)was determined with the goal of screening the key active components of humic acids.Humic acids had a significantly higher nutrient content,except for the total nitrogen(TN)and the total phosphorus(TP)contents.Humic acids had a higher content of O-CH_(3)/NCH,aromatic C-O and carbonyl C compared to weathered coal,with significantly lower anomeric C,aromatic C and O-alkyl C/alkyl C.There were 611 different compounds identified among the test materials using UHPLC-MS.Humic acids also had a significantly higher GI(158.0%and 153.1%)than weathered coal(85.5%).The organic matter(OM),TP and available potassium(AK)contents in humic acids were significantly positively correlated with GI,and available phosphorus(AP)was significantly negatively correlated.Among the carbon components,O-CH3/NCH,aromatic C-O and O-alkyl C/alkyl C were significantly positively correlated with GI,while anomeric C was significantly negatively correlated.Furthermore,among the top 10 positive and five negative correlation compounds,lipids and lipid-like molecules[armexifolin,boviquinone 4,3-methyladipic acid,lxocarpalactone A,monic acid,DG(20:1(11Z)/18:4(6Z,9Z,12Z,15Z)/0:0),and brassinolide]and organic acids and derivatives(N-acetylglutamic acid,8-hydroxy-5,6-octadienoic acid,acetyl-L-tyrosine,and hydroxyprolyl-methionine)in humic acids might be crucial active components for improving tomato seed germination.The results provided direct evidence for the identification of bioactive molecules of humic acids,and a scientific basis for the precise utilization of bioactive molecular components of humic acids in sustainable agricultural development. 展开更多
关键词 humic acid cross polarization magic angle spin 13C solid magnetic resonance(CPMAS-13C-NMR) ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS) germination index(GI) active component
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运用Real-time PCR方法研究日粮添加豆油与胡麻油对肉牛瘤胃纤维分解菌数量的影响 被引量:13
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作者 李旦 王加启 +3 位作者 卜登攀 杨舒黎 魏宏阳 周凌云 《动物营养学报》 CAS CSCD 北大核心 2008年第3期256-260,共5页
