The study aimed to develop vanadium tea products and evaluated the potential of chelated vanadium(V)absorption by tea shoots and effect of vanadium accumulation on tea quality during the first crop season.Chelated V a...The study aimed to develop vanadium tea products and evaluated the potential of chelated vanadium(V)absorption by tea shoots and effect of vanadium accumulation on tea quality during the first crop season.Chelated V application with three times foliar sprays at 5 days intervals starting from 15 days before plucking showed a significant increase in V content of new young shoots,which was much effective at higher concentration.However,one or two times sprays had no increase in V content regardless of spray concentration compared to non-treated.Despite V accumulation in the treated at 3 to 9 times higher that of than the non-treated plants,there were no significant changes in contents of total amino acids,theanine,caffeine,total polyphenols,crude fiber,and cathechins between the treated and the non-treated.展开更多
该研究建立了一种精准测定不同样品中氨基酸含量及其氮同位素组成的方法。首先利用N-新戊酰基-O-异丙酯(NPP)对氨基酸进行衍生化,使其更适用于气相色谱分析。随后,采用气相色谱-质谱(GCMS)对大豆、土壤以及标准样品中的15种氨基酸单体...该研究建立了一种精准测定不同样品中氨基酸含量及其氮同位素组成的方法。首先利用N-新戊酰基-O-异丙酯(NPP)对氨基酸进行衍生化,使其更适用于气相色谱分析。随后,采用气相色谱-质谱(GCMS)对大豆、土壤以及标准样品中的15种氨基酸单体进行定量分析,所有目标氨基酸均获得良好的分离效果,且在1.0~16.0μmol/L浓度范围内呈线性关系(r^(2)>0.98)。此外,利用气相色谱-燃烧-同位素比值质谱(GC-C-IRMS)对上述样品中氨基酸的氮同位素组成(δ~(15)N)进行了测定。结果表明,当进样量超过20 ng N([N_(2)^(+)]m/z 28信号强度约为150 mV)时,该方法可以获得稳定可靠的δ^(15)N值,平均精度可达0.36‰。通过与元素分析-同位素比值质谱(EA-IRMS)的结果进行比较,两种方法的测定结果高度一致(r^(2)=0.9954),表明NPP衍生化过程未引入明显的氮同位素分馏。最终测得大豆和土壤样品中各氨基酸的δ~(15)N值分别分布在10.90‰~22.32‰和-1.92‰~12.82‰之间,标准偏差分别为0.23‰~0.88‰和0.08‰~0.79‰,符合样品分析的精度要求。展开更多
对以桑枝栽培的榆黄蘑子实体中蛋白质、氨基酸含量及相关指标进行分析和评价,检测得到桑枝榆黄蘑子实体蛋白质含量为26.60 g·100^(-1)·g^(-1),属于高蛋白食品;含有18种氨基酸,种类齐全,较接近FAO/WHO模式的标准;支链氨基酸和...对以桑枝栽培的榆黄蘑子实体中蛋白质、氨基酸含量及相关指标进行分析和评价,检测得到桑枝榆黄蘑子实体蛋白质含量为26.60 g·100^(-1)·g^(-1),属于高蛋白食品;含有18种氨基酸,种类齐全,较接近FAO/WHO模式的标准;支链氨基酸和芳香族氨基酸总量的比值为2.63;经氨基酸评分(amino acid score,AAS)、化学评分(chemical score,CS)、氨基酸比值系数评分(score of ratio coefficient of amino acid,SRCAA)3种指标评分,其第一限制氨基酸均为胱氨酸+蛋氨酸(Cys+Met);以FAO/WHO模式评价,桑枝榆黄蘑子实体所含蛋白质是优质蛋白,且营养价值较高。展开更多
基金support of "Cooperative Research Program for Agricultural Science & Technology Development (Project No.PJ0097432013)"Rural Development Administration,Republic of Korea
文摘The study aimed to develop vanadium tea products and evaluated the potential of chelated vanadium(V)absorption by tea shoots and effect of vanadium accumulation on tea quality during the first crop season.Chelated V application with three times foliar sprays at 5 days intervals starting from 15 days before plucking showed a significant increase in V content of new young shoots,which was much effective at higher concentration.However,one or two times sprays had no increase in V content regardless of spray concentration compared to non-treated.Despite V accumulation in the treated at 3 to 9 times higher that of than the non-treated plants,there were no significant changes in contents of total amino acids,theanine,caffeine,total polyphenols,crude fiber,and cathechins between the treated and the non-treated.
文摘该研究建立了一种精准测定不同样品中氨基酸含量及其氮同位素组成的方法。首先利用N-新戊酰基-O-异丙酯(NPP)对氨基酸进行衍生化,使其更适用于气相色谱分析。随后,采用气相色谱-质谱(GCMS)对大豆、土壤以及标准样品中的15种氨基酸单体进行定量分析,所有目标氨基酸均获得良好的分离效果,且在1.0~16.0μmol/L浓度范围内呈线性关系(r^(2)>0.98)。此外,利用气相色谱-燃烧-同位素比值质谱(GC-C-IRMS)对上述样品中氨基酸的氮同位素组成(δ~(15)N)进行了测定。结果表明,当进样量超过20 ng N([N_(2)^(+)]m/z 28信号强度约为150 mV)时,该方法可以获得稳定可靠的δ^(15)N值,平均精度可达0.36‰。通过与元素分析-同位素比值质谱(EA-IRMS)的结果进行比较,两种方法的测定结果高度一致(r^(2)=0.9954),表明NPP衍生化过程未引入明显的氮同位素分馏。最终测得大豆和土壤样品中各氨基酸的δ~(15)N值分别分布在10.90‰~22.32‰和-1.92‰~12.82‰之间,标准偏差分别为0.23‰~0.88‰和0.08‰~0.79‰,符合样品分析的精度要求。
文摘对以桑枝栽培的榆黄蘑子实体中蛋白质、氨基酸含量及相关指标进行分析和评价,检测得到桑枝榆黄蘑子实体蛋白质含量为26.60 g·100^(-1)·g^(-1),属于高蛋白食品;含有18种氨基酸,种类齐全,较接近FAO/WHO模式的标准;支链氨基酸和芳香族氨基酸总量的比值为2.63;经氨基酸评分(amino acid score,AAS)、化学评分(chemical score,CS)、氨基酸比值系数评分(score of ratio coefficient of amino acid,SRCAA)3种指标评分,其第一限制氨基酸均为胱氨酸+蛋氨酸(Cys+Met);以FAO/WHO模式评价,桑枝榆黄蘑子实体所含蛋白质是优质蛋白,且营养价值较高。