本研究分别以产琥珀酸丝状杆菌(Fibrobacter succinogenes)、黄色瘤胃球菌(Ruminococcus flavefaciens)、白色瘤胃球菌(Ruminobacter albus)和溶纤维丁酸弧菌(Butyrivibrio fibrisolvens)16S rDNA序列设计引物,运用Real-ti me PCR技术... 本研究分别以产琥珀酸丝状杆菌(Fibrobacter succinogenes)、黄色瘤胃球菌(Ruminococcus flavefaciens)、白色瘤胃球菌(Ruminobacter albus)和溶纤维丁酸弧菌(Butyrivibrio fibrisolvens)16S rDNA序列设计引物,运用Real-ti me PCR技术研究日粮中添加豆油与胡麻油对肉牛上述4种瘤胃纤维分解菌数量的影响。结果表明,与对照组(CK)相比,添加豆油组(LOC1)和胡麻油(LOC2)组,产琥珀酸丝状杆菌(Fibrobacter succinogenes)、黄色瘤胃球菌(Ruminococcus flavefaciens)、白色瘤胃球菌(Ruminobacter albus)和溶纤维丁酸弧菌(Butyrivibrio fibrisol-vens)数量显著减少(P<0.05),分别降低了78%和31%、30%和36%、27%和23%、6%和13%。通过该方法的结果表明日粮中添加4%的豆油和胡麻油显著减少了瘤胃中纤维分解菌,对产琥珀酸丝状杆菌的影响明显。而且采用Real-ti me PCR方法对瘤胃纤维分解菌进行定量,可以快速有效反映出在日粮改变的情况下菌的数量变化趋势,相对于传统计数方法更直观、快捷与准确。 展开更多
关键词 real-time PCR 瘤胃纤维菌 定量 肉牛
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非洲猪瘟病毒常规PCR及Real-time PCR检测方法的建立 被引量:22
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作者 张泉 朱鸿飞 孙怀昌 《中国预防兽医学报》 CAS CSCD 北大核心 2007年第6期458-461,共4页
根据非洲猪瘟病毒(African swine fever virus,ASFV)P72基因的核苷酸序列,设计并合成引物以及荧光标记的TaqMan探针,以含P72基因的重组质粒作为阳性模板,用于常规PCR和Real-time PCR方法的建立,结果表明常规PCR的检测灵敏度是600个拷贝... 根据非洲猪瘟病毒(African swine fever virus,ASFV)P72基因的核苷酸序列,设计并合成引物以及荧光标记的TaqMan探针,以含P72基因的重组质粒作为阳性模板,用于常规PCR和Real-time PCR方法的建立,结果表明常规PCR的检测灵敏度是600个拷贝的病毒核酸分子,Real-time PCR的检测灵敏度是20个拷贝的病毒核酸分子,两种PCR检测方法均具有特异性强、简单快速的优点。可以用于出入境检验检疫部门对非洲猪瘟病毒的快速检测。 展开更多
关键词 非洲猪瘟 常规PCR real-time PCR
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用DGGE和Real-Time PCR对低温沼气池中产甲烷古菌群落的研究 被引量:23
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作者 王彦伟 徐凤花 +3 位作者 阮志勇 宋金龙 王庆 赵斌 《中国沼气》 北大核心 2012年第1期8-12,共5页
利用DGGE及Real-Time PCR技术对不同地区低温沼气池中沼泥行进发酵前期、后期产甲烷古菌群落变化进行了研究。DGGE技术用于分析发酵前后产甲烷古菌优势群落的变化;Real-Time PCR技术用于分析发酵前期、后期样品产甲烷古菌的数量变化。... 利用DGGE及Real-Time PCR技术对不同地区低温沼气池中沼泥行进发酵前期、后期产甲烷古菌群落变化进行了研究。DGGE技术用于分析发酵前后产甲烷古菌优势群落的变化;Real-Time PCR技术用于分析发酵前期、后期样品产甲烷古菌的数量变化。结果表明,沼气低温发酵的过程中,不同沼泥样品发酵前期、后期产甲烷古菌的优势群落差异明显且数量上也存在较大差异;主要变化的产甲烷古菌的优势群落为甲烷粒菌属(Methanocor-pusculum),甲烷八叠球菌属(Methanosarcina),甲烷鬃毛菌属(Methanosaeta)。甲烷八叠球菌属(Methanosarcina)在黑龙江沼泥样品(A1)发酵后期成为优势群落,甲烷鬃毛菌属(Methanosaeta)在山东发酵前期(B1),后期样品(B2)及安徽发酵前期样品(C1)中占有绝对优势。产甲烷古菌的数量介于104~105个.mL-1之间,其中黑龙江样品中产甲烷菌数量最多,而安徽样品中产甲烷菌数量最少。 展开更多
关键词 沼泥 产甲烷古菌 DGGE real-time PCR
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一种莱姆病螺旋体real-time PCR方法的建立及其在鼠标本检测中的应用评价(英文) 被引量:12
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作者 耿震 侯学霞 +1 位作者 张琳 郝琴 《中国人兽共患病学报》 CAS CSCD 北大核心 2015年第9期812-816,共5页
目的基于莱姆病螺旋体recA基因,建立一种检测鼠中莱姆病螺旋体的real-time PCR方法。方法通过GenBank分析比较莱姆病螺旋体recA基因,选择其保守序列设计MGB探针及引物并进行方法学评估。并应用建立的real-time PCR方法和nested PCR方法... 目的基于莱姆病螺旋体recA基因,建立一种检测鼠中莱姆病螺旋体的real-time PCR方法。方法通过GenBank分析比较莱姆病螺旋体recA基因,选择其保守序列设计MGB探针及引物并进行方法学评估。并应用建立的real-time PCR方法和nested PCR方法对收集的123份鼠标本进行检测分析。结果本研究建立的real-time PCR方法仅对莱姆病螺旋体检测阳性,其最小检出浓度为101copies/μL。标准曲线各浓度点Ct值批内、批间平均变异系数(CV)分别为1.56%和2.30%。123份鼠标本中,real-time PCR检测59例阳性,nested PCR检测43例阳性。结论新建立的real-time PCR方法具有快速、敏感和特异的优点,可用于鼠标本中莱姆病螺旋体的检测。 展开更多
关键词 莱姆病螺旋体 real-time PCR nested PCR
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Real-time PCR方法检测肉品中的沙门氏菌 被引量:16
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作者 方平 杨永莉 +1 位作者 杨宝 王晓闻 《山西农业科学》 2010年第8期71-76,共6页
应用SYBR Green I染料能选择性结合双链DNA的特点,可检测到沙门氏菌fimI基因特异性靶序列扩增所产生的荧光信号,通过熔解曲线可知其熔点值约为85.6℃,而对其他非沙门氏菌则检测不到荧光信号。建立了一种肉品中的沙门氏菌Real-time PCR... 应用SYBR Green I染料能选择性结合双链DNA的特点,可检测到沙门氏菌fimI基因特异性靶序列扩增所产生的荧光信号,通过熔解曲线可知其熔点值约为85.6℃,而对其他非沙门氏菌则检测不到荧光信号。建立了一种肉品中的沙门氏菌Real-time PCR检测方法,用该方法检测市售牛肉、香肠中的沙门氏菌,其检测灵敏度分别为13,12 cfu/25 g,从样品的处理到得出检验结果可以在10 h内完成。该检测方法具有简便、快速、特异性强、敏感度高等特点。 展开更多
关键词 沙门氏菌 real-time PCR 快速检测 肉品
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Real-time PCR技术的应用研究进展 被引量:6
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作者 李珊珊 王加启 +3 位作者 李旦 董晓丽 赵圣国 卜登攀 《生物技术通报》 CAS CSCD 北大核心 2009年第8期60-62,共3页
Real-time PCR(RT-PCR)是基于PCR,利用不同的荧光检测定量核酸的技术,广泛应用于基因分型,单核苷酸多态性,等位基因突变检测等方面。综述了RT-PCR作为一种检测技术,在医学、食品、环境微生物等不同领域的应用进展。
关键词 real-time PCR基因诊断 基因检测 基因表达
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Real-Time PCR探针法定量检测沙门菌方法的建立及应用 被引量:3
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作者 耿士忠 潘志明 +5 位作者 方强 丛秋霞 刘杰 刘志成 文志发 焦新安 《中国人兽共患病学报》 CAS CSCD 北大核心 2010年第11期1004-1007,1027,共5页
目的建立快速、特异性好、灵敏度高的Real-Time PCR方法定量检测沙门菌。方法根据编码沙门菌肠毒素基因stn的核苷酸序列,设计荧光探针和一对引物,通过对荧光定量PCR反应体系和反应条件的摸索,建立定量检测沙门菌的方法。结果建立的Real-... 目的建立快速、特异性好、灵敏度高的Real-Time PCR方法定量检测沙门菌。方法根据编码沙门菌肠毒素基因stn的核苷酸序列,设计荧光探针和一对引物,通过对荧光定量PCR反应体系和反应条件的摸索,建立定量检测沙门菌的方法。结果建立的Real-Ti me PCR方法有很好的特异性与敏感性,所检测沙门菌结果均为阳性,而非沙门菌均为阴性;标准曲线相关系数为R2=0.993,其敏感性为5CFU。运用该方法对108份鸡粪便、50份鸡肉以及58份水样进行检测,阳性率分别为3.7%(6/108)、4%(2/50)和3.4%(2/58),与传统细菌分离检测结果相符。结论结果表明该方法具有简便、快速、特异性强、敏感性高等特点,此研究为环境及疾病诊断中沙门菌快速检测提供了新方法。 展开更多
关键词 沙门菌 real-time PCR 荧光定量 快速检测
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猪圆环病毒2型SYBRGreen Real-time qPCR检测方法的建立 被引量:9
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作者 冯华 刘运超 +2 位作者 陈玉梅 魏蔷 张改平 《华北农学报》 CSCD 北大核心 2018年第4期98-103,共6页
为了建立一种基于SYBRGreen的猪圆环病毒2型(PCV2)荧光定量PCR诊断方法,以PCV2 ORF2基因序列为研究对象,构建p MD19T-ORF2重组质粒作为阳性标准品,设计特异性引物,对该方法的最适引物浓度、退火进行了优化,并对该方法的灵敏度、特异性... 为了建立一种基于SYBRGreen的猪圆环病毒2型(PCV2)荧光定量PCR诊断方法,以PCV2 ORF2基因序列为研究对象,构建p MD19T-ORF2重组质粒作为阳性标准品,设计特异性引物,对该方法的最适引物浓度、退火进行了优化,并对该方法的灵敏度、特异性、可重复性以及临床样品检出率进行了评价。结果表明,该方法引物的最佳终浓度为0. 2 mol/L,最适退火温度为55℃;检测限可达到3. 0×10~2个拷贝/mL,而普通PCR检测限在3. 0×10~4个拷贝/mL;溶解曲线分析显示,在77~79℃有单一的溶解峰出现,并与CSFV、PPV、PRRSV、PRV没有交叉反应;组内重复性变异系数为0. 29%~0. 32%,组间变异系数为0. 32%~0. 36%;此外,该方法对临床样品的检出率为81. 5%(31/38),而常规PCR为68. 4%(26/38)。因此,相比常规PCR,该方法更为快速、灵敏、特异、稳定,更适合PCV2临床样品的检测,可为该PCV2早期感染的快速检测提供新方法。 展开更多
关键词 猪圆环病毒2型 PCV2 ORF2 SYBRGreen real-time qPCR
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运用Real-time quantification PCR方法建立副溶血性弧菌在即食虾中的生长预测模型 被引量:5
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作者 彭织云 王敬敬 +2 位作者 唐晓阳 潘迎捷 赵勇 《食品工业科技》 CAS CSCD 北大核心 2013年第8期108-110,共3页
运用Real-time quantification PCR(real-time qPCR)方法建立副溶血性弧菌在即食虾中生长预测模型。首先构建质粒标准品,梯度稀释后建立标准曲线,然后用Real-time qPCR方法检测虾中副溶血性弧菌的数量,最后建立37℃下即食虾中副溶血性... 运用Real-time quantification PCR(real-time qPCR)方法建立副溶血性弧菌在即食虾中生长预测模型。首先构建质粒标准品,梯度稀释后建立标准曲线,然后用Real-time qPCR方法检测虾中副溶血性弧菌的数量,最后建立37℃下即食虾中副溶血性弧菌生长预测模型,并与传统涂布计数方法进行比较。结果表明,Real-time qPCR方法和传统计数方法均可建立Gmopertz模型、Logistic模型和Richards模型,模型拟合的相关系数R2均在0.9以上。基于Real-timeqPCR方法省时省力、特异性好等优点,用Real-time qPCR方法建立微生物预测模型是未来预测微生物学领域的一种发展趋势。 展开更多
关键词 real-time quantification PCR 副溶血性弧菌 生长预测模型
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利用SYBR Green检测衣原体Real-Time PCR方法的建立 被引量:2
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作者 杨建民 郝永新 +1 位作者 赵德明 何诚 《畜牧兽医学报》 CAS CSCD 北大核心 2006年第1期84-90,共7页
利用SYBR Green建立了检测种特异性衣原体的Real-Time PCR方法。本方法应用衣原体种特异性的高度保守特异引物,能够扩增627bp特异片段;使用定量标准基因组DNA,本方法能准确检测最少250fg衣原体DNA。Real-TimePCR方法与免疫荧光方法... 利用SYBR Green建立了检测种特异性衣原体的Real-Time PCR方法。本方法应用衣原体种特异性的高度保守特异引物,能够扩增627bp特异片段;使用定量标准基因组DNA,本方法能准确检测最少250fg衣原体DNA。Real-TimePCR方法与免疫荧光方法的检测结果表明:检测4种衣原体临床样本,Real-TimePCR敏感性均在96%~98%;特异性均为100%;这两种方法符合率达97%以上(n=60);批内和批间重复性试验结果表明,本方法具有良好的准确性。本方法的建立对于快速、准确检测临床样本种特异性衣原体提供了一种切实有效的方法。 展开更多
关键词 SYBR Green 衣原体 real-time PCR 检测
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